|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Effect of As doping on the photoluminescence of nanocrystalline 74 Ge embedded in SiO 2 matrix显示文摘 | Shaobo Dun Tiecheng Lu Youwen Hu Qiang Hu Liuqi Yu Zheng Li Ningkang Huang Songbao Zhang Bin Tang Junlong Dai Lev Resnick Issai Shlimak | 2008 | Journal of Luminescence2008,,8: | 1 |
| 2 | The Raman spectroscopy of neutron transmutation doping isotope 74 Germanium nanocrystals embedded in SiO 2 matrix显示文摘 | Youwen Hu Tiecheng Lu Shaobo Dun Qiang Hu Ningkang Huang Songbao Zhang Bin Tang Junlong Dai Lev Resnick Issai Shlimak Sha Zhu Qiangmin Wei Lumin Wang | 2006 | Solid State Communications2006,,9: | 1 |
| 3 | Separation and Preconcen-Tration of Ultratrace Lead in Biological Organisms and its Determination by Graphite Furnace Atomic Absorption Spectrometry显示文摘 | Tang Youwen | 1996 | Talanta1996,43,: | 1 |
| 4 | Community-based study of the association of subclinical thyroid dysfunction with blood pressure显示文摘 | Yu Duan Wen Peng Xiaodong Wang Wei Tang Xiaoyun Liu Shuhang Xu Xiaodong Mao Shangyong Feng Yu Feng Youwen Qin Kuanfeng Xu Cuiping Liu Chao Liu | 2009 | Endocrine2009,,2: | 1 |
| 5 | Effect of IL-10 gene transmission on the PTg-stimulated splenocytes proliferation and Th1 cytokines production from experimental autoimminue thyroiditis rats显示文摘Objective: To investigate the effects of gene therapy with IL-10 on PTg-induced proliferation of splenocytes and Th1 cytokine production from PTg-stimulated splenocytes. Methods: EAT rats were divided into four groups:group A (PBS+PLL) , group B(pORF+PLL), group C (pORFmIL10+PLL), and group D (pORFmIL10+ MEM). The substances mixed with lipofectamine were injected into the thyroid tissues of rats on the 18th dday after immunization. The rats were sacrificed at the 8th week. In vitro proliferative responses to ConA and different concentration of PTg were measured by culturing 4×105 splenocytes pulsed with 18.5KBq of,?3H,Y thymidine for the final 12h and then harvested for liquid scintillation counting. In vitro splenocytes were cultured with PTg(25 mg/L). Th1 cytokine IFN-r,TNF-αand IL-2 were detected by ELISA. Results: The proliferative response to PTg was suppressed in group C, compared with that of group A and B(P < 0.05). The levels of IFN-r,TNF-αand IL-2 in the supernatant of PTg-stimulated splenocytes were 3548.25 ± 779.47 pg/ml, 27.66±10.50 pg/ml and 3617.73± 609.15 pg/ml, respectively, which were much lower in group C than those in group A and B(P < 0.01, P < 0.05,P < 0.001, respectively). Conclusion: IL-10 gene transmission in thyroid tissues could inhibit PTg specific proliferation of splenocytes from EAT rats and the secretion of Th1 cytokines from PTg-stimulated splenocytes. | Wei Tang Cuiping Liu Youwen Qin Yue Jia XiaodongMao Chao Liu | 2007 | Journal of Nanjing Medical University2007,21,1: | 1 |
| 6 | Imprinted functionalized silica sol-gel for solid-phase extraction of tri- azolamin显示文摘 | Guoyou Jin Baofei Zhang Youwen Tang | 2011 | Talanta2011,,84: | 1 |
| 7 | Fetal bovine serum versus Chinese herbal formula Naoluoxintong serum supplementation for proliferation and differentiation of rat embryonic neural stem cells显示文摘BACKGROUND:How to induce endogenous neural stem cells (NSCs) to differentiate into needed neural cell types is a hot spot of current researches.OBJECTIVE:To compare differences between fetal bovine serum and Chinese herbal formula Naoluoxintong serum supplementation for inducing proliferation and differentiation in rat embryonic NSCs.DESIGN, TIME AND SETTING:An in vitro, serum pharmacology, comparative, observation study was performed from March to September in 2008 at the Laboratory of Neurodegenerative Diseases, College of Life Science in University of Science and Technology of China, the Key Laboratory Breeding Base of Acupuncture Foundation and Technology in Anhui University of Traditional Chinese Medicine, the Anhui Province Key Laboratory of R & D of Chinese Medicine, and at the Level 3 Laboratory of Molecular Biology of the State Administration of Traditional Chinese Medicine.MATERIALS:The Chinese herbal formula Naoluoxintong was produced by Radix Astragali, Radix Notoginseng, Rhizoma Chuanxiong, Scolopendra at Anhui University of Traditional Chinese Medicine.Mouse anti-rat nestin, glial fibrillary acidic protein, and galactocerebroside monoclonal antibodies, as well as rabbit anti-neuron-specific enolase polyclonal antibody were produced by Chemicon, Billerica, MA, USA.METHODS:Wistar rats aged 3 months were intragastrically infused with Naoluoxintong.Wistar rat embryonic NSCs (passage 8) were induced to proliferate and differentiate using 10% fetal bovine serum, 10% Naoluoxintong serum, and 10% rat serum.MAIN OUTCOME MEASURES:Phenotypic changes in cultured cells were detected using phase contrast microscopy, and cell proliferation and differentiation were observed using immunofluorescence staining.RESULTS:Proliferation and differentiation of embryonic NSCs was induced by three different types of blood serum.Although the differentiation time course with Naoluoxintong serum was later than with the other two methods, the differentiated cells were morphologically similar to mature neurons to a greater extent.CONCLUSION:Naoluoxintong serum supplementation induced differentiation of NSCs into neuronal-like cells and stimulated neuronal maturation. | Wei Tang Jian Wang Youwen Wang Chaomin Ni Yenong Chen Zhaoliang Tang Lihua Yu Xiaomin Li Jianpeng Hu | 2010 | Neural Regeneration Research2010,5,14: | 0 |