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| 1 | Activating mechanism of transcriptor NF-kappaB regulated by hepatitis B virus X protein in hepatocellular carcinoma显示文摘AIM:To investigate the mechanism and significance of NF-κB activation regulated by hepatitis B virus X protein (HBx) in hepatitis B virus (HBV)-associated hepatocellular carcinoma (HCC).METHODS:The expression levels of HBx, p65,IκB-α and ubiquitin were detected by immunohistochemistry in HCC tissue microarrays (TMA) respectively, and IκB-α was detected by Western blot in HCC and corresponding liver tissues.RESULTS: The percentage of informative TMA samples was 98.8% in 186 cases with a total of 367 samples. Compared with corresponding liver tissues (60.0%),the HBx expression was obviously decreased in HBV-associated HCC (47.9%,u=2.24,P<0.05).On the contrary, the expressions of p65 (20.6% vs45.3%, u=4.85, P<0.01) and ubiquitin (8.9% vs 59.0%,u=9.68,P<0.01) were notably elevated in HCC.In addition, IκB-α had a tendency to go up. Importantly, positive relativity was observed between HBx and p65 (X^2=10.26,P<0.01), p65 and IκB-α (x^2=16.86,P<0.01), IκB-α and ubiquitin (x^2=8.90, P<0.01) in HCC, respectively.CONCLUSION:Both active and non-active forms of NF-κB are increased in HBV-associated HCC. Variant HBx is the major cause of the enhancement of NF-κB activity. The activation always proceeds in nucleus and the proteasome complexes play an important role in the activation. | TaoWang YiWang Meng-ChaoWu Xin-YuanGuan Zheng-FengYin | 2004 | World Journal of Gastroenterology2004，10，3： | 36 |
| 2 | Down-modulation of heat shock protein 70 and up-modulation of Caspase-3 during schisandrin B-induced apoptosis in human hepatoma SMMC-7721 cells显示文摘AIM: To investigate the effect of schisandrin B (Sch B) on proliferation and apoptosis of human hepatoma SMMC-7721 cells in vitro and regulation of Hsp70 and Caspases-3, 7, 9 expression by Sch B. METHODS: Human hepatoma cell line SMMC-7721 was cultured and treated with Sch B at various concentrations. Growth suppression was detected with MTT colorimetric assay. Cell apoptosis was confirmed by DNA ladder detection and flow cytometric analysis. The expression of Hsp70, Caspases-3, 7, 9 were analyzed by Western blot analysis. RESULTS: Sch B inhibited the growth of hepatoma SMMC-7721 cells in a dose-dependent manner, leading to a 50% decrease in cell number (LC50) value of 23.50 mg/L. Treatment with Sch B resulted in degradation of chromosomal DNA into small internucleosomal fragments, evidenced by the formation of a 180-200 bp DNA ladder on agarose gels. FCM analysis showed the peak areas of subdiploid at the increased concentration of Sch B. The results of Western bolt analysis showed that Hsp70 was down-regulated and Caspase-3 was up-regulated, while the activity of Caspases-7,-9 had no significant change. CONCLUSION: Sch B is able to inhibit the proliferation ofhuman hepatoma SMMC-7721 cells and induce apoptosis, which goes through Caspase-3-dependent and Caspase-9-independent pathway accompanied with the down-regulation of Hsp70 protein expression at an early event. | Yi-FengWu Ming-FuCao Yan-PingGao FeiChen TaoWang EdwardP.Zumbika Kai-XianQian | 2004 | World Journal of Gastroenterology2004，10，20： | 29 |
| 3 | Honokiol induces apoptosis through p53-independent pathway in human colorectal cell line RKO显示文摘AIM: To investigate the signal pathway of honokiol-induced apoptosis on human colorectal carcinoma RKO cells and to evaluate whether p53 and p53-related genes were involved in honokiol-treated RKO cells.METHODS: Cell cycle distribution and subdiploid peak were analyzed with a flow cytometer and DNA fragment with electrophoresis on agarose gels. Transcriptional level of Bax, Bcl-2, Bid and Bcl-xl was accessed by RT-PCR.Western blotting was used to measure p53 protein expression and other factors related to apoptosis.Proliferation inhibition of two cell lines (RKO, SW480) with high expression of p53 and one cell line with p53 negative expression (LS180) was monitored by MTr assay.RESULTS: Honokiol induced RKO cell apoptosis in a dosedependent manner. The mRNA expression level and proteinlevel of Bid were up-regulated while that of Bcl-xl wasdown-regulated, but no changes in Bax and Bcl-2 were observed. Western blotting showed p53 expression had no remarkable changes in honokiol-induced RKO cell apoptosis. LS180 cells treated with honokiol exhibited apparent growth inhibition like RKO ceils and Sw480 ceils.CONCLUSION: Honokiol can induce RKO cells apoptosis through activating caspase cascade by p53-indepenent pathway. | TaoWang FeiChen ZheChen Yi-FengWut Xiao-LiXu ShuZheng XunHu | 2004 | World Journal of Gastroenterology2004，10，15： | 27 |
| 4 | Honokiol:A potent chemotherapy candidate for human colorectal carcinoma显示文摘AIM: To investigate the anticancer activity of Honokiol on RKO,a human colorectal carcinoma cell line in vitro and in vivo,and to evaluate its possible use in clinic.METHODS: In vitro anticancer activity of honokiol was demonstrated by its induction of apoptosis in tumor cells.We analyzed cell proliferation with MTT assay, cell cycle with flow cytosmeter, DNA fragment with electrophoresis on agarose gels. To test the mechanism of honokiol-induced apoptosis, Westem blotting was used to investigate the factors involved in this process. The pharmacokinetics study of honokiol was tested by high phase liquid chromatography.In in vivo study, Balb/c nude mice were incubated with RKO cells. Honokiol was injected intraperitoneally every other day into tumor bearing Balb/c nude mice.RESULTS: Our results showed that honokiol induced apoptosis of RKO cells in a time- and dose-dependent manner. At 5-10 ug/mL for 48 h, honokiol induced apoptosis through activating Caspase cascades. Pharmacokinetics study demonstrated that, honokiol could be absorbed quickly by intraperitoneal injection, and maintained in plasma for more than 10 h. In nude mice bearing RKO-incubated tumor, honokiol displayed anticancer activity by inhibiting tumor growth and prolonging the lifespan of tumor bearing mice.CONCLUSION: With its few toxicity to normal cells and potent anticancer activity in vitroand in vivo, honokiol might be a potential chemotherapy candidate in treating human colorectal carcinoma. | FeiChen TaoWang Yi-FengWu YingGu Xiao-LiXu ShuZheng XunHu | 2004 | World Journal of Gastroenterology2004，10，23： | 23 |
| 5 | Microvessel density of malignant and benign hepatic lesions and MRI evaluation显示文摘AIM. To study the difference of microvessel density (MVD) between malignant and benign hepatic lesions and study the relationship between MVD and dynamic enhanced magnetic resonance imaging (MRI) for evaluation of microvessels within malignant and benign hepatic lesions. METHODS: A total of 265 specimens of hepatocellular carcinoma (HCC), 122 cirrhosis tissues and 22 hepatic benign lesions were enrolled for MVD by immunohistochemistry on tissue microarray, of which 49 underwent MRI examination before surgery, then contrast-to-noise ratios (CNR) and enhancement index (EI) in all the phases were calculated. Pearson correlation was performed for correlation analysis between CNR, EI and MVD. RESULTS: MVD of HCC was 22.7±15.8 (mean±SD), which was obviously higher than that of cirrhosis tissue (8.3±7.6, P<0.01), but was not statistically different from that of benign lesions (31.3±22.7, P>0.05). Among HCC, MVD of gradesⅠ-Ⅱ was 29.9±18.6, which was much higher than those ofgrade Ⅲ (22.2±18.2, P<0.01) and gradeⅣ (22.9±19.0, P<0.01). MVD of HCC (P=0.018) and of benign lesions (P=0.014) were both correlative with CNR in arterial phase. CONCLUSION: Neoangiogenesis is an important feature for malignant tumor, and MVD may act as a biological marker in differentiating malignant from benign hepatic lesions. Dynamic enhanced MRI, especially image in arterial phase, may act as an MVD evaluation criterion for malignant and benign hepatic lesions. | Jian-PingLu JianWang TaoWang YiWang wei-Qingwu LiGao | 2004 | World Journal of Gastroenterology2004，10，12： | 13 |
| 6 | Simple Sequence Repeat Analysis of Genetic Diversity in Primary Core Collection of Peach(Prunus persica)显示文摘In this study,the genetic diversity of 51 cultivars in the primary core collection of peach(Prunus persica(L.) Batsch) was evaluated by using simple sequence repeats(SSRs) . The phylogenetic relationships and the evolutionary history among different cultivars were determined on the basis of SSR data. Twenty-two polymorphic SSR primer pairs were selected,and a total of 111 alleles were identified in the 51 cultivars,with an average of 5 alleles per locus. According to traditional Chinese classification of peach cultivars,the 51 cultivars in the peach primary core collection belong to six variety groups. The SSR analysis revealed that the levels of the genetic diversity within each variety group were ranked as Sweet peach > Crisp peach > Flat peach > Nectarine > Honey Peach > Yellow fleshed peach. The genetic diversity among the Chinese cultivars was higher than that among the introduced cultivars. Cluster analysis by the unweighted pair group method with arithmetic averaging(UPGMA) placed the 51 cultivars into five linkage clusters. Cultivar members from the same variety group were distributed in different UPGMA clusters and some members from different variety groups were placed under the same cluster. Different variety groups could not be differentiated in accordance with SSR markers. The SSR analysis revealed rich genetic diversity in the peach primary core collection,representative of genetic resources of peach. | Tian-Hong Li Yin-Xia Li Zi-Chao Li Hong-Liang Zhang Yong-Wen Qi TaoWang | 2008 | Journal of Integrative Plant Biology2008，50，1： | 8 |
| 7 | Helicobacterpylori infection and gastric cancer:evidence from a retrospective cohort study and nested case-control study in China显示文摘AIM:To explore the association between Helicobacter pylri(HP) infection and risk of gastric cancer in China. | Bing-QuanWu TaoWang KunChen Ji-YaoWang Jie-PingZhang San-RenLi | 2002 | World Journal of Gastroenterology2002，8，6： | 8 |
| 8 | Differences in biological features of gastric dysplasia, indefinite dysplasia, reactive hyperplasia and discriminant analysis of these lesions显示文摘AIM: To investigate the differences in biological features of gastric dysplasia (Dys), indefinite dysplasia (IDys) and reactive hyperplasia (RH) by studying the biomarker alterations in cell proliferation, cell differentiation, cell cycle control and the expression of house-keeping genes, and further to search for markers which could be used in guiding the pathological diagnosis of three lesions. METHODS: Expressions of MUC5AC, MUC6, adenomatous polyposis coli (APC), p53, Ki-67, proliferation cell nuclear antigen (PCNA) and EGFR were studied by immunohistochemistry with a standard Envision technique in formalinfixed and paraffin-embedded specimens from 43 RH, 35 IDys, 35 Dys and 36 intestinal type gastric carcinomas (IGC). In addition, Bayes discriminant analysis was used to investigate the value of markers studied in differential diagnosis of RH, IDys, Dys and IGC. RESULTS: The MUC5AC and MUC6 antigen expressions in RH, IDys, Dys and IGC decreased gradually (MUC5AC:86.04%, 77.14%, 28.57%, 6.67%; MUC6: 65.15%, 54.29%, 20.00%, 25.00%, respectively). The expressions of the two markers had no significant difference between RH and IDys, but were all significantly higher than those ofthe other two lesions (MUC5AC: x2 = 27.607, 38.027 and 17.33, 26.092; MUC6: x2= 16.54, 12.665 and 9.282, 6.737, P<0.01). There was no significant differencebetween RH and IDys, Dys and IGC in MUC6 expression. The APC gene expression in the four lesions had a similar decreasing tendency (RH 69.76%, IDys 68.57%, Dys39.39%, IGC 22.86%), and it was significantly higher in the first two lesions than in the last two (x2 = 7.011,16.995 and 14.737, 19.817, P<0.05). The p53 expressionin RH, IDys, Dys and IGC was 6.98%, 20%, 57.14% and 50%, respectively. There was no significant differencebetween RH and IDys or Dys and IGC, but the p53 expression in RH and IDys was significantly lower than that in Dys and IGC (x2 = 7.011, 16.995 and 14.737, 19.817, P<0.01).The Ki-67 label index was significantly different among four lesions (RH: 0.298±8.92%, IDys: 0.358±9.25%,Dys: 0.498±9.03%, IGC: 0.620±10.8%, P<0.001). Positive immunostaining of PCNA was though observed in all specimens, significant differences were detected among four lesions (F= 95.318, P<0.01). In addition, we used Bayes discriminant analysis to investigate molecular pathological classification of the lesions, and obtained the best result with the combination of MUC5AC, Ki-67 and PCNA. The overall rate of correct classification was67.4% (RH), 68.6% (IDys), 70.6% (Dys) and 84.8% (IGC), respectively.CONCLUSION: Dys has neoplastic biological characteristics, while RH and IDys display hyperplastic characteristics. MUC5AC and proliferation-related biomarkers (Ki-67, PCNA) are more specific in distinguishing Dys from RH and IDys. | BinDong Yu-QuanXie KeChen TaoWang WeiTang Wei-ChengYou Ji-YouLi | 2005 | World Journal of Gastroenterology2005，11，23： | 7 |
| 9 | Co-expression and Immunity of Legionella pneumophila mip Gene and Immunoadjuvant ctxB Gene显示文摘The mip gene of Legionella pneumophila and the ctxB gene of Vibrio cholerae were amplified by PCR respectively. The amplified cDNA was ligated to the pcDNA3.1(+) vector. The recombinant plasmids pcDNA3.1-mip and pcDNA3.1-ctxB were identified by restriction analysis and PCR, and further confirmed by sequencing analysis. NIH3T3 cells were transfected with pcDNA3.1-mip and pcDNA3.1-ctxB according to the Lipofection method. Transient and stable products of the co-expression of the mip gene and ctxB gene were detected by immunofluorescence and Western blotting. The results showed that NIH3T3 cells were successfully transfected, and that the transiently and stably co-expressed products can be detected in the transfected cells. To detect the humoral and cellular immune response in immunized mice induced by the coimmunization of the mip and ctxB genes, female BALB/c mice were immunized intramuscularly with pcDNA3.1-mip and pcDNA3.1-ctxB. The results showed that the specific antibody titer and the cytotoxic T-lymphocyte response for pcDNA3.1-mip immunization and co-immunization were increased compared with that of pcDNA3.1(+) immunization. Furthermore, the specific antibody titer and cytotoxic T-lymphocyte response for co-immunization were increased compared with that of pcDNA3.1-mip immunization. Statistical analysis using one-way analysis of variance (ANOVA) showed that there was a significant difference between the groups (P<0.01). The results indicated that the ctxB gene enhanced the humoral and cellular immune response to the mip gene immunization. These findings provide experimental evidence to support the development of the L. pneumophila DNA vaccine. | TaoWANG Jian-PingCHEN HongLI Ke-QianZHI LeiZHANG Chun-LeiYANG Da-ChangTAO | 2005 | Acta Biochimica et Biophysica Sinica2005，37，3： | 4 |
| 10 | Observa- tions on the ductile-to-brittle transition in ultrafine-grained tungsten of commercial purity显示文摘 | Zhang Yue Artur V Ganeev Jing TaoWang | 2009 | Mater Sci Eng A2009，503，： | 1 |
| 11 | Gene expression in Barrett's esophagus and reflux esophagitis induced by gastroduodenoesophageal reflux in rats显示文摘AIM: To investigate the difference of gene expression profiles between Barrett's esophagus and reflux esophagitis induced by gastroduodenoesophageal reflux in rats.METHODS: Eight-week-old Sprague-Dawley rats were treated esophagoduodenostomy to produce gastroduodenoesophageal reflux, and another group received sham operation as control. Esophageal epithelial tissues were dissected and frozen in liquid nitrogen immediately for pathology 40 wk after surgery. The expression profiles of 4 096 genes in reflux esophagitis and Barrett's esophagus tissues were compared with normal esophageal epithelium by cDNA microarray.RESULTS: Four hundred and forty-eight genes in Barrett'sesophagus were more than three times different from those in normal esophageal epithelium, including 312 up regulated and 136 down-regulated genes. Two hundred and thirty-twogenes in RE were more than three times different from those in normal esophageal epithelium, 90up-regulated and 142 down-regulated genes. Compared to reflux esophagitis, there were 214 up-regulated and 142 down-regulated genes in Barrett's esophagus. CONCLUSION: Esophageal epithelium exposed excessively to harmful ingredients of duodenal and gastric reflux can develop esophagitis and Barrett's esophagus gradually.The gene expression level is different between reflux esophagitis and Barrett's esophagus and the differentially expressed genes might be related to the occurrence and development of Barrett's esophagus and the promotion or progression in adenocarcinoma. | PengCheng JunGong TaoWang ChenJie GuiShengLiu RuZhang | 2005 | World Journal of Gastroenterology2005，11，21： | 1 |
| 12 | Preparation of silica aerogel from rice hull ash by drying at atmospheric pressure 显示文摘 | Ting TaoWang | 2008 | Materials Chemistry and Physics2008，，4： | 1 |
| 13 | Recombinant adeno-associated virus-mediated delivery of antisense angiotensin Ⅱ receptor 1 gene attenuates hypertension development显示文摘目的: 高血压蛋白原酶血管收缩素系统在高血压的开发和建立起一个关键作用,并且系统的药理学封锁在血压导致减小。在现在的学习,我们调查了是否一篇小说的效果,双 stranded, recombinant 联系 adeno 的病毒向量(rAAV ) 调停了反感觉血管收缩素 II 受体 1 (AT1R ) 基因高效地阻止一本高盐的食谱在成年、男 Sprague-Dawley (SD ) 老鼠导致的高血压的发展。方法: rAAV 与为在反感觉方向(rAAV-AT1-AS ) 插入的 AT1R 包含一个 cytomegalovirus 倡导者和部分 cDNA (660 基础对) 的一个盒子被准备。rAAV-AT1-AS 或 rAAV-GFP 的单个尾巴静脉注射(绿荧光灯的蛋白质,记者基因) 在成年、男 SD 老鼠被执行。在注射以后的二个星期,动物被喂包含 8% NaCl 的一本食谱,和收缩血压用尾巴手铐方法每周被测量 12 个星期。结果:高盐的食谱在 rAAV-GFP-treated 动物在收缩血压导致了重要上升;然而, rAAV-AT1-AS 处理在血压稀释了上升(142.7+/-4.5 毫米汞柱对 117+/-3.8 毫米汞柱, P<0.01 ) ,并且低血压患者效果被维持直到实验在 12 个星期结束了。在 rAAV-GFP-treated 动物 AT1 完了在各种各样的纸巾表示了,特别在在 mRNA 层次的主动脉和肾;相反, rAAV-AT1-AS 处理显著地稀释了 AT1 表示。而且, rAAV-AT1-AS 治疗阻止了目标机关损坏高血压,包括与 rAAV-GFP 组相比的心脏的机能障碍和肾损伤。结论: 这些结果建议那 rAAVmediated anti-AT1 交货稀释高血压的发展并且免于肾损伤并且心脏的改变。 | Xu-guang LI Jiang-tao YAN Xi-zheng XU Jia-ning WANG Li-ming CHENG TaoWANG Ping ZUO Dao-wen WANG | 2007 | Acta Pharmacologica Sinica2007，28，11： | 1 |
| 14 | InfluenceofregionalpollutionoutflowontheconcentrationsoffineparticulatematterandvisibilityinthecoastalareaofsouthernChina显示文摘 | Hing-ChoCheng TaoWang KarsternBaumann etal | 2005 | AtmosphericEnvironment2005，39，24： | 1 |
| 15 | Colchicine-inducedapoptosisin human normalliver L-02 cells by mitochondrialmediatedpathways显示文摘 | XuemeiChen JunLiu TaoWang etal | 2012 | ToxicologyinVitro2012，26，5： | 1 |
| 16 | Effect of shape of Fe3Al particles on their microwave permeability and absorption properties显示文摘 | Jianqiang Wei TaoWang Fashen Li | | 0，，21： | 1 |
| 17 | Intelligent predicting approach of peritoneal fluid absorption rate based-on neural network显示文摘This paper addresses the important intelligent predicting problem of peritoneal absorption rate in the peritoneal dialysis treament process of renal failure. As the index of dialysis adequacy, KT/V and Ccr are widely used and accepted. However,growing evidence suggests that the fluid balance may play a critical role in dialysis adequacy and patient outcome. Peritoneal fluid absorption decreases the peritoneal fluid removal. Understanding the peritoneal fluid absorption rate will help clinicians to opthnize the dialysis dwell time. The neural network approach is applied to the prediction of peritoneal absorption rate. Compared with multivariable regression method, the experimental results showed that neural network method has an advantage over multivariable regression. The application of this predicting method based-on neural network in clinic is instructive. | MeiZHANG YuemingHU TaoWANG | 2003 | 控制理论与应用(英文版)2003，1，1： | 1 |
| 18 | Foxp3<sup>+</sup> regulatory T cells are associated with the natural history of chronic hepatitis B and poor prognosis of hepatocellular carcinoma显示文摘 | XiangJing TaoWang ZhengyanZhu QinZhang YijunWang ZhiDu | 2012 | Liver Int2012，，4： | 1 |
| 19 | Cloning and expression of mouse peroxiredoxin I in 1EC-6 Cells显示文摘AIM: To clone and express mouse peroxiredoxin Ⅰ in IEC-6 cells.METHODS: Total RNAs were isolated from cultured IEC-6 cells, and the coding region of peroxiredoxin I was amplified by RT-PCR. After it was cloned into T-vector and sequenced,pSG5 was used to transiently express peroxiredoxin I in IEC-6 by liposome-mediated transfection, and the expression of peroxiredoxin I was evaluated by RT-PCRand Western blot. RESULTS: A DNA fragment about 750 bp was amplified from total RNAs of IEC-6 cells using specific primers of peroxiredoxin Ⅰ. The sequencing confirmed the coding region was successfully cloned into T-vector, which was completely coincident with the sequence in GeneBank. After the EcoRI-BamHI fragment of T-vector containing peroxiredoxin I was inserted into pSG5, the recombinant plasmid was transferred to IEC-6 cells. RT-PCR assay showed that a DNA fragment of 930 bp could be amplified,which indicated the transcription of pSG5-Prx. Western blot confirmed the expression of peroxiredoxin Ⅰ in IEC-6 cells.CONCLUSION: Mouse peroxiredoxin I can be successfully expressed in IEC-6 cells. | BoZhang Yong-PingSu TaoWang Feng-ChaoWang Guo-PingAi HuiXu Jun-PingWang Yue-ShengHuang Jian-XinJiang | 2004 | World Journal of Gastroenterology2004，10，14： | 1 |
| 20 | Glutathione S‐transferase polymorphisms are associated with survival in anaplastic glioma patients显示文摘 | LindsayKilburn M. FatihOkcu TaoWang YumeiCao AmyRenfro‐Spelman Kenneth D.Aldape Mark R.Gilbert MelissaBondy | 2010 | Cancer2010，，9： | 1 |
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