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| 1 | Feeding of Riptortus pedestris on soybean plants, the primary cause of soybean staygreen syndrome in the Huang-Huai-Hai river basin显示文摘Staygreen syndrome or Zhengqing in soybean has recently become a major issue for Chinese growers in the Huang-Huai-Hai river basin.Although previous studies revealed that staygreen can be induced when pods/seeds are damaged, it is unknown whether virus infection or insect infestation causes staygreen.To determine whether viral infection causes staygreen, a survey of soybean staygreen incidence in the Huang-Huai-Hai river basin was conducted in 2016 and 2017.Diseased samples were collected and analyzed using DAS-ELISA for Soybean mosaic virus, Watermelon mosaic virus, Bean pod mottle virus, Cucumber mosaic virus, and Bean common mosaic virus.The survey showed that the severity of soybean staygreen syndrome was most prevalent in Beijing, Henan, Shaanxi, and some parts of Shandong provinces, with yield losses from 0 to nearly 100%, but only a small fraction of samples were positive for the tested viruses.A field cage experiment and an insecticide treatment field trial were conducted to determine the contribution of the bean bug, Riptortus pedestris, to staygreen incidence.The field cage experiment showed that R.pedestris treatment resulted in shorter plants, more empty pods, increased numbers of abnormal seeds, and decreased yields.The field experiment showed that there were fewer R.pedestris and less soybean staygreen incidence in fields treated with insecticide than in untreated control fields.Together, these results suggest that R.pedestris infestation rather than virus infection induces staygreen syndrome and that growers in this region can mitigate staygreen syndrome via bean bug control. | Kai Li Xinxin Zhang Jianqiu Guo Hannah Penn Tingting Wu Lin Li Hong Jiang Lidan Chang Cunxiang Wu Tianfu Han | 2019 | The Crop Journal2019，7，3： | 30 |
| 2 | Physiological and molecular studies of staygreen caused by pod removal and seed injury in soybean显示文摘Leaves provide substances and signals for pod and seed development in soybean.However,the regulatory feedbacks of pod and seed to leaf development remain unclear.We investigated the effects of pod and seed on leaf senescence by conducting pod removal and seed injury experiments.Pod removal and seed injury delayed leaf senescence and caused the staygreen phenotype of leaves.There were dosage effects of pod number on the extent of staygreen in depodded plants.The concentrations of chlorophyll(SPAD value,an index of relative chlorophyll content),soluble protein,and soluble sugar in the leaves of depodded plants were higher than those of intact plants.During seed development,the content of IAA decreased,while that of ABA increased.This trend was more pronounced in intact than in depodded and seed-injured plants.The GA3/ABA ratio decreased gradually in all treatments.The content of GA3 was relatively stable and was higher in intact than in depodded plants.The expression levels of four senescence-related genes,Gm SARK,Gm SGR1,Gm CYN1,and Gm NAC,declined in depodded or seed-injured treatments and were positively correlated with the number of leaves retained on plants.Gm FT2 a,the major flowering-promoting gene,was expressed at a higher level while E1,a key flowering inhibitory gene,was expressed at a lower level in depodded than in intact plants.We propose that the pod or seed can regulate leaf development.When the seed is aborted owing to disease infection or pest attack,the leaves stay green because of the absence of the seed signals for senescence. | Xinxin Zhang Min Wang Tingting Wu Cunxiang Wu Bingjun Jiang Changhong Guo Tianfu Han | 2016 | The Crop Journal2016，4，6： | 23 |
| 3 | A Single Nucleotide Deletion in J Encoding GmELF3 Confers Long Juvenility and Is Associated with Adaption of Tropic Soybean显示文摘 | Yanlei Yue Nianxi Liu Bingjun Jiang Mu Li Haijie Wang Ze Jiang Huanting Pan Qiuju Xia Qibin Ma Tianfu Han Hai Nian | 2017 | Molecular Plant2017，10，4： | 20 |
| 4 | A new distinct geminivirus causes soybean stay-green disease显示文摘Dear Editor,Plant viruses make up almost half of the plant disease-causing pathogens,affecting crop yields and the global economy(Savary et al.,2019).Soybean(Glycine max)is one of the most valuable legume crops in the world,supplying 25%of the global edible oil and two-thirds of the global concentrated protein for livestock feeding.Recently,the outbreak of soybean stay-green syndrome with delayed leaf senescence(stay-green),flat pods,and increased number of abnormal seeds has swept the soybean production in the Huang-Huai-Hai region of China,resulting in huge yield losses(Xu et al.,2019).This disease has become an epidemic and prominent problem in soybean production and is still expanding its geography,including North America,posing a serious threat to soybean production(Harbach et al.,2016;Zhang et al.,2016;Li et al.,2019).However,the cause of soybean stay-green syndrome remains obscure. | Ruixiang Cheng Ruoxin Mei Rong Yan Hongyu Chen Dan Miao Lina Cai Jiayi Fan Gairu Li Ran Xu Weiguo Lu Yu Gao Wenwu Ye Shuo Su Tianfu Han Junyi Gai Yuanchao Wang Xiaorong Tao Yi Xu | 2022 | Molecular Plant2022，15，6： | 6 |
| 5 | Soybean hairy roots produced in vitro by Agrobacterium rhizogenes-mediated transformation显示文摘Soybean is one of the world's most important oil and protein crops. Efficient transformation is a key factor for the improvement of soybean by genetic modification. We describe an optimized protocol for the Agrobacterium rhizogenes-mediated transformation of soybean and the induction of hairy root development in vitro. Cotyledons with 0.5-cm hypocotyls were cut from 5-day-old seedlings and used as explants. After infection and co-cultivation,hairy roots were produced in induction culture medium after 10–12 days. Using this method, 90%–99% of the infected explants of five different cultivars produced hairy roots within one month. Observations using reporter constructs showed that 30%–60% of the hairy roots induced were transformed. Based on high transformation efficiency and short transformation period, this method represents an efficient and rapid platform for study of soybean gene function. | Li Chen Yupeng Cai Xiujie Liu Chen Guo Shi Sun Cunxiang Wu Bingjun Jiang Tianfu Han Wensheng Hou | 2018 | The Crop Journal2018，6，2： | 4 |
| 6 | Spatial differences in soybean bioactive components across China and their influence by weather factors显示文摘Bioactive components are partially responsible for the nutritional and health benefits of soybeans. Four major bioactive components: isoflavones, oligosaccharides, phospholipids,and saponins, were quantified in 763 soybean samples collected from widely distributed regions across China from 2010 to 2013. A majority of the tested bioactive components showed generally declining trends from the north(high latitude) to the south(low latitude).A positive relationship between total oligosaccharides(TO) and altitude was observed. Total isoflavones(TI), phospholipids(TP) and TO were negatively correlated with cumulative temperature above or equal to 15 °C(AT15) and mean daily temperature(MDT), but positively correlated with diurnal temperature range(DTR) and hours of sunshine(HS).Total saponins(TS) were negatively correlated with MDT but positively correlated with rainfall(RF), whereas TO were negatively correlated with RF. Path-coefficient analysis showed that, besides genotype differences, temperature and HS during the reproductive period influenced TI and TP contents, while temperature and RF influenced TS and TO. The effects of weather factors on soybean bioactive components in diverse regions of China were characterized. These findings will be helpful in promoting soybean production for functional food purposes. | Wenwen Song Ruping Yang Xiushi Yang Shi Sun Srinivasa Rao Mentreddy Bingjun Jiang Tingting Wu Shiyan Tian Enoch Sapey Cunxiang Wu Wensheng Hou Guixing Ren Tianfu Han | 2018 | The Crop Journal2018，6，6： | 3 |
| 7 | Nonlinear model of space manipulator joint considering time-vari- ant stiffness and backlash显示文摘 | Yang Tianfu Yah Shaoze Han Zengyao | 2015 | Journal of Sound and Vibration2015，341，： | 1 |
| 8 | Genomic dissection of widely planted soybean cultivars leads to a new breeding strategy of crops in the post-genomic era显示文摘Soybeans specially the widely planted cultivars have been dramatically improved in agronomic performance and is well adapted to local planting environments after long-time domestication and breeding.Uncovering the unique genomic features of popular cultivars will help to understand how soybean genomes have been modified through breeding.We re-sequenced 134 soybean cultivars that were released and most widely planted over the last century in China.Phylogenetic analyses established that these cultivars comprise two geographically distinct sub-populations:Northeast China (NE) versus the HuangHuai-Hai River Valley and South China (HS).A total of 309 selective regions were identified as being impacted by geographical origins.The HS sub-population exhibited higher genetic diversity and linkage disequilibrium decayed more rapidly compared to the NE sub-population.To study the association between phenotypic differences and geographical origins,we recorded the vegetative period under different growing conditions for two years,and found that clustering based on the phenotypic data was closely correlated with cultivar geographical origin.By iteratively calculating accumulated genetic diversity,we established a platform panel of cultivars and have proposed a novel breeding strategy named 'Potalaization'for selecting and utilizing the platform cultivars that represent the most genetically diversity and the highest available agronomic performance as the 'plateau'for accumulating elite loci and traits,breeding novel widely adapted cultivars,and upgrading breeding technology.In addition to providing new genomic information for the soybean research community,the 'Potalaization'strategy that we devised will also be practical for integrating the conventional and molecular breeding programs of crops in the post-genomic era. | Xinpeng Qi Bingjun Jiang Tingting Wu Shi Sun Caijie Wang Wenwen Song Cunxiang Wu Wensheng Hou Qijian Song Hon-Ming Lam Tianfu Han | 2021 | The Crop Journal2021，9，5： | 1 |
| 9 | History, current status, and prospects of soybean production and research in sub-Saharan Africa显示文摘Soybean(Glycine max(L.) Merr.) is a non-native and non-staple crop in sub-Saharan Africa(SSA) with potential to be a commercial crop owing to its wide range of uses as food, feed,and industrial raw material. Soybean was first introduced to SSA by Chinese traders in the19 th century and was cultivated as an economic crop as early as 1903 in South Africa. In the past four decades, soybean cultivation area and production in SSA has increased exponentially, from about 20,000 ha and 13,000 t in the early 1970 s to 1,500,000 ha and2,300,000 t in 2016. Soybean yield has been stagnant in SSA for decades at about 1.1 t ha^(-1),much lower than the world average, representing one of the most challenging issues in the soybean industry in SSA. The low soybean yield in SSA can be attributed to the use of poorperforming varieties and to the limited application of fertilizers and rhizobial inoculants in soils with no history of soybean production. South Africa, Nigeria, Zambia, and Uganda are the leading soybean producers in SSA. Soybean research in SSA is conducted by international and national research institutions, including IITA, national soybean improvement programs, universities, and the private sector. Between 1970 and 2011, 195 soybean varieties were released by IITA, private breeders, and national soybean improvement programs in SSA. This paper reviews the history and current state of soybean production and of the utilization and adoption of tropical varieties in SSA, addresses the major soybean yield-limiting factors across the region, and discusses the potential of the soybean industry in SSA. It also highlights soybean improvement efforts and lessons learned from previous soybean improvement efforts and the current progress of some national soybean improvement programs in SSA. Opportunities for scaling up tropical soybean as a major crop across SSA countries are promising. | Dalia Mohamedkheir Khojely Seifeldin Elrayah Ibrahim Enoch Sapey Tianfu Han | 2018 | The Crop Journal2018，6，3： | 1 |
| 10 | Improved Preproduction Conceptual-Numerical Model of the Roosevelt Geothermal Field using Geological, Geochemical and Structural Information显示文摘The Roosevelt Hot Springs Known Geothermal Resource Area(KGRA) is a Basin and Range-type geothermal resource, which is located in southwestern Utah. The integrated multicomponent geothermometry(IMG) approach is used to estimate the reservoir temperature at the Roosevelt Hot Springs KGRA. Geothermometric modeling results indicate the deep reservoir temperature is approximately 284.6°C. A conceptual model of the Roosevelt Hot Spring KGRA is provided through integrating the various pieces of exploration information, including the geological data, geothermometric results, temperature well log and field evidence. A two-dimensional cross-sectional model was thus built to quantitatively investigate the coupled thermal-hydraulic processes in the Roosevelt geothermal field. By matching the preproduction temperature log data of deep wells, parameters controlling flow and heat transport are identified. The method and model presented here may be useful for other geothermal fields with similar conditions. | YU Han XU Tianfu YUAN Yilong FENG Guanhong | 2021 | Acta Geologica Sinica(English Edition)2021，95，6： | 0 |
| 11 | GmNMHC5 may promote nodulation via interaction with GmGAI in soybean显示文摘Soybean(Glycine max[L.]Merr.)is a food and oil crop whose growth and yield are influenced by root and nodule development.In the present study,Gm NMHC5 was found to promote the formation of nodules in overexpressing mutants.In contrast,the number of nodules in Gmnmhc5 edited with CRISPR/Cas9 decreased sharply.In 35 S:Gm NMHC5 mutants,expression levels of genes involved in nodulation were significantly up-regulated.Both in vitro and in vivo biochemical analyses showed that Gm NMHC5 directly interacted with Gm GAI(a DELLA protein),and the content of gibberellin 3(GA_(3))in overexpressing mutants was lower than that in the wild type.These results revealed that Gm NMHC5 participates in the classical GA signaling pathway,and may regulate the content of GA_3 to match the optimal concentration required for nodule formation,thereby promoting nodulation by directly interacting with Gm GAI.A model illustrating the mechanism by which Gm NMHC5 promotes soybean nodulation is presented. | Wenting Wang Zhili Wang Wensheng Hou Li Chen Bingjun Jiang Wenya Ma Lijuan Bai Wenwen Song Cailong Xu Tianfu Han Yongjun Feng Cunxiang Wu | 2022 | The Crop Journal2022，10，1： | 0 |
| 12 | A telomere-to-telomere genome assembly of Zhonghuang 13,a widely-grown soybean variety from the original center of Glycine max显示文摘Soybean(Glycine max)stands as a globally significant agricultural crop,and the comprehensive assembly of its genome is of paramount importance for unraveling its biological characteristics and evolutionary history.Nevertheless,previous soybean genome assemblies have harbored gaps and incompleteness,which have constrained in-depth investigations into soybean.Here,we present Telomere-to-Telomere(T2T)assembly of the Chinese soybean cultivar Zhonghuang 13(ZH13)genome,termed ZH13-T2T,utilizing PacBio Hifi and ONT ultralong reads.We employed a multi-assembler approach,integrating Hifiasm,NextDenovo,and Canu,to minimize biases and enhance assembly accuracy.The assembly spans 1,015,024,879 bp,effectively resolving all 393 gaps that previously plagued the reference genome.Our annotation efforts identified 50,564 high-confidence protein-coding genes,707 of which are novel.ZH13-T2T revealed longer chromosomes,421 not-aligned regions(NARs),112 structure variations(SVs),and a substantial expansion of repetitive element compared to earlier assemblies.Specifically,we identified 25.67 Mb of tandem repeats,an enrichment of 5S and 48S rDNAs,and characterized their genotypic diversity.In summary,we deliver the first complete Chinese soybean cultivar T2T genome.The comprehensive annotation,along with precise centromere and telomere characterization,as well as insights into structural variations,further enhance our understanding of soybean genetics and evolution. | Anqi Zhang Tangchao Kong Baiquan Sun Shizheng Qiu Jiahe Guo Shuyong Ruan Yu Guo Jirui Guo Zhishuai Zhang Yue Liu Zheng Hu Tao Jiang Yadong Liu Shuqi Cao Shi Sun Tingting Wu Huilong Hong Bingjun Jiang Maoxiang Yang Xiangyu Yao Yang Hu Bo Liu Tianfu Han Yadong Wang | 2024 | The Crop Journal2024，12，1： | 0 |
| 13 | Analysis of Microstructure of Reactor Pressure Vessel Steel due to Thermal Aging Effect by Small-angle Neutron Scattering显示文摘Reactor pressure vessels are of great importance for nuclear power safety and play an extremely important role in the safe operation of reactors and in preventing nuclear leakage.As material microstructure probe,neutrons have the advantages of high penetrability,ability to distinguish between adjacent nuclide and sensitivity to magnetism.The small-angle neutron scattering can accurately obtain the size distribution,volume fraction and composition of the defects of the nanosized copper clusters in the pressure vessel steel matrix,revealing the microstructure mechanism of the macroscopic mechanical properties.The thermal aging supervision samples from a nuclear power plant in China had been analyzed by small-angle neutron scattering. | LI Bonan WANG Zijun LIU Rongdeng HAN Songbai SUN Kai CHEN Dongfeng LIU Yuntao WANG Chenglong TONG Zhenfeng LI Tianfu | 2018 | 中国原子能科学研究院年报2018，，1： | 0 |
| 14 | Identification of transgene insertions in two genetically modified soybeans using high throughput next generation sequencing显示文摘Genetically modified(GM) organisms are widely adopted. However, their safety assessments and control are still of special concern to the public. Identifying and localizing transgene insertion is an essentially prerequisite step. In this study, 2 independent transgene soybean lines were selected(LB4-AtDCGS-1-20-5-2 and CGS-ZG11) as typical cases. Both lines contained expression cassette of At-DCGS that encoding a feedback-insensitive cystathionine gamma-synthase to produce higher level methionine(Met). LB4-AtDCGS-1-20-5-2 was whole genome sequenced with one paired-end 500 bp library and two mate-paired 1 kb and 2 kb libraries using Illumina HiSeq sequencing platform. CGS-ZG11 was sequenced with only one paired-end 500 bp library. Both genomes were assembled,and 2 scaffold sequences(1 for each line) were screened out by aligning with transgene.Then the transgene insertion and its flanking regions in soybean genome were further identified and confirmed by PCR cloning and Sanger sequencing. Results showed that these 2 transgene lines had single copy of inserted transgene. Their transgene insertion contents were identified, which facilitates further safety assessment. These results indicated that genome assembly using high throughput sequencing is a powerful tool for identifying transgene insertions, even with limited knowledge. | Shouwei Zhang Shi Sun Jing Sun Xiujie Liu Wensheng Hou Tingting Wu Cunxiang Wu Tianfu Han Bingjun Jiang | 2018 | Oil Crop Science2018，3，2： | 0 |
| 15 | 长日照对光周期敏感型大豆顶生花序发育的影响显示文摘顶生花序是识别大豆类型的重要组分,在本研究中,选用晚熟且对光周期敏感的大豆,研究长日照对此大豆顶生花序形成和发育的影响。出苗后,大豆幼苗经12h短日照,光周期13d,以便诱导顶生花序的开始。然后, | Yan Jiang Cunxiang Wu Lingxiao Zhang Po Hu Wensheng Hou Wei Zu Tianfu Han 尚占江 | 2012 | 大豆科技2012，，1： | 0 |
| 16 | Elevated methionine content in soybean seed by overexpressing maizeβ-zein protein显示文摘Soybean provides superior and readily available protein for human and livestock.However,nutritional value of soybean is limited due to the deficiency of an essential amino acid,methionine.To improve total methionine content of soybean,a methionine-rich seed storage protein,β-zein,was introduced into soybean cultivar’Jack’under the control of legumin B4 promoter or Ca MV 35S promoter.Totally 4 T3transgenic lines exhibited higher expression levels of foreign genes,and legumin B4 promoter directed a stronger accumulation ofβ-zein protein than Ca MV 35S promoter.Compared to wild type plant,total methionine content in transgenic soybean seeds significantly increased by up to approximately 15%.Although the introduction ofβ-zein gene improved total methionine content,the level was negligible compared to native soybean storage proteins,implying that the inadequate soluble methionine is the limiting factor.Based on these observations,a new strategy for simultaneously increasing the'source'and'sink'of methionine metabolism is proposed to further improvement of total methionine content in soybean seed. | Chen Guo Xiujie Liu Li Chen Yupeng Cai Weiwei Yao Shan Yuan Cunxiang Wu Tianfu Han Shi Sun Wensheng Hou | 2020 | Oil Crop Science2020，5，1： | 0 |
| 17 | Collection and analysis of stem exudate from soybean seedling显示文摘Vascular tissue serves as the channel for nutrient transport and signal transduction between different organs in plants. To study molecular identity and behavior of mobile substances transmitted between organs via vascular tissue, it is necessary to collect exudate from stem or other organs. Modified stem-cutting method for exudate collection in soybean was used in this study by selecting the optimum sampling time and position, using reagents preventing RNA degradation, etc. Diurnal dynamics analysis of exudate emission was found to be the highest during 10:00-10:10 am. Totally 15 μL pure exudate was collected from the stem cut between cotyledonary and unifoliolate nodes at V1 stage(unifoliolate just expanded) of young soybean seedling. Improved TRIzol method was used to extract RNA and protein from stem exudate. A phloem specific gene of Glycine max sieve element occlusion s, SEO, in exudate samples was successfully amplified by RT-PCR, which comfirmed the success of RNA extraction. SDS-PAGE showed the majority of proteins in exudate were of low molecular weight. Method proposed in this study would facilitate collection of quality exudate and enhance further investigation of mobile substances in soybean. | Xin Xu Zhijian Chen Yanping Qi Lixin Zhang Enoch Sapey Wei Liu Shi Sun Wensheng Hou Tianfu Han | 2018 | Oil Crop Science2018，3，3： | 0 |
| 18 | Small Angle Scattering Study of PAMAM Dendrimer and Its Complex显示文摘The temperature effect on the intermolecular structure of generation 5(G5)poly(amidoamine)(PAMAM)dendrimer in aqueous solutions at different acidic conditions were investigated by using small angle neutron and X-ray scattering(SAS)technique.Upon increasing temperature,a dramatic broadening of the intermolecular structure factor S(Q)peak in the SAS results is clearly shown in Fig.1.This can be attributed | LI Tianfu HAN Wenze WANG Yu CHEN Dongfeng LIU Yuntao LIU Rongdeng ZHANG Li WANG Zijun LI Bonan | 2017 | Annual Report of China Institute of Atomic Energy2017，，1： | 0 |
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