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| 1 | Proteasomal deubiquitinase UCH37 inhibits degradation of β-catenin and promotes cell proliferation and motility显示文摘The ubiquitin–proteasome system degrades most cellular proteins in eukaryotes.UCH37,also known as UCH-L5,is a deubiquitinase binding to Rpn13,a receptor for ubiquitinated substrates in the 26 S proteasome.But,it remains unclear how UCH37 influences the proteasomal degradation of the ubiquitinated substrates.Because deletion of UCH37 is embryonically lethal in mice,this study aims to investigate the role of UCH37 in proteasomal degradation by constructing the UCH37-deficient cell lines using CRISPR/Cas9 technology.Our results demonstrated that deletion of UCH37 decreased the levels of proteasomal Rpn13,implying that UCH37 might facilitate incorporation of Rpn13 into the proteasome.Meanwhile,deletion of UCH37 decreased the levels of β-catenin and the early endosomal protein Rab8.β-Catenin interacts with TCF/LEF to control transcription,and is involved in development,tissue homeostasis and tumorigenesis.We further found that deletion of UCH37 increased the levels of the ubiquitinated β-catenin and accelerated the hydrogen peroxide-stimulated degradation of β-catenin.Deletion of UCH37 also down-regulated the transcription of c-Myc,a downstream effector of β-catenin,and inhibited cell proliferation and motility.These results raise the possibility that UCH37 maintains the homeostasis of proteasomal degradation reciprocally by assisting the recruitment of the ubiquitin receptor Rpn13 into the proteasome and by reversing ubiquitination of certain critical substrates of the 26 S proteasome. | Zijian Li Luming Zhou Tianxia Jiang Libin Fan Xiaoying Liu Xiaobo Qiu | 2019 | Acta Biochimica et Biophysica Sinica2019，51，3： | 4 |
| 2 | Study of Periodical Message-Scheduling AL Algorithm Without Collision of CAN Bus显示文摘 | Cao Wanke Zhang Tianxia Liu Yingji | 2006 | Natural Science Research2006，8，6： | 1 |
| 3 | Crosstalk between CYP2E1 and PPARα substrates and agonists modulate adipose browning and obesity显示文摘Although the functions of metabolic enzymes and nuclear receptors in controlling physiological homeostasis have been established, their crosstalk in modulating metabolic disease has not been explored.Genetic ablation of the xenobiotic-metabolizing cytochrome P450 enzyme CYP2 E1 in mice markedly induced adipose browning and increased energy expenditure to improve obesity. CYP2 E1 deficiency activated the expression of hepatic peroxisome proliferator-activated receptor alpha(PPARa) target genes,including fibroblast growth factor(FGF) 21, that upon release from the liver, enhanced adipose browning and energy expenditure to decrease obesity. Nineteen metabolites were increased in Cyp2 e1-null mice as revealed by global untargeted metabolomics, among which four compounds, lysophosphatidylcholine and three polyunsaturated fatty acids were found to be directly metabolized by CYP2 E1 and to serve as PPARa agonists, thus explaining how CYP2 E1 deficiency causes hepatic PPARa activation through increasing cellular levels of endogenous PPARa agonists. Translationally, a CYP2 E1 inhibitor was found to activate the PPARa-FGF21-beige adipose axis and decrease obesity in wild-type mice, but not in liver-specific Pparanull mice. The present results establish a metabolic crosstalk between PPARa and CYP2 E1 that supports the potential for a novel anti-obesity strategy of activating adipose tissue browning by targeting the CYP2 E1 to modulate endogenous metabolites beyond its canonical role in xenobiotic-metabolism. | Youbo Zhang Tingting Yan Tianxia Wang Xiaoyan Liu Keisuke Hamada Dongxue Sun Yizheng Sun Yanfang Yang Jing Wang Shogo Takahashi Qiong Wang Kristopher W.Krausz Changtao Jiang Cen Xie Xiuwei Yang Frank J.Gonzalez | 2022 | Acta Pharmaceutica Sinica B2022，12，5： | 1 |
| 4 | Role of TiF 3 catalyst in the tribological properties of biofuel soot-contaminated liquid paraffin显示文摘 | Enzhu Hu Xianguo Hu Tianxia Liu Ruhong Song Karl D Dearn Hongming Xu | 2014 | Tribology International2014，，： | 1 |
| 5 | Investigation of morphology, structure and composition of biomass-oil soot particles 显示文摘 | Hu Enzhu Flu Xianguo Liu Tianxia Liu Yiming Song Ruhong Chen Yazhou | 2013 | Applied Surface Science2013，270，： | 1 |
| 6 | The role of soot particles in the tribologieal behavior of engine lubricating oils 显示文摘 | Hu Enzhu Hu Xianguo Liu Tianxia Fang Ling Karl D D Xu Hongming | 2013 | Wear2013，304，： | 1 |
| 7 | Formation of thermogenic adipocytes: What we have learned from pigs显示文摘Thermogenic adipocytes, including brown and beige adipocytes, are capable of dissipating energy via heat production in response to external stimuli, such as cold exposure and exercise. This distinct feature makes them promising targets for therapy of obesity and related metabolic disease. In most mammals, the thermogenic function relies on uncoupling protein 1 (UCP1), which creates a proton leak across the inner mitochondrial membrane, diverting protons away from ATP synthesis and resulting in heat dissipation. However, that is not the case in pigs. Accumulating evidence suggested that in pigs neither functional UCP1 nor brown adipocytes were identified, which might be the leading cause for cold stress-caused neonatal mortality and greater adiposity. In our previous study, beige adipocyte formation was found in cold-tolerant pig breeds, together with the finding that UCP1 knock-in improved thermoregulatory ability and decreased fat deposition in pigs, suggesting the potential application of beige induction in pigs for both agricultural purpose and biomedical implication. In this context, we review the anatomical features, developmental origins, plasticity, transcriptional regulation, and the metabolic benefits of thermogenic adipocytes. Particularly, we describe the contribution of beige adipocytes and reconstitution of UCP1 to improve cold sensitivity and decrease fat deposition in pigs. Furthermore, future undertaking regarding beige adipocyte formation in pigs and its potential application is also discussed. | Jianguo Zhao Cong Tao Chuanhe Chen Yanfang Wang Tianxia Liu | 2021 | Fundamental Research2021，1，4： | 0 |
| 8 | Analysis and Suppression of End Flare in AHSS Roll‑Formed Seat Rail显示文摘Roll forming has been widely used to manufacture long channels with complex cross-sections.End flare,one of the typical shape errors,seriously affects the forming accuracy of roll-formed parts,especially using advanced high-strength steel.In this paper,the mechanism of end flare during the roll forming process of a high-strength automobile seat rail is analyzed.The roll forming process of an actual seat rail is designed.The finite element models of the roll forming process and cut-off springback are established to predict the deformation process and occurrence of end flare.Simulation results indicate that the uneven distribution of longitudinal and shear residual stress along the length of the part is the main reason for the end flare.Based on the simulation,two strategies are proposed to mitigate the end flare.Employing multiple bending processes in the transverse direction effectively balances the longitudinal and shear residual stress.Additionally,the longitudinal bending process can make the longitudinal residual stress in the roll-formed parts more homogenised.Finally,verification experiments are carried out,and the forming accuracy of the seat rail is significantly improved. | Tianxia Zou Yang Liu Weiqin Tang Dayong Li | 2023 | Automotive Innovation2023，6，3： | 0 |
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