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| 1 | Live cell imaging and proteomic profiling of endogenous NEAT1 lncRNA by CRISPR/ Cas9-mediated knock-in显示文摘In mammalian cells,long noncoding RNAs(lncRNAs)form complexes with proteins to execute various biological functions such as gene transcription,RNA processing and other signaling activities.However,methods to track endogenous lncRNA dynamics in live cells and screen for lncRNA interacting proteins are limited.Here,we report the development of CERTIS(CRISPR-mediated Endogenous lncRNA Tracking and Immunoprecipitation System)to visualize and isolate endogenous lncRNA,by precisely inserting a 24-repeat MS2 tag into the distal end of lncRNA locus through the CRISPR7Cas9 technology.In this study,we show that CERTIS effectively labeled the paraspeckle lncRNA NEAT1 without disturbing its physiological properties and could monitor the endogenous expression variation of NEAT1.In addition,CERTIS displayed superior performance on both short-and long-term tracking of NEAT1 dynamics in live cells.We found that NEAT1 and paraspeckles were sensitive to topoisomerase I specific inhibitors.Moreover,RNA Immunoprecipitation(RIP)of the MS2-tagged NEAT1 lncRNA successfully revealed several new protein components of paraspeckle.Our results support CERTIS as a tool suitable to track both spatial and temporal lncRNA regulation in live cells as well as study the lncRNA-protein interactomes. | Bohong Chen Shengcheng Deng Tianyu Ge Miaoman Ye Jianping Yu Song Lin Wenbin Ma Zhou Songyang | 2020 | Protein & Cell2020，11，9： | 4 |
| 2 | The New Architecture with Time-Spatial Consistency for 5G Networks显示文摘While operators have started deploying fourth generation(4G) wireless communication systems,which could provide up to1 Gbps downlink peak data rate,the improved system capacity is still insufficient to meet the drastically increasing demand of mobile users over the next decade.The main causes of the above-mentioned phenomenon include the following two aspects:1) the growth rate of the network capacity is far below that of user's demand,and 2) the relatively deterministic wireless access network(WAN) architecture in the existing systems cannot accommodate the prominent increase of mobile traffic with space-time domain dynamics.In order to address the above-mentioned challenges,we investigate the time-spatial consistency architecture for the future WAN,whilst emphasizing the critical roles of some spectral-efficient techniques such as Massive multiple-input multiple-output(MIMO),full-duplex(FD)operation and heterogeneous networks(HetNets).Furthermore,the energy efficiency(EE)of the HetNets under the proposed architecture is also evaluated,showing that the proposed user-selected uplink power control algorithm outperforms the traditional stochastic-scheduling strategy in terms of both capacity and EE in a two-tier HetNet.The other critical issues,including the tidal effect,the temporal failure owing to the instantaneously increased traffic,and the network wide load-balancing problem,etc.,are also anticipated to be addressed in the proposed architecture.(Abstract) | SUN Zhennian XU Xu CHAI Xiaomeng ZHANG Tianyu TIAN Lin ZHANG Zhongshan | 2016 | China Communications2016，13，1： | 3 |
| 3 | Robotic single-incision left hemihepatectomy for intrahepatic bile duct stones by Da Vinci single-site surgical system: A case report with video显示文摘Minimally invasive surgery is a trend in hepatobiliary surgery.A 56-year-old female patient was admitted to our institution for intrahepatic lithiasis.The CT scan showed multiple calculi in the left liver,dilation of the left intrahepatic bile duct and liver atrophy of the left lobe.Robotic single-incision left hemihepatectomy by the single-site systemwas successfully applied.With the idea of enhanced recovery after surgery,the patient was discharged on the third day after the operation without any morbidity.Robotic single-incision surgery is more frequent in gynecologic and urological surgery.As far as we know,this is the first robotic single-incision left hemihepatectomy report in the world. | Xiao Liang Junhao Zheng Jiaqi Gao Yubin Sheng Tianyu Lin Xiujun Cai | 2021 | Laparoscopic, Endoscopic and Robotic Surgery2021，4，3： | 3 |
| 4 | Hippo-YAP/TAZ signaling in osteogenesis and macrophage polarization:Therapeutic implications in bone defect repair显示文摘Bone defects caused by diseases or surgery are a common clinical problem.Researchers are devoted to finding biological mechanisms that accelerate bone defect repair,which is a complex and continuous process controlled by many factors.As members of transcriptional costimulatory molecules,Yes-associated protein(YAP)and transcriptional co-activator with PDZ-binding motif(TAZ)play an important regulatory role in osteogenesis,and they affect cell function by regulating the expression of osteogenic genes in osteogenesis-related cells.Macrophages are an important group of cells whose function is regulated by YAP/TAZ.Currently,the relationship between YAP/TAZ and macrophage polarization has attracted increasing attention.In bone tissue,YAP/TAZ can realize diverse osteogenic regulation by mediating macrophage polarization.Macrophages polarize into M1 and M2 phenotypes under different stimuli.M1 macrophages dominate the inflammatory response by releasing a number of inflammatory mediators in the early phase of bone defect repair,while massive aggregation of M2 macrophages is beneficial for inflammation resolution and tissue repair,as they secrete many anti-inflammatory and osteogenesis-related cytokines.The mechanism of YAP/TAZ-mediated macrophage polarization during osteogenesis warrants further study and it is likely to be a promising strategy for bone defect repair.In this article,we review the effect of Hippo-YAP/TAZ signaling and macrophage polarization on bone defect repair,and highlight the regulation of macrophage polarization by YAP/TAZ. | Haochen Wang Hui Yu Tianyu Huang Bin Wang Lin Xiang | 2023 | Genes & Diseases2023，10，6： | 2 |
| 5 | High-gain integrated in-line few-mode amplifier enabling 3840-km long-haul transmission显示文摘An integrated few-mode erbium-doped fiber amplifier(FM-EDFA)with high modal gain is suitable for the in-line amplification in mode-division multiplexing transmission(MDM)systems.We first experimentally demonstrate a dual-stage integrated FM-EDFA supporting three linear polarization modes.Consisting of integrated passive components with low insertion losses,the FM-EDFA has a similar structure and performance to widely used commercial single-mode EDFAs.The averaged modal gain of 25 dB,the differential modal gain(DMG)of<1.1 dB,and noise figures of 5–7 dB are simultaneously achieved.In addition,the DMG of the 3M-EDF itself is~0.3 dB.Moreover,an MDM transmission experiment with the in-line few-mode amplification by our proposed FM-EDFA over a 3840-km few-mode fiber link for a 28-Gbaud quadrature phase-shift keying(QPSK)signal is demonstrated. | TAo Xu TIANYU GAO YANZE WANG WENHAO LI WEI LI CHENG Du ZHIQUN YANG YAPING LIU LIN ZHANG | 2022 | Photonics Research2022，10，12： | 2 |
| 6 | Consumption function and its measurement based on the income distribution显示文摘 | Yang Tianyu Lin Gang | 2000 | Consumer Economy2000，，3： | 1 |
| 7 | A Modified Suture Technique for Transperitoneal Laparoscopic Dismembered Pyeloplasty of Pelvi-ureteric Junction Obstruction显示文摘 | Kunlin Yang Lin Yao Xuesong Li Cuijian Zhang Tianyu Wang Lei Zhang Dong Fang Zhisong He Liqun Zhou | 2014 | Urology2014，，： | 1 |
| 8 | The Current Situation and Development of Grain Storage Technologies and Facilities for Chinese Farmers显示文摘 | Fujun Li Tianyu Shi Yang Cao Yi Wu Lin Tian | 2016 | Journal of Food Science and Engineering2016，6，5： | 1 |
| 9 | A high-speed 2D optoelectronic in-memory computing device with 6-bit storage and pattern recognition capabilities显示文摘The explosively developed era of big-data compels the increasing demand of nonvolatile memory with high efficiency and excellent storage properties.Herein,we fabricated a high-speed photoelectric multilevel memory device for neuromorphic computing.The novel two-dimensional(2D)MoSSe with a unique Janus structure was employed as the channel,and the stack of Al_(2)O_(3)/black phosphorus quantum dots(BPQDs)/Al_(2)O_(3)was adopted as the dielectric.The storage performance of the resulting memory could be verified by the endurance and retention tests,in which the device could remain stable states of programming and erasing even after 1,000 cycles and 1,000 s.The multibit storage could be realized through both different voltage amplitudes and pulse numbers,which could achieve 6 bits(64 distinguishable levels)under pulse width of 50 ns.Furthermore,our memory device also could realize the simulations of synapses in human brain with optical and electric modulations synergistically,such as excitatory post-synaptic current(EPSC),long-term potentiation/depression(LTP/LTD),and spike-timing-dependent plasticity(STDP).Neuromorphic computing was successfully achieved through a high recognition of handwritten digits up to 92.5%after 103 epochs.This research is a promising avenue for the future development of efficient memory and artificial neural network systems. | Jialin Meng Tianyu Wang Zhenyu He Qingxuan Li Hao Zhu Li Ji Lin Chen Qingqing Sun David Wei Zhang | 2022 | Nano Research2022，15，3： | 1 |
| 10 | Prediction and controlling for welding deformation of propeller base structure显示文摘Welding deformation often brings about manufacturing problems such as dimensional inaccuracies during assembly and reduces fabrication efficiency.Prediction and controlling welding deformation can help to improve the quality of welded structures.In this paper,the welding deformation of propeller base struc-tures is predicted by means of numerical approaches and mechanical constraints are proposed to control deformations in welding process.Thermal elasto-plastic finite element method(TEP FEM)is employed to simulate welding process of the base structure.Computed accuracy of welding deformation by TEP FEM analysis is verified by comparing with experimental data of tee joint welding.Results show that welding deformations of the base mainly comprise out-of-plane distortion of ring fringe and radial distortion of cylindrical plate.Exerting mechanical constraints of fixed points at fringe and rigid supports inside cylin-der can decrease out-of-plane and radial distortions effectively.The numerical approach adopted in this article can serve as an effective tool to optimize welding process planning in integrated design method. | Junan Yi Jian Lin Zhen Chen Tianyu Chen | 2021 | Journal of Ocean Engineering and Science2021，6，4： | 1 |
| 11 | DDX39B drives colorectal cancer progression by promoting the stability and nuclear translocation of PKM2显示文摘Metastasis is a major cause of colorectal cancer(CRC)mortality,but its molecular mechanisms are still not fully understood.Here,we show that upregulated DDX39B correlates with liver metastases and aggressive phenotypes in CRC.DDX39B is an independent prognostic factor associated with poor clinical outcome in CRC patients.We demonstrate that Sp1 potently activates DDX39B transcription by directly binding to the GC box of the DDX39B promoter in CRC cells.DDX39B overexpression augments the proliferation,migration,and invasion of CRC cells,while the opposite results are obtained in DDX39B-deficient CRC cells.Mechanistically,DDX39B interacts directly with and stabilizes PKM2 by competitively suppressing STUB1-mediated PKM2 ubiquitination and degradation.Importantly,DDX39B recruits importin a5 to accelerate the nuclear translocation of PKM2 independent of ERK1/2-mediated phosphorylation of PKM2,leading to the transactivation of oncogenes and glycolysis-related genes.Consequently,DDX39B enhances glucose uptake and lactate production to activate Warburg effect in CRC.We identify that Arg319 of DDX39B is required for PKM2 binding as well as PKM2 nuclear accumulation and for DDX39B to promote CRC growth and metastasis.In addition,blocking PKM2 nuclear translocation or treatment with glycolytic inhibitor 2-deoxy-D-glucose efficiently abolishes DDX39B-triggered malignant development in CRC.Taken together,ourfindings uncover akey role forDDX39B in modulating glycolytic reprogramming and aggressive progression,and implicate DDX39B as a potential therapeutic target in CRC. | Gang Zhao Hang Yuan Qin Li Jie Zhang Yafei Guo Tianyu Feng Rui Gu Deqiong Ou Siqi Li Kai Li Ping Lin | 2022 | Signal Transduction and Targeted Therapy2022，7，9： | 1 |
| 12 | Constructing data literacy evaluation system for social science scholars显示文摘To establish a data literacy evaluation system for social science scholars is a part of transformation to data-intensive scientific research paradigm in social science.Based on the literature review of data literacy and survey of social science data management features,this paper analyzed the elements of data literacy of social science scholars.The data literacy of social science scholars ma inly consists of data awareness level,data discovery and access ability,data management and organization ability,data processing and analysis ability,data utilization and preservation ability,and data ethics level.Each of these primary indexes has several secondary indexes.It constructed the evaluation system of social science scholars data literacy.The weights of the primary and secondary indexes in the system were calculated by applying AHP.The data literacy evaluation system for social science scholars can provide a reference for assessing and promoting social science scholars'data literacy ability in China. | Lin Wang Xinyu Huang Tianyu Du | 2021 | Data Science and Informetrics2021，1，4： | 1 |
| 13 | 组蛋白H3泛素化通过影响H3K9甲基化进而调控肝脏发育相关基因表达的机制显示文摘文章简介肝脏的发育是一个多步骤并且受到严格调控的过程。过去的研究主要集中在个体出生前肝细胞如何分裂分化,并且多以转录因子和信号通路为研究对象。相比之下,肝脏在个体出生后直至成熟的过程中,虽然肝脏的发育并未停止,但是研究资料相对匮乏。 | Gaofeng Li Tong Ji Jiang Chen Yufei Fu Lidan Hou Yan Feng Tingyue Zhang Tianyu Song Jie Zhao Yoko Endo Hui Lin 蔡秀军 仓勇 | 2018 | 科学新闻2018，0，4： | 0 |
| 14 | Improved multiferroic in EuTiO_(3) films by interphase strain engineering显示文摘Interphase strain engineering provides a unique methodology to significantly modify the lattice structure across a single film,enabling the emergence and manipulation of novel functionalities that are inaccessible in the context of traditional strain engineering methods.In this work,by using the interphase strain,we achieve a ferromagnetic state with enhanced Curie temperature and a room-temperature polar state in EuO secondary phase-tunned EuTiO_(3) thin films.A combination of atomic-scale electron microscopy and synchrotron X-ray spectroscopy unravels the underlying mechanisms of the ferroelectric and ferromagnetic properties enhancement.Wherein,the EuO secondary phase is found to be able to dramatically distort the TiO_6 octahedra,which favors the non-centrosymmetric polar state,weakens antiferromagnetic Eu-Ti-Eu interactions,and enhances ferromagnetic Eu-O-Eu interactions.Our work demonstrates the feasibility and effectiveness of interphase strain engineering in simultaneously promoting ferroelectric and ferromagnetic performance,which would provide new thinking on the property regulation of numerous strongly correlated functional materials. | Yiyan Fan Shiqing Q.Deng Tianyu Li Qinghua Zhang Shuai Xu Hao Li Chuanrui Huo jiaou Wang Lin Gu Kuijuan Jin Oswaldo Dieguez Er-jia Guo Jun Chen | 2023 | Chinese Chemical Letters2023，34，7： | 0 |
| 15 | Data Analyses and Parallel Optimization of the Regional Marine Ecological Model显示文摘Under the joint influence of high-intensity human activities and cli-mate change,the coastal ecological environment is deteriorating,and the ecologi-cal environment security and the sustainable development of the marine economy are seriously threatened.Therefore,it is of great significance to establish a high-resolution ecological environment operational forecasting system.To meet the run time requirements of the ecological operational forecasting system,a vari-ety of parallel optimization methods were proposed to improve the operation efficiency of the model.First,based on the National Marine Environmental Fore-casting Center’s Lenovo cluster,the ROMS benchmark experiment was expanded to the 4000 Processes scale.A good speedup was obtained by the experiment.The ROMS model was analysed with strong scalability.Second,in the hydrodynamic-ecological simulation experiment of the Bohai Sea-Yellow Sea-East China Sea,by optimizing Vector,InfiniBand,and Parallel I/O,the performance of the model can be improved by 270%while maintaining the same computing resources.That computing resources were more reasonably used lay the foundation for the operational forecast. | Yanqiang Wang Jingjing Zheng Tianyu Zhang Peng Liang Bo Lin | 2023 | 国际计算机前沿大会会议论文集2023，，2： | 0 |
| 16 | LDL receptor-related protein 1 (LRP1),a novel target for opening the blood-labyrinth barrier(BLB)显示文摘Inner ear disorders are a cluster of diseases that cause hearing loss in more than 1.5 billion people worldwide.However,the presence of the blood-labyrinth barrier(BLB)on the surface of the inner ear capillaries greatly hinders the effectiveness of systemic drugs for prevention and intervention due to the low permeability,which restricts the entry of most drug compounds from the bloodstream into the inner ear tissue.Here,we report the finding of a novel receptor,low-density lipoprotein receptor-related protein 1(LRP1),that is expressed on the BLB,as a potential target for shuttling therapeutics across this barrier.As a proof-ofconcept,we developed an LRP1-binding peptide,IETP2,and covalently conjugated a series of model small-molecule compounds to it,including potential drugs and imaging agents.All compounds were successfully delivered into the inner ear and inner ear lymph,indicating that targeting the receptor LRP1 is a promising strategy to enhance the permeability of the BLB.The discovery of the receptor LRP1 will illuminate developing strategies for crossing the BLB and for improving systemic drug delivery for inner ear disorders. | Xi Shi Zihao Wang Wei Ren Long Chen Cong Xu Menghua Li Shiyong Fan Yuru Xu Mengbing Chen Fanjun Zheng Wenyuan Zhang Xinbo Zhou Yue Zhang Shiwei Qiu Liyuan Wu Peng Zhou Xinze Lv Tianyu Cui Yuehua Qiao Hui Zhao Weiwei Guo Wei Chen Song Li Wu Zhong Jian Lin Shiming Yang | 2022 | Signal Transduction and Targeted Therapy2022，7，7： | 0 |
| 17 | Targeting LncRNA LLNLR-299G3.1 with antisense oligonucleotide inhibits malignancy of esophageal squamous cell carcinoma cells in vitro and in vivo显示文摘Accumulating evidence has indicated that long non-coding RNAs(lncRNAs)play critical roles in the development and progression of cancers,including esophageal squamous cell carcinoma(ESCC).However,the mechanisms of lncRNAs in ESCC are still incompletely understood and therapeutic attempts for in vivo targeting cancer-associated lncRNA remain a challenge.By RNA-sequencing analysis,we identified that LLNLR-299G3.1 was a novel ESCC-associated lncRNA.LLNLR-299G3.1 was up-regulated in ESCC tissues and cells and promoted ESCC cell proliferation and invasion.Silencing of LLNLR-299G3.1 with ASO(antisense oligonucleotide)resulted in opposite effects.Mechanistically,LLNLR-299G3.1 bound to cancerassociated RNA binding proteins and regulated the expression of cancer-related genes,including OSM,TNFRSF4,HRH3,and SSTR3.ChIRP-seq(chromatin isolation by RNA purification and sequencing)revealed that these genes contained enriched chromatin binding sites for LLNLR-299G3.1.Rescue experiments confirmed that the effects of LLNLR-299G3.1 on ESCC cell proliferation were dependent on interaction with HRH3 and TNFRSF4.Therapeutically,intravenous delivery of placental chondroitin sulfate A binding peptide-coated nanoparticles containing antisense oligonucleotide(pICSA-BP-ANPs)strongly inhibited ESCC tumor growth and significantly improved animal survival in vivo.Overall,our results suggest that LLNLR-299G3.1 promotes ESCC malignancy through regulating gene-chromatin interactions and targeting ESCC by pICSA-BP-ANPs may be an effective strategy for the treatment of lncRNA-associated ESCC. | LI TIAN YONGYI HUANG BAOZHEN ZHANG YI SONG LIN YANG QIANQIAN CHEN ZHENG WANG YILING WANG QIHAN HE WENHAN YANG SHUYONG YU TIANYU LU ZICHEN LIU KAIPING GAO XIUJUN FAN JIAN SONG RIHONG ZHAI | 2023 | Oncology Research2023，31，4： | 0 |
| 18 | Data Analyses and Parallel Optimization of the Tropical-Cyclone Coupled Numerical Model显示文摘Tropical cyclones(TCs)are one of the most feared and deadly weather systems in the world.An air-sea coupled numerical model offers a more accurate description of physical processes between atmospheric-ocean fluids.An operational ocean-atmosphere-wave coupled modeling system is employed to improve the prediction accuracy of tropical cyclones in the NationalMarine Environmental Forecasting Center(NMEFC).Due to the urgent need for operational timeliness,the parallel performance of the operational forecasting system has been analyzed.The parallel algorithm,parallel partitioning grids,and other optimizations were tested after system deployment on the Lenovo cluster of the NMEFC.After optimization,a well-balanced performance of the system is obtained,and computing resources are reasonably utilized,thus laying the foundation for real-time tropical cyclone forecasting. | Yanqiang Wang Tianyu Zhang Zhaohui Yin Sai Hao Chenqi Wang Bo Lin | 2022 | 国际计算机前沿大会会议论文集2022，，1： | 0 |
| 19 | Investigating the reason for high FF from ternary organic solar cells显示文摘Organic solar cells(OSCs)have attracted huge attention because of their unique merits[1−3].In last few years,thanks to the design of new materials and device engineering,the power conversion efficiencies(PCEs)of OSCs have surpassed 18%[4−8].The PM6:Y6 cells are efficient binary cells,offering high PCEs over 16%[9−11].The high performance originates from the efficient free charge generation and the ground state dipole field at the donor-acceptor interface that promotes the exciton dissociation[12]. | Danqin Li Yihan Zeng Zeng Chen Shifeng Leng Zuo Xiao Qifan Xue Tianyu Hao Meng Lv Hongbo Wu Lina Lin Jianming Yang Zaifei Ma Jinquan Chen Rong Huang Feng Liu Haiming Zhu Xianjie Liu Liming Ding Mats Fahlman Qinye Bao | 2021 | Journal of Semiconductors2021，42，9： | 0 |
| 20 | Remote Detection of Hydrocarbon Microseepage in a Loess Covered Area显示文摘Hydrocarbon microseepage can result in related near-surface mineral alterations.In this study,we evaluated the potential of detecting these alterations with field measured and satellite acquired hyperspectral data.Fourteen soil samples and reflectance spectra were collected in the Xifeng Oilfield,a loess covered area.Soil samples were analyzed in the laboratory for calcite,dolomite,kaolinite,illite,and mixedlayer illite/smectite content,and we processed reflectance spectra for continuum removal to derive clay and carbonate mineral absorption depth(H).High correlation between absorption depth and mineral content was shown for clay and mineral carbonate with field measured spectra.Based on the result for the field spectra,we proposed and tested a fast index based on the absorption depth of clay and carbonate minerals with a hyperspectral image of the area.The detected hydrocarbon microseepage anomalies matched well with those shown in the geological map. | Liang Zhao Darning Wang Shengbo Chen Lin Li Tianyu Zhang | 2020 | Journal of Earth Science2020，31，1： | 0 |
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