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| 1 | A sensitive DNA electrochemical biosensor based on magnetite with a glassy carbon electrode modified by muti-walled carbon nanotubes in polypyrrole 显示文摘 | Guifang Cheng Jie Zhao Yonghua Tu | 2005 | Analytica Chimica Acta2005，533，： | 1 |
| 2 | Effects of extracts from soothing-liver and invigorating-spleen formulas on the injury induced by oxidative stress in the hepatocytes of rats with non-alcoholic fatty liver disease induced by high-fat diet显示文摘OBJECTIVE:To investigate effects of the extracts from soothing-liver and invigorating-spleen formulas on the injury due to oxidative stress,mediated by the Nuclear factor-like 2(Nrf2)-Antioxidant response element(ARE) pathway,in the hepatocytes of rats with non-alcoholic fatty liver disease(NAFLD) induced by high-fat diet.METHODS:Soothing-liver and invigorating-spleen formula mixtures were prepared for five groups:normal,model,soothing-liver formula group(SLG),invigorating-spleen formula group(ISG),integrated formula group(IG).The rat model of NAFLD was induced by feeding rats a high-fat diet(HFD).After 16 weeks,the hepatic tissue was examined following Haematoxylin-Eosin(H&E) staining and with Transmission electron microscopy(TEM).Levels of hepatic lipids,serum lipids and hepatic functions were measured using a biochemical analyser.Hepatocytes were isolated from the livers of rats and were identified by cellar immunohistochemistry,cellular immunofluorescence and flow cytometry.The expression levels of Nrf2,Kelch-like epichlorohydrin-associated protein 1(Keap-1),haeme oxygenase-1(HO-1) and NAD(P)H:quinone oxidoreductase 1(NQO1) m RNAs were assessed by real-time fluorescence quantitative PCR.Nrf2,Keap-1,HO-1 and NQO1 proteins were measured by Western blotting.Finally,the levels of oxidative stress factors Superoxide Dismutase(SOD),malonaldehyde(MDA) and Glutathione peroxidase(GSH-Px) in hepatocytes were measured by WST-1,TBA and colorimetry.RESULTS:The H & E and TEM results showed that the NAFLD model rats successfully reproduced typical pathogenetic and histopathological features.The liver function and levels of hepatic lipids and serum lipids from the model rats were dramatically increased.Compared with the model group,the levels of hepatic lipids,serum lipids and hepatic function in the treatment groups were ameliorated to different degrees.The yields of purified hepatocytes in each rat were 4-5 × 108.The viability of the isolated hepatocytes was higher than 95%,with a purity over 93.2%.Cellular immunohistochemistry analysis showed that the hepatocytes were brown,while in the cellular immunofluorescence analysis,the hepatocytes showed green fluorescence.The expression levels of Nrf2,Keap-1,HO-1 and NQO1 m RNA and protein in the hepatocytes were significantly higher in the model group than in the normal group(P < 0.05,P < 0.01).Compared with the model group,the expression of Nrf2,Keap-1,HO-1 and NQO-1 m RNAs and proteins in all treatment groups increased,especially in the IG(P < 0.01).CONCLUSION:The extracts from soothing-liver and invigorating-spleen formulas may protect the liver against the injury induced by oxidative stress in hepatocytes by influencing the Nrf2-ARE pathway,which may be the mechanism having the potential for prevention and treatment of NAFLD. | Tu Guifang Gong Xiangwen Yang Qinhe Li Yuanyuan Wang Guanlong Liang Yinji Zhang Yupei Yan Haizhen Lin Chunmei Zhang Jinwen | 2018 | Journal of Traditional Chinese Medicine2018，38，4： | 1 |
| 3 | Effect of Different Concentrations of Lipopolysaccharide on Expression of NF-κB and LAP in Mammary Epithelial Cells of Dairy Cow显示文摘The paper was to study the effects of different concentrations of lipopolysaccharide(LPS) on expression of nuclear factor Kappa B(NF-κB) and lingual antimicrobial peptide(LAP) gene in mammary epithelial cells of dairy cow.The mammary epithelial cells of dairy cow were stimulated by different concentrations(50,100,200,400 and 800 ng/mL) of LPS.The total RNA of cells was extracted after stimulation for 2,4,8,16,24,48 and 72 h,respectively,and the mRNA expression levels of NF-κB P65 and LAP were evaluated by real-time quantitative PCR.The results showed that the expression of NF-κB P65 and LAP mRNA treated with 400 ng / mL LPS for 72 h were the highest compared to the control group(P<0.01).The result confirmed that the expression activity of NF-κB was enhanced in inflammatory effects of mammary epithelial cells induced by LPS,which regulated the expression of defense gene LAP,with certain dose and time effects. | Cheng Lanling Cao Guifang Wen Shiyong Zhao Pengwei Tu Yong Jian Ruizhen Li Qi | 2014 | Animal Husbandry and Feed Science2014，6，1： | 0 |
| 4 | Changes in erythrocyte membrane ATPase and plasma lipid peroxides during perioperative period显示文摘Changes in erythrocyte membrane ATPases and plasma lipid peroxides(LPO) were studiedperioperatively in 15 abdominal surgical patients under intravenous procaine-balanced anesthesia.The resultsrevealed that erythrocyte membrne Na+-K+ATPase,Mg2+ATPase and Ca2+-Mg2+ATPase activities werenot significantly changed at 60~90 min after incision as compared with 30 min before anesthesia,but weredecreased markedly at 10 rain and 24 hours after completion of operation(P<0.01).Plasma LPO wereincreased significantly by 24 hours after surgery(P<0.01) following an initially marked but transientreduction.Plasma LPO changes were not correlated with erythrocyte membrane ATPase activities,r=-0.0396,-0.0097 and-0.4383 respeetively(P>0.05).These correlations are suggested to be inducedby neuroendoerine responses after anesthesia and/or surgical trauma. | Tu Weifeng Lin Guifang Shen Jianfan Department of Anesthesiology,Jinling Hospital,Nanjing,China Department of Anesthesiology,First Affiliated Hospital of Nanjing Medical College,Nanjing,China | 1993 | 医学研究生学报1993，6，S1： | 0 |
| 5 | Mechanisms of Up-regulation of 17β-Estrodiol on β-Defensin-2 ( SBD-2) Expression in Epithelial Cells of Ovine Oviduct显示文摘[Objective]To investigate the mechanisms involved in the Up-regulatory effects of 17β-estrodiol on β-defensin-2( SBD-2) in epithelial cells of ovine oviduct. [Methods]Epithelial cells of ovine oviduct were isolated and cultured; and then the cultured cells at secondary generation were divided into 17β-estradiol( E2,10- 8mol / L) group,estrogen nuclear receptor antagonist ICI182780( 10- 7mol / L) group,PKA antagonist KT-5720( 1 μmol / L) group,PKC antagonist H-7( 50 μmol / L) group,nuclear factor kappa B antagonist PDTC( 50 μmol / L) group and the blank control group( Control). Firstly,different antagonists were added into corresponding antagonist groups in order to interfere the epithelial cells of ovine oviduct for 1 h. Then,17β-estradiol( 10- 8mol / L) was added into each antagonist group and E2 group for cultivation for 6 h. Finally,real-time fluorescent quantitative RT-PCR was used to detect the changes of SBD-2 mRNA expression.[Results]10- 8mol / L 17β-estrodiol had significantly Up-regulatory effects on the expression of SBD-2 mRNA( P < 0. 05). Estrogen nuclear receptor antagonist ICI182780,NF-κΒ antagonist PDTC and PKC antagonist H-7 could all block the Up-regulatory effects on SBD-2. But PKA antagonist KT-5720 showed no significant effects on the Up-regulation of SBD-2 mRNA expression induced by 17β-estrodiol. [Conclusions] SBD-2 mRNA expression induced by 17β-estrodiol in epithelial cells of ovine oviduct was mediated by estrogen nuclear receptor ICI182780,NF-κB and PKC pathways. However,PKA pathway might not participate in the Up-regulation of SBD-2 mRNA expression. | Bao Tuya Cao Guifang Tu Yong Du Chenguang | 2015 | Animal Husbandry and Feed Science2015，7，2： | 0 |
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