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| 1 | Increased Vitamin C Content Accompanied by an Enhanced Recycling Pathway Confers Oxidative Stress Tolerance in Arabidopsis显示文摘Vitamin C(L-ascorbic acid,AsA) has important antioxidant and metabolic functions in both plants and animals.Once used,ascorbic acid can be regenerated from its oxidized form in a reaction catalyzed by dehydroascorbate reductase(DHAR,EC 1.8.5.1).To analyze the physiological role of DHAR catalyzing the reduction of DHA to ascorbate in environmental stress adaptation,we examined whether increasing the level of AsA through enhanced AsA recycling would limit the deleterious effects of oxidative stress.A chimeric construct consisting of the double CaMV35S promoter fused to the Myc-dhar gene was introduced into Arabidopsis thaliana.Transgenic plants were biochemically characterized and tested for responses to oxidative stress.Western blot indicated that the dhar-transgene was successfully expressed.In homozygous T4 transgenic seedlings,DHAR overexpression was increased up to 1.5 to 5.4 fold,which enhanced foliar ascorbic acid levels 2-to 4.25-fold and ratio of AsA/DHA about 3-to 16-fold relative to wild type.In addition,the level of glutathione,the reductant used by DHAR,also increased as did its redox state.When whole plants were treated with high light and high temperature stress or in vitro leaf discs were subjected to 10 μM paraquat,transgenic plants showed a larger AsA pool size,lower membrane damage,and a higher level of chlorophyll compared with controls.These data suggested that increasing the plant vitamin C content through enhanced ascorbate recycling could limit the deleterious effects of environmental oxidative stress. | zinan wang Ying Xiao Wansheng Chen Kexuan Tang Lei Zhang | 2010 | Journal of Integrative Plant Biology2010，52，4： | 16 |
| 2 | Bidirectional propagation of lightning leader显示文摘The characteristics of initial leader of triggered lightning flashes are studied by use of a highspeed digital camera system with the rate of 1000 frame per second and simultaneous measurements of current and electric field change with 0.1 μs time resolution. The results show that the altitude-triggered lightning was initiated by bidirectional propagation leader because of 'current interruption' by air gap,whereas the grounded-triggered one was not. The onset of downward-moving positive leader was prior to that of the upward-going negative leader for 843 ms in an altitude-triggered lightning under the positive ambient electric field. The downward-moving leader, regardless of its polarity, would produce inhomogeneous distribution of luminosity (current) along the channel on the moment of connection to the ground. | Caiwei Wang Muhong Yan Xinsheng Liu Yijun Zhang Wansheng Dong Cuihua Zhang | 1999 | Chinese Science Bulletin1999，44，2： | 8 |
| 3 | MICRO ELECTRICAL DISCHARGE MACHINING DEPOSITION IN AIR显示文摘一个新免职方法用用机器制造的微电的分泌物(EDM ) 被描述在空中在细工品上扔工具电极材料。微电的分泌物免职(EDD ) 的基本原则被分析,认识到的条件被预言。与塑造机器的平常的 EDM,是的黄铜电极,是的高速度的钢细工品,实验的很多系统地并且彻底地在微 EDD 上被执行。处理参数的专业的效果例如分泌物水流,解除持续时间,脉搏间隔和工作媒介,被获得。作为结果,有在直径的 0.19 公里和在高度的 7.35 公里的微柱体被扔。由交换电极和细工品的极性,微柱体能有选择地被移开。免职和移动的可逆用机器制造因此被完成,它突破传统的 EDM 的限制。扔的材料对细工品紧缩、靠近的大小表演基于,其部件取决于工具电极材料。 | JIN Baidong ZHAO Wansheng WANG Zhenlong CAO Guohui | 2006 | Chinese Journal of Mechanical Engineering2006，19，4： | 6 |
| 4 | Genome-wide profiling of RNA editing sites in sheep显示文摘Background: The widely observed RNA-DNA differences(RDDs) have been found to be due to nucleotide alteration by RNA editing. Canonical RNA editing(i.e., A-to-I and C-to-U editing) mediated by the adenosine deaminases acting on RNA(ADAR) family and apolipoprotein B mRNA editing catalytic polypeptide-like(APOBEC)family during the transcriptional process is considered common and essential for the development of an individual.To date, an increasing number of RNA editing sites have been reported in human, rodents, and some farm animals;however, genome-wide detection of RNA editing events in sheep has not been reported. The aim of this study was to identify RNA editing events in sheep by comparing the RNA-seq and DNA-seq data from three biological replicates of the kidney and spleen tissues.Results: A total of 607 and 994 common edited sites within the three biological replicates were identified in the ovine kidney and spleen, respectively. Many of the RDDs were specific to an individual. The RNA editing-related genes identified in the present study might be evolved for specific biological functions in sheep, such as structural constituent of the cytoskeleton and microtubule-based processes. Furthermore, the edited sites found in the ovine BLCAP and NEIL1 genes are in line with those in previous reports on the porcine and human homologs, suggesting the existence of evolutionarily conserved RNA editing sites and they may play an important role in the structure and function of genes.Conclusions: Our study is the first to investigate RNA editing events in sheep. We screened out 607 and 994 RNA editing sites in three biological replicates of the ovine kidney and spleen and annotated 164 and 247 genes in the kidney and spleen, respectively. The gene function and conservation analysis of these RNA editing-related genes suggest that RNA editing is associated with important gene function in sheep. The putative functionally important RNA editing sites reported in the present study will help future studies on the relationship between these edited sites and the genetic traits in sheep. | Yuanyuan Zhang Deping Han Xianggui Dong Jiankui Wang Jianfei Chen Yanzhu Yao Hesham Y.A.Darwish Wansheng Liu Xuemei Deng | 2019 | Journal of Animal Science and Biotechnology2019，10，2： | 2 |
| 5 | A CAD/CAM system for micro - ED - milling of small 3D free- form cavity 显示文摘 | Zhao Wansheng Yang Yang Wang Zhenlong | 2004 | Journal of Materials Processing Technolo- gy2004，149，： | 1 |
| 6 | On the continuity and convexity analysis of the expected value function of a fuzzy mapping 显示文摘 | Wang Cheng Tang Wansheng Zhao Ruiqing | 2007 | Journal of Uncertain Systems2007，1，2： | 1 |
| 7 | A special CAD/ CAM software for electro-discharge machining of shrouded turbine blisks显示文摘 | Li Gang Zhao Wansheng Wang Zhenlong | 2007 | Journal Shanghai University (English Edition)2007，11，1： | 1 |
| 8 | A CAD/CAM system for micro-ED-milling of small 3D freeform cavity显示文摘 | Zhao Wansheng Yang Yang Wang Zhenlong | 2004 | Journal of Materials Processing Technology2004，149，： | 1 |
| 9 | On the numerical solution of nonlinear option pricing equation in illiquid markets显示文摘 | Jianqiang Guo Wansheng Wang | 2014 | Computers and Mathematics with Applications2014，，： | 1 |
| 10 | Facile synthesis of the novel Ag3VO4/AgBr/Ag plasmonic Photocatalyst with enhanced photocatalytic activity and stability显示文摘 | ZHU Qing WANG Wansheng LIN Ling et a1 | 2013 | J Phys Chem C2013，117，11： | 1 |
| 11 | Ultrasonic and electric discharge machining to deep and small hole on titanium alloy 显示文摘 | Zhao Wansheng Wang Zhenlong Di Shichun | 2002 | Jouranl of materials processing technology2002，，120： | 1 |
| 12 | Ultrosonic and electric discharge machining to deep and small hole on titanium alloy显示文摘 | Wansheng Zhao Zhenlong Wang Shichun Di | 2002 | Journal of Materials Processing Technology2002，，120： | 1 |
| 13 | Supplychain coordination by revenue sharing contract with fuzzy demand显示文摘 | WANG Junyan ZHAO Ruiqing TANG Wansheng | 2008 | Journal of Intelligent and Fuzzy Systems2008，19，6： | 1 |
| 14 | Transforming growth factor- and Smad signalling in kidney diseases 显示文摘 | Wang Wansheng Koka Vijay | 2005 | Nephrology2005，10，1： | 1 |
| 15 | A CAD/CAM system for micro-ED-milling of small 3D freeform cavity显示文摘 | ZHAO Wansheng YANG Yang WANG Zhenlong | 2004 | Journal of Materials Processing Technology2004，，149： | 1 |
| 16 | Synthesis and crystal structure of a new supermolecular compound: [C 12 H 24 O 6 ][H 3 PMo 12 O 40 ]·22H 2 O (C 12 H 24 O 6 =18-crown-6)显示文摘 | Wansheng You Enbo Wang Qinglin He Lin Xu Yan Xing Hengqing Jia | 2000 | Journal of Molecular Structure2000，，1： | 1 |
| 17 | Supply chain coordination by revenue-sharing contract with fuzzy demand显示文摘 | WANG Junyan ZHAO Ruiqing TANG Wansheng | 2008 | Journal of Intelligent and Fuzzy Systems2008，19，6： | 1 |
| 18 | Research and Development of Nontraditional Machining in China显示文摘 | ZhuDi Wang Zhenlong Guo Dongming | 2000 | International Journal of Electrical Machining2000，，5： | 1 |
| 19 | Advanced Glycation End Products Induce Tubular Epithelial-Myofibroblast Transition through the RAGE-ERK1/2 MAP Kinase Signaling Pathway显示文摘 | Jin H. Li Wansheng Wang Xiao R. Huang Matthew Oldfield Ann M. Schmidt Mark E. Cooper Hui Y. Lan | 2004 | The American Journal of Pathology2004，，4： | 1 |
| 20 | Ultrasound-Microbubble-Mediated Gene Transfer of Inducible Smad7 Blocks Transforming Growth Factor-β Signaling and Fibrosis in Rat Remnant Kidney显示文摘 | Chun-Cheng Hou Wansheng Wang Xiao R. Huang Ping Fu Tso-Hsiao Chen David Sheikh-Hamad Hui Y. Lan | 2005 | The American Journal of Pathology2005，，3： | 1 |
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