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| 1 | Prediction of permafrost changes in Northeastern China under a changing climate显示文摘Northeastern China has the second largest expanse of permafrost in China,primarily known as Xing'an-Baikal permafrost.Located on the southeastern edges of the Eurasian cryolithozone,the permafrost is thermally unstable and ecologically sensitive to external changes.The combined impacts of climatic,environmental,and anthropogenic changes cause 3-dimensional degradation of the permafrost.To predict these changes on the southern limit and ground temperature of permafrost in Northeastern China,an equivalent latitude model (ELM) for the mean annual ground surface temperature (MAGSTs) was proposed,and further improved to take into account of the influences of vegetation and snow-cover based on observational data and using the SHAW model.Using the finite element method and assuming a climate warming rate of 0.048°C a-1,the ELM was combined with the unsteady-state heat conduction model to simulate permafrost temperatures at present,and to predict those after 50 and 100 a.The results indicate that at present,sporadic permafrost occurs in the zones with MAGSTs of 1.5°C or colder,and there would still be a significant presence of permafrost in the zones with the present MAGSTs of 0.5°C or colder after 50 a,and in those of-0.5°C or colder after 100 a.Furthermore,the total areal extent of permafrost would decrease from 2.57×105 km2 at present to 1.84×105 km2 after 50 a and to 1.29×105 km2 after 100 a,i.e.,a reduction of 28.4% and 49.8% in the permafrost area,respectively.Also the permafrost would degrade more substantially in the east than in the west.Regional warming and thinning of permafrost would also occur.The area of stable permafrost (mean annual ground temperature,or MAGT≤-1.0°C) would decrease from present 1.07×105 to 8.8×104 km2 after 50 a,and further decrease to 5.6×104 km2 after 100 a.As a result,the unstable permafrost and seasonally frozen ground would expand,and the southern limit of permafrost would shift significantly northwards.The changes in the permafrost environment may adversely affect on ecological environments and engineering infrastructures in cold regions.Avoidance of unnecessary anthropogenic changes in permafrost conditions is a practical approach to protect the permafrost environment. | WEI Zhi JIN HuiJun ZHANG JianMing YU ShaoPeng HAN XuJun JI YanJun HE RuiXia CHANG XiaoLi | 2011 | Science China Earth Sciences2011，54，6： | 15 |
| 2 | Process improvement in laser hot wire cladding for martensitic stainless steel based on the Taguchi method显示文摘与低热输入,高精力效率,和高精确的突出的特征,激光热电线 cladding 广泛地被使用因为重新生产的金属分开。然而, cladding 过程需要被使用一个量的方法改善。在这个工作,容量的缺点比率作为标准被建议描述为 cladding 层形成质量的正直。有 FV520B 的激光免职实验, martensitic 不锈钢之一,被使用 Taguchi 方法设计。四个进程变量,也就是激光力量(P) ,扫描速度(V s ), 电线喂率(V f ), 和电线水流(I) ,基于 signal-to-noise (S/N ) 的分析被优化比率。cladding 层的冶金学的观察被进行比较形成的质量并且验证分析方法。有最佳参数联合的稳定、连续的进程与最小的缺点和裂缝生产了一致微观结构,它导致了一个好冶金的结合接口。 | Zilin HUANG Gang WANG Shaopeng WEI Changhong LI Yiming RONG | 2016 | Frontiers of Mechanical Engineering2016，11，3： | 5 |
| 3 | Comparative profiling of the transcriptional response to soybean cyst nematode infection of soybean roots by deep sequencing显示文摘To gain insight into the changes in the transcriptome of soybean roots during soybean cyst nematode (SCN) infection, we conducted genome-wide gene expression profiling using serial analysis of gene expression (SAGE) combined with Solexa sequencing. More than 3 million tags were generated from the SCN-infected and uninfected roots, and 366941 and 314591 clean UniTags were obtained from SCN-infected and uninfected samples, respectively. In the SCN-infected sample, 48249 UniTags represented 18114 reference genes. In the uninfected control, 46290 UniTags represented 19323 reference genes. Comparison of tag frequencies identified 1405 genes that were expressed at greater levels in SCN-infected roots than in uninfected roots, and 1191 genes that were expressed at lower levels. Quantitative real-time PCR analyses confirmed the changes in mRNA levels observed in our sequencing analyses. A comparable number of genes were upand down-regulated in response to nematode infection, indicating that down-regulation of some genes might be essential in the plant response to nematodes. Our SAGE results showed significant changes in expression of many unreported genes involved in nematode infection. Approximately 7% of tags mapped to the antisense strand of genes, indicating widespread antisense transcription. | LI XiaoYan WANG Xue ZHANG ShaoPeng LIU DaWei DUAN YuXi DONG Wei | 2011 | Chinese Science Bulletin2011，56，18： | 5 |
| 4 | Genome research profile of two Cordyceps sinensis cDNA libraries显示文摘Cordyceps sinensis (CS) is well known as an ancient Chinese herb. It is used to expand bronchial smooth muscles, inhibit tumor growth and decrease blood pressure. Cordyceps sinensis is composed of two parts. One is the dead larvae body; the other is the stroma like withered grass. Up to now, few genome database articles about Cordyceps sinensis have been reported. In this study, two cDNA libraries were constructed using the worm part and grass part respectively for the first time. 12192 and 15456 clones from the worm-part (CSCA) and grass-part (CSBA) library were respectively picked. Sequences derived from CSCA were clustered into 1333 contigs and 2469 singlets, while those from CSBA were clustered into 1297 contigs and 2875 singlets. These ESTs include sequences representing a significant portion of proteins encoding genes in cell signalling, metabolism, information storage and processing. Some enzymes encoding genes were also found and linked with CS’s physiology such as proteases, peptidases, lipases and chitinase. Pairwise comparison between the two cDNA libraries was also studied. Some ESTs were found only in CSCA and some only in CSBA. Finally, a comparative genomics research was performed with Fusarium graminearum, Aspergillus nidulans, Neurospora crassa and Saccharomyces bayanus. The results indicate that the fungus’ genes maybe have complicated variation at the nucleic acid level, but the proteins translated are still conservative. | ZHANG ShaoPeng FENG Hui LI XiaoYan JIN YongSan DONG Wei | 2010 | Chinese Science Bulletin2010，55，14： | 4 |
| 5 | Hydrolysis mechanism of carbendazim hydrolase from the strain Microbacterium sp.djl-6F显示文摘The carbendazim(MBC) hydrolyzing enzyme gene was cloned and heterologously expressed in Escherichia coli BL21(DE3) from a newly isolated MBC-degrading bacterium strain Microbacterium sp. strain djl-6F. High performance liquid chromatography-mass spectrometry(HPLC-MS)analysis revealed that purified MheI-6F protein catalyzes direct hydrolysis of MBC into2-aminobenzimidazole(2-AB) with a high turnover rate and moderate affinity(Kmof6.69 μmol/L and kcatof 160.88/min) without the need for any cofactors. The optimal catalytic condition of MheI-6F was identified as 45°C, pH 7.0. The enzymatic activity of MheI-6F was found to be diminished by metal ions, and strongly inhibited by sodium dodecyl sulfate(SDS).Through generating amino acid mutations in MheI-6F, Cys16 and Cys222 were identified as the catalytic groups that are essential for the hydrolysis of MBC. This is the first report on the biodegradation of MBC at the enzymatice level. | Ji Lei Shaopeng Wei Lijun Ren Shibin Hu Peng Chen | 2017 | Journal of Environmental Sciences2017，29，4： | 3 |
| 6 | Forest soil respiration and its heterotrophic and autotrophic components: Global patterns and responses to temperature and precipitation显示文摘 | Wang Wei Chen Weile Wang Shaopeng | 2010 | Soil Biology and Biochemistry2010，，8： | 2 |
| 7 | Broad negative thermal expansion operation-temperature window in antiperovskite manganese nitride with small crystallites显示文摘Using spark plasma sintering (SPS), Mn3Cu0.6Ge0.4N crystallites have been fabricated with different crystallite sizes, and their magnetic properties and thermal behaviors were systemically investigated. With decreasing crystallite size, the magnetic transition becomes increasingly slow, accompanied by broadening of the negative thermal expansion (NTE) operation-temperature window. The NTE operation-temperature window for the 12-nm crystallite sample reaches at 140 K, which is about 75% larger than that of the 74-nm crystallite sample. The magnetic properties and NTE operation-temperature window can be tuned by varying the crystallite size. This discovery will promote an even wider range of practical applications in precision devices. | Jie Tan Rongjin Huang Wei Wang Wen Li YuqiangZhao Shaopeng Li Yemao Han Chuanjun Huang Laifeng Li | 2015 | Nano Research2015，8，7： | 2 |
| 8 | Isolation and characterization of two novel antibacterial cyclic hexapeptides from Streptomyces alboflavus 313显示文摘 | JI Zhiqin QIAO Gang WEI Shaopeng | 2012 | Chemistry and Biodiversity2012，9，8： | 1 |
| 9 | Luminescence temperature and pressure studies of Zn 2 SiO 4 phosphors doped with Mn 2+ and Eu 3+ ions显示文摘 | Fuhai Su Baoshan Ma Kun Ding Guohua Li Shaopeng Wang Wei Chen Alan G. Joly David E. McCready | 2005 | Journal of Luminescence2005，，1： | 1 |
| 10 | Iso- lation, biological evaluation and 3D - QSAR studies of insecticidal/narcotic sesquiterpene polyol esters 显示文摘 | WEI Shaopeng JI Zhiqin ZHANG Huixiao | 2011 | J Mol Model2011，17，： | 1 |
| 11 | Synthesis andantibacterial activities of 1-alkyl-3-methacryloyl(acryloyl)ofbenzimidazolone(thione)derivatives显示文摘 | WEI Shaopeng WU Wenjun JI Zhiqin | 2012 | Int J Mol Sci2012，13，： | 1 |
| 12 | ASER:Animal Sex Reversal Database显示文摘Sex reversal,representing extraordinary sexual plasticity during the life cycle,not only triggers reproduction in animals but also affects reproductive and endocrine system-related diseases and cancers in humans.Sex reversal has been broadly reported in animals;however,an integrated resource hub of sex reversal information is still lacking.Here,we constructed a comprehensive database named ASER(Animal Sex Reversal)by integrating sex reversal-related data of 18 species from teleostei to mammalia.We systematically collected 40,018 published papers and mined the sex reversal-associated genes(SRGs),including their regulatory networks,from 1611 core papers.We annotated homologous genes and computed conservation scores for whole genomes across the 18 species.Furthermore,we collected available RNA-seq datasets and investigated the expression dynamics of SRGs during sex reversal or sex determination processes.In addition,we manually annotated 550 in situ hybridization(ISH),fluorescence in situ hybridization(FISH),and im-munohistochemistry(IHC)images of SRGs from the literature and described their spatial expression in the gonads.Collectively,ASER provides a unique and integrated resource for researchers to query and reuse organized data to explore the mechanisms and applications of SRGs in animal breeding and human health.The ASER database is publicly available at http://gffzz680c155e21ae46dbhw9ufbqn69x6n6fv0.ffgz.tsg.suse.edu.cn/. | Yangyang Li Zonggui Chen Hairong Liu Qiming Li Xing Lin Shuhui Ji Rui Li Shaopeng Li Weiliang Fan Haiping Zhao Zuoyan Zhu Wei Hu Yu Zhou Daji Luo | 2021 | Genomics, Proteomics & Bioinformatics2021，19，6： | 0 |
| 13 | Pluripotent stem cells secrete Activin A to improve their epiblast competency after injection into recipient embryos显示文摘 | Jinzhu Xiang Suying Cao Liang Zhong Hanning Wang Yangli Pei Qingqing Wei Bingqiang Wen Haiyuan Mu Shaopeng Zhang Liang Yue Genhua Yue Bing Lim Jianyong Han | 2018 | Protein & Cell2018，9，8： | 0 |
| 14 | Effects of Ulva prolifera dissipation on the offshore environment based on remote sensing images and field monitoring data显示文摘Outbreaks of Ulva prolifera have continued in the South Yellow Sea of China(SYS)since 2007,becoming a serious marine ecological disaster.Large amounts of U.prolifera drift to the coast of the Shandong Peninsula to dissipate under the action of southeast monsoons and ocean surface currents.This causes serious harm to the ecological environment and economic activities of coastal cities.To investigate the impact of U.prolifera dissipation,this study extracted the spatiotemporal distribution of U.prolifera in the SYS from 2012 to 2020 based on the Google Earth Engine.The outbreak cycle of U.prolifera was determined by fitting analysis of outbreak time and coverage area through MATLAB.This study also looked at the effect of U.prolifera dissipation on water quality through field monitoring data.The results showed that the growth curve of the U.prolifera has a significant Gaussian distribution.The U.prolifera dissipates in Haiyang,China,in July and August every year and affects the offshore environment.Water quality parameters of seawater at different depths had significant differences after the U.prolifera dissipation.Changes in pH,chemical oxygen demand,nitrite nitrogen,nitrate nitrogen,ammonia nitrogen,chlorophyll a,total phosphorus,and suspended solids were more significant in surface seawater than in deeper water.Changes in the concentrations of dissolved oxygen and total nitrogen were more significant in the deep seawater(1.63 and 1.1 times higher than those in the surface seawater,respectively).The dissipation of U.prolifera releases a large amount of carbon and nitrogen into the seawater,which provides rich nutrients for phytoplankton and may cause secondary disasters such as red tide.These findings are useful for further understanding the rules of U.prolifera dissipation,as well as preventing and controlling green tide disasters. | Longxiao Zheng Mengquan Wu Jie Zhao Shaopeng Luan Dongliang Wang Wei Jiang Mingyue Xue Jiayan Liu Yating Cui | 2023 | Acta Oceanologica Sinica2023，42，6： | 0 |
| 15 | Modulated-ISRJ rejection using online dictionary learning for synthetic aperture radar imagery显示文摘In electromagnetic countermeasures circumstances,synthetic aperture radar(SAR)imagery usually suffers from severe quality degradation from modulated interrupt sampling repeater jamming(MISRJ),which usually owes considerable coherence with the SAR transmission waveform together with periodical modulation patterns.This paper develops an MISRJ suppression algorithm for SAR imagery with online dictionary learning.In the algorithm,the jamming modulation temporal properties are exploited with extracting and sorting MISRJ slices using fast-time autocorrelation.Online dictionary learning is followed to separate real signals from jamming slices.Under the learned representation,time-varying MISRJs are suppressed effectively.Both simulated and real-measured SAR data are also used to confirm advantages in suppressing time-varying MISRJs over traditional methods. | WEI Shaopeng ZHANG Lei LU Jingyue LIU Hongwei | 2024 | Journal of Systems Engineering and Electronics2024，35，2： | 0 |
| 16 | Effect on surgery outcomes owing to the interval between onset of symptoms and surgery of patients with acute type A aortic dissection显示文摘Background:This study aimed to identify whether the interval from onset of symptoms to surgery affects the outcomes of surgery in patients with acute type A aortic dissection(AAAD).Methods:This study retrospectively examined 249 patients with AAAD who underwent Sun’s procedure.All patients were divided into 2 groups,hyperacute and acute,according to the interval from onset of symptoms to surgery.The primary endpoint was all-cause early mortality,and the secondary endpoint was early reoperation.Results:The surgery time,cardiopulmonary bypass time,clamp time,and selective cerebral perfusion time were not significantly different between the 2 groups.The intensive care unit length of stay and duration of mechanical ventilation of the 2 groups were 185.50hours versus 185.00hours(P=0.970)and 41.50hours versus 44.00hours(P=0.678),respectively.There were 52 early deaths:29 in the hyperacute group and 23 in the acute group(21.6%vs.20.0%,P=0.751).The incidence of reoperation was 0.7% and 0.9%(P>0.999),respectively.The incidence rates of postoperative acute heart failure(AHF),acute respiratory failure(ARF),nervous dysfunction,and acutekidney injury were37.3% versus 25.2%(P=0.041),51.5%versus51.3%(P=0.976),13.4% versus 7.0%(P=0.096),and 37.3% versus 37.4%(P=0.990),respectively.Multivariable analysis indicated that surgery in the hyperacute phase might be an independent risk factor for AHF(OR:1.765;95%CI:1.021–3.052;P=0.042).Conclusion:Surgery in the hyperacute phase of AAAD was associated with postoperative AHF.Therefore,early medical management or interventional therapy for complications before surgery performed by experienced surgeons is recommended,especially in the hyperacute phase. | Shouming Li Duoliang Wei Zhenhua Wang Han Song Shaopeng Cheng Xin Zhao | 2022 | Emergency and Critical Care Medicine2022，2，2： | 0 |
| 17 | Perlecan/Hspg2 Deficiency Impairs Bone’s Calcium Signaling and Associated Transcriptome in Response to Mechanical Loading显示文摘Perlecan,a heparan sulfate proteoglycan,acts as a mechanical sensor for bone to detect external loading.Deficiency of perlecan increases the risk of osteoporosis in patients with Schwartz-Jampel Syndrome(SJS)and attenuates loading4nduced bone formation in perlecan deficient mice(Hypo).Considering that intracellular calcium[Ca2+]i is an ubiquitous messenger controlling numerous cellular processes including mechanotransduction,we hypothesized that perlecan deficiency impairs bone’s calcium signaling in response to loading.To test this,we performed real-time[Ca2+]i imaging on in situ osteocytes of adult murine tibiae under cyclic loading(8 N,Figure 1).Relative to wild type(WT),Hypo osteocytes showed decreases in the overall[Ca2+]i response rate(-58%),calcium peaks(-33%),cells with multiple peaks(-53%),peak magnitude(-6.8%),and recovery speed to baseline(-23%).RNA sequencing and pathway analysis of tibiae from mice subjected to one or seven days of unilateral loading demonstrated that perlecan deficiency significantly suppressed the calcium signaling,ECM-receptor interaction,and focal adhesion pathways following repetitive loading.Defects in the endoplasmic reticulum(ER)calcium cycling regulators such as Ryr1/ryanodine receptors and Atp2a1/Sercal calcium pumps were identified in Hypo bones.Taken together,impaired calcium signaling may contribute to bone’s reduced anabolic response to loading,underlying the osteoporosis risk for the SJS patients. | Shaopeng Pei Sucharitha Parthasarathy Ashutosh Parajuli Jerahme Martinez Mengxi Lv Sida Jiang Danielle Wu Shuo Wei XLucas Lu Mary CFarach-Carson Catherine BKirn-Safran Liyun Wang | 2019 | 医用生物力学2019，34，A01： | 0 |
| 18 | Single-atom catalysts modified by molecular groups for electrochemical nitrogen reduction显示文摘Electrochemical nitrogen reduction reaction(eNRR)is one of the most important chemical reactions for the production of ammonia under ambient environment.However,the lack of in-depth understanding of the structure-activity relationship impedes the development of high-performance catalysts for ammonia production.Herein,the density functional theory(DFT)calculations are performed to reveal the structure–activity relationship for the single-atom catalysts(SACs)supported on g-C_(3)N_(4),which is modified by molecular groups(i.e.,H,O,and OH).The computational results demonstrate that the W-based SACs are beneficial to produce ammonia with a low limiting potential(UL).Particularly,the W-OH@g-C_(3)N_(4) catalyst exhibits an ultralow UL of−0.22 V for eNRR.And the competitive eNRR selectivity can be identified by the dominant*N2 adsorption free energy than that of*H.Our findings provide a theoretical basis for the synthesis of efficient catalysts to produce ammonia. | Zengxi Wei Yuchang Liu Hongjie Liu Shaopeng Wang Minchen Hou Liwei Wang Dong Zhai Shuangliang Zhao Kefu Yu Shaolong Zhang | 2022 | Nano Research2022，15，10： | 0 |
| 19 | Bending and wave propagation analysis of axially functionally graded beams based on a reformulated strain gradient elasticity theory显示文摘A new size-dependent axially functionally graded(AFG) micro-beam model is established with the application of a reformulated strain gradient elasticity theory(RSGET). The new micro-beam model incorporates the strain gradient, velocity gradient,and couple stress effects, and accounts for the material variation along the axial direction of the two-component functionally graded beam. The governing equations and complete boundary conditions of the AFG beam are derived based on Hamilton's principle. The correctness of the current model is verified by comparing the static behavior results of the current model and the finite element model(FEM) at the micro-scale. The influence of material inhomogeneity and size effect on the static and dynamic responses of the AFG beam is studied. The numerical results show that the static and vibration responses predicted by the newly developed model are different from those based on the classical model at the micro-scale. The new model can be applied not only in the optimization of micro acoustic wave devices but also in the design of AFG micro-sensors and micro-actuators. | Shaopeng WANG Jun HONG Dao WEI Gongye ZHANG | 2023 | Applied Mathematics and Mechanics(English Edition)2023，44，10： | 0 |
| 20 | Ball-milling Synthesis of Single-atom Cu Anchored on N-Doped Carbon for Mimicking Peroxidase显示文摘Nanozymes have shown great potential for life sciences owing to their distinct advantages,such as low cost and high stability,compared with natural enzymes.Despite significant progress,state-of-art nanozymes commonly suffer from relatively low specific activities.Herein,we propose a promising to address this issue by creating single-atom nanozymes.A ball-milling-assisted strategy has been developed to induce the transformation of Cu species from bulk to single atoms.The highly-simplified steps allow a large-scale synthesis,that over 4.2 g of single-atom Cu-N doped carbon nanozymes can be achieved in one pot.It exhibits a remarkably improved peroxidase-like activity and stability compared with N doped C anchored Cu nanoparticles.Further experimental firmly reveals the crucial role of the single-atom Cu site that can generate more active·OH species for boosting the catalytic process. | XU Henan ZHANG Lingling WANG Huilin ZHANG Shaopeng LI Wei WANG Xiao SONG Shuyan WANG Daguang SHI Zhan | 2023 | Chemical Research in Chinese Universities2023，39，6： | 0 |
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