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| 1 | Investigation on gas generation of Li 4 Ti 5 O 12 /LiNi 1/3 Co 1/3 Mn 1/3 O 2 cells at elevated temperature显示文摘 | Kai Wu Jun Yang Yang Liu Yao Zhang Chenyun Wang Jinmei Xu Feng Ning Deyu Wang | 2013 | Journal of Power Sources2013，，： | 2 |
| 2 | Preperation of Er2O3 thin films by dipping process显示文摘 | Wang Chenyun | 2002 | Journal of Materials Science:Materials in electronics2002，13，： | 1 |
| 3 | Distinct effects of mutations on biophysical properties of human prion protein monomers and oligomers显示文摘 | Yuanhui Yu Ziyao Yu Zhen Zheng Huilin Wang Xueji Wu Chenyun Guo Donghai Lin | 2016 | Acta Biochimica et Biophysica Sinica2016，48，11： | 1 |
| 4 | Studies of V doping for the LiFePO4-based Li Ion batteries显示文摘 | Hua N Wang Chenyun Kang Xneya | | Journal of Alloys and Compounds0，503，1： | 1 |
| 5 | Biophysical and functional characterizations of recombinant RimI acetyltransferase from Mycobacterium tuberculosis显示文摘Nα-acetylation is a universal protein modification related to a wide range of physiological processes in eukaryotes and prokaryotes. RimI, an Nα-acetyltransferase in Mycobacterium tuberculosis, is responsible for the acetylation of the α-amino group of the N-terminal residue in the ribosomal protein S18. Despite growing evidence that protein acetylation may be correlated with the pathogenesis of tuberculosis, no structural information is yet available for mechanistically understanding the MtRimI acetylation. To enable structural studies for MtRimI, we constructed a serial of recombinant MtRimI proteins and assessed their biochemical properties. We then chose an optimal construct MtRimIC21A4-153 and expressed and purified the truncated high-quality protein for further biophysical and functional characterizations. The 2D 1H-15N heteronuclear single quantum coherence spectrum of MtRimIC21A4-153 exhibits wider chemical shift dispersion and favorable peak isolation, indicating that MtRimIC21A4-153 is amendable for further structural determination. Moreover, bio-layer interferometry experiments showed that MtRimIC21A4-153 possessed similar micromolar affinity to full-length MtRimI for binding the hexapeptide substrate Ala-Arg-Tyr-Phe-Arg-Arg. Enzyme kinetic assays also exhibited that MtRimIC21A4-153 had almost identical enzymatic activity to MtRimI, indicating insignificant influence of the recombinant variations on enzymatic functions. Furthermore, binding sites of the peptide were predicted by molecular docking approach, suggesting that this substrate binds to MtRimI primarily through electrostatic and hydrogen bonding interactions. Our results lay a foundation for the further structural determination and dynamics detection of MtRimI. | Meijing Hou Jie Zhuang Shihui Fan Huilin Wang Chenyun Guo Hongwei Yao Donghai Lin Xinli Liao | 2019 | Acta Biochimica et Biophysica Sinica2019，51，9： | 0 |
| 6 | Study on the correlation between bandage bandage and pressure therapy of lymphedema显示文摘To explore the effects of bandage technology and pressure therapy in breast cancer with lymphedema.Methods A total of 91 patients,with breast cancer-related lymphedema and hospitalized in a third-class hospital in Jiangsu Province from May to December,2018,were selected and sequenced in class“ABC”in the order of entry.The patients in class“A”were enrolled in“8”shaped bandaging group.The patients in class“B”were enrolled in“5”spiral bandaging group.The patients in class“C”were enrolled in combined bandage group.The primary outcome was the degree of limb swelling used by multiple-frequency bioelectrical impedance analysis.Secondary outcomes were the amount of bandage losses,counting 24-hour bandage loose occurrences and patients satisfaction.All outcomes were evaluated both before and after treatment.Results After treatment,there was a significant difference in the regression of limb swelling in all three groups(P<0.05).However,the largest amplitude of regression was observed in combined bandage group(limb circumference ratio:53%and tissue water ratio:68%).Although“8”bandaging group had large material,bandage cost and minimum comfort,the bandage has good stability and no case of 24-hour bandage loose occurrences was found in this group.The“5”partial binding group had an advantage in the amount of bandage losses and comfort level,but among which 3 patients had the phenomenon of loose bandage at the joint.The combined bandage group had the best therapeutic effect in limb swelling and patients satisfaction(P<0.05).Conclusion The combined bandage group significantly decreased the degree of limb swelling and 24-hour bandage loose occurrences.Importantly,this treatment improved the degree of patients’satisfaction.The treatment of“8”shaped bandaging combined with spiral bandaging is worthy of clinical promotion. | WANG Bei Li Haihong LOU Qingqing LI Jianing WANG Xiaoyuan XU Chenyun | 2020 | 中西医结合护理(中英文)2020，6，10： | 0 |
| 7 | Recombinant expression, biophysical and functional characterization of ClpS from Mycobacterium tuberculosis显示文摘Intracellular proteolysis is attracting more and more attention for its unique and important character in Mycobacterium tuberculosis(Mt).The ClpS protein from Mt(MtClpS)plays a critical role in intracellular proteolysis by recognizing N-end rule substrates,which makes it become a potential target for antibacterial drugs.However,the molecular mechanism of MtClpS recognizing N-end rule substrates remains unclear.Preparation of highly concentrated and pure MtClpS protein is a prerequisite for further structural and functional studies.In the present work,we tried several fusion tags and various expression conditions to maximize the production of MtClpS in Escherichia coli.We established an efficient approach for preparing the MtClpS protein with a high yield of 24.7 mg/l and a high purity of 98%.After buffer screening,we obtained a stable MtClpS protein sample concentrated at 0.63 mM in the presence of glycerol,l-Arginine,and l-Glutamate.Moreover,circular dichroism characterization indicated that the secondary structure of MtClpS consists of 38%α-helix and 24%β-sheet.The 2D 1H-15N HSQC nuclear magnetic resonance spectrum showed a good dispersion of resonance peaks with uniform intensity,indicating that the purified MtClpS protein was well folded and conformationally homogeneous.Isothermal titration calorimetry experiments revealed significant interactions of MtClpS with N-end rule peptides beginning with Leu,Tyr,Trp,or Phe.Furthermore,residues D34,D35,and H66 were confirmed as key residues for MtClpS recognizing the N-end rule peptide.The successful expression and biophysical characterization of MtClpS enabled us to gain insight into the molecular mechanism of MtClpS recognizing N-end rule substrates.The obtained stable and pure recombinant MtClpS will enable future inhibitor screening experiments. | Chenyun Guo Yihang Xiao Fangkai Bs Weiliang Lin Huilin Wang Hongwei Yao Donghai Lin | 2019 | Acta Biochimica et Biophysica Sinica2019，51，11： | 0 |
| 8 | MAGNETIC PHASE TRANSITIONS AND MAGNETIC PROPERTIES OF Nd_3(Fe,Mo)_(29) COMPOUND AND ITS NITRIDE显示文摘MAGNETICPHASETRANSITIONSANDMAGNETICPROPERTIESOFNd3(Fe,Mo)29COMPOUNDANDITSNITRIDE①PanHongge,WangXinhua,ChenYunandChenChangpinD... | Pan Hongge, Wang Xinhua, ChenYun and Chen ChangpinDepartment of Materials Science and Engineering,Zhejiang University, Hangzhou 310027, P. R. ChinaYang FumingState Key Laboratory for Magnetism,Institute of Physics, Chinese Academy of Sciences, | 1998 | 中国有色金属学会会刊:英文版1998，8，2： | 0 |
| 9 | Role of melatonin receptor 1B gene polymorphism and its effect on the regulation of glucose transport in gestational diabetes mellitus显示文摘Melatonin receptor 1B(MT2,encoded by the MTNR1B gene),a high-affinity receptor for melatonin,is associated with glucose homeostasis including glucose uptake and transport.The rs10830963 variant in the MTNR1B gene is linked to glucose metabolism disorders including gestational diabetes mellitus(GDM);however,the relationship between MT2-mediated melatonin signaling and a high birth weight of GDM infants from maternal glucose abnormality remains poorly understood.This article aims to investigate the relationship between rs10830963 variants and GDM development,as well as the effects of MT2 receptor on glucose uptake and transport in trophoblasts.TaqMan-MGB(minor groove binder)probe quantitative realtime polymerase chain reaction(qPCR)assays were used for rs10930963 genotyping.MT2 expression in the placenta of GDM and normal pregnant women was detected by immunofluorescence,western blot,and qPCR.The relationship between MT2 and glucose transporters(GLUTs)or peroxisome proliferator-activated receptorγ(PPARγ)was established by western blot,and glucose consumption of trophoblasts was measured by a glucose assay kit.The results showed that the genotype and allele frequencies of rs10830963 were significantly different between GDM and normal pregnant women(P<0.05).The fasting,1-h and 2-h plasma glucose levels of G-allele carriers were significantly higher than those of C-allele carriers(P<0.05).Besides,the protein and messenger RNA(mRNA)expression of MT2 in the placenta of GDM was significantly higher than that of normal pregnant women(P<0.05).Melatonin could stimulate glucose uptake and GLUT4 and PPARγprotein expression in trophoblasts,which could be attenuated by MT2 receptor knockdown.In conclusion,the rs10830963 variant was associated with an increased risk of GDM.The MT2 receptor is essential for melatonin to raise glucose uptake and transport,which may be mediated by PPARγ. | Lijie WEI Yi JIANG Peng GAO Jingyi ZHANG Xuan ZHOU Shenglan ZHU Yuting CHEN Huiting ZHANG Yuanyuan DU Chenyun FANG Jiaqi LI Xuan GAO Mengzhou HE Shaoshuai WANG Ling FENG Jun YU | 2023 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2023，24，1： | 0 |
| 10 | Crystal structure of the polyketide cyclase from Mycobacterium tuberculosis显示文摘About 40%of proteins are classified as conserved hypothetical proteins in Mycobacterium tuberculosis(TB).Identification and characterization of these proteins are beneficial to understand the pathogenesis of TB and exploiting novel drugs for TB treatments.The polyketide cyclase,a protein from M.tuberculosis(MtPC)has been annotated as a hypothetical protein in Uniprot database.Sequence analysis shows that the MtPC belongs to the NTF2-like superfamily proteins with diverse functions.Here,we determined the crystal structure of MtPC at a resolution of 2.4Åand measured backbone relaxation parameters for the MtPC protein.MtPC exists as a dimer in solution,and each subunit contains a six-stranded mixedβ-sheet and threeαhelixes which are arranged in the orderα1-α2-β1-β2-α3-β3-β4-β5-β6.The NMR dynamics analysis showed that the overall structure of MtPC is highly rigid on ps-ns time scales.Furthermore,we predicted the potential function of MtPC based on the crystal structure.Our results lay the basis for further exploiting and mechanistically understanding the biological functions of MtPC. | Jie Zhuang Shihui Fan Chenyun Guo Liubin Feng Huilin Wang Donghai Lin Xinli Liao | 2022 | Acta Biochimica et Biophysica Sinica2022，54，4： | 0 |
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