|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Homogeneous PLGA-lipid nanoparticle as a promising oral vaccine delivery system for ovalbumin显示文摘In this study,a polymeric lipid nanoparticle(NP)(simplified as Lipid NP)was reported as a promising oral vaccine delivery system.The Lipid NPs composed of a hydrophobic polymeric poly(D,L-lactide-co-glycolide)(PLGA)core and a surface coating of lipid monolayer.Membrane emulsification technique was used to obtain uniform-sized Lipid NPs.Ovalbumin(OVA)was used as a model vaccine.Compared with the pure PLGA NPs,the Lipid NPs achieved higher loading capacity(LC)and entrapment efficiency(EE)for the encapsulated OVA.An in vitro oral release profile showed that the OVA-Lipid NPs were with lower initial burst and could protect the loaded OVA from the harsh gastrointestinal(GI)environment for a long time.In addition,a human microfold cell(M-cell)transcytotic assay demonstrated that due to a lipid layer structure on the particle surface,the Lipid NPs showed higher affinity to the M-cells.Since the M-cell in the intestinal epithelium played an important role in particle transportation as well as intimately associated with the underlying immune cells,the OVA-Lipid NPs effectively induced mucosal and humoral immune responses. | Tongtong Ma Lianyan Wang Tingyuan Yang Guanghui Ma Siling Wang | 2014 | Asian Journal of Pharmaceutical Sciences2014，9，3： | 4 |
| 2 | A thermosensitive hydrogel based on quaternized chitosan and poly(ethylene glycol) for nasal drug delivery system显示文摘 | Wu Jie Wei Wei Wang Lianyan | 2007 | Biomaterials2007，28，13： | 1 |
| 3 | Surface charge of PLA microparticles in regulation of antigen loading,macrophage phagocytosis and activation,and immune effects in vitro显示文摘The use of microparticles(MPs) as adjuvants has attracted increasing interest in vaccine delivery systems.Many physiochemical characteristics of MPs including hydrodynamic size,surface properties,and morphology can regulate the immune response.Surface charge is an important characteristic of MPs,but how it affects their adjuvanticity remains unknown.In this study,we prepared uniform-sized polylactide MPs coated with various polymers of different positive charge,and investigated how the surface charge affected antigen loading and macrophage phagocytosis and activation in vitro.A higher surface charge greatly enhanced antigen loading and antigen internalization into macrophages,promoted the expression of MHC 11 and CD80,and increased the secretion level of TNF-a.Taken together,these results indicated that surface charge was an important parameter for improving the adjuvanticity of MPs. | Yuying Liu Xiaoming Chen Lianyan Wang Tingyuan Yang Qipeng Yuan Guanghui Ma | 2014 | Particuology2014，12，6： | 1 |
| 4 | In situ magneti zation technique for synthesis of magnetic polymer microspheres 显示文摘 | Lianyan Wang Tingyuau Yang Qiang Li | 2013 | Powder Technology2013，235，： | 1 |
| 5 | Preparation and Characterization of Uniform-sized Chitosan Micro- spheres Containing Insulin by Membrane Emulsification and a Two-step Solidification Process显示文摘 | Wang Lianyan Gu Yonghong Zhou Qingzhu | 2006 | Colloids and Surfaces B: Biointerfaces2006，50，2： | 1 |
| 6 | Preparation and characterization of uniform-sized chitosan microspheres containing insulin by membrane emulsification and a two-step solidification process 显示文摘 | Wang Lianyan Gu Yonghong Zhou Qingzhu | 2006 | Colloids and Surfaces B : Biointerfaces2006，50，： | 1 |
| 7 | Preparation of uniform-sized pH-sensitive quatemized chitosan microsphere by combining membrane emulsification technique and thermal-gelation method显示文摘 | Wu Jie Wei Wei Wang Lianyan | 2008 | Colloids and Surfaces B: Biointerfaces2008，63，： | 1 |
| 8 | Preparation of uniform sized chitosan microspheres by membrane emulsification technique and application as a carrier of protein drug显示文摘 | WANG LIANYAN MA GUANGHUI SU ZHIGUO | 2005 | Journal of Controlled Release2005，106，： | 1 |
| 9 | A thermosensitive hydrogel based on quaternized chitosan and poly(ethylene glycol) for nasal drug delivery system 显示文摘 | Wu Jie Wei Wei Wang Lianyan | 2007 | Biomaterials2007，28，13： | 1 |
| 10 | A molecularly imprinted polymer-coated nanocomposite of magnetic nanoparticles for estrone recognition显示文摘 | Xin Wang Lianyan Wang Xiwen He Yukui Zhang Langxing Chen | 2008 | Talanta2008，，2： | 1 |
| 11 | A molecularly imprinted polymer-coated nanocomposite of magnetic nanopartieles for estrone recognition显示文摘 | WANG Xin WANG Lianyan HE Xiwen | 2009 | Talanta2009，78，2： | 1 |
| 12 | Biomass increment and mortality losses in tropical secondary forests of Hainan, China显示文摘Secondary forests, created after heavy logging,are an important part of China's forests. We investigated forest biomass and its accumulation rate in 38 plots in a tropical secondary forest on Hainan Island. These secondary forests are moderate carbon sinks, averaging1.96–2.17 t C ha-1 a-1. Biomass increment is largely by medium-sized(10–35 m) trees. Tree mortality accounts for almost 30% of the biomass and plays a negligible role in biomass accumulation estimates. Mortality rate is highly dependent on tree size. For small trees and seedlings, it is related to competition due to elevated irradiance after logging. Regarding prospective biomass and rates of accumulation, recovery is not as rapid as in secondary forests of cleared land. Therefore, tropical forests are susceptible to logging operations and need careful forest management. | Junfu Zhao Chunsheng He Chunlin Qi Xu Wang Haiyan Deng Chunxin Wang Haiwei Liu Lianyan Yang Zhenghong Tan | 2019 | Journal of Forestry Research2019，30，2： | 1 |
| 13 | Uniform-sized silicone oil microemulsions : Preparation, investigation of stabilityand deposition on hair surface显示文摘 | Habiba Nazir Piping Lv Lianyan Wang | 2011 | Journal of Colloid and Interface Science2011，364，1： | 1 |
| 14 | Dual-function baicalin and baicalin-loaded poly(lactic-co-glycolic acid) nanoparticles: Immune activation of dendritic cells and arrest of the melanoma cell cycle at the G2/M phase显示文摘 | Huimei Wang Shulan Han Lianyan Wang Tingyuan Yang Guifeng Zhang Lian Yu Yue Zhao | 2018 | Particuology2018，16，2： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员