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您的检索式：作者名="Wanpeng Cheng"
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| 1 | SlMYB75,an MYB-type transcription factor,promotes anthocyanin accumulation and enhances volatile aroma production in tomato fruits显示文摘Genetic manipulation of genes to upregulate specific branches of metabolic pathways is a method that is commonly used to improve fruit quality.However,the use of a single gene to impact several metabolic pathways is difficult.Here,we show that overexpression of the single gene SlMYB75(SlMYB75-OE)is effective at improving multiple fruit quality traits.In these engineered fruits,the anthocyanin content reached 1.86mg g−1 fresh weight at the red-ripe stage,and these SlMYB75-OE tomatoes displayed a series of physiological changes,including delayed ripening and increased ethylene production.In addition to anthocyanin,the total contents of phenolics,flavonoids and soluble solids in SlMYB75-OE fruits were enhanced by 2.6,4,and 1.2 times,respectively,compared to those of wild-type(WT)fruits.Interestingly,a number of aroma volatiles,such as aldehyde,phenylpropanoid-derived and terpene volatiles,were significantly increased in SlMYB75-OE fruits,with some terpene volatiles showing more than 10 times higher levels than those in WT fruits.Consistent with the metabolic assessment,transcriptomic profiling indicated that the genes involved in the ethylene signaling,phenylpropanoid and isoprenoid pathways were greatly upregulated in SlMYB75-OE fruits.Yeast one-hybrid and transactivation assays revealed that SlMYB75 is able to directly bind to the MYBPLANT and MYBPZM cis-regulatory elements and to activate the promoters of the LOXC,AADC2 and TPS genes.The identification of SlMYB75 as a key regulator of fruit quality attributes through the transcriptional regulation of downstream genes involved in several metabolic pathways opens new avenues towards engineering fruits with a higher sensory and nutritional quality. | Wei Jian Haohao Cao Shu Yuan Yudong Liu Juanfang Lu Wang Lu Ning Li Jianhui Wang Jian Zou Ning Tang Chan Xu Yulin Cheng Yanqiang Gao Wanpeng Xi Mondher Bouzayen Zhengguo Li | 2019 | Horticulture Research2019，6，1： | 39 |
| 2 | Seismic Behaviour of Beam-Column Joints of Precast and Partial Steel Reinforced Concrete显示文摘A beam-column joint of precast and partial steel reinforced concrete( PPSRC) is proposed for precast reinforced concrete frames. The PPSRC consists of partial steel and reinforced concrete. The partial steel is located in the core joint region and the connections between concrete members. This paper presents an experimental study of a series of PPSRC specimens. These specimens are tested under low cyclic loading.Experimental results demonstrate that the bearing capacity of the PPSRC specimens is 3 times that of the ordinary reinforced concrete( RC) beam-column joints. The strength and stiffness degradation rates are slower compared with that of the RC beam-column joints. In addition,the strength of the core joint region and the connections is higher than other parts of the PPSRC specimens. Beam failure occurs firstly for the PPSRC specimens,followed by column failure and connections failure. The failure of the core joint region occurs finally.Test results show that the seismic performance of the PPSRC is better than that of the ordinary RC beam-column joints. | Wanpeng Cheng Licheng Wang Yupu Song Jun Wang | 2015 | Journal of Harbin Institute of Technology(New Series)2015，22，2： | 1 |
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