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| 1 | Role and effect of vein-transplanted human umbilical cord mesenchymal stem cells in the repair of diabetic foot ulcers in rats显示文摘Diabetic foot ulcer(DFU)is one of diabetic complications,which is frequently present and tormented in diabetes mellitus.Most multipotent mesenchymal stromal cells(MSCs)are capable of immune evasion,providing an allogeneic,ready-to-use,cell product option for therapeutic applications.The beneficial effect of MSCs for the treatment of a variety of traumatic injuries,such as open wounds,has been extensively explored.In this study,a rat DFU model was used to simulate the pathophysiology of clinical patients and to investigate the localization of human umbilical cord mesenchymal stem cells(hUC-MSCs)after intravenous transplantation and its role in DFU healing,so as to evaluate the potential of hUC-MSCs in the treatment of DFU.The diabetic rat model was established by streptozotocin injection,which was used to create full-thickness foot dorsal skin wounds to mimic DFU by a 6-mm skin biopsy punch and a Westcott scissor.The hUC-MSCs were transplanted through femoral vein,and the ulcer cicatrization situation and the fate of hUC-MSCs were evaluated.Our data suggest that intravenously transplantated hUC-MSCs have the ability to migrate and locate to the wound tissue and are helpful to wound healing in DFU rats,partly by regulating inflammation,trans-differentiation and providing growth factors that promote angiogenesis,cell proliferation and collagen deposition.Herein,we demonstrate that hUC-MSC transplantation is able to accelerate DFU healing in rats and transplantation of exogenous stem cells may be a potential strategy for clinical application in DFUs. | Rongfeng Shi Weishuai Lian Yinpeng Jin Chuanwu Cao Shilong Han Xiaohu Yang Suming Zhao Maoquan Li Hui Zhao | 2020 | Acta Biochimica et Biophysica Sinica2020，52，6： | 13 |
| 2 | MiR-31 regulates the function of diabetic endothelial progenitor cells by targeting Satb2显示文摘Endothelial 与糖尿病在病人失灵被知道导致脉管的疾病,并且 endothelial 祖先房间(EPC ) 为脉管的内皮细胞层的功能的保藏是不可缺少的。MicroRNA-31 (miR-31 ) 被发现了能调制干细胞的区别。然而, miR-31 怎么在糖尿病的 EPC 工作,仍然是不清楚的。这研究的目的是调查 miR-31 怎么调整糖尿病的 EPC 功能。在当前的学习, miR-31 表示在正常、糖尿病的 EPC 之间被比较。Satb2 根据计算预言在 EPC 作为 miR-31 的一个机能上地相关的目标被认出。我们也以它的 anti-apoptotic 效果探索了 miR-31 的角色。在 miR-31 表示的显著举起在糖尿病的 EPC 被发现,并且这提高的表情在高葡萄糖下面导致了压制的房间增长。miR-31 指向 Satb2,这也被发现,导致 anti-apoptotic 效果和 EPC 的功能的维护。而且,展出的 Satb2 击倒在健康、糖尿病的题目的 EPC 的增长和移植上的禁止的效果,它显示出象 miR-31 overexpression 的一样的趋势。相反地, Satb2 的 overexpression 显示出相反的效果。而且, Satb2 的 overexpression 在健康、糖尿病的题目稀释了 miR-31-induced 迁居和形成殖民地的能力减小和 EPC 的 apoptosis 正式就职。在糖尿病的 EPC,提高了葡萄糖水平被发现到起来调整 miR-31 表示,它接着提高了 EPC 的 malfunction 和死亡。在结论,我们的结果显示 miR-31 的起来规定可以由指向 Satb2 在糖尿病位于 endothelial 机能障碍下面。 | Weishuai Lian Xiaoxiao Hu Rongfeng Shi Shilong Han Chuanwu Cao Kun Wang Maoquan Li | 2018 | Acta Biochimica et Biophysica Sinica2018，50，4： | 7 |
| 3 | MicroRNA-182 promotes pancreatic cancer cell proliferation and migration by targeting β-TrCP2显示文摘胰腺的癌症是好攻击的恶意。中部的幸存率仍然保持低,显示新奇 biomarkers 和治疗学的目标的鉴定是批评的。这里,我们在胰腺的癌症开发检验了 microRNA-182 (miR-182 ) 的角色。人的胰腺的癌症标本和房间线的分析证明 miR-182 是在胰腺的癌症的 overexpressed 并且支持肿瘤增长和侵略。-TrCP2 作为 miR-182 的一个直接目标被证实。-TrCP2 的 Silencing 增加了类似于 miR-182 overexpression 的 -catenin, 的层次。-TrCP2 的宫外的表示禁止了 -catenin 发信号的 miR-182-induced 激活。由 -TrCP2 的 miR-182 和它的颠倒的 oncogenic 效果在 vivo 被证实。这研究建议 -TrCP 和 miR-182 可以为胰腺的癌症的早察觉和治疗是可能的 biomarkers 和目标。 | Shi Wang Jiansong Ji Jingjing Song Xue Li Shilong Han Weishuai Lian Chuanwu Cao Xiaoping Zhang Maoquan Li | 2016 | Acta Biochimica et Biophysica Sinica2016，48，12： | 4 |
| 4 | Stretchable and self-healable hydrogel artificial skin显示文摘Hydrogels have emerged as promising materials for the construction of skin-like mechanical sensors.The common design of hydrogel-based artificial skin requires a dielectric sandwiched between two hydrogel layers for capacitive sensing.However,such a planar configuration limits the sensitivity,stretchability and self-healing properties.Here,we report the design of single-layer composite hydrogels with bulk capacitive junctions as mechanical sensors.We engineer dielectric peptide-coated graphene(PCG)to serve as homogenously dispersed electric double layers in hydrogels.Any mechanical motions that alter the microscopic distributions of PCG in the hydrogels can significantly change the overall capacitance.We use peptide self-assembly to render strong yet dynamic interfacial interactions between the hydrogel network and graphene.The resulting hydrogels can be stretched up to 77 times their original length and self-heal in a few minutes.The devices can effectively sense strain and pressure in both air and aqueous environments,providing tremendous opportunities for next-generation iontronics. | Bin Xue Hui Sheng Yongqiang Li Lan Li Weishuai Di Zhengyu Xu Linjie Ma Xin Wang Haoting Jiang Meng Qin Zhibo Yan Qing Jiang Jun-Ming Liu Wei Wang Yi Cao | 2022 | National Science Review2022，9，7： | 2 |
| 5 | Machine learning enabled prediction and process optimization of VFA production from riboflavin-mediated sludge fermentation显示文摘Riboflavin is a redox mediator that promotes volatile fatty acids(VFAs)production from waste activated sludge(WAS)and is a promising method for WAS reuse.However,time-and labor-consuming experiments challenge obtaining optimal operating conditions for maximal VFA production.In this study,three machine learning(ML)models were developed to predict the VFAs production from riboflavin-mediated WAS fermentation systems.Among the three tested ML algorithms,eXtreme Gradient Boosting(XGBoost)presented the best prediction performance and excellent generalization ability,with the highest testing coefficient of determination(R^(2)of 0.93)and lowest root mean square error(RMSE of 0.070).Feature importance analysis and their interactions using the Shepley Additive Explanations(SHAP)method indicated that pH and soluble protein were the top two input features for the modeling.The intrinsic correlations between input features and microbial communities corroborated this deduction.On the optimized ML model,genetic algorithm(GA)and particle swarm optimization(PSO)solved the optimal solution of VFA output,predicting the maximum VFA output as 650 mg COD/g VSS.This study provided a data-driven approach to predict and optimize VFA production from riboflavin-mediated WAS fermentation. | Weishuai Li Jingang Huang Zhuoer Shi Wei Han Ting Lü Yuanyuan Lin Jianfang Meng Xiaobing Xu Pingzhi Hou | 2023 | Frontiers of Environmental Science & Engineering2023，17，11： | 0 |
| 6 | Exogenous FGF-2 improves biological activity of endothelial progenitor cells exposed to high glucose conditions显示文摘Purpose: To investigate the effects of exogenous basic fibroblast growth factor-2(FGF-2) on the biological activity of endothelial progenitor cells(EPCs) exposed to high glucose conditions. Materials and Methods: 1) Bone marrow EPCs from C57BL/6 mice were isolated and cultured in vitro. EPC purity was identified by flow cytometry and immunofluorescence staining. 2) Apoptosis was detected by TUNEL assay. Migration and tube formation ability was detected by Transwell chamber and Matrigel assays, respectively. The expression and activation of β-catenin was detected by Western blot. 3) Doppler flowmetry was used to detect the effect of FGF2 on blood flow recovery in ischemic hind limbs of mice. Results: 1) FGF-2 treatment reversed high glucose induced growth inhibition of EPCs. FGF-2 treatment also increased migration and tube formation ability of EPCs even in high glucose conditions. 2) Western blot analysis demonstrated that the percentage of activated β-catenin/total β-catenin in the high glucose group were significantly lower than that in the control group, while FGF-2 treatment reversed high glucose induced β-catenin inhibition. 3) In vivo experiments demonstrated that the blood flow recovery in ischemic hind limbs of mice was significantly improved after FGF-2 treatment. Conclusion: Exogenous FGF-2 could play a role in the functional repair of damaged EPC exposed to high glucose conditions, via the activation of the Wnt/β-catenin signaling pathway. | Yang Li Xue Li Shilong Han Weishuai Lian Jie Cheng Xiaoyun Xie Maoquan Li | 2018 | Journal of Interventional Medicine2018，1，1： | 0 |
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