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| 1 | Insights into the mechanism of CXCL12-mediated signaling in trophoblast functions and placental angiogenesis显示文摘chemokine CXCL12 和它的受体 CXCR4 是重要的表明为人的胚囊培植和怀孕的前进要求的部件。成长证据显示 CXCL12/CXCR4 轴能调整 trophoblast 功能和改变的子宫的螺线动脉,它在胎座式和胎儿的结果起一个基本作用。孤儿受体 CXCR7 也被相信部分调整 CXCL12/CXCR4 轴的功能。另外, CXCL12/CXCR4/CXCR7 轴能提高在象子宫的自然漂亮房间那样的 trophoblasts 和蜕膜的房间和涉及 trophoblast 区别和侵略和胎盘的 angiogenesis 的规定的蜕膜的 stromal 房间之间的串音。另外,最近的研究证明那 CXCL12 表情与 preeclampsia 在胎盘和怀孕女人的中间三个月的羊膜的液体被提高,暗示 CXCL12 的 dysregulation 起在 preeclampsia 的致病的一个作用。在 trophoblast 功能和胎盘的 angiogenesis 的调停 CXCL12 的发信号的规章的机制的进一步的理解可以帮助为联系怀孕的疾病设计新奇治疗学的途径。 | Liang Wang Xueyi Li Yilin Zhao Chao Fang Yingli Lian Wenli Gou Tao Han Xiaoming Zhu | 2015 | Acta Biochimica et Biophysica Sinica2015，47，9： | 8 |
| 2 | The investigation for the relationship among serum leptin, erythrocyte membrane Ca^(2+)-ATPase activity and hypertensive disorder complicating pregnancy显示文摘Objective: To study the significance of Leptin and the activity of erythrocyte membrane Ca2+-ATPase(EMCA) in the development of hypertensive disorder complicating pregnancy. Methods: Radioimmunoassay was used to test the level of serum Leptin,and the activity of EMCA was determined chemically in 38 pregnant women with hypertensive disorder complicating pregnancy and 36 normotensive pregnant women. Results: The level of serum Leptin in hypertensive disorder complicating pregnancy(gestational hypertension: 13.76 ± 3.46 ng/ml; preeclampsia:15.76 ± 5.47 ng/ml; eclampsia: 18.32 ± 6.38 ng/ml)was significantly higher than that in normotensive pregnant women(11.33 ± 2.93 ng/ml),respectively. The average EMCA activity of patients with hypertensive disorder complicating pregnancy(gestational hypertension:1.65 ± 0.24 μmol·pi/mg·h ; preeclampsia:1.37 ± 0.19 μ mol·pi/mg·h; eclampsia:1.12 ± 0.14 μ mol·pi/mg·h) was significantly lower than that of normotensive pregnant women(1.83 ± 0.38 μ mol·pi/mg·h),respectively. There was a negative correlation between the level of serum Leptin and the activity of RMCA in hypertensive disorder complicating pregnancy(r = -0.63). Conclusion: Inhibition of EMCA activity of erythrocyte in hypertensive disorder complicating pregnancy may increase cytoplasmic free calcium, which contributes to the development of hypertensive disorder complicating pregnancy. The negative correlation between the level of serum Leptin and the activity of EMCA, also suggested that serum Leptin and the activity of EMCA may play a role in the development of hypertensive disorder complicating pregnancy. | Chunfang Li Wenli Gou Xuelian Chen Shuping Zhang | 2007 | Journal of Nanjing Medical University2007，21，3： | 4 |
| 3 | Effect of artesunate on human endometrial carcinoma HEC-1B cells显示文摘Objective:To observe the effect of the artesunate(ART) on cellular proliferation in vitro,to search for the possible anti-tumor mechanism of ART on endometrial carcinoma at the molecular level and to provide the experimental and theoretical foundations for the clinical applications of ART.Methods:The cell proliferation was observed by microscope;MTT was used to examine the effects of ART on proliferation of HEC-1B cells,and flow cytometric analysis was used to detect cell cycle and apoptosis.The human endometrial carcinoma HEC-1B cells were conventionally cultured;ART was administered with a concentration of 40 μg/ml before the total RNA were extracted.mRNA expression of Survivin,Caspase-3,N-Cadherin,E-Cadherin,Fibronectin1 and Cox-2 were detected using RT-PCR.Results:ART reduced proliferation in human endometrial carcinoma cell line HEC-1B in a dose-and time-dependent effect.The cells of G0/G1 stage were significantly increased(P<0.05),but the cells of G2/M stages were significantly decreased(P<0.05),so it has shown that the cell cycle was probably blocked in G0/G1 stage.After intervention with ART at 20 and 80 μg/ml for 48 h,cellular apoptosis rate respectively was(36.42±0.77)% and(11.77±0.58)%,and the difference was statistically significant compared with the control([6.64±0.19]%,P<0.01).The expression of Cox-2 mRNA in the ART group was lower than those of control group,yet the expression of Caspase-3 and E-Cadherin mRNA in the ART group was higher than those of control group.Conclusion:ART can inhibit HEC-1B cell growth and proliferation in a dose-and time-dependent manner.Furthermore,ART can induce apoptosis in a dose-dependent manner.ART is able to downregulate Cox-2 mRNA expression and to upregulate E-Cadherin and Caspase-3 mRNA expression.So we can conclude that ART could induce the endometrial carcinoma HEC-1B cell apoptosis and inhibit tumor cell proliferation. | Wang Lijuan Yang Yucong Gou Wenli | 2010 | Journal of Medical Colleges of PLA(China)2010，25，3： | 2 |
| 4 | Effect of selective uterine artery embolization on symptomatic uterine fibroids显示文摘Objective: To evaluate the outcomes of selective uterine artery embolization (SUAE) in treatment of symptomatic uterine fibroids using Color Doppler ultrasonography (CDUS). Methods: As the procedure SUAE for fibroids, prospective data of the initial 65 consecutive women treated from March 2007 to September 2009 were collected. The follow-up period from 1 week to 30 months, using questionnaires, we investigated the fibroid-related physical and psychological symptoms, and the cumulative rates of symptom control, gynecologic interventions, and complications. The volumes of uterine and uterine fibroids were calculated by CDUS pre- and post-SUAE for 6 months. And we tested the levels of internal hormone including FSH, LH and E2 before and after SUAE for 3, 6 months. Results: SUAE was performed successfully in all patients. There was no peri-operative morbidity in all procedures. Compared with pre-SUAE volumes of uterine [(322±112) cm3] and uterine fibroids [(125±46) cm3], their volumes in post-SUAE were (144±72) cm3 and (51±11) cm3 (P<0.01), and reduction rate was 58% and 61%, respectively. At 3, 6 months after SUAE, levels of FSH, LH, and E2 were not decreased than that of in pre-SUAE (P>0.05). After SUAE for 24 months, the fibroids related physical and psychological symptoms such as bleeding, pain and quality of life were significantly improved (P<0.01). 90.8% (59/65) women had resolution of symptoms and no significant post-procedural symptoms. Parts of patients discharge necrotic fibroids. 86% (34/40) complications were minor, requiring no therapy. 9.2% (6/65) had significant post-procedural symptoms, requiring therapy in hospital or out-patient department. Conclusion: SUAE contributes to a long-term significant improvement of all investigated uterine fibroid-related symptoms and markedly improves women's health-related quality of life. SUAE that no impair on changes of internal hormone, under suitable conditions, represents an attractive alternative to hysterectomy for the treatment of symptomatic fibroids and is a minimally invasive, new method of good clinical prognosis. CDUS is an effective to investigate the outcome of SUAE. | Feng Jun Liu Yamin Zhang Yushun Wang Lijuan Wang Xingye Qin Hao Gou Wenli | 2009 | Journal of Medical Colleges of PLA(China)2009，24，6： | 1 |
| 5 | Effect of behavior training on learning and memory of young rats with fetal growth restriction显示文摘Objective: To investigate the effect of behavior training on the learning and memory of young rats with fetal growth restriction (FGR). Methods: The model of FGR was established by passive smoking method to pregnant rats. The new-born rats were divided into FGR group and normal group, and then randomly subdivided into trained and untrained group respectively. Morris water maze behavior training was performed on postnatal months 2 and 4, then learning and memory abilities of young rats were measured by dark-avoidance testing and step-down testing. Results: In the dark-avoidance and step-down testing, the young rats’ performance of FGR group was worse than that of control group, and the trained group was better than the untrained group significantly. Conclusion: FGR young rats have descended learning and memory abilities. Behavior training could improve the young rats’ learning and memory abilities, especially for the FGR young rats. | Li Xuelan Gou Wenli Huang Pu Li Chunfang Sun Yunping | 2008 | Journal of Medical Colleges of PLA(China)2008，23，5： | 0 |
| 6 | Effect of behavior training on learning,memory and the expression of NR2B,GluR1 in hippocampus of rats offspring with fetal growth restriction显示文摘Objective:To study effects of behavior training on learning,memory and the expression of NR2B,GluR1 in hippocampus of rat's offspring with fetal growth restriction(FGR).Methods:The rat model of FGR was established by passive smoking method.The rats offspring were divided into the FGR group and the control group,then randomly divided into the trained and untrained group,respectively. Morris water maze test was proceeded on postnatal month(PM2/4)as a behavior training method,then the learning-memory of rats was detected through dark-avoidance and step-down tests.The expressions of NR2B and GluR1 subunits in hippocampal CA1 and CA3 areas were detected by immunohistochemical method.Results:In the dark-avoidance and step-down tests,the performance record of rats with FGR was worse than that of control rats,and the behavior-trained rats was better than the untrained rats,when the FGR model and training factors were analyzed singly.The model factor and training factor had significant interaction(P<0.05).The expressions of NR2B and GluR1 subunits in hippocampal CA1 and CA3 areas of rats with FGR reduced.In contrast,the expressions of GluR1 and NR2B subunits in CA1 area of behavior-trained rats increased,when the FGR model and training factors were analyzed singly. Conclusion:These findings suggested that the effect of behavior training on the expressions of NR2B and GluR1 subunits in CA1 area should be the mechanistic basis for the training-induced improvement in learning-memory abilities. | Chunfang Li Wenli Gou Yunping Sun Huang Pu | 2008 | Journal of Nanjing Medical University2008，22，5： | 0 |
| 7 | STUDY ON THE PLATELET INTRACELLULAR FREE Ca^(2+) IN NORMAL PREGNANCY显示文摘This study measured platelet intracellular free calcium (PFCa 2+ ) of 1003 normal pregnancies in different trimester. The purpose of this study was to find the alteration of PFCa 2+ in normal pregnancy, to provide scientific basis for forecasting and preventing PIH (pregnancy induced hypertension). The results showed that the PFCa 2+ concentration was stable and slightly increased with the progression of gestational weeks. | Li Xuelan,Gou Wenli,Liu Guocheng,Liu Hongli Department of Obstetrics Gynecology, First Affiliated Hospital, Xi′an Medical University, Xi′an 710061 | 1999 | Journal of Pharmaceutical Analysis1999，13，2： | 0 |
| 8 | Microcarriers promote the through interface movement of mouse trophoblast stem cells by regulating stiffness显示文摘Mechanical force is crucial in the whole process of embryonic development.However,the role of trophoblast mechanics during embryo implantation has rarely been studied.In this study,we constructed a model to explore the effect of stiffness changes in mouse trophoblast stem cells(mTSCs)on implantation:microcarrier was prepared by sodium alginate using a droplet microfluidics system,and mTSCs were attached to the microcarrier surface with laminin modifications,called T(micro).Compared with the spheroid,formed by the self-assembly of mTSCs(T(sph)),we could regulate the stiffness of the microcarrier,making the Young’s modulus of mTSCs(367.70±79.81 Pa)similar to that of the blastocyst trophoblast ectoderm(432.49±151.90 Pa).Moreover,T(micro)contributes to improve the adhesion rate,expansion area and invasion depth of mTSCs.Further,T(micro)was highly expressed in tissue migration-related genes due to the activation of the Rho-associated coiled-coil containing protein kinase(ROCK)pathway at relatively similar modulus of trophoblast.Overall,our study explores the embryo implantation process with a new perspective,and provides theoretical support for understanding the effect of mechanics on embryo implantation. | Zili Gao Jia Guo Bo Gou Zhen Gu Tan Jia Sinan Ma Liyuan Jiang Wenli Liu Lixun Zhou Qi Gu | 2023 | Bioactive Materials2023，，10： | 0 |
| 9 | Effects of docetaxel,oxaliplatin and their combination on HeLa strain of cervical cancer显示文摘Objective:To study the inhibitory effect of docetaxel(DOC),oxaliplatin(OXA) and their combination on proliferation of cervical cancer line HeLa.Methods:Cell morphological changes were observed by inverted phase contrast microscope,cell inhibition rates in different groups were examined by MTT,and cell cycle and apoptosis rates were determined by flow cytometry(FCM).Results:DOC and OXA inhibited the proliferation of cervical cancer cell line HeLa in a dose-dependent,and combination of the two drugs had an enhanced inhibitory effect;the apoptosis rate was also significantly increased when the two drugs were used in combination.Conclusion:DOC and OXA can synergistically inhibit the proliferation of cervical cancer cell line HeLa,which indicates that combination of the two drugs might has a promising future for clinical treatment of cervical cancer. | Zou Yuliang Gou Wenli Pei Meili Xiao Jinghua Liu Xiaolian | 2010 | Journal of Medical Colleges of PLA(China)2010，25，4： | 0 |
| 10 | Impairments of spatial learning and memory in rat offspring with fetal growth restriction显示文摘Objective:Throughout the world,fetal growth restriction(FGR) is one of the most severe complications occurring during pregnancy. It is subsequently associated with neurologic abnormalities in chldren. Our aim was to investigate the spatial learning and memory ability of rat offspring born with FGR. Methods:A rat model of FGR was constructed using the method of passive smoking. Spatial learning and memory were studied in rat offspring born with FGR by assessing the animals’ performance using the Morris water maze task. Results: At 1-and 2-months of age,both female and male offspring rats showed impairment of performance,while at 4 months of age,only female rats showed impaired performance. The FGR offspring spent a longer time swimming and used inefficient strategies(P < 0.05,respectively). However,there were no significant maze performance FGR effects in the 4 month old male rats. In all groups of FGR offspring,irrespective of age or sex,the time spent in the platform quadrant by the rat was significantly less than that in the control group(P < 0.05). Conclusion: The Morris water maze performance decreased in rat offspring born with FGR. It is suggested that FGR can cause impairments of spatial learning and memory in young animals. | Pu Huang Wenli Gou Mali Jiang Rui Zhang Yunping Sun | 2009 | Journal of Nanjing Medical University2009，23，1： | 0 |
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