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| 1 | Evodiamine induces extrinsic and intrinsic apoptosis of ovarian cancer cells via the mitogen-activated protein kinase/phosphatidylinositol-3-kinase/protein kinase B signaling pathways显示文摘OBJECTIVE: To explore the effects of evodiamine on ovarian cancer cells and the mechanisms underlying such effects.METHODS: Human ovarian cancer cells HO-8910 PM were treated with evodiamine at 0, 1.25,2.5, and 5 μM for 1-4 d. 3-(4,5-Dimethiylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) assay was used to detect the growth inhibition rate of evodiamine-treated HO-8910 PM cells. The cell cycle was observed via propidium iodide(PI) staining. Apoptosis induction was assessed via Annexin V-fluorescein isothiocyanate/propidium iodide(Annexin V-FITC/PI) double staining assay. To verify the mechanism of apoptosis, caspase-dependent apoptotic pathway-related protein was detected by Western blot analysis. The expression levels of mitogen-activated protein kinase(MAPK)and/or phosphatidylinositol-3-kinase(PI3K)/pro-tein kinase B(Akt) pathway-related proteins were also investigated.RESULTS: Evodiamine significantly inhibited the proliferation of HO-8910 PM cells in a dose- and time-dependent manner. Evodiamine induced G2/M arrest with an increase of cyclin B1 level, and promoted cell apoptosis with a decrease of B cell lymphoma/lewkmia-2(Bcl-2) and an increase of Bcl-2-associated X protein(Bax) level. In addition,evodiamine treatment led to the activation of caspase-8, caspase-9, and caspase-3 and the cleavage of poly(ADP-ribose)-polymerase(PARP). Evodiamine targeted the MAPK and/or PI3K/Akt pathways by reducing the expression and activity of PI3 K, Akt, and extracellular signal-regulated kinase mitogen-activated protein kinase(ERK1/2 MAPK)and the activity of p38 MAPK.CONCLUSION: Evodiamine can inhibit the growth of ovarian cancer cells by G2/M arrest and intrinsic and extrinsic apoptosis. In addition, evodiamine-induced PI3K/Akt, ERK1/2 MAPK, and p38 MAPK signaling may be involved in cell death. | Wei Lijuan Jin Xiaoying Cao Zipei Li Wenlu | 2016 | Journal of Traditional Chinese Medicine2016，36，3： | 5 |
| 2 | The pattern of plasma BCAA concentration and liver Bckdha gene expression in GK rats during T2D progression显示文摘Background: This study was conducted to measure the concentration of branched chain amino acid(BCAA) in different species and detect the expression pattern of the liver Bckdha gene in Goto-Kakizaki(GK) rats during type 2 diabetes(T2D)progression.Methods: We measured the concentration of BCAA in GK rats, induced T2D cynomolgus monkeys and T2D humans by liquid chromatography tandem mass spectrometry, and used real-time quantitative PCR to analyze the gene expression of Bckdha and Bckdk, which encode the rate-limiting enzymes in catabolism of,respectively, branched chain amino acids and branched chain α-keto acid dehydrogenase kinase.Results: In this study, we showed that GK rat BCAA concentrations were significantly reduced at 4 and 8 weeks(P < 0.05 and P < 0.01, respectively), while the expression of Bckdha in GK rat liver was increased at 4 and 8 weeks(1.62-fold and1.93-fold, respectively). The BCAA concentrations were significantly reduced in diet-induced T2D cynomolgus monkeys(P < 0.01), but significantly increased in T2D humans(P < 0.001).Conclusions: Our results showed that BCAA concentrations changed at different times and by different amounts in different species and during different periods of T2D progress, and the significant changes of BCAA concentration in the three species indicated that BCAA might participate in the progress of T2D. The results suggested that the increased expression of Bckdha in GK rat liver might partially explain the reduced plasma BCAA concentration at 4 and 8 weeks. Further studies are required to investigate the exact mechanism of BCAA changes in non-obese T2D. | Wenlu Zhang Yu'e Wu Wei Fan Hongmei Chen Hongli Du Junhua Rao | 2018 | Animal Models and Experimental Medicine2018，1，4： | 1 |
| 3 | Strategies to improve the fluorescent signal of the tripartite sfGFP system显示文摘Bimolecular fluoresce nee compleme ntati on(BiFC)is a popular method used to detect proteinprotein interactions.For a BiFC assay,a fluorescent protein is usually split into two parts,and the fluoresce nee is recovered upon the in teracti on between the fused proteins of interest.As an elega nt extensi on of BiFC,a tripartite superfold green fluoresce nt protein(sfGFP)system that has the advantages of low background fluorescence and small fusion tag size has been developed.However,the tripartite system exhibits a low fluorescence signal in some cases.To address this problem,we proposed to in crease the affinity betwee n the two parts,G1-9 and G11,of the tripartite system by adding affinity pairs.Among the three affinity pairs tested,LgBiT-HiBiT improved both the signal and sign a I-to-noise(S/N)ratio to the greatest exte nt.More strikingly,the direct covalent fusion of G11 to G1-9,which converted the tripartite system into a new bipartite system,enhanced the S/N ratio from 20 to 146,which is superior to the bipartite sfGFP system split at 157/158 or 173/174.Our results implied that the 10th p-strand of sfGFP has a low affinity and a good recovery efficiency to construct a robust BiFC system,and this concept might be applied to other fluorescent proteins with similar structure to construct new BiFC systems. | Jing Shen Wenlu Zhang Chunyang Gan Xiafei Wei Jie Li Yuxue Sun Yi Yuan Xuefei Cai Quanxin Long Jing Cui Haitao Guo Ailong Huang Jieli Hu | 2020 | Acta Biochimica et Biophysica Sinica2020，52，9： | 0 |
| 4 | Analysis of the different locomotive wireless multi-traction synchronous control systems for heavy-haul combined trains and their performance显示文摘This paper looks at the heavy-haul combined train composed of different types of locomotives and its distributed power control system with a combination of 800 MHz and TD-LTE wireless communication.It analyses some key characteristic parameters that affect the synchronism and communication of the differential wireless multi-traction synchronous control systems for heavy-haul combined trains.At the same time,in order to reduce the latency of instruction and information transfer between different types of locomotives,improve the time-limit certainty of wireless transmission and optimize the control quality of multi-traction control systems for heavy-haul combined trains,a synchronism optimization strategy based on the Markov decision process on the basis of Petri networkconstruction is proposed.Relevant experiments and tests are carried out to verify the effectiveness of the synchronism optimization of the control system,which provides a guarantee for improving the differential wireless multi-traction synchronous control system for combined trains and optimizing train control. | Wei Li Chunyang Chen Hui Li KaiWang Liang Xie Wenlu Zhang | 2020 | Transportation Safety and Environment2020，2，3： | 0 |
| 5 | Laser measurement system for acrylic transmittance of JUNO central detector显示文摘Background During the research phase of acrylic panel for the Jiangmen Underground Neutrino Observatory(JUNO)central detector(CD),the darkroom transmittance measurement system(DTMS)was designed and built,and a massive amount of measurements were carried out to help determine the composition and processing techniques of the acrylic panel.Motivation The mass production of acrylic panels was started after the pilot production.The requirement of transmittance measurement put forward new demands on operation,and a new measurement system was considered to support removable and non-destructive measurement on site.Result After the accomplishment of design,development and verification were completed,the laser measurement system came into use in the acrylic workshop of Taixing Donchamp Acrylic Ltd,and the transmittance results of acrylic spherical panels met the requirement of JUNO.The optical design,tooling design,data processing interface and uncertainty analyses of the laser measurement system are discussed in detail,and the measured transmittance results are also introduced in this article. | Zhaohan Li Xiaoyu Yang Yuekun Heng Xiaoyan Ma Xiaohui Qian Kaixi Huang Zhi Wu Wei He Meihang Xu Xiaolan Luo Yatian Pei Mengzhao Li Zhiyan Cai Wenlu Wei Yifang Wang | 2021 | Radiation Detection Technology and Methods2021，5，3： | 0 |
| 6 | Experimental study of channel electron multiplier显示文摘Background Channel electron multiplier(CEM)can be used to measure extremely few charged particles,such as electrons and ions.The CEM is widely used in particle detection,so it is very important to study their performance parameters.Purposes Test and analyze the performance parameters of the CEM,such as resistance,gain,and pulse output for a single entrance photoelectron.Methods The heated tantalum filament is used as a stable and adjustable planar electron source to test the performance of the CEM in the analog mode.The performance parameters of the CEM in the pulse counting mode are tested by using the ultraviolet LED to excite the gold photocathode to generate a single photoelectron.Results and conclusions The gain of the CEM in the analog mode can reach more than 106 and the gain in the pulse counting mode can be two orders of magnitude higher.The curved helical channel has a greater advantage than the ordinary straight channel,which is conducive to weakening the ion feedback phenomenon. | Binting Zhang Cuiping Li Shulin Liu Baojun Yan Baoqiang Li Wenlu Wei Huaxing Peng | 2023 | Radiation Detection Technology and Methods2023，7，1： | 0 |
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