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| 1 | A detailed procedure for CRISPR/Cas9-mediated gene editing in Arabidopsis thaliana显示文摘The newly developed CRISPR(Clustered Regularly Interspaced Short Palindromic Repeats)/Cas(CRISPR-associated) system has emerged as an efficient tool for genome-editing, providing an alternative to classical mutagenesis and transgenic methods to study gene function and improve crop traits. CRISPR/Cas facilitates targeted gene editing through RNA-guided DNA cleavage followed by cellular DNA repair mechanisms that introduce sequence changes at the site of cleavage. Here we describe a detailed procedure for our previously developed and highly efficient CRISPR/Cas9 method that allows the generation of heritable-targeted gene mutations andcorrections in Arabidopsis. This protocol describes the strategies and steps for the selection of targets, design of single-guide RNA(sg RNA), vector construction and analysis of transgenic lines. We also offer a method to target two loci simultaneously using vectors containing two different sg RNAs. The principles described in this protocol can be applied to other plant species to generate stably inherited DNA modifications. | Wenshan Liu Xiaohong Zhu Mingguang Lei Qingyou Xia Jose Ramon Botella Jian-Kang Zhu Yanfei Mao | 2015 | Science Bulletin2015，60，15： | 18 |
| 2 | Spinel + quartz-bearing ultrahigh-temperature granulites from Xumayao,Inner Mongolia Suture Zone,North China Craton:Petrology,phase equilibria and counterclockwise p-T path显示文摘在在北方中国 Craton 的内部蒙古缝术地区(IMSZ ) 以内的 Khondalite 带是为 Paleoproterozoic 的一个经典例子超离频温度(UHT ) 变态。这里,我们在 IMSZ 的南部的领域以内在 Xumayao 从一个新地区报导新忍受尖晶石的 metapelitic granulites。Petrological 研究并且尖晶石 + 的热力学的建模忍受石英的集合证明这些岩石以 UHT 条件经历了极端变态。尖晶石组织上发生在二背景:1 ) 高 XZn (Zn/(Mg +FeII + Zn )= 0.0710.232 ) 有在岩石矩阵的 perthitic K 长石,矽线石和石英的尖晶石;并且 2 ) 低 XZn (0.0450.070 ) 尖晶石作为在与石英和矽线石联合的石榴石 porphyroblasts 以内的包括。我们当模特儿的阶段 equilibria 在这些岩石的变形进化期间显示二个主要阶段: 1 )从 975 的在等压附近的冷却????畘慭慹?????????????朠慲畮楬整????????????????慲潴????????煥極楬牢慩?????????????胴?? | Huatian Zhang Jianghai Li Shoujie Liu Wenshan Li M. Santosh Honghao Wang | 2012 | Geoscience Frontiers2012，3，5： | 8 |
| 3 | Critical function of DNA methyltransferase 1 in tomato development and regulation of the DNA methylome and transcriptome显示文摘DNA methylation confers epigenetic regulation on gene expression and thereby on various biological processes.Tomato has emerged as an excellent system to study the function of DNA methylation in plant development.To date,regulation and function of DNA methylation maintenance remains unclear in tomato plants.Here,we report the critical function of tomato(Solanum lycopersicum)Methyltransferase 1(Sl MET1)in plant development and DNA methylome and transcriptome regulation.Using CRISPR-Cas9 gene editing,we generated slmet1 mutants and observed severe developmental defects with a frame-shift mutation,including small and curly leaves,defective inflorescence,and parthenocarpy.In leaf tissues,mutations in Sl MET1 caused CG hypomethylation and CHH hypermethylationon a whole-genome scale,leading to a disturbed transcriptome including ectopic expression of many RIN target genes such as ACC2 in leaf tissues,which are normally expressed in fruits.Neither the CG hypomethylation nor CHH hypermethylation in the slmet1 mutants is related to tissue culture.Meanwhile,tissue culture induces non-CG hypomethylation,which occurs more frequently at gene regions than at TE regions.Our results depict Sl MET1-and tissue culture-dependent tomato DNA methylomes,and that Sl MET1 is required for maintaining a normal transcriptome and normal development of tomato. | Yu Yang Kai Tang Tatsiana U Datsenka Wenshan Liu Suhui Lv Zhaobo Lang Xingang Wang Jinghui Gao Wei Wang Wenfeng Nie Zhaoqing Chu Heng Zhang Avtar K HANDa Jian-Kang Zhu Huiming Zhang | 2019 | Journal of Integrative Plant Biology2019，61，12： | 3 |
| 4 | 北斗卫星自主完好性监测及在轨性能评估显示文摘With the development of satellite navigation technology,the user demands for the integrity of Global Navigation Satellite System(GNSS)have increased more and more.A ground-based monitoring system can hardly report an alarm message to GNSS users during the valid alarming period due to the satellite-Earth propagation delay.It is beneficial to monitor abnormal events and report the corresponding alarms from orbit.Adopting this approach,which is an important feature for future GNSS integrity monitoring,the time needed to provide an alarm is shorter and the system integrity capability is strengthened.The BeiDou Navigation Satellite System(BDS)new generation satellites have the capabilities of satellite autonomous integrity monitoring(SAIM).This paper presents the technical scheme of SAIM,and proposes the integrity monitoring method of both navigation signals and the clocks onboard.The proposed method was verified through the onboard test on the BDS satellites.In addition,we analyzed the integrity telemetry data from the new generation of BDS satellite,including signal delay,power,carrier phase measurement,correlation peak,consistency of pseudo-code and carrier phase,clock phase and frequency step.The analysis results indicated that the quality of the data on orbit met the requirements,and SAIM could monitor effectively any abnormal change of satellite clocks and navigation signal,generate rapidly an alarm message,and transmit it to the user.The alarm time was less than 6 s through the message,and 2 s through non-standard code(NSC).Finally,we present future opportunities for improving the SAIM technology of BDS. | BIAN Lang LIU Xiao LIU Wenshan YAN Tao LEI Wenying JIA Yizhe MENG Yansong ZHANG Lixin | 2020 | Aerospace China2020，21，4： | 2 |
| 5 | 拟南芥ABA受体PYL的十二重突变体揭示了PYL对ABA不依赖的渗透胁迫应答的抑制显示文摘文章简介ABA促进气孔关闭、生长抑制、休眠等胁迫应答,被称为'胁迫激素'。除胁迫应答之外,ABA信号是否参与调控其他生理过程尚不清楚。本研究构建了敲除所有ABA受体的PYL的十四重突变体,发现它极矮小且不育. | 赵杨 Zhengjing Zhang 高景慧 王鹏程 胡涛 Zegang Wang Yueh-Ju Hou Yizhen Wan Wenshan Liu Shaojun Xie Tianjiao Lu Liang Xue Yajie Liu Alberto P.Macho W.Andy Tao Ray A.Bressan 朱健康 | 2019 | 科学新闻2019，0，2： | 1 |
| 6 | A study on the Xizang(Tibet) salt minerals[J]显示文摘 | Zheng Mianpin Liu Wengao Jin Wenshan | 1981 | 中国地质科学院院报-矿产地质研究所专刊1981，2，1： | 1 |
| 7 | Rearing venus clam seed, cyclina sinensis (Gmelin), on a commercial scale显示文摘 | Liu Wenshan Ma Yuehua Hu Shouyi | 2002 | Aquaculture2002，211，： | 1 |
| 8 | Rearing Venus clam seeds, Cyclina sinensis (Gmelin), on a commercial scale 显示文摘 | Wenshan Liu Yuehua Ma Shouyi Hu | 2002 | Aquaculture2002，211，： | 1 |
| 9 | Networked predictive control of systems with random network delays in both forward and feedback channels显示文摘 | GUO-PING LIU YUANQING XIA JIE CHEN DAVID REES AND WENSHAN HU | 2007 | IEEE transactions on industrial electronics2007，54，3： | 1 |
| 10 | Rearing venus clamseeds, Cyelina sinensis(Gmelin), on a commercial scale显示文摘 | Liu Wenshan Ma Yuehua Hu Shouyi | 2002 | Aquaculture2002，211，4： | 1 |
| 11 | Gradient-based method for the identification of multi-nodes in sugarcane显示文摘In view of the obvious changes in color between the upper and lower leaf scar in sugarcane nodes,a method of simultaneous multi-nodes identification on a single sugarcane stem was proposed based on the analysis of gradient characteristics of sugarcane images.In combination with image processing and machine vision recognition technology,two cameras were used to acquire different parts of sugarcane images,and the two images were integrated into a complete image of sugarcane by image mosaicking.The Sobel operator is used to calculate the gradient of the sugarcane image in a horizontal direction,and the gradient image is obtained.The sugarcane gradient image was scanned by a rectangular template with a width of 14 pixels and a step length of 12 pixels.The features of average gradient and variance gradient were used to identify sugarcane nodes for the first time.The experimental results showed that the recognition accuracy was 96.8952%,and there were fewer false detected sugarcane segments.The detection efficiency could be improved by detecting multi-nodes on a single sugarcane stem at the same time. | Rui Yang Jie Li Qi Liu Wenshan Huang Kai Yin Xi Qiao Yiqi Huang | 2020 | Information Processing in Agriculture2020，7，4： | 1 |
| 12 | Design and Stability Criteria of Networked Predictive Control Systems with Random Network Delay in the Feedback Channel显示文摘 | Guo-Ping Liu Yuanqing Xia David Rees and Wenshan Hu | 2007 | IEEE Transactions on System Man and Cybernetics-part c: Applications and Reviews2007，37，2： | 1 |
| 13 | Decontamination Efficiency of Slightly Acidic Electrolyzed Water on Fresh-Cut Cucumbers显示文摘 | Zhuqing Liu Yu Dong Wenshan Jiang | 2011 | International Journal of Food Engineering2011，，5： | 1 |
| 14 | Rearing venus clam seeds, Cyclina sinensis (Gmelin), on a commercial scale 显示文摘 | Wenshan Liu Yuehua Ma Shouyi Hu | 2002 | Aquaculture2002，211，： | 1 |
| 15 | Rearing venus clam seeds, Cyclina sinensis (Gmelin), on a commercial scale显示文摘 | LIU Wenshan MA Yuehua HU Shouyi | 2002 | Aquaculture2002，211，： | 1 |
| 16 | Web-based 3D Control Laboratory for Remote Real Time Experimenta- tion 显示文摘 | Hu Wenshan Liu Guoping Zhou Hong | 2013 | IEEE Transactions on Industrial Electronics2013，60，10： | 1 |
| 17 | The role of TNF-alpha/NF-kappa B pathway on the up-regulation of voltage-gated sodium channel Nav1.7 in DRG neurons of rats with diabetic neuropathy显示文摘 | Yangliang Huang Ying Zang Lijun Zhou Wenshan Gui Xianguo Liu Yi Zhong | 2014 | Neurochemistry International2014，，： | 1 |
| 18 | Web-based 3D control laboratory for remote real time experimentation显示文摘 | Hu Wenshan Liu Guoping Zhou ttong | 2013 | lndustrial Electronics IEEE Transactions on2013，60，10： | 1 |
| 19 | A simple and sensitive assay for apurinic/apyrimidinic endonuclease 1activity based on host-guest interaction of β-cyclodextrin polymer and pyrene显示文摘Apurinic/apyrimidinic endonuclease 1(APE1) plays an important role in protecting the fidelity of genetic material and is also a promising biomarker of cancers. Herein, a simple and sensitive fluorescent method for APE1 activity detection was designed based on host-guest interaction between β-cyclodextrin polymer(β-CDP) and pyrene. In this method, pyrene-labelled DNA probes(AP-S1 S2) and β-CDP functioned as fluorescent signal producer and enhancer, respectively. When APE1 was absent, pyrene on the AP-S1 S2 could not enter the cavity of β-CDP because of steric hindrance, leading to a weak fluorescence intensity/anisotropy. When APE1 was present, it would recognize and cleave AP site in APS1 S2, thus producing a dissociated pyrene-labelled oligonucleotide segment. Pyrene labelled at the 50 end of oligonucleotide segment could be easily trapped into the cavity of β-CDP via host-guest interaction, leading to a significant enhancement of fluorescence intensity/anisotropy. This assay offered a dynamic range of 0.05 U/m L–5 U/m L and an estimated detection limit of 0.05 U/m L. Furthermore,assessment of APE1 activity in He La cell extracts was successfully implemented, which hold the potential for APE1-related cancer diagnosis and biological researches. | Maogui Zhou Shiya Qin Zhenzhen Feng Chunxia Song Hua Zhang Wenshan Li Qing Wang Jianbo Liu Jin Huang Xiaohai Yang Kemin Wang | 2018 | Chinese Chemical Letters2018，29，6： | 0 |
| 20 | Effects of frost action on soil physical properties of plough pan显示文摘Undisturb ed soil cores of loamy clay plough pan were subjected to the treatments of freezing and thawing in laboratory. The effects of frost action on its bulk density, porosity, and hydraulic conductivity were investigated. The results indicate that freezing and thawing significantly reduced the bulk density of plough pan and increased its porosity and hydraulic conductivity. The reduction in the bulk density was closely related to the average daily soil minimum temperature and soil water content. | Ximin Deng Jian Wang Wenshan Zhu Duansheng Chen Liping Liu | 1999 | Chinese Science Bulletin1999，44，5： | 0 |
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