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| 1 | Genomics and genetic breeding in aquatic animals:progress and prospects显示文摘Genomics focuses on dissection of genome structure and function to provide a molecular basis for understanding the genetic background. In a pivotal step,the expense of whole genome sequencing has been largely eliminated by the rapid updating of sequencing technology,leading to increasing numbers of decoded genomes of aquatic organisms, driving the aquaculture industry into the genomic era. Multiple aquatic areas have been influenced by these findings, such as accelerated generation shift in the seed industry and the process of breeding improved lines. In this article, we have summarized the latest domestic and international progress of aquatic animals in nine aspects, including WGS and fine mapping,construction of high density genetic/physical maps, traitrelated marker/genes screening, as well as sex control,genome editing, and other molecular breeding technologies. Finally, the existing problems in this field have been discussed and five future counter measures have been proposed accordingly. | Wenteng XU Songlin CHEN | 2017 | Frontiers of Agricultural Science and Engineering2017，4，3： | 2 |
| 2 | Real-time queue length estimation for congested signalized intersections显示文摘 | Liu H X Wu Xinkai Ma Wenteng | 2009 | Transportation Research Part C:Emerging Technologies2009，17，4： | 1 |
| 3 | Immobilization of can- dida rugosa lipase on chitosan with activation of the hy- droxyl groups显示文摘 | Chiou Shaohua Wu Wenteng | 2004 | Biomaterials2004，25，2： | 1 |
| 4 | Regeneration of immobi- lized Candida antarctica lipase for transesterification显示文摘 | CHEM Jechwei WU Wenteng | 2003 | Journal of Bioscience and Bioengioeering2003，95，： | 1 |
| 5 | Real-time queue length estimation for congested sig nalized intersections显示文摘 | Henry X Liu Xinkai Wu Wenteng Ma etc | 2009 | Transportation Research Part C:Emerging Technologies2009，17，4： | 1 |
| 6 | Real-time queue length estimation for congested signalized intersections显示文摘 | Henry X. Liu Xinkai Wu Wenteng Ma Heng Hu | 2009 | Transportation Research Part C2009，，4： | 1 |
| 7 | Functional analysis of the promoter of the dmrt1 gene in Chinese tongue sole,Cynoglossus semilaevis显示文摘The dmrt1 (doublesex and mab-3 related transcription factor 1) gene is considered to play an essential role in testis differentiation and development among metazoan species. As an economically important marine fish in China, Chinese tongue sole (Cynoglossus semilaevis) possess a ZW/ZZ sex-determining system, and the females usually grow two to four times bigger than males. Previous studies have confirmed that Z-linked dmrt1 gene is the male determining gene in C. semilaevis and TALENs-mediated knocking-out individuals showed increased growth phenotype. In order to investigate the function of dmrt1 regulatory regions and its potential application, we have cloned the promoter of C. semilaevis dmrt1. The acquired sequence consisted of a 5'-upstream sequence and a partial exon1;functional analysis showed that there were two positive regulatory regions in the promoter, as well as one negative region and one neutral region. These positive regions were then combined to obtain several newly constructed promoters. Both in vitro and in vivo analysis confirmed one of them (dmrt1-Δ3+Δ4) showed elevated promoting activity compared to the full-length promoter and could drive exogenous gene expression in tongue sole gonads. Our results will provide useful information for understanding the regulatory mechanism of dmrt1 gene during C. semilaevis sex determination, and the endogenous promoter will facilitate the transgenic research for analyzing sex- and growth-related genes. | WANG Qian CUI Zhongkai GUO Hua ZHANG Nianwei XU Wenteng YANG Yingming CHEN Songlin | 2019 | Journal of Oceanology and Limnology2019，37，4： | 1 |
| 8 | Curvature analysis of spatial cam-follower mechanisms显示文摘 | YAN Hongsen CHENG Wenteng | 1999 | Mechanism and Machine Theory1999，34，2： | 1 |
| 9 | Real-time queue length estimation for congested signalized intersections 显示文摘 | LIU X Henry WU Xinkai MA Wenteng | 2009 | Transportation Research Part C2009，17，： | 1 |
| 10 | Real-time queue length estimation for congested signalized intersections显示文摘 | Henry X. Liu Xinkai Wu Wenteng Ma Heng Hu | 2009 | Transportation Research Part C2009，，4： | 1 |
| 11 | Real-time queue length estimation for congested signalized intersections显示文摘 | Liu Henry Wu Xinkai Ma Wenteng | 2009 | Transportation Research Part C2009，17，： | 1 |
| 12 | A novel photobioreactor with transparent rectangular chambers for cultivation of microalgae 显示文摘 | HSIEH C H WU Wenteng | 2009 | Biochemical Engineering Journal2009，46，： | 1 |
| 13 | QTL Detection for Albinism-Related Loci in Chinese Tongue Sole(Cynoglossus semilaevis)显示文摘The Chinese tongue sole(Cynoglossus semilaevis) is widely cultured in the coastal region of East Asia and has excellent economic value. However, the high albino rate of the breeding population has caused a significant loss to the aquaculture industry. To study the molecular mechanism of albinism, the present study used an albino Chinese tongue sole family to construct three simple sequence repeat(SSR) linkage groups, and draft a preliminary linkage map related to albinism. After albinism-related loci mapping, 18 albinism-related loci were detected under two models(containing 2407 genes) compared to the Chinese tongue sole genome. One of these loci, the tyrosinase related protein(tyrp2), which has been reported previously as an important gene regulating both eumelanin and phaeomelanin levels, was indicated to be the possible cause of albinism. Thirty-five Gene Ontology(GO) terms and 14 Kyoto Encyclopedia of Genes and Genome pathways were annotated via bioinformatic analyses. One GO term with protein tyrosine kinase activity, which contained 10 genes, was previously suggested to affect fish albinism. These results establish a foundation for further in-depth study of albinism in Chinese tongue sole. | LIU Yang XU Wenteng ZHANG Bo SHAO Changwei WANG Xiaoxia SUN Hejun WANG Na CHEN Songlin | 2018 | Journal of Ocean University of China2018，17，6： | 1 |
| 14 | Realtime queue length estimation for congested signalized intersections显示文摘 | Henry X Liu Xinkai Wu Wenteng Ma | | 0，，： | 1 |
| 15 | Real - time Queue Length Estimation for Congested Signalized Intersections 显示文摘 | LIU H X WU Xinkai MA Wenteng | 2009 | Transportation Research Part C: Technology2009，17，4： | 1 |
| 16 | Real- time queue length estimation for congested signalized intersections显示文摘 | Liu H X Wu Xinkai Ma Wenteng | 2009 | Transportation Research Part C2009，17，4： | 1 |
| 17 | Real-time estimation of arterial travel time under congested conditions显示文摘 | HenryX. Liu Wenteng Ma Xinkai Wu Heng Hu | 2012 | Transportmetrica2012，，2： | 1 |
| 18 | A virtual vehicle probe model for time-dependent travel time estimation on signalized arterials显示文摘 | Henry X. Liu Wenteng Ma | 2008 | Transportation Research Part C2008，，1： | 1 |
| 19 | Immobilization of Candida ru- gosa lipase on chitosan with activation of the hydroxyl groups 显示文摘 | CHIOU Shaohua WU Wenteng | 2004 | Biomaterials2004，25，2： | 1 |
| 20 | Real-time Queue Length Estimation for Congested Signalized Intersections 显示文摘 | LIU H X WU Xinkai MA Wenteng | 2009 | Transportation Research2009，，17： | 1 |
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