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| 1 | Effect of sucrose and potassium nitrate on biomass and saponin content of Talinum paniculatum Gaertn. hairy root in balloon-type bubble bioreactor显示文摘Objective:To increase biomass and saponin production in hairy root culture of Talinum paniculatum Gaertn.(T.paniculatum)in balloon-type bubble bioreactor(BTBB).Methods:Hairy roots which were collected from leaf explants of T.paniculatum were infected by Agrobacterium rhizogenes strain LB510.The hairy roots were cultivated at400 m L Murashige and Skoog liquid medium without growth regulator(MS0)in1 000 m L BTBB.Each BTBB had 2 g hairy roots as initial inoculum and these cultures were treated with various concentrations of sucrose(3%,4%,5%,6%w/v)and potassium nitrate(0.5,1.0,1.5 and 2.0 strength of MS medium).Cultures were maintained for 14days.Fresh and dry weights of hairy roots at the end of culture were investigated.Results:Various concentrations of sucrose influenced the biomass accumulation of hairy roots.Maximum biomass was reached by MS medium supplemented with 6%sucrose and it was approximately threefold higher than control.Culture supplemented with potassium nitrate at 2.0 strength of MS0 could increase biomass accumulation of hairy roots until 0.14 g dry weight and it was almost threefold higher than control.However,the maximum saponin content was obtained by MS medium supplemented with 5%sucrose and 2.0 strength potassium nitrate of MS.Conclusions:Based on this research,those conditions can be used to produce biomass and saponin of hairy root of T.paniculatum in the large scale. | Yosephine Sri Wulan Manuhara Alfinda Novi Kristanti Edy Setiti Wida Utami Arif Yachya | 2015 | Asian Pacific Journal of Tropical Biomedicine2015，5，12： | 3 |
| 2 | Killing of leukemic cells with a BCR/ABL fusion gene by RNA interference(RNAi)显示文摘 | Wida M Fuchs U Wossmann W | 2002 | Oncogene2002，21，37： | 1 |
| 3 | Bacterial microflora of the chicken embryos and newly hatched ehicken显示文摘 | Kizerwetter-S'wida M Binek M | 2008 | Journal of Ani- ma and Feed Sciences2008，17，4： | 1 |
| 4 | PFGE and AFLP genotyping of Staphylococcus aureus subsp . anaerobius isolated from goats with Morel’s disease显示文摘 | O. Szalu?-Jordanow D. Chrobak M. Pyrgiel A. Lutyńska J. Kaba M. Czopowicz L. Witkowski M. Kizerwetter-?wida M. Binek T. Frymus | 2013 | Archives of Microbiology2013，，1： | 1 |
| 5 | Killing ofleukemic cells with a BCR/ABL fusion gene by RNA interference(RNAi) 显示文摘 | Wida M Fuchs U Wossmann W | 2002 | Oncogene2002，2，37： | 1 |
| 6 | RISK FACTORS FOR RENAL ALLOGRAFT SURVIVAL FROM PEDIATRIC CADAVER DONORS: AN ANALYSIS OF UNITED NETWORK FOR ORGAN SHARING DATA1显示文摘 | Barbara A. Bresnahan Maureen A. McBride Wida S. Cherikh Sundaram Hariharan | 2001 | Transplantation2001，，2： | 1 |
| 7 | Maintenance Immunosuppression with Target-of-Rapamycin Inhibitors is Associated with a Reduced Incidence of De Novo Malignancies显示文摘 | H Myron Kauffman Wida S. Cherikh Yulin Cheng Douglas W. Hanto Barry D. Kahan | 2005 | Transplantation2005，，7： | 1 |
| 8 | Acceleration of ATP-induced contraction in the obstructed bladder of the rat 显示文摘 | Wida H | 2003 | Nippon Hinyokika-Gakrai Zasshi2003，94，3： | 1 |
| 9 | Risk factors for renal allograft survival from pediatric cadaver donors: an analysis of united network for organ sharing data 显示文摘 | Barbara AB Maureen MA Wida CS | 2001 | Transplantation2001，72，2： | 1 |
| 10 | In vitro propagation of the endangered medicinal orchid,Dendrobium lasianthera J.J.Sm through mature seed culture显示文摘Objective:To study asymbiotic seed germination and mass propagation of Dendrobium lasianthera which is one of the endangered medicinal orchids using seeds.Methods:The 14 weeks old hand pollinated seeds were sown on Vacin and Went(VW)solid medium supplemented with various concentrations of peptone(1,2,3 g/L) and without peptone which was used as control treatment.At the 4,8,and 12 weeks after the seeds were sown,seed germination and shoot formation were investigated.To evaluate the role of organic nutrient additives on subsequent shoot development and root formation,particular shoots with about 1 cm length contains 1–2 leaves obtained from the seeds germination was cultured on VW medium additives with different of organic nutrient:15% coconut water,2 g/L peptone,150 g/L banana homogenate,and without organic nutrient was used as control.After 16 weeks of culture,the plantlet height,number of leaves,number of roots,leaf length and root length were recorded.Results:The supplementation of 2 g/L peptone in VW medium was proven to be suitable concentration for seed germination(100%) and shoot formation with 84.0% the protocorm development to phase 5(shoot).VW medium containing 15% coconut water was effectively improved the shoot development,with well developed roots and leaves compared to the other treatment and 95% of acclimatized plantlets survived.Conclusions:This protocol is an efficient way for the in vitro mass propagation of this Dendrobium lasianthera. | Edy Setiti Wida Utami Sucipto Hariyanto Yosephine Sri Wulan Manuhara | 2017 | Asian Pacific Journal of Tropical Biomedicine2017，7，5： | 0 |
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