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| 1 | Isolation of bacteria from fermented food and grass carp intestine and their efficiencies in improving nutrient value of soybean meal in solid state fermentation显示文摘Background: Soybean meal is an excellent and cost-effective protein source; however, its usage is limited in the piglet due to the presence of anti-nutritional factors and the antigens glycinin and β-conglycinin. The objective of the current study was to screen and select for bacteria that can be efficiently adopted to ferment soybean meal in order to solve this problem.Results: Bacteria were isolated from fermented soy foods and the grass carp intestine, and strains selected for high protease, cellulase and amylase activities. The isolated bacteria were characterized as Bacillus cereus, Bacillus subtilis and Bacilus amyloliquefacien, respectively. Fermentation with food-derived Isolate-2 and fish-derived F-9 increased crude protein content by 5.32% and 8.27%, respectively; improved the amino acid profile by increasing certain essential amino acids, broke down larger soy protein to 35 k Da and under, eliminated antigenicity against glycinin and β-conglycinin, and removed raffinose and stachyose in the soybean meal following a 24-h fermentation.Conclusions: Our results suggest these two B. amyloliquefaciens bacteria can efficiently solid state ferment soybean meal and ultimately produce a more utilizable food source for growing healthy piglets. | Samantha Medeiros Jingjing Xie Paul W.Dyce Hugh Y.Cai Kees De Lange Hongfu Zhang Julang Li | 2018 | Journal of Animal Science and Biotechnology2018，9，3： | 12 |
| 2 | Cross-cultural adaption and psychometric properties of the Chinese version of the Diabetes Behavior Rating Scale: a pilot study显示文摘Self-care behavior plays a major role in diabetic management. However, in China, a satisfactory instrument has not yet been developed to evaluate the compliance of self-care behavior for young patients with type 1 diabetes mellitus(T1 DM). The Diabetes Behavior Rating Scale(DBRS) has a potential to be the first mature instrument. The purpose of this study is to cross-culturally adapt the DBRS, and preliminarily evaluate its psychometric properties. The instrument translation included translation, back translation and culture adaptation. Psychometric properties were assessed in a sample of 116 young patients with T1 DM adapting insulin injection therapy. The Chinese version of the DBRS was divided to four subscales. Cronbach's α for the total scale was 0.92. The mean inter-item and item-total correlations were 0.35 and 0.54 respectively. Test-retest reliability showed good temporal stability(r=0.81, P=0.001). Negative correlations were found between DBRS scores with the Diabetes Distress Scale scores(r=.0.32, P=0.003) and hemoglobin A1 c(HbA1 c) levels(r=.0.36, P=0.002). Higher DBRS scores correlated with better glycemic control. The Chinese insulin injection therapy version of the DBRS is well translated and culturally adapted. It shows good overall reliability and validity and appears to be a valuable tool for assessing the diabetic self-care behaviors for young patients with T1 DM. | Jing Zhu Jingjing Xu Yang Chen Yong Gu Li Ji Yufeng Zhou Min Zhu Hsiang-Ting Hsu Xiaoping Huang Cuiping Yuan Yun Shi Dan Yan Lili Xie Shuang Chen Tao Yang Wei He | 2018 | Science China(Life Sciences)2018，61，3： | 6 |
| 3 | Microstructure optimization of core@shell structured MSe_(2)/FeSe_(2)@MoSe_(2)(M=Co,Ni)flower-like multicomponent nanocomposites towards high-efficiency microwave absorption显示文摘In this work,we put forward a scheme to exquisitely design and selectively synthesize the core@shell structured MSe_(2)/FeSe_(2)@MoSe_(2)(M=Co,Ni)flower-like multicomponent nanocomposites(MCNCs)through a simple two-step hydrothermal reaction on the surfaces of MFe_(2)O_4 nanospheres with the certain amounts of Mo and Se sources.With increasing the amounts of Mo and Se sources,the obtained core@shell structured MSe_(2)/FeSe_(2)@MoSe_(2)(M=Co,Ni)MCNCs with the enhanced content of MoSe_(2)and improved flower-like geometry morphology could be produced on a large scale.The obtained results revealed that the as-prepared samples displayed improved comprehensive microwave absorption properties(CMAPs)with the increased amounts of Mo and Se sources.The as-prepared CoSe_(2)/FeSe_(2)@MoSe_(2)and NiSe_(2)/FeSe_(2)@MoSe_(2)MCNCs with the well-defined flower-like morphology could simultaneously present the outstanding CMAPs in terms of strong absorption capability,wide absorption bandwidth,and thin matching thicknesses,which mainly originated from the conduction loss and flower-like geometry morphology.Therefore,the findings not only develop the very desirable candidates for high-performance microwave absorption materials but also pave a new way for optimizing the CMAPs through tailoring morphology engineering. | Jingjing Zhang Xiaosi Qi Xiu Gong Qiong Peng Yanli Chen Ren Xie Wei Zhong | 2022 | Journal of Materials Science & Technology2022，，33： | 5 |
| 4 | Abscisic acid and jasmonic acid are involved in drought priming-induced tolerance to drought in wheat显示文摘Drought stress is a limiting factor for wheat production and food security.Drought priming has been shown to increase drought tolerance in wheat.However,the underlying mechanisms are unknown.In the present study,the genes encoding the biosynthesis and metabolism of abscisic acid(ABA)and jasmonic acid(JA),as well as genes involved in the ABA and JA signaling pathways were up-regulated by drought priming.Endogenous concentrations of JA and ABA increased following drought priming.The interplay between JA and ABA in plant responses to drought priming was further investigated using inhibitors of ABA and JA biosynthesis.Application of fluridone(FLU)or nordihydroguaiaretic acid(NDGA)to primed plants resulted in lower chlorophyll-fluorescence parameters and activities of superoxide dismutase and glutathione reductase,and higher cell membrane damage,compared to primed plants(PD)under drought stress.NDGA+ABA,but not FLU+JA,restored priming-induced tolerance,as indicated by a finding of no significant difference from PD under drought stress.Under drought priming,NDGA induced the suppression of ABA accumulation,while FLU did not affect JA accumulation.These results were consistent with the expression of genes involved in the biosynthesis of ABA and JA.They suggest that ABA and JA are required for priming-induced drought tolerance in wheat,with JA acting upstream of ABA. | Xiao Wang Qing Li Jingjing Xie Mei Huang Jian Cai Qin Zhou Tingbo Dai Dong Jiang | 2021 | The Crop Journal2021，9，1： | 5 |
| 5 | NS1-binding protein radiosensitizes esophageal squamous cell carcinoma by transcriptionally suppressing c-Myc显示文摘Background:Cisplatin-based chemotherapy with concurrent radiotherapy is a standard treatment for advanced esophageal squamous cell carcinoma(ESCC).NS1-binding protein(NS1-BP),a member of the BTB-kelch protein fam-ily,has been shown to inhibit the proliferation of Hela cells by suppressing c-Myc.In the present study,we examined the potential function role of NS1-BP expression in ESCC,and particularly,the sensitivity of ESCC to radiotherapy.Methods:NS1-BP expression was examined using immunohistochemistry in two cohorts(n=98 for the training cohort;n=46 for independent validation cohort)of ESCC patients receiving cisplatin-based chemotherapy and con-current radiotherapy.Normal esophageal mucosal tissue blocks were used as a control.We also conducted a series of in vitro and in vivo experiments to examine the potential effects of over-expressing NS1-BP on ESCC cells,and particu-larly their sensitivity to ionizing irradiation.Results:In the training cohort,NS1-BP downregulation was observed in 59%(85/144)of the ESCC specimens.NS1-BP downregulation was associated with chemoradiotherapeutic resistance and shorter disease-specific survival(DSS)in both the training and validation cohorts.Over-expressing NS1-BP in cultured ESCC cells substantially increased the cellular response to irradiation both in vitro and in vivo.NS1-BP also significantly enhanced IR-induced apoptosis,and abrogated IR-induced G2/M cell-cycle arrest and ATM/Chk1 phosphorylation.Immunoprecipitation assays indicated that NS1-BP could interact with c-Myc promoter regions to inhibit its transcription.In ESCC tissues,c-Myc expression was inversely correlated with NS1-BP levels,and was associated with a shorter DSS.Conclusions:Our findings highlight the role and importance of NS1-BP in radiosensitivity of ESCC.Targeting the NS1-BP/c-Myc pathway may provide a novel therapeutic strategy for ESCC. | Yuwen Wang Jingjing Cheng Dan Xie Xiaofeng Ding Hailing Hou Xi Chen Puchun Er Furong Zhang Lujun Zhao Zhiyong Yuan Qingsong Pang Ping Wang Dong Qian | 2018 | Cancer Communications2018，38，1： | 4 |
| 6 | Genetic and Molecular Characterization of H9N2 Avian Influenza Viruses Isolated from Live Poultry Markets in Hubei Province, Central China, 2013–2017显示文摘H9N2 subtype avian influenza virus(AIV)is an influenza A virus that is widely spread throughout Asia,where it jeopardizes the poultry industry and provides genetic material for emerging human pathogens.To better understand the epidemicity and genetics of H9 subtype AIVs,we conducted active surveillance in live poultry markets(LPMs)in Hubei Province from 2013 to 2017.A total of 4798 samples were collected from apparent healthy poultry and environment.Realtime RT-PCR revealed that the positivity rate of influenza A was 26.6%(1275/4798),of which the H9 subtype accounted for 50.3%(641/1275)of the positive samples.Of the 132 H9N2 viral strains isolated,48 representative strains were subjected to evolutionary analysis and genotyping.Phylogenetic analysis revealed that all H9N2 viral genes had 91.1%–100%nucleotide homology,clustered with genotype 57,and had high homology with human H9N2 viruses isolated from2013 to 2017 in China.Using a nucleotide divergence cutoff of 95%,we identified ten distinct H9N2 genotypes that continued to change over time.Molecular analysis demonstrated that six H9N2 isolates had additional potential glycosylation sites at position 218 in the hemagglutinin protein,and all isolates had I155 T and Q226 L mutations.Moreover,44 strains had A558 V mutations in the PB2 protein and four had E627 V mutations,along with H9N2 human infection strains A/Beijing/1/2016 and A/Beijing/1/2017.These results emphasize that the H9N2 influenza virus in Hubei continues to mutate and undergo mammalian adaptation changes,indicating the necessity of strengthening the surveillance of the AIV H9N2 subtype in LPMs. | Zhibin Hu Fuhu Peng Zhenghui Xiong Wanpo Zhang Tingting Li Yuejun Shi Jun Xie Xin Jin Jingjing Huang Hongde Xiao Dingren Bi Nianhua Song Zili Li | 2021 | Virologica Sinica2021，36，2： | 4 |
| 7 | miR395-regulated sulfate metabolism exploits pathogen sensitivity to sulfate to boost immunity in rice显示文摘MicroRNAs (miRNAs) play important roles in plant physiological activities. However, their roles and molecular mechanisms in boosting plant immunity, especially through the modulation of macronutrient metabolism in response to pathogens, are largely unknown. Here, we report that an evolutionarily conserved miRNA, miR395, promotes resistance to Xanthomonas oryzae pv. oryzae (Xoo) and X. oryzae pv. oryzicola (Xoc), two destructive bacterial pathogens, by regulating sulfate accumulation and distribution in rice. Specifically, miR395 targets and suppresses the expression of the ATP sulfurylase gene OsAPS1, which functions in sulfate assimilation, and two sulfate transporter genes, OsSULTR2;1 and OsSULTR2;2, which function in sulfate translocation, to promote sulfate accumulation, resulting in broad-spectrum resistance to bacterial pathogens in miR395-overexpressing plants. Genetic analysis revealed that miR395-triggered resistance is involved in both pathogen-associated molecular pattern-triggered immunity and R gene-mediated resistance. Moreover, we found that accumulated sulfate but not S-metabolites inhibits proliferation of pathogenic bacteria, revealing a sulfate-mediated antibacterial defense mechanism that differs from sulfur-induced resistance. Furthermore, compared with other bacteria, Xoo and Xoc, which lack the sulfate transporter CysZ, are sensitive to high levels of extracellular sulfate. Accordingly, miR395-regulated sulfate accumulation impaired the virulence of Xoo and Xoc by decreasing extracellular polysaccharide production and biofilm formation. Taken together, these results suggest that rice miR395 modulates sulfate metabolism to exploit pathogen sensitivity to sulfate and thereby promotes broad-spectrum resistance. | Zeyu Yang Shugang Hui Yan Lv Miaojing Zhang Dan Chen Jingjing Tian Haitao Zhang Hongbo Liu Jianbo Cao Wenya Xie Changyin Wu Shiping Wang Meng Yuan | 2022 | Molecular Plant2022，15，4： | 3 |
| 8 | Heat stress-induced mucosal barrier dysfunction is potentially associated with gut microbiota dysbiosis in pigs显示文摘Heat stress(HS)can be detrimental to the gut health of swine.Many negative outcomes induced by HS are increasingly recognized as including modulation of intestinal microbiota.In turn,the intestinal microbiota is a unique ecosystem playing a critical role in mediating the host stress response.Therefore,we aimed to characterize gut microbiota of pigs’exposure to short-term HS,to explore a possible link between the intestinal microbiota and HS-related changes,including serum cytokines,oxidation status,and intestinal epithelial barrier function.Our findings showed that HS led to intestinal morphological and integrity changes(villus height,serum diamine oxidase[DAO],serum D-lactate and the relative expressions of tight junction proteins),reduction of serum cytokines(interleukin[IL]-8,IL-12,interferongamma[IFN-g]),and antioxidant activity(higher glutathione[GSH]and malondialdehyde[MDA]content,and lower superoxide dismutase[SOD]).Also,16S rRNA sequencing analysis revealed that although there was no difference in microbial a-diversity,some HS-associated composition differences were revealed in the ileum and cecum,which partly led to an imbalance in the production of short-chain fatty acids including propionate acid and valerate acid.Relevance networks revealed that HS-derived changes in bacterial genera and microbial metabolites,such as Chlamydia,Lactobacillus,Succinivibrio,Bifidobacterium,Lachnoclostridium,and propionic acid,were correlated with oxidative stress,intestinal barrier dysfunction,and inflammation in pigs.Collectively,our observations suggest that intestinal damage induced by HS is probably partly related to the gut microbiota dysbiosis,though the underlying mechanism remains to be fully elucidated. | Bing Xia Weida Wu Wei Fang Xiaobin Wen Jingjing Xie Hongfu Zhang | 2022 | Animal Nutrition2022，，1： | 3 |
| 9 | Changes in climate regimes over China based on a high-resolution dataset显示文摘During the 20th century the global climate has been shifted towards warmer and drier regimes, mainly due to anthropogenic warming [1,2]. Climate changes can notably disrupt the environment and ecosystems. To evaluate the impacts of climate change on ecoregions, the Kdppen-Trewartha (K-T) climate classification [3] is widely used because it was constructed on the basis of surface vegetation. The K-T classification system combines temperature, precipitation, and their seasonality into a single metric. Six major climate types and several sub-climate types are defined (A: tropical, B: dry, C: subtropical, D: temperate, E: subpolar, F: polar). Each K-T climate type is consistent with certain prevalent plant species. Definitions and criteria of K-T climate types were outlined by Feng et al.[4]. | Wei Huang Jingjing Yan Chang Liu Tingting Xie | 2019 | Science Bulletin2019，64，6： | 3 |
| 10 | Generation and characterization of stable pig pregastrulation epiblast stem cell lines显示文摘Pig epiblast-derived pluripotent stem cells are considered to have great potential and broad prospects for human therapeutic model developme nt and livestock breeding.Despite on going attempts since the 1990s,no stably defined pig epiblast-derived stem cell line has bee n established.Here,guided by in sights from a large-scale sin gle-cell tran scriptome an alysis of pig embryos from embryonic day(E)0 to E14,specifically,the tracing of pluripotency changes during epiblast development,we developed an in vitro culture medium for establishing and maintaining stable pluripotent stem cell lines from pig E10 pregastrulation epiblasts(pgEpiSCs).Enabled by chemical inhibition of WNT-related signaling in combination with growth factors in the FGF/ERK,JAK/STAT3,and Activin/Nodal pathways,pgEpiSCs maintain their pluripotency transcriptome features,similar to those of E10 epiblast cells,and normal karyotypes after more than 240 passages and have the potential to differentiate into three germ layers.Strikingly,ultradeep in situ Hi-C analysis revealed functional impacts of chromatin 3D-spatial associations on the transcriptional regulation of pluripote ncy marker genes in pgEpiSCs.I n practice,we con firmed that pgEpiSCs readily tolerate at least three rounds of successive gene editi ng and gene rated cloned gen e-edited live piglets.Our findings deliver on the long-a nticipated promise of pig pluripote nt stem cells and open new avenues for biological research,animal husbandry,and regenerative biomedicine. | Minglei Zhi Jinying Zhang Qianzi Tang Dawei Yu Shuai Gao Dengfeng Gao Pengliang Liu Jianxiong Guo Tang Hai Jie Gao Suying Cao Zimo Zhao Chongyang Li Xiaogang Weng Mengnan He Tianzhi Chen Yingjie Wang Keren Long Deling Jiao Guanglei Li Jiaman Zhang Yan Liu Yu Lin Daxin Pang Qianqian Zhu Naixin Chen Jingjing Huang Xinze Chen Yixuan Yao Jingcang Yang Zicong Xie Xianya Huang Mengxin Liu Ran Zhang Qiuyan Li Yiliang Miao Jianhui Tian Xingxu Huang Hongsheng Ouyang Bofeng Liu Wei Xie Qi Zhou Zhonghua Liu Caihong Zheng Mingzhou Li Jianyong Han | 2022 | Cell Research2022，32，4： | 3 |
| 11 | Geochemistry of arsenic and selenium in a Ge-poor coal from the Wulantuga coal-hosted Ge ore deposit,Inner Mongolia,North China显示文摘This paper reports new data for arsenic(As)and selenium(Se)in a total of twelve bench samples of Ge-rich and Ge-poor coals in the No.6 coal seam from the Wulantuga ore deposit,Inner Mongolia,Northeastern China.The Gepoor coals are characterized by low-ash(with a weighted average ash yield 10.59%).The coal samples were digested using an UltraClave microwave high pressure reactor(milestone)and trace elements were detected by inductively coupled plasma mass spectrometry—collision/reaction cell technology,a reliable method for As and Se determination in coal samples.The contents of As and Se in the Ge-poor(with a weighted average content of 9.14 and 0.30 lg/g,respectively)and Ge-rich coal samples in the present study(varies from 16.88 to 17,776 lg/g and from 0.26 to 14.39 lg/g,respectively)are in a sharp contrast.The As in the Ge-poor coals is of both organic-and pyrite-associations,and its enrichment is attributed to the sediment source,and to a lesser extent,to hydrothermal fluids.Se is predominantly connected with organic matter in the Wulantuga Ge-poor coals. | Panpan Xie Qingqian Li Jingjing Liu Hongjian Song Jianpeng Wei | 2014 | International Journal of Coal Science & Technology2014，1，4： | 3 |
| 12 | Geochemistry of rare earth elements and yttrium in a Ge-poor coal from the Wulantuga ore deposit, Inner Mongolia, North China显示文摘This paper reports data of yttrium and rare earth(REY,or REE if Y is considered)in a Ge-poor(the average value of Ge is 0.57 μg/g)coal from the Wulantuga ore deposit,middle Inner Mongolia,northern China.The contents of yttrium and rare earth in six coal samples of the Wulantuga ore deposit were detected by inductively coupled plasma mass spectrometry.The content of yttrium and rare earth in the study area varies from 5.88 to 119.19 μg/g,with an average of 61.85 μg/g,similar to the average value for world coals.The light-REY and heavy-REY enrichments are the two major REY-enrichment types of the Ge-poor coal from the Wulantuga ore deposit,and the top and bottom parts of the coal seam have a higher content of REY than the middle part.The sources of yttrium and rare earth in the Ge-poor coal of Wulantuga may be mainly associated with terrigenous materials and natural waters enriched in yttrium and rare earth. | Jingjing Liu Yaofa Jiang Panpan Xie Qingqian Li | 2014 | International Journal of Coal Science & Technology2014，1，4： | 3 |
| 13 | Related-Key Impossible Differential Cryptanalysis of LBlock显示文摘A related-key impossible differential attack on 24-round LBlock is constructed by using new 16-round related-key impossible differentials and adding 4rounds at the top and 4 rounds at the bottom of these 16-round related-key impossible differential paths. The data and time complexities are about 2^(63) chosen plaintexts and2^(75.42)24-round encryptions respectively. | XIE Min LI Jingjing ZANG Yuechuan | 2017 | Chinese Journal of Electronics2017，26，1： | 3 |
| 14 | Fasudil alleviates LPS-induced lung injury by restoring aquaporin 5 expression and inhibiting inflammation in lungs显示文摘Fasudil, a selective rho kinase(ROCK) inhibitor, has been reported to play a beneficial role in systemic inflammation in acute lung injury, but its mechanism for ameliorating pulmonary edema and inflammation remains unclear. Using hematoxylin-and-eosin(H&E) staining, immunohistochemistry, enzyme-linked immunosorbent assay,quantitative real time PCR and Western blotting, we found that fasudil attenuated LPS-induced lung injury, decreased lung edema, and suppressed inflammatory responses including leukocyte infiltration and IL-6 production. Further,fasudil upregulated LPS-induced aquaporin 5 reduction and inhibited NF-κB activation in the lungs of mice. Our results suggest that fasudil could restore the expression of aquaporin 5 to eliminate LPS-induced lung edema and prevent LPS-induced pulmonary inflammation by blocking the inflammatory pathway. Collectively, blockade of the ROCK pathway by fasudil may be a potential strategy for the treatment of acute lung injury. | Jingjing Wang Hui Kong Jian Xu Yanli Wang Hong Wang Weiping Xie | 2019 | The Journal of Biomedical Research2019，33，3： | 3 |
| 15 | EEG processing and its application in brain-computer interface显示文摘Electroencephalogram (EEG) is an efficient tool in exploring human brains. It plays a very important role in diagnosis of disorders related to epilepsy and development of new interaction techniques between machines and human beings,namely,brain-computer interface (BCI). The purpose of this review is to illustrate the recent researches in EEG processing and EEG-based BCI. First,we outline several methods in removing artifacts from EEGs,and classical algorithms for fatigue detection are discussed. Then,two BCI paradigms including motor imagery and steady-state motion visual evoked potentials (SSMVEP) produced by oscillating Newton's rings are introduced. Finally,BCI systems including wheelchair controlling and electronic car navigation are elaborated. As a new technique to control equipments,BCI has promising potential in rehabilitation of disorders in central nervous system,such as stroke and spinal cord injury,treatment of attention deficit hyperactivity disorder (ADHD) in children and development of novel games such as brain-controlled auto racings. | Wang Jing Xu Guanghua Xie Jun Zhang Feng Li Lili Han Chengcheng Li Yeping Sun Jingjing | 2013 | Engineering Sciences2013，11，1： | 3 |
| 16 | Inhibition of Japanese Encephalitis Virus Infection by Flavivirus Recombinant E Protein Domain Ⅲ显示文摘Japanese encephalitis virus (JEV) is a mosquito-borne flavivirus closely related to the human pathogens including yellow fever virus, dengue virus and West Nile virus. There are currently no effective antiviral therapies for all of the flavivirus and only a few highly effective vaccines are licensed for human use. In this paper, the E protein domain III (DIII) of six heterologous flaviviruses (DENV1-4, WNV and JEV) was expressed in Escherichia coli successfully. The proteins were purified after a solubilization and refolding procedure, characterized by SDS-PAGE and Western blotting. Competitive inhibition showed that all recombinant flavivirus DIII proteins blocked the entry of JEV into BHK-21 cells. Further studies indicated that antibodies induced by the soluble recombinant flavivirus DIII partially protected mice against lethal JEV challenge. These results demonstrated that recombinant flavivirus DIII proteins could inhibit JEV infection competitively, and immunization with proper folding flavivirus DIII induced cross-protection against JEV infection in mice, implying a possible role of DIII for the cross-protection among flavivirus as well as its use in antigens for immunization in animal models. | Jingjing Fan Yi Liu Xuping Xie Bo Zhang Zhiming Yuan | 2013 | Virologica Sinica2013，28，3： | 3 |
| 17 | NS1结合蛋白通过转录抑制c-Myc增强食管鳞状细胞癌的放疗敏感性显示文摘背景与目的基于顺铂的化疗联合放疗是晚期食管鳞状细胞癌(esophageal squamous cell carcinoma,ESCC)的标准疗法。NS1结合蛋白(NS1-binding protein,NS1-BP)是BTB-kelch蛋白家族中的一员,已被证明可通过抑制c-Myc进而抑制HeLa细胞增殖。在本研究中,我们检测NS1-BP表达在ESCC中的潜在作用,特别是其在ESCC放疗敏感性方面的作用。方法对于两组接受基于顺铂化疗且联合放疗的ESCC患者(研究组n=98;验证组n=46),采用免疫组化方法分别检测NS1-BP表达。使用正常食管黏膜组织块作为对照。我们也进行了一系列体外和体内实验来检测过表达NS1-BP对ESCC细胞各方面的潜在影响,尤其关注的是对电离辐射的敏感性方面的影响。结果在研究组中,59%(85/144)的ESCC样本观察到了NS1-BP下调。在研究组和验证组中,NS1-BP下调与放化疗抵抗和较短的疾病特异生存期(disease-specific survival,DSS)均具有相关性。体内和体外培养条件下过表达NS1-BP均能大幅度提高ECSS细胞对放疗的敏感性。NS1-BP也显著增强IR诱导的凋亡,阻断IR诱导的G2/M细胞周期阻滞和ATM/Chk1磷酸化。免疫沉淀反应显示NS1-BP可以与c-Myc启动子区相互作用从而抑制其转录。在ESCC组织中,c-Myc表达与NS1-BP水平负相关,与较短的疾病特异生存期相关。结论我们的研究结果显示了NS1-BP在ESCC放疗敏感性中的作用和重要性。以NS1-BP/c-Myc通路为靶标有望开发一种新的ESCC治疗策略。 | Yuwen Wang Jingjing Cheng Dan Xie Xiaofeng Ding Hailing Hou Xi Chen Puchun Er Furong Zhang Lujun Zhao Zhiyong Yuan Qingsong Pang Ping Wang Dong Qian | 2019 | 癌症2019，38，2： | 2 |
| 18 | Construction of a shared system-based real-world clinical research system显示文摘贡献主要疾病实体的确定的研究门诊病人诊所研究的繁体中文药的湖北省的医院由基础、到国家繁体中文药治疗和临床的研究信息的构造分享的国家繁体中文药临床的研究进行了系统。可以看开发真实世界的繁体中文药临床的研究范例,这些诊所探索了从临床的研究,健康管理,和特征诊断和治疗的方面构造研究门诊病人诊所的模式。 | Huikun Wu Xiaodong Li Fan Yang Dan Xie Hui Li Jingjing Huang Mingxing Guo | 2014 | Frontiers of Medicine2014，8，3： | 2 |
| 19 | Histopathological and imageological studies on clinical outcomes of mineralized collagen reconstruction rod for femoral head necrosis with one case report显示文摘In this article,the biodegradation process and bone formation of a mineralized collagen reconstruction rod embedding in necrosis of human femoral head were investigated by imageological and histological methods.Computed radiography(CR)computerized tomography(CT),common pathological section and hard tissue section analysis were used to evaluated the dynamics of imageological and histopathological changes of femoral head,interface between the host bone and implant and the bone reconstruction process.The results showed that the density of rods increased closed to that of host bones after 1 year implanting,and the interface between them turns to blurring.Hard tissue grinding sections analysis showed osteocytes appearing in sparse bone trabecular and bone pit region,as well as a few vessels in the degraded dye powder matrix were noticed,indicating the new bone forming between the implants and host bones.Regular decalcified sections analysis showed scattered osteoclasts,multinucleated giant cells and fibrosis components existing in the degraded rod and the host bone trabecular.Degraded debris was endocytosed by giant cells,and vascular network formed around the boundaries of the implanted rod.The good osteointegration has been expressed by the interface between the implanted rod and the host bone becoming blurred.Histological results indicated that the implanted rod degradation process and new bones regeneration simultaneously occurred around the boundaries of embedding rod.New bone and host bone were hinged and co-existed. | Baogang Xie Hao Wang Jianhua Hao Ping Wang Na Zhang Jingjing Wu Zhiye Qiu Fuzhai Cui | 2017 | Regenerative Biomaterials2017，4，4： | 2 |
| 20 | Alisol B 23-acetate-induced HepG2 hepatoma cell death through mTOR signaling-initiated G_1 cell cycle arrest and apoptosis: A quantitative proteomic study显示文摘Objective: The present study aimed to investigate the molecular events in alisol B 23-acetate(ABA) cytotoxic activity against a liver cancer cell line.Methods: First, we employed a quantitative proteomics approach based on stable isotope labeling by amino acids in cell culture(SILAC) to identify the different proteins expressed in HepG2 liver cancer cells upon exposure to ABA. Next, bioinformatics analyses through DAVID and STRING on-line tools were used to predict the pathways involved. Finally, we applied functional validation including cell cycle analysis and Western blotting for apoptosis and mTOR pathway-related proteins to confirm the bioinformatics predictions.Results: We identified 330 different proteins with the SILAC-based quantitative proteomics approach. The bioinformatics analysis and the functional validation revealed that the mTOR pathway, ribosome biogenesis, cell cycle, and apoptosis pathways were differentially regulated by ABA. G1 cell cycle arrest, apoptosis and mTOR inhibition were confirmed.Conclusions: ABA, a potential mTOR inhibitor, induces the disruption of ribosomal biogenesis. It also affects the mTOR-MRP axis to cause G1 cell cycle arrest and finally leads to cancer cell apoptosis. | Ji Xia Qiang Luo Shengbin Huang Fuquan Jiang Lin Wang Guanghui Wang Jingjing Xie Jie Liu Yang Xu | 2019 | Chinese Journal of Cancer Research2019，31，2： | 2 |
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