4篇
您的检索式：作者名="XINLAN ZHOU"
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| 1 | A stringent dual control system overseeing transcription and activity of the Cre recombinase for the liver-specific conditional gene knock-out mouse model显示文摘Liver cancer is one of the most threatening diseases in Chinese population. Just like in other tissues, tumor initiation and development in liver involve multiple steps of genetic and epigenetic alterations with several unknown details. However, unlike in other tissues, a tis-sue specific inducible Cre recombinase system that allows temporal and spatial deletion of a target DNA fragment is still not available for in vivo functional gene annotation in hepatocytes. In our pursuit to establish such a mouse model, we designed a dual inducible Cre transgene system and tested it in cultured cells. By combining a CCAAT/enhancer binding protein β (C/EBP β) promoter derived Tet-off expression system and the estrogen receptor (ER) mediated functional control, we show a desirable profile of both hepatocyte-specificity and regulability of the Cre expression in a series of critical assessments in the cell culture system, which provides confidence in continua-tion of our ongoing pursuit in mouse. | Yu Wu Yinghua He Hongyu Zhang Xinlan Dai Xiaoyu Zhou Jun Gu Guan Wang Jingde Zhu | 2008 | Journal of Genetics and Genomics2008，35，7： | 3 |
| 2 | Isolation of mesophyll protoplasts from tea(Camellia sinensis)and localization analysis of enzymes involved in the biosynthesis of specialized metabolites显示文摘Specialized metabolites in tea(Camellia sinensis)are fundamental quality factors.It is important to characterize gene function in vivo to identify key enzymes and reactions involved in the biosynthesis of such metabolites.Here we report a transient expression method to analyze gene function in isolated tea mesophyll protoplasts.This method is an alternative approach to traditional genetic transformation for studies on gene function in vivo.We screened several tea cultivars and different digestion conditions to optimize protoplast isolation.Digestion of newly emerged leaves of C.sinensis‘Zhongbai 4’with 3%cellulase R-10 and 0.3%macerozyme R-10 for about 12 h yielded approximately 107 mesophyll protoplasts.Genes encoding enzymes involved in secondary metabolite synthesis were transiently expressed in the protoplasts,and their subcellular locations were determined.With further improvements in the transfection efficiency,this transient expression system will contribute greatly to the analyses of in vivo gene function in tea. | Ying Zhou Rufang Deng Xinlan Xu Ziyin Yang | 2021 | Beverage Plant Research2021，1，1： | 1 |
| 3 | Experimental Realization of a quantum algorighm显示文摘 | Vandersypen L M K Xinlan Zhou | 1998 | Nature1998，393，： | 1 |
| 4 | A pan-cancer analysis identifies SOAT1 as an immunological and prognostic biomarker显示文摘Sterol o-acyltransferase1(SOAT1)is an enzyme that regulates lipid metabolism.Nevertheless,the predictive value of SOAT1 regarding immune responses in cancer is not fully understood.Herein,we aimed to expound the predictive value and the potential biological functions of SOAT1 in pan-cancer.Raw data related to SOAT1 expression in 33 different types of cancer were acquired from The Cancer Genome Atlas(TCGA)and Genotype-Tissue Expression(GTEx)databases.SOAT1 expression was significantly increased in most cancers and showed a distinct correlation with prognosis.This enhanced expression of the SOAT1 gene was confirmed by evaluating SOAT1 protein expression using tissue microarrays.In addition,we found significant positive associations between SOAT1 expression levels and infiltrating immune cells,including T cells,neutrophils,and macrophages.Moreover,the co-expression analysis between SOAT1 and immune genes showed that many immune-related genes were increased with enhanced SOAT1 expression.A gene set enrichment analysis(GSEA)revealed that the expression of SOAT1 correlated with the tumor microenvironment,adaptive immune response,interferon signaling,and cytokine signaling.These findings indicate that SOAT1 is a potential candidate marker for predicting prognosis and a promising target for tumor immunotherapy in cancers. | YANGQING HUANG XINLAN ZHOU XIUFEN LI DAN HUANG ZHONG FANG RONGRONG DING | 2023 | Oncology Research2023，31，2： | 0 |
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