|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | 3D printed fiber-optic nanomechanical bioprobe显示文摘Ultrasensitive nanomechanical instruments,e.g.atomic force microscopy(AFM),can be used to perform delicate biomechanical measurements and reveal the complex mechanical environment of biological processes.However,these instruments are limited because of their size and complex feedback system.In this study,we demonstrate a miniature fiber optical nanomechanical probe(FONP)that can be used to detect the mechanical properties of single cells and in vivo tissue measurements.A FONP that can operate in air and in liquids was developed by programming a microcantilever probe on the end face of a single-mode fiber using femtosecond laser two-photon polymerization nanolithography.To realize stiffness matching of the FONP and sample,a strategy of customizing the microcantilever’s spring constant according to the sample was proposed based on structure-correlated mechanics.As a proof-of concept,three FONPs with spring constants varying from 0.421 N m^(−1)to 52.6 N m^(−1)by more than two orders of magnitude were prepared.The highest microforce sensitivity was 54.5 nmμN^(−1)and the detection limit was 2.1 nN.The Young’s modulus of heterogeneous soft materials,such as polydimethylsiloxane,muscle tissue of living mice,onion cells,and MCF-7 cells,were successfully measured,which validating the broad applicability of this method.Our strategy provides a universal protocol for directly programming fiber-optic AFMs.Moreover,this method has no special requirements for the size and shape of living biological samples,which is infeasible when using commercial AFMs.FONP has made substantial progress in realizing basic biological discoveries,which may create new biomedical applications that cannot be realized by current AFMs. | Mengqiang Zou Changrui Liao Yanping Chen Lei Xu Shuo Tang Gaixia Xu Ke Ma Jiangtao Zhou Zhihao Cai Bozhe Li Cong Zhao Zhourui Xu Yuanyuan Shen Shen Liu Ying Wang Zongsong Gan Hao Wang Xuming Zhang Sandor Kasas Yiping Wang | 2023 | International Journal of Extreme Manufacturing2023，5，1： | 1 |
| 2 | Cell-Based Biosensors Based on Light-Addressable Potentiometric Sensors for Single Cell Monitoring显示文摘 | Xu Gaixia Ye Xuesong Qing Lifeng Xu Ying Li Yah Li Rong Wang Ping | 2005 | Biosensors and Bioelectronics2005，20，： | 1 |
| 3 | Improvement in Resolution of Multiphoton Scanning Structured Illumination Microscopy via Harmonics显示文摘We describe a multiphoton(mP)-structured illumination microscopy(SIM)technique,which demonstrates substantial improvement in image resolution compared with linear SIM due to the nonlinear response of fluorescence.This nonlinear response is caused by the effect of nonsinusoidal structured illumination created by scanning a sinusoidally modulated illumination to excite an mP fluorescence signal.The harmonics of the structured fluorescence illumination are utilised to improve resolution.We present an mP-SIM theory for reconstructing the super-resolution image of the system.Theoretically,the resolution of our m P-SIM is unlimited if all the high-order harmonics of the nonlinear response of fluorescence are considered.Experimentally,we demonstrate an 86 nm lateral resolution for two-photon(2P)-SIM and a 72 nm lateral resolution for second-harmonic-generation(SHG)-SIM.We further demonstrate their application by imaging cells stained with F-actin and collagen fibres in mouse-tail tendon.Our method can be directly used in commercial mP microscopes and requires no specific fluorophores or high-intensity laser. | Lei Wang Xiaomin Zheng Jie Zhou Meiting Wang Jiajie Chen Youjun Zeng Gaixia Xu Ying Wang Haixia Qiu Yonghong Shao Junle Qu Bruce Zhi Gao Ying Gu | 2022 | Engineering2022，，9： | 1 |
| 4 | Cell-based biosensors and its application in biomedicine显示文摘 | Wang Ping Xu Gaixia Qin Lifeng | 2005 | Sensors Actuators B2005，108，： | 1 |
| 5 | A novel multi-functional cell-based microphysiometer显示文摘这篇论文基于 MLAPS 在生活房间为几细胞外的离子集中的同时的大小和行动潜力测量论述新奇多功能的 microphysiometer (多光设法解决的 potentiometric 传感器) 。在 microphysiometer,一些敏感的膜与光在工作频率,和每个频率部件的反应振幅采购的 n 同时被照亮能被测量由并行处理联机算法。在实验,在西方的药的效果下面的细胞外的环境 H +, Na +, K +, Ca2 + 的关系(苯,苯巴比妥钠) 并且中国药(黄芩,欧楂树,铁杉欧芹) 被分析,并且几药的效果被评估。而且,不同房间类型(心脏的 myocytes 和神经原) 的行动潜力信号能被臀部测量并且分析。由检测这些参数,系统能监视房间新陈代谢和行动潜力的即时进程,观察膜界限受体的不同类型的功能的回答,并且评估药的活动。 | XU Ying XU Gaixia LIU Qingjun CAI Hua LI Yan LI Rong WANG Ping | 2006 | Progress in Natural Science:Materials International2006，16，8： | 1 |
| 6 | Cell-based biosensors:Towards the development of cellular monitoring显示文摘Cell-based biosensors (CBBs), a research hot-spot of biosensors, which treat living cells as sensing elements, can detect the functional information of biologically active analytes. They characterize with high sensitivity, excellent selectivity and rapid response, and have been applied in many fields, such as biomedicine, environmental monitoring and pharmaceutical screening. Recently cell-cultured technology, silicon microfabrication technology and genetic technology have promoted exploration of CBBs dramatically. To elucidate the novel research findings and applications of cell- based biosensors, this paper summarizes various research approaches, presents some challenges and proposes the research trends. | Gaixia Xu Yicong Wu Rong Li Ping Wang Weiming Yan Xiaoxiang Zheng | 2002 | Chinese Science Bulletin2002，47，22： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员