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| 1 | OsCERK1-Mediated Chitin Perception and Immune Signaling Requires Receptor-like Cytoplasmic Kinase 185 to Activate an MAPK Cascade in Rice显示文摘保存联系病原体的分子的模式(PAMP ) 例如几丁质,被在激活 mitogen 的蛋白质 kinase (MAPK ) 的快速的激活串联的宿主细胞表面和扳机定位的模式识别受体(PRR ) 察觉,它为植物抵抗被要求到病原体。然而,到在植物的 MAPK 激活的从 PAMP 感觉的直接连接仍然保持大部分未知。在这研究,我们发现联系 PRR 的像受体的细胞质的 kinase Oryza sativa RLCK185 播送到通过和 MAPK kinase kinase 的相互作用表明串联的 MAPK 的从 PAMP 受体 OsCERK1 的有免疫力的发信号, OsMAPKKK,它是 MAPK 串联的起始的 kinase。OsRLCK185 交往与并且 phosphorylates OsMAPKKK 的 C 终端规章的领域。phosphomimetic OsRLCK185 和 OsMAPKKK 的 Coexpression 在 Nicotiana benthamiana 叶子激活 MAPK3/6 phosphorylation。而且, OsMAPKKK 交往与并且 phosphorylates OsMKK4,关键 MAPK kinase 那 transduces 几丁质信号。OsMAPKKK 的 Overexpression 增加导致几丁质的 MAPK3/6 激活,而 OsMAPKKK 击倒的妥协导致几丁质的 MAPK3/6 激活和抵抗到米饭强风真菌。一起拿,我们的结果在米饭从房间表面几丁质感觉建议一条发信号 phospho 小径的存在到 MAPK 串联的细胞内部的激活。 | Chao Wang Gang Wang Chi Zhang Pinkuan Zhu Huiling Dai Nan Yu Zuhua He Ling Xu Ertao Wang | 2017 | Molecular Plant2017，10，4： | 27 |
| 2 | miR-126 enhances the sensitivity of non-small cell lung cancer cells to anticancer agents by targeting vascular endothelial growth factor A显示文摘增加的证据建议 hsa-miR-126 (miR-126 ) 在房间衬里的非小的房间肺癌症(NSCLC ) 是下面调整的, miR-126 的恢复由指向特定的分子损害肿瘤房间增长,移植,侵略,和幸存。这里,我们第一次报导 miR-126 涉及调整 NSCLC 房间的反应到癌症化疗。在有 miR-126 的房间模仿的 transfected A549 或禁止者以后,我们发现 miR-126 的提高的水平显著地与减少的一半被联系 adriamycin ( ADM )和长春新碱的最大的禁止的集中, ADM 的增加的累积,脉管的 endothelial 生长因素 A ( VEGFA )和 multidrug 的下面规定联系抵抗的蛋白质 1 ( MRP1 ),并且 Akt 的 inactivation 发信号的小径。而且,提高了 miR-126 的表示压制了 A549 异种皮移植的生长并且禁止了 VEGFA 和 MRP1 的表示。miR-126 能高效地通过和 VEGFA 3-untranslated 区域的相互作用的下面调整 VEGFA 表示,而 VEGFA 的恢复能部分由 miR-126 稀释 MRP1 的抑制。然而, LY294002,表明小径的 PI3K/Akt 的一个禁止者,减少这效果,建议 miR-126 的那提高的表情通过发信号的 VEGF/PI3K/Akt/MRP1 的否定规定增加了 NSCLC 房间的敏感到 anticancer 代理人小径。 | Xiaolan Zhu Hao Li Lulu Long Lulu Hui Haining Chen Xuefeng Wang Huiling Shen Wenlin Xu | 2012 | Acta Biochimica et Biophysica Sinica2012，44，6： | 23 |
| 3 | Wood defect detection method with PCA feature fusion and compressed sensing显示文摘We used principal component analysis(PCA) and compressed sensing to detect wood defects from wood plate images. PCA makes it possible to reduce data redundancy and feature dimensions and compressed sensing, used as a classifier, improves identification accuracy. We extracted 25 features, including geometry and regional features, gray-scale texture features, and invariant moment features, from wood board images and then integrated them using PCA, and selected eight principal components to express defects. After the fusion process, we used the features to construct a data dictionary, and realized the classification of defects by computing the optimal solution of the data dictionary in l_1 norm using the least square method. We tested 50 Xylosma samples of live knots, dead knots, and cracks. The average detection time with PCA feature fusion and without were 0.2015 and 0.7125 ms,respectively. The original detection accuracy by SOM neural network was 87 %, but after compressed sensing, it was 92 %. | Yizhuo Zhang Chao Xu Chao Li Huiling Yu Jun Cao | 2015 | Journal of Forestry Research2015，26，3： | 17 |
| 4 | Construction and Functional Analysis of a Lentiviral Expression Vector Containing a Scavenger Receptor (SR-PSOX) that Binds Uniquely Phosphatidylserine and Oxidized Lipoprotein显示文摘这研究的目的是构造包含特别地绑与的 scavenger 受体(SR-PSOX ) 的 lentiviral 表达式向量有六个 histidine 标签并且到的 phosphatidylserine 和氧化脂蛋白在动脉粥样硬化调查 SR-PSOX 的函数。我们利用有效在 vitro 或 invivo 交付目标基因进用提高的 biosafetyreplication 无能的 lentivirus 划分和非划分的哺乳动物的房间的 ViraPower lentiviral 表达式系统。钝结束的顺序用克隆反应的 reversetranscription 聚合酶链反应和方向性的 TOPO 被放大。通过一双 thecytomegalovirus 提交教材和 SR-PSOX 的反向的教材,正确克隆被聚合酶链反应识别并且定序。包装混合 andSR-PSOX-pLenti6/V5 TOPO 表示原生质标志的 ViraPower 是进 293FT 房间线 usingLipofectamine 的 co-transfected 2000。内长、外长的 SR-PSOX 以及在在不同时间的各种各样的泡沫房间模型的 - α蛋白质削尖的肿瘤坏死因素(TNF ) 的表示被 sodiumdodecyl sulfate-polyacrylamide 凝胶电泳,蛋白质印迹和间接 immunofluorescenceassay 检测。蛋白质印迹和免疫荧光分析证实α蛋白质是起来的 SR-PSOX andTNF- 的表情在泡沫房间模型调整了。我们的数据建议过去表示 ofrecom-binant 人 SR-PSOX 蛋白质能支持泡沫房间形成并且起来调整煽动性的因素 TNF- α的表示。 | Zhihua QUAN Huiling YANG Yongzong YANG Bin YAN Renxian CAO Gebo WEN Changhui LIU Yangyan XU | 2007 | Acta Biochimica et Biophysica Sinica2007，39，3： | 16 |
| 5 | A Highly Pathogenic Strain of Porcine Deltacoronavirus Caused Watery Diarrhea in Newborn Piglets显示文摘Porcine deltacoronavirus(PDCoV) is a newly identified virus that causes watery diarrhea in newborn piglets and results in significant economic losses to the pig industry. Since first reported in Hong Kong in 2012, PDCoV has been subsequently detected in USA, South Korea, Thailand, and China's Mainland. Here we isolated a strain of PDCoV, named CHN-GD-2016,from the intestinal content of a diseased newborn piglet with severe diarrhea in a pig farm in Guangdong, China. PDCoV CHN-GD-2016 could be identified by immunofluorescence with PDCoV specific rabbit antisera, and typical crown-shaped particles with spiky surface projections of this PDCoV were observed with electron microscopy. Genomic analysis showed that the PDCoV CHN-GD-2016 was closely related to other Chinese PDCoV strains, with the highest sequence similarity with the strain CHN/Tianjin/2016. Importantly, inoculation of newborn piglets with 1×10~5 TCID_(50) of CHN-GD-2016 by oral feeding successfully reproduced clear clinical symptoms, including vomiting, dehydration, and severe diarrhea in piglets. In addition, the virus RNA in rectal swabs from 1 to 7 days post inoculation was detected, macroscopic and microscopic lesions in small intestine were observed, and viral antigen was also detected in the small intestines with immunohistochemical staining. Collectively, the data show in this study confirms that PDCoV is present in Guangdong,China and is highly pathogenic in newborn piglets. | Zhichao Xu Huiling Zhong Qingfeng Zhou Yunping Du Li Chen Yun Zhang Chunyi Xue Yongchang Cao | 2018 | Virologica Sinica2018，33，2： | 16 |
| 6 | Flexible fiber-shaped supercapacitors based on hierarchically nanostructured composite electrodes显示文摘新奇所有固态的、同轴的、塑造纤维的不对称的 supercapacitor 被在 MnO 上包一张进行的复写纸制作了 < 潜水艇 class= “ a-plus-plus ” > 2 -modified nanoporous 金牌电线。这根精力电线展出 12 mF | Henghui Xu Xianluo Hu Yongming Sun Huiling Yang Xiaoxiao Liu Yunhui Huang | 2015 | Nano Research2015，8，4： | 14 |
| 7 | Clinical characteristics and effectiveness of antiviral agents in hospitalized children with infectious mononucleosis in China:A multicenter retrospective study显示文摘Importance:The clinical characteristics of infectious mononucleosis(IM)in Chinese children have not been evaluated in multicenter studies,and the effectiveness of antiviral treatment are controversial.Objective:To investigate the clinical characteristics of Chinese children with IM and current status of antiviral therapy for affected patients.Methods:Hospitalized patients with IM were enrolled between 2018 and 2020 in five children’s hospitals in China.The clinical characteristics were compared among four age groups:<3 years,3-<6 years,6-<10 years,and≥10 years.The clinical characteristics of IM and effectiveness of antiviral therapy were compared among patients receiving acyclovir(ACV),ganciclovir(GCV),and no antiviral therapy(i.e.,non-antiviral group).Results:In total,499 patients were analyzed;most patients were 3-<6 years of age.The most common symptoms and signs included fever(100%),lymphadenopathy(98.6%),pharyngitis(86.4%),eyelid edema(76.8%),and snoring(72.9%).There were significant differences in rash,hepatomegaly,and liver dysfunction among the four age groups.Patients aged<3 years had a lower incidence of liver dysfunction and a higher incidence of rash.Among the 499 patients,50.1%were treated with GCV,26.3%were treated with ACV,and 23.6%received no antiviral therapy.Compared with the non-antiviral group,patients in the ACV and GCV groups had longer durations of fever(P<0.001).There were no significant differences in the incidences of complications among the three treatment groups.Interpretation:The incidence of IM in Chinese children peaked at 3-<6 years of age.Clinical features of IM varied according to age.Patients receiving antiviral therapy exhibited more serious clinical manifestations than did patients without antiviral therapy.The effectiveness of antiviral therapy for IM requires further analysis. | Huili Hu Huiling Deng Jing Bi Yi Xu Shuangjie Li Yue Xie Xinrong Sun Dongmeng Wang Xufang Li Wenxian Ouyang Bing Hu Yufeng Zhang He Tang Chunxiao Fang Hui Zhang Lingyun Guo Chen Wang Tianyi Wang Fengxia Yang Tao Jiang Zhengde Xie Gang Liu | 2021 | Pediatric Investigation2021，5，3： | 12 |
| 8 | Photoreduction of CO_2 to methanol over Bi_2S_3/CdS photocatalyst under visible light irradiation显示文摘The Bi2S3,CdS and Bi2S3/CdS photocatalysts were prepared by direct reactions between their corresponding salt and thiourea in a hy- drothermal autoclave.The photocatalytic activities of these photocatalysts for reducing CO2 to CH3OH under visible light irradiation have been investigated.The results show that the photocatalytic activity and visible light response of Bi2S3 are higher than those of CdS.The Bi2S3 modification can enhance the photocatalytic activity and visible light response of CdS.The photocatalytic activity of Bi2S3/CdS hetero-junction photocatalyst was the highest and the highest yields of methanol was 613μmol/g when the weight proportion of Bi2S3 to CdS was 15%,which was about three times as large as that of CdS or two times of that of Bi2S3. | Xin Li Juntao Chen Huiling Li Jingtian Li Yitao Xu Yingju Liu Jiarong Zhou | 2011 | Journal of Natural Gas Chemistry2011，20，4： | 11 |
| 9 | Effectiveness and safety of moxibustion in treatment of lumbar disc herniation: a systematic review and Meta-analysis显示文摘OBJECTIVE: To evaluate the effectiveness and safety of moxibustion therapy in the treatment of lumbar disc herniation (LDH). METHODS: Four Chinese databases and three English databases were searched from their inception to April 2018.Randomized controlled trials (RCTs) were included if moxibustion was used as the sole treatment or as a part of combination therapy with other treatments in patients with LDH. Two reviewers independently extracted the data and assessed the methodological quality using the Cochrane criteria for the risk of bias. The Meta-analysis was performed using Review Manager 5.3 software. RESULTS: In total, 16 RCTs including 1186 patients with LDH were analyzed. The Meta-analysis showed favorable effects of moxibustion in combination with massage therapy on the visual analog scale score compared with massage therapy alone [mean difference (MD)=? 1.32, 95% confidence interval (CI)(? 2.12,? 0.51), P = 0.001]. The subgroup Meta-analysis failed to show favorable effects of electro-acupuncture plus moxibustion on the efficacy rate compared with electro-acupuncture alone [relative risk (RR)= 1.06, 95% CI (0.98, 1.14), P = 0.15]. However, acupuncture or massage therapy plus moxibustion improved the efficacy rates compared with acupuncture or massage therapy alone [RR = 1.33, 95% CI (1.18, 1.49), P < 0.000 01][χ^2 = 2.76, P = 0.25, I2 = 27%],[RR = 1.15, 95% CI (1.06, 1.25), P = 0.001][χ^2 = 0.00, P = 0.95, I2 = 0%]. With respect to the Japanese Orthopaedic Association (JOA) scores, acupuncture or massage therapy in addition to moxibustion produced results different from those of acupuncture or massage therapy alone [MD = 5.58, 95% CI (4.15, 7.00), P < 0.000 01][χ^2 = 0.58, P = 0.45, I2 = 0%],[MD = 3.61, 95% CI (3.01, 4.21), P < 0.000 01]. There were no significant differences in the JOA score for subjective symptoms, objective symptoms, daily living ability, and other parameters. In six RCTs, no adverse reactions occurred during moxibustion. CONCLUSION: Whether moxibustion is an effective intervention for LDH is unclear because of the small sample size of qualified RCTs and the high risk of bias. More high-quality RCTs that overcome the methodological shortcomings of the existing evidence are needed. | Wang Yang Zhang Huiling Xia Liping Sun Zhiling Xu Xiao Du Shizheng | 2019 | Journal of Traditional Chinese Medicine2019，39，5： | 11 |
| 10 | Berberine sensitizes ovarian cancer cells to cisplatin through miR-21/PDCD4 axis显示文摘最近的研究证明了 microRNA-21 (miR-21 ) 贡献肿瘤抵抗到化疗。有趣地,我们发现了 berberine 能在几根癌症房间线禁止 miR-21 表示。在这研究,我们调查了 berberine 是否能调制卵巢的癌症房间的敏感到 cisplatin 并且探索机制。被孵化, berberine 与 cisplatin 结合了的 cisplatin 抵抗的 SKOV3 房间独自比 cisplatin 有 signicantly 更低的幸存组和提高的导致 cisplatin 的 apoptosis。Berberine 能在卵巢的癌症禁止 miR-21 表示和功能,由它的目标 PDCD4 的改进出现在卵巢的癌症的重要肿瘤 suppressor。结果建议 berberine 能经由在卵巢的癌症房间调整 miR-21/PDCD4 轴调制 cisplatin 的敏感。 | Shiguo Liu Yue Fang Huiling Shen Wenlin Xu Hao Li | 2013 | Acta Biochimica et Biophysica Sinica2013，45，9： | 9 |
| 11 | Photocatalytic reduction of carbon dioxide to methanol by Cu_2O/SiC nanocrystallite under visible light irradiation显示文摘The Cu2O/SiC photocatalyst was obtained from SiC nanoparticles (NPs) modified by Cu2O. Their photocatalytic activities for reducing CO2 to CH3OH under visible light irradiation have been investigated. The results indicated that besides a small quantity of 6H-SiC, SiC NPs mainly consisted of 3C-SiC. The band gaps of SiC and Cu2O were estimated to be about 1.95 and 2.23 eV from UV-Vis spectra, respectively. The Cu2O modification can enhance the photocatalytic performance of SiC NPs, and the largest yields of methanol on SiC, Cu2O and Cu2O/SiC photocatalysts under visible light irradiation were 153, 104 and 191μmol/g, respectively. | Huiling Li Yonggen Lei Ying Huang Yueping Fang Yuehua Xu Li Zhu Xin Li | 2011 | Journal of Natural Gas Chemistry2011，20，2： | 8 |
| 12 | Focal adhesion protein Kindlin-2 regulates bone homeostasis in mice显示文摘Our recent studies demonstrate that the focal adhesion protein Kindlin-2 is critical for chondrogenesis and early skeletal development. Here, we show that deleting Kindlin-2 from osteoblasts using the 2.3-kb mouse Col1 a1-Cre transgene minimally impacts bone mass in mice, but deleting Kindlin-2 using the 10-kb mouse Dmp1-Cre transgene, which targets osteocytes and mature osteoblasts, results in striking osteopenia in mice. Kindlin-2 loss reduces the osteoblastic population but increases the osteoclastic and adipocytic populations in the bone microenvironment. Kindlin-2 loss upregulates sclerostin in osteocytes,downregulates β-catenin in osteoblasts, and inhibits osteoblast formation and differentiation in vitro and in vivo. Upregulation ofβ-catenin in the mutant cells reverses the osteopenia induced by Kindlin-2 deficiency. Kindlin-2 loss additionally increases the expression of RANKL in osteocytes and increases osteoclast formation and bone resorption. Kindlin-2 deletion in osteocytes promotes osteoclast formation in osteocyte/bone marrow monocyte cocultures, which is significantly blocked by an anti-RANKLneutralizing antibody. Finally, Kindlin-2 loss increases osteocyte apoptosis and impairs osteocyte spreading and dendrite formation.Thus, we demonstrate an important role of Kindlin-2 in the regulation of bone homeostasis and provide a potential target for the treatment of metabolic bone diseases. | Huiling Cao Qinnan Yan Dong Wang Yumei Lai Bo Zhou Qi Zhang Wenfei Jin Simin Lin Yiming Lei Liting Ma Yuxi Guo Yishu Wang Yilin Wang Xiaochun Bai Chuanju Liu Jian QFeng Chuanyue Wu Di Chen Xu Cao Guozhi Xiao | 2020 | Bone Research2020，8，1： | 8 |
| 13 | Small Interfering RNA-mediated Caveolin-1 Knockout on Plasminogen Activator Inhibitor-1 Expression in Insulin-stimulated Human Vascular Endothelial Cells显示文摘把人的脉管的 endothelial 房间(ECV304 ) 用作目标,我们学习了 caveolin (CAV ) 的效果在 plasminogen 的刺激胰岛素的表示期间的 -1 禁止者(白族)-1。代表 RNAi CAV-1 基因的适当搁浅单人赛的 oligonucleotides 被 Ambion 软件分析。在退火产生双 stranded oligonucleotides (dsoligo ) 以后,它被克隆进由 T4 DNA 连接酶包含 RNA 聚合酶 III 表示元素的 pENTR/U6 入口向量。从 pENTR/U6 入口转移的短发卡(shRNA ) 序列与 LR 再结合反应被克隆进 pLenti6/BLOCK-iT-DEST 向量。由定序的 Afteridentification,我们成功地用网关技术构造了 CAV-1 RNAi lentiviral 表达式系统。Silencing 效率是由即时 reversetranscription 聚合酶链反应,免疫荧光染色并且西方的弄污的 assayed。ECV304cells 在包含胰岛素的不同集中的媒介是有教养的(1x10 ^( 有 CAV-1 基因 silenced 的 -9)to1x10^(-7) M ) 。表示水平和潜水艇 PAI-1 和 CAV-1 的细胞的本地化用反向的抄写聚合酶链反应,免疫荧光染色和蛋白质印迹试金被比较。结果证明有势力抑制 ofCAV-1 表示能到达 85% ,并且它对 CAV-1-derived shRNA,不是导出 theS100A13 的 shRNA 特定。在蛋白质确实在房间膜下面积累了的在与生理的胰岛素,而是 PAI-1 孵化的 RNAi^+andRNAi^- ECV304 房间之间的 PAI-1 表示没有戏剧的差别。当胰岛素的集中增加了, PAI-1 的表示是起来调整的,而 CAV-1 的表示稀释了。而且, PAI-1 清楚地在 CAV-1knockdown 以后扩充了。这些结果显示 hyperinsulinism 能由 inhibitingCAV-1 支持 PAI-1 表示,并且稳定或在 endothelial 房间的起来调整的 CAV-1 表示可能在糖尿病减少大容器和毛状的容器的复杂并发症。 | Huiling YANG Shuya HE Zhihua QUAN Weixia PENG Bin YAN Jianghua LIU Fang WEN Renxian CAO Yangyan XU Gebo WEN Weixin HU | 2007 | Acta Biochimica et Biophysica Sinica2007，39，3： | 7 |
| 14 | miR-137 restoration sensitizes multidrug-resistant MCF-7/ADM cells to anticancer agents by targeting YB-1显示文摘到化学疗法的代理人的 Multidrug 抵抗(MDR ) 是到在乳癌病人的成功的治疗的一个主要障碍。这研究的目的是调查 miR-137 是否涉及 MDR 的规定,并且在 MCF-7/ADM 房间的敏感上探索 miR-137 的机制。miR-137 是在 MCF-7/ADM 房间的 downregulated,并且它的表达式被发现相反地在乳癌房间与 Y 盒子有约束力的 protein-1 (YB-1 ) 和 P-glycoprotein (P-gp ) 层次相关。而且, YB-1 被酶记者试金和西方的污点分析作为 miR-137 的一个目标证实。而且, miR-137 的提高的表示减少了 YB-1 和 P-gp 的蛋白质表示层次, mimicking 在到 anticancer 代理人的 MCF-7/ADM 房间的敏感击倒的 YB-1 的效果,而 YB-1 的恢复减少了这效果。在结论,我们的结果证明 miR-137 被指向 YB-1 通过 P-gp 的调整在癌症涉及 MDR,建议 miR-137 可能是为阻止并且逆行在肿瘤房间的 MDR 的一个潜在的目标。 | Xiaolan Zhu Yuefeng Li Huiling Shen Hao Li Lulu Hui Wenlin Xu | 2013 | Acta Biochimica et Biophysica Sinica2013，45，2： | 6 |
| 15 | miR-106a confers cisplatin resistance by regulating PTEN/Akt pathway in gastric cancer cells显示文摘最近的研究证明了 microRNA-106a (miR-106a ) 是在胃的癌症的 overexpressed 并且贡献肿瘤生长。在这研究,我们调查了 miR-106a 是否调停了到化学疗法的代理人 cisplatin (DDP ) 的胃的癌症房间线 SGC7901 的抵抗。MiR-106a 表示与它的父母线 SGC7901 相比在 DDP 抵抗房间线 SGC7901/DDP 是起来调整的。miR-106a 的 Transfection 在 SGC7901 导致了 DDP 抵抗,当在 SGC7901/DDP 的 miR-106a 的抑制导致了提高的 DDP cytotoxicity 时。进一步的学习显示 miR-106a-induced DDP 抵抗的机制包含了从染色体 10 删除的磷酸酶和 tensin 相当或相同的事物(PTEN ) 的表示蛋白质和它的下游的 phosphatidylinositol 3 kinase (PI3K )/protein kinase B (AKT ) 小径。这研究在胃的癌症提供 DDP 抵抗的新奇机制。 | Yue Fang Huiling Shen Hao Li Yuan Cao Rong Qin Lulu Long Xiaolan Zhu Chanjuan Xie Wenlin XU | 2013 | Acta Biochimica et Biophysica Sinica2013，45，11： | 6 |
| 16 | Development of a multiplex one-step real-time RT-PCR assay for the simultaneous detection of eight viruses associated with febrile rash illnesses显示文摘Fever and rash illnesses(FRIs)are a series of common diseaseswith fever and rashes as clinicalmanifestations,most of which are caused by viral infection.The rashes of FRIs are generally nonspecific;therefore it is difficult to identify FRIassociated viruses solely based on clinical symptoms.To achieve rapid and accurate identification of FRI pathogens,a multiplex one-step real-time reverse transcription-polymerase chain reaction(RT-PCR)assay was developed and evaluated in this study.Primers and probes were selected for the detection of measles virus(MeV),rubella virus(RV),human enterovirus(EV),varicella-zoster virus(VZV),dengue virus(DENV),human parvovirus B19(B19),Epstein-Barr virus(EBV),and human herpes virus 6(HHV-6),which cover the most common pathogenic viruses of FRIs.Detection of the eight FRI-associated viruses,which was divided into two groups/tubes,was simultaneously performed under universal optimized reaction conditions in multiplex one-step real-time RT-PCR assay.The multiplex realtime RT-PCR showed high sensitivity and specificity in detecting the eight FRI-associated viruses.The limits of detection(LODs)for the eight viruses were in the range of 47–177 copies/reaction,and no cross reactions for the eight FRIassociated viruses were found in the multiplex assay.In addition,the results of the multiplex real-time RT-PCR assay were consistent with the results of a monoplex real-time RT-PCR assay and sequencing for clinical specimens obtained from FRI patients.With its advantages of high efficiency and rapid and accurate diagnosis,multiplex real-time RT-PCR was very feasible for the early diagnosis of FRI pathogenic viruses and would be of great help for the proper treatment,monitoring,and initiation of preventive measures for FRI cases. | Aili Cui Shulei Wang Qiang Zhang Huiling Wang Zhen Zhu Aqian Li Qinqin Song Yanzhe Hao Jilan He Wenbo Xu Yan Zhang | 2020 | Biosafety and Health2020，2，2： | 5 |
| 17 | Constructing Straight Pores and Improving Mechanical Properties of GangueBased Porous Ceramics显示文摘The large-scale accumulation and pollution of solid mining waste is an urgent issue.Coal gangue is a prominent type of solid waste,and shows promise for use in high value-added products due to its content of many important compounds,including SiO_(2) and Al2O3.This study proposed the preparation of highly porous ceramics from coal gangue,coal slime,and coconut palm fibers.The ceramics were produced at a sintering temperature of 950℃ with a fiber content of 6 wt%,which led to the formation of porous ceramics with a porosity of 66.93%,volume density of 1.0329 g/cm^(3),compressive strength of 1.1025 MPa,and thermal conductivity is 0.3919 K(W/mk).A finite element model of the porous ceramics was established using the Abaqus module in ANSYS software,where the stress distribution and compressive strength were simulated.Further,the relationship between porosity and compressive strength was analyzed.The thermal properties of the porous ceramics were analyzed using the Fluent module,where the simulated changes in porosity under various sintering temperatures were consistent with the experimental data.The preparation of this highly porous ceramic from solid waste coal gangue shows promise for the minimizing the impact of waste gas and wastewater pollution in the future. | Hang Xu Huiling Du Le Kang Qiudi Cheng Danni Feng Siyu Xia | 2021 | Journal of Renewable Materials2021，9，12： | 4 |
| 18 | MOF-Derived ZnS Nanodots/Ti_(3)C_(2)T_(x) MXene Hybrids Boosting Superior Lithium Storage Performance显示文摘ZnS has great potentials as an anode for lithium storage because of its high theoretical capacity and resource abundance;however,the large volume expansion accompanied with structural collapse and low conductivity of ZnS cause severe capacity fading and inferior rate capability during lithium storage. Herein,0D-2 D ZnS nanodots/Ti_(3)C_(2)T_x MXene hybrids are prepared by anchoring ZnS nanodots on Ti_(3)C_(2)T_(x) MXene nanosheets through coordination modulation between MXene and MOF precursor(ZIF-8) followed with sulfidation. The MXene substratecoupled with the ZnS nanodots can synergistically accommodate volume variation of ZnS over charge–discharge to realize stable cyclability. As revealed by XPS characterizations and DFT calculations,the strong interfacial interaction between ZnS nanodots and MXene nanosheets can boost fast electron/lithium-ion transfer to achieve excellent electrochemical activity and kinetics for lithium storage. Thereby,the as-prepared ZnS nanodots/MXene hybrid exhibits a high capacity of 726.8 mAh g^(-1) at 30 mA g^(-1),superior cyclic stability(462.8 mAh g^(-1) after 1000 cycles at 0.5 A g^(-1)),and excellent rate performance. The present results provide new insights into the understanding of the lithium storage mechanism of ZnS and the revealing of the e ects of interfacial interaction on lithium storage performance enhancement. | Bin Cao Huan Liu Xin Zhang Peng Zhang Qizhen Zhu Huiling Du Lianli Wang Rupeng Zhang Bin Xu | 2021 | Nano-Micro Letters2021，13，12： | 3 |
| 19 | PI3K/AKT Mediated p53 Down-Regulation Participates in CpG DNA Inhibition of Spontaneous B Cell Apoptosis显示文摘unmethylated CpG DNA 能阻止 B 房间的自发的 apoptosis。然而, CpG DNA 由堵住 apoptosis 的精确机制仍然保持不清楚。在这研究,我们证明 B 房间 apoptosis 被 CpG DNA 的增加显著地禁止。CpG DNA 的处理能减少 caspase 的表示 3,增加国际机场和 Bcl-xL 表情,并且禁止水平在 B 房间被增加的 p53 蛋白质表示在 24 h 的自发的 apoptosis。AKT kinase 活动与 CpG DNA 的孵化被增加。wortmannin 和 Ly294002 能由 CpG DNA 从 apoptosis 废除 B 房间的保护。当由特定的禁止者 Ly294002 堵住 PI3K 时,由 CpG DNA 的 Bci-xL 和国际机场的起来规定没被禁止,当由 CpG DNA 的 p53 的抑制能被 Ly294002 堵住时。这些结果证明由 CpG DNA 的自发的 B 房间 apoptosis 的抑制被相关到 p53 和 caspase 的 Bci-xL,国际机场和下面规定的起来规定 3。p53 的 CpG DNA 抑制通过 PI3K/AKT 发信号被调停。细胞与分子的免疫学。2009; 6 (3 ): 175-180。 | Yongxin Zhou Huiling Zhen Yunqing Mei Yongwu Wang Jing Feng Shuchang Xu Xiaoying Fu | 2009 | Cellular & Molecular Immunology2009，6，3： | 3 |
| 20 | Targeted nanomedicines remodeling immunosuppressive tumor microenvironment for enhanced cancer immunotherapy显示文摘Cancer immunotherapy has significantly flourished and revolutionized the limited conventional tumor therapies,on account of its good safety and long-term memory ability.Discouragingly,low patient response rates and potential immune-related side effects make it rather challenging to literally bring immunotherapy from bench to bedside.However,it has become evident that,although the immunosuppressive tumor microenvironment(TME)plays a pivotal role in facilitating tumor progression and metastasis,it also provides various potential targets for remodeling the immunosuppressive TME,which can consequently bolster the effectiveness of antitumor response and tumor suppression.Additionally,the particular characteristics of TME,in turn,can be exploited as avenues for designing diverse precise targeting nanomedicines.In general,it is of urgent necessity to deliver nanomedicines for remodeling the immunosuppressive TME,thus improving the therapeutic outcomes and clinical translation prospects of immunotherapy.Herein,we will illustrate several formation mechanisms of immunosuppressive TME.More importantly,a variety of strategies concerning remodeling immunosuppressive TME and strengthening patients'immune systems,will be reviewed.Ultimately,we will discuss the existing obstacles and future perspectives in the development of antitumor immunotherapy.Hopefully,the thriving bloom of immunotherapy will bring vibrancy to further exploration of comprehensive cancer treatment. | Yanyan Xu Jingyuan Xiong Xiyang Sun Huile Gao | 2022 | Acta Pharmaceutica Sinica B2022，12，12： | 3 |
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