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| 1 | The Epitope Study on the SARS-CoV Nucleocapsid Protein显示文摘The nucleocapsid protein (N protein) has been found to be an antigenic protein in a number of coronaviruses. Whether the N protein in severe acute respiratory syndrome-associated coronavirus (SARS-CoV) is antigenic remains to be elucidated. Using Western blot and Enzyme-linked Immunosorbent Assay (ELISA),the recombinant N proteins and the synthesized peptides derived from the N protein were screened in sera from SARS patients. All patient sera in this study displayed strong positive immunoreactivities against the recombinant N proteins,whereas normal sera gave negative immunoresponses to these proteins, indicating that the N protein of SARS-CoV is an antigenic protein. Furthermore, the epitope sites in the N protein were determined by competition experiments, in which the recombinant proteins or the synthesized peptides competed against the SARS-CoV proteins to bind to the antibodies raised in SARS sera. One epitope site located at the C-terminus was confirmed as the most antigenic region in this protein. A detailed screening of peptide with ELISA demonstrated that the amino sequence from Codons 371 to 407 was the epitope site at the C-terminus of the N protein. Understanding of the epitope sites could be very significant for developing an effective diagnostic approach to SARS. | Shuting Li, Liang Lin, Hao Wang, Jianning Yin, Yan Ren, Zhe Zhao, Jie Wen, Cuiqi Zhou, Xumin Zhang, Xiaolei Li, Jingqiang Wang, Zhengfeng Zhou, Jinxiu Liu, Jianmin Shao, Tingting Lei, Jianqiu Fang, Ningzhi Xu, and Siqi LiuBeijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China & Beijing Proteomics Institute, Beijing 101300, China | 2003 | Genomics, Proteomics & Bioinformatics2003，1，3： | 9 |
| 2 | Spatial energy efficiency patterns and the coupling relationship with industrial structure: A study on Liaoning Province, China显示文摘用在辽宁省的 14 个府级的城市的一件样品,这研究首先从下列三个观点探索了空间层次和精力效率的结构的特征:精力技术效率基于数据包封分析,精力消费每 GDP,和精力利用效率联合起来联合以前的二个索引。在在每个城市里测量并且分析工业结构的前进,合理性,和集中以后,我们在辽宁关于精力效率和工业结构的联合特征做了一些归纳,用联合的度等级模型。一些我们的结论如下:(1 ) 14 个城市在他们的精力效率显著地不同,与享受最高的精力效率的沈阳, Dalian,鞍山,和 Jinzhou。象 Fushun 和 Benxi 那样的西北的辽宁和另外的重工业的城市属于低效率、高消费的区域。(2 ) 在有更高的效率的区域,精力的空间模式技术效率,分别地,精力消费每 GDP,和精力利用效率联合起来是,塑造的 II 和 H- 。地理上,精力利用效率从东方显示出不同趋势到西方并且从北方到南方。象经济开发的 binuclear 结构那样的因素在精力效率的这个空间模式上有主要效果。(3 ) 东南的辽宁享受高度先进的工业结构。有高度合理的工业结构的区域形成 H 形状,与在二杆的沈阳和 Dalian。在中间、南部的辽宁的城市的凝块有高度集中的工业结构。(4 ) 总的来说,在精力效率和工业结构之间联合在辽宁是低的,除了在两结束的沈阳和 Dalian,在在先进工业结构和精力效率之间联合比在另外的城市里高的地方。 | GUAN Wei XU Shuting | 2015 | Journal of Geographical Sciences2015，25，3： | 8 |
| 3 | Highly active zigzag-like Pt-Zn alloy nanowires with high-index facets for alcohol electrooxidation显示文摘The development of highly efficient Pt-based alloy nanocatalysts is important but remains challenging for fuel cells commercialization.Here,a new class of zigzag-like platinum-zinc (Pt-Zn) alloy nanowires (NWs) with rough surface and controllable composition is reported.The merits of anisotropic one-dimensional nanostructure,stable high-index facets and coordinatively unsaturated Pt sites endow the composition-optimal Pt94Zn6 NWs with a mass activity of 7.2 and 6.2 times higher than that of commercial Pt black catalysts toward methanol/ethanol oxidation,respectively.Alloying-induced d-band electron modulation and lattice strain effects weaken the adsorption strength of poisoning species,which originally enhances the catalytic activity of Pt-Zn NWs.This study provides a new perspective of Pt-Zn electrocatalysts with intrinsic mechanism for enhanced catalytic performance. | Yanchao Xu Xiaoqiang Cui Shuting Wei Qinghua Zhang Lin Gu Fanqi Meng Jinchang Fan Weitao Zheng | 2019 | Nano Research2019，12，5： | 7 |
| 4 | Study on the promotion of lymphocytes in patients with COVID-19 by broad-spectrum chemokine receptor inhibitor vMIP-Ⅱ and its Mechanism of signal transmission in vitro显示文摘Dear Editor,COVID-19 cases showed a significant decrease in the number of peripheral CD4^(+) and CD8^(+)T cells accompanied by overactivation of cells.1 HHV8-derived viral macrophage inflammatory protein vMIP-Ⅱ is the only broad-spectrum chemokine receptor inhibitor that binds to three subtribes of chemokine receptors,which can significantly increase the specific cell immune response in infected organisms and have an effect of reducing plasma viral load on the viremia period.24 Here we report the effect of vMIP-Ⅱ in COVID-19. | Shiyu Li Shuting Liu Zhenyou Jiang Lixia Feng Yong Gao Youyu Chen Anding Xu Wenhua Huang Nuofu Zhang Hanxiao Sun | 2021 | Signal Transduction and Targeted Therapy2021，6，4： | 5 |
| 5 | Microcavity top-emission perovskite light-emitting diodes显示文摘Light-emitting diodes(LEDs)based on perovskites show great potential in lighting and display applications.However,although perovskite films with high photoluminescence quantum efficiencies are commonly achieved,the efficiencies of perovskite LEDs are largely limited by the low light out-coupling efficiency.Here,we show that high-efficiency perovskite LEDs with a high external quantum efficiency of 20.2% and an ultrahigh radiant exitance up to 114.9mWcm^(−2) can be achieved by employing the microcavity effect to enhance light extraction.The enhanced microcavity effect and light outcoupling efficiency are confirmed by the study of angle-dependent emission profiles.Our results show that both the optical and electrical properties of the device need to be optimized to achieve high-performance perovskite LEDs. | Yanfeng Miao Lu Cheng Wei Zou Lianghui Gu Ju Zhang Qiang Guo Qiming Peng Mengmeng Xu Yarong He Shuting Zhang Yu Cao Renzhi Li Nana Wang Wei Huang Jianpu Wang | 2020 | Light(Science & Applications)2020，9，1： | 4 |
| 6 | The formation of perovskite multiple quantum well structures for high performance light-emitting diodes显示文摘Recent works showed that high efficient perovskite light-emitting diodes can be achieved from solution-processed,self-organized multiple quantum wells(MQWs)with an energy cascade.We investigate how the mixing of QWs with different band gaps can affect the perovskite LED device performance.We find that the annealing process can significantly affect the constitution of the MQWs films,where the dominant phase can evolve from large band gap QWs to small band gap QWs.The optimal constitution for LED application lies in a transition point of small-n QWs dominant phase to large-n QWs dominant phase,when the MQW film presents highest photoluminescence while still remains uniform film morphology. | Yan Sun Li Zhang Nana Wang Shuting Zhang Yu Cao Yanfeng Miao Mengmeng Xu Hao Zhang Hai Li Chang Yi Jianpu Wang Wei Huang | 2018 | npj Flexible Electronics2018，2，1： | 4 |
| 7 | Prediction of nonlinear pilot-induced oscillation using an intelligent human pilot model显示文摘This paper proposes a method to predict nonlinear Pilot-Induced Oscillation(PIO)using an intelligent human pilot model.This method is based on a scalogram-based PIO metric,which uses wavelet transforms to analyze the nonlinear characteristics of a time-varying system.The intelligent human pilot model includes three modules:perception module,decision and adaptive module,and execution module.Intelligent and adaptive features,including a neural network receptor,fuzzy decision and adaptation,are also introduced into the human pilot model to describe the behavior of the human pilot accommodating the nonlinear events.Furthermore,an algorithm is proposed to describe the procedure of the PIO prediction method with nonlinear evaluation cases.The prediction results obtained by numerical simulation are compared with the assessments of flight test data to validate the utility of the method.The flight test data were generated in the evaluation of the Smart-Cue/Smart-Gain,which is capable of reducing the PIO tendencies considerably.The results show that the method can be applied to predict the nonlinear PIO events by human pilot model simulation. | Shuting XU Wenqian TAN Xiangju QU Cheng ZHANG | 2019 | Chinese Journal of Aeronautics2019，32，12： | 3 |
| 8 | Physicochemical properties and structure of fluid at nano-/micro-interface:Progress in simulation and experimental study显示文摘In modern chemical engineering processes, the involvement of solid/fluid interface is the most important component of process intensification techniques, such as confined membrane separation and catalysis. In the review, we summarized the research progress of the latest theoretical and experimental works to elucidate the contribution of interface to the fluid properties and structures at nano-and micro-scale. We mainly focused on water, alcohol aqueous solution, and ionic liquids, because they are classical systems in interfacial science and/or widely involved in the industrialization process. Surface-induced fluids were observed in all reviewed systems and played a critical role in physicochemical properties and structures of outside fluid. It can even be regarded as a new interface, when the adsorption layer has a strong interaction with the solid surface. Finally, we proposed a perspective on scientific challenges in the modern chemical engineering processes and outlined future prospects. | Qingwei Gao Yumeng Zhang Shuting Xu Aatto Laaksonen Yudan Zhu Xiaoyan Ji Xiaohua Lu | 2020 | Green Energy & Environment2020，5，3： | 3 |
| 9 | Level set-based isogeometric topology optimization for maximizing fundamental eigenfrequency显示文摘Maximizing the fundamental eigenfrequency is an efficient means for vibrating structures to avoid resonance and noises.In this study,we develop an isogeometric analysis(IGA)-based level set model for the fonnulation and solution of topology optimization in cases with maximum eigenfrequency.The proposed method is based on a combination of level set method and IGA technique,which uses the non-uniform rational B-spline(NURBS),description of geometry,to perfonn analysis.The same NURBS is used for geometry representation,but also for IGA-based dynamic analysis and parameterization of the level set surface,that is,the level set function.The method is applied to topology optimization problems of maximizing the fundamental eigenfrequency for a given amount of material.A modal track method,that monitors a single target eigenmode is employed to prevent the exchange of eigenmode order number in eigenfrequency optimization.The validity and efficiency of the proposed method are illustrated by benchmark examples. | Manman XU Shuting WANG Xianda XIE | 2019 | Frontiers of Mechanical Engineering2019，14，2： | 3 |
| 10 | Isogeometric topology optimization based on energy penalization for symmetric structure显示文摘We present an energy penalization method for isogeometric topology optimization using moving morphable components(ITO–MMC),propose an ITO–MMC with an additional bilateral or periodic symmetric constraint for symmetric structures,and then extend the proposed energy penalization method to an ITO–MMC with a symmetric constraint.The energy penalization method can solve the problems of numerical instability and convergence for the ITO–MMC and the ITO–MMC subjected to the structural symmetric constraint with asymmetric loads.Topology optimization problems of asymmetric,bilateral symmetric,and periodic symmetric structures are discussed to validate the effectiveness of the proposed energy penalization approach.Compared with the conventional ITO–MMC,the energy penalization method for the ITO–MMC can improve the convergence rate from 18.6%to 44.5%for the optimization of the asymmetric structure.For the ITO–MMC under a bilateral symmetric constraint,the proposed method can reduce the objective value by 5.6%and obtain a final optimized topology that has a clear boundary with decreased iterations.For the ITO–MMC under a periodic symmetric constraint,the proposed energy penalization method can dramatically reduce the number of iterations and obtain a speedup of more than 2. | Xianda XIE Shuting WANG Ming YE Zhaohui XIA Wei ZHAO Ning JIANG Manman XU | 2020 | Frontiers of Mechanical Engineering2020，15，1： | 3 |
| 11 | Small interfering RNA delivery to the neurons near the amyloid plaques for improved treatment of Alzheimer’s disease显示文摘Gene therapy represents a promising treatment for the Alzheimer’s disease(AD). However,gene delivery specific to brain lesions through systemic administration remains big challenge. In our previous work, we have developed an siRNA nanocomplex able to be specifically delivered to the amyloid plaques through surface modification with both CGN peptide for the blood–brain barrier(BBB)penetration and QSH peptide for β-amyloid binding. But, whether the as-designed nanocomplex could indeed improve the gene accumulation in the impaired neuron cells and ameliorate AD-associated symptoms remains further study. Herein, we prepared the nanocomplexes with an siRNA against β-site amyloid precursor protein-cleaving enzyme 1(BACE1), the rate-limiting enzyme of Aβ production, as the therapeutic siRNA of AD. The nanocomplexes exhibited high distribution in the Aβ deposits-enriched hippocampus, especially in the neurons near the amyloid plaques after intravenous administration. In APP/PS1 transgenic mice, the nanocomplexes down-regulated BACE1 in both mRNA and protein levels,as well as Aβ and amyloid plaques to the level of wild-type mice. Moreover, the nanocomplexes significantly increased the level of synaptophysin and rescued memory loss of the AD transgenic mice without hematological or histological toxicity. Taken together, this work presented direct evidences that the design of precise gene delivery to the AD lesions markedly improves the therapeutic outcome. | Qian Guo Xiaoyao Zheng Peng Yang Xiaoying Pang Kang Qian Pengzhen Wang Shuting Xu Dongyu Sheng Liuchang Wang Jinxu Cao Wei Lu Qizhi Zhang Xinguo Jiang | 2019 | Acta Pharmaceutica Sinica B2019，9，3： | 2 |
| 12 | 空间模式的学习和在中国的精力 eco 效率的空间效果显示文摘精力 eco 效率是集成从精力利用产生的生态、经济的好处的一个概念并且在 energy-environment-economy 系统用作效率的一项措施。就不受欢迎的输出而言用基于呆滞的措施(SBM ) 当模特儿,这研究首先从 1997 ~ 2012 在中国测量省的精力 eco 效率。它然后从三个方面分析精力 eco 效率的空间分发和进化:空间模式的规模,紧张,和谷物。最后,它借助于一个空间 econometric 模型在不同的省检验空间溢出效果和精力 eco 效率的影响因素。下列结论被得出:(1 ) 全面精力 eco 效率在中国是相对低的,与为大约 40% 的精力低效的区域财务。当 Ningxia, Gansu, Qinghai,和 Xinjiang 是有低效率的代表性的区域时,广东,海南和福建省享受最高的精力 eco 效率。因此,瓷器全面精力 eco 效率的进化的模式是 U 字形的。在本地区域之中,进化的四个主要模式被发现:增加,波动,变异,并且铺平。(2 ) 在省的水平,瓷器精力 eco 效率全球性并且局部地展示重要空间凝块。高高度的凝块主要在东方、南部的沿海的区域和低低的凝块发生在西北的区域和黄河的中间的活动范围。在空间模式的变化与高度低、低高度的凝块主要在区域发生了,与发生在 Beijing-Tianjin-Hebei 区域的最显著的变化。(3 ) 在那里存在在在中国的省之中的精力 eco 效率的重要空间效果。为一个给定的区域的精力 eco 效率,从邻近的区域的空间溢出在邻近的区域超过错误的影响。工业结构在精力 eco 效率上有最大的影响。 | GUAN Wei XU Shuting | 2016 | Journal of Geographical Sciences2016，26，9： | 2 |
| 13 | Recent Progresses in Lanthanide Metal-Organic Frameworks(Ln-MOFs) as Chemical Sensors for Ions, Antibiotics and Amino Acids显示文摘Various ions and antibiotics,widely used in industry and clinical medicine,respectively,are massively discharged to atmosphere and water,resulting in severe pollutions on environment and potential threats to human health.Besides,amino acids,the primary substances for the establishment of proteins,cells and tissues,are crucial to human health.Therefore,seeking effective and practicable materials to detect aforesaid analytes is vitally meaningful.Metal-organic frameworks centered with lanthanide ions(Ln-MOFs),also known as lanthanide coordination polymers,are considered as a charming category of multi-functional hybrid crystalline materials with fascinating structures and incomparable luminescent characteristics.Benefited from their unique merits,Ln-MOFs have been largely developed as excellent luminescent sensors for fast and efficient sensing various analytes.In this review,we aim to introduce some of the recent researches between 2018 to 2022 on Ln-MOFs applied as chemical sensors for ions,antibiotics and amino acids based on luminescent quenching and enhancing effects,and provide an update and summary for the latest progresses in this field. | Yan Yang Shuting Xu Yanli Gai Bo Zhang Lian Chen | 2022 | Chinese Journal of Structural Chemistry2022，41，11： | 2 |
| 14 | A new multi-scale analytic algorithm for edge extraction of strawberry leaf images in natural light显示文摘In this study,a new algorithm was proposed for edge extraction of greenhouse strawberry leaf in natural light based on the 4-level daubechies 5(‘db5’)wavelet decomposition.This algorithm adopts different segmentation methods for the reconstructed images at different scales to erase the external background and the internal leaf vein interference.There were two advantages of this method.One was that it can provide the abstraction from different spaces to express a same image.The other one was that some image features are hard to be acquired in some scale spaces,while the features are easy to be obtained in other scale spaces.In this image process methods,the Otsu threshold segmentation was to obtain the binary image areas,and the Canny segmentation is to obtain the accurate gradient edges,then the morphological methods and the logical calculus methods were to avoid the fragments inside the leaf area and the adhesions outside the leaf area.Since the strawberry leaf images were different respectively,and the greenhouse optical radiation and reflection may cause local non-uniform illumination of leaf image,the pseudo canny edges of leaf image ere divided into three categories in this research.The first category was the external pseudo canny edges area of the first layer reconstructed leaf image,the second category was the internal pseudo canny edges area in highlight of the third layer reconstructed leaf image,the third category was the internal pseudo canny edges area of significantly different grayscale of the third layer reconstructed leaf image.The different processing methods were constructed for the three kinds of different texture features based on the multi scale reconstructed images,then the complete and the accurate leaf edges without interference were obtained.Finally,the multi scale method was simplified and a remarkably effective segmentation algorithm was deduced for the greenhouse strawberry leaf in natural light. | Wang Jianlun Han Yu Zhao Shuangshuang Zheng Hongxu He Can Cui Xiaoying Xu Yun Chen Jianshu Wang Shuting | 2016 | International Journal of Agricultural and Biological Engineering2016，9，1： | 2 |
| 15 | Genomic evidence of lung carcinogenesis associated with coal smoke in Xuanwei area, China显示文摘Xuanwei area, in southwestern China,harbors the highest female lung cancer rate in the country(Supplementary Table 1) [1]. Epidemiological studies have shown that lung cancer incidence in Xuanwei area is associated with the use of different types of local coal for household cooking and heating [2]. The genomic landscape of Xuanwei female adenocarcinoma(XWFA), the most distinctive feature of lung cancer in Xuanwei, has yet to be elucidated systematically. Here,we provide the fundamental resource of genomic datasets for further exploration of XWFA molecular mechanisms and development of targetable therapy. | Honglei Zhang Chao Liu Li Li Xu Feng Qing Wang Jihua Li Shaobin Xu Shuting Wang Qianlu Yang Zhenghai Shen Jinhua Su Xiaosan Su Ruifen Sun Xuhong Zhou Junliang Wang Yongchun Zhou Baowei Jiao Wanbao Ding Xianbao Cao Yue Wang Yunchao Huang Lianhua Ye | 2021 | National Science Review2021，8，12： | 2 |
| 16 | RNA Methylome Reveals the m^(6)A-mediated Regulation of Flavor Metabolites in Tea Leaves under Solar-withering显示文摘The epitranscriptomic mark N6-methyladenosine(m^(6)A),which is the predominant internal modification in RNA,is important for plant responses to diverse stresses.Multiple environmental stresses caused by the tea-withering process can greatly influence the accumulation of specialized metabolites and the formation of tea flavor.However,the effects of the m^(6)A-mediated regulatory mechanism on flavor-related metabolic pathways in tea leaves remain relatively uncharacterized.We performed an integrated RNA methylome and transcriptome analysis to explore the m^(6)Amediated regulatory mechanism and its effects on flavonoid and terpenoid metabolism in tea(Camellia sinensis)leaves under solar-withering conditions.Dynamic changes in global m^(6)A level in tea leaves were mainly controlled by two m^(6)A erasers(CsALKBH4A and CsALKBH4B)during solar-withering treatments.Differentially methylated peak-associated genes following solarwithering treatments with different shading rates were assigned to terpenoid biosynthesis and spliceosome pathways.Further analyses indicated that CsALKBH4-driven RNA demethylation can directly affect the accumulation of volatile terpenoids by mediating the stability and abundance of terpenoid biosynthesis-related transcripts and also indirectly influence the flavonoid,catechin,and theaflavin contents by triggering alternative splicing-mediated regulation.Our findings revealed a novel layer of epitranscriptomic gene regulation in tea flavor-related metabolic pathways and established a link between the m^(6)A-mediated regulatory mechanism and the formation of tea flavor under solar-withering conditions. | Chen Zhu Shuting Zhang Chengzhe Zhou Caiyun Tian Biying Shi Kai Xu Linjie Huang Yun Sun Yuling Lin Zhongxiong Lai Yuqiong Guo | 2023 | Genomics, Proteomics & Bioinformatics2023，21，4： | 2 |
| 17 | Occurrence and distribution of vibrios in fishes and shellfishes in coastal waters of Hong Kong显示文摘The summer occurrence and distribution of vibrios in the fishes and shellfishes in the coasal waters of Hong Kong were investigaed. A total of 69 strains of vibrios were isolated from all samples examined. The strains, along with 10 reference strains were classified with the technique of numerical taxonomy bared on 54 characters and 62 of the 69 strains fell into 5 major phena, identified as V. paraheamolyticus (30 strains), V. alginoloticus (23 strains), V. choloerae (3 strains), V. harveyi (2 strains) and V. fluinalis (4 strains). Among them, V.paraheamolyticus and V. alginolyticus were the predominant species in the fishes, shellfishes and the coastal waters of Hong Kong and comprised 43. 5 % and 33. 3 % of the total Vibrio spp. isolates respectively. Meanwhile, 3 strains of non-Ol V. cholerae were isolated from oyster and it was the first time to record V. cholerae non-Ol in seawater or from shellfishes in Hong Kong. These results highlighted the potential risks of food poisoning associated with raw or undercooked seafood. | Xu Huaishu, Li Jun, (College of Marine Life Sciences, Ocean University of Qingdao, Qingdao 266003, China)Phyllis Brayton, Rita R. Colwell, David Swartz, (University of Maryland, College Park, 20742, USA)Norman Y. S. Woo, Zhang Shuting (Department of Biol | 1998 | Acta Oceanologica Sinica1998，17，4： | 2 |
| 18 | Occurrence and impact of polychlorinated dibenzo-p-dioxins/dibenzofurans in the air and soil around a municipal solid waste incinerator显示文摘To assess the influence of polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans(PCDD/Fs) on the environment in the vicinity of municipal solid waste incinerators(MSWIs), we determined the levels of PCDD/Fs in air and soil samples collected around a MSWI, which is the largest in China. The International Toxicity Equivalency Quantity(I-TEQ) concentrations of PCDD/Fs in air samples were from 0.0300 to 1.03 pg I-TEQ/m^3(0.445–13.6 pg/m^3), with an average of 0.237 pg I-TEQ/m^3, while in soil samples they ranged from 0.520 to 3.40 pg I-TEQ/g(2.41–88.7 pg/g) with an average of1.49 pg I-TEQ/g. The concentrations of PCDD/Fs in air and soil samples were comparable to other areas, and Pe CDFs were the dominant contributors, which was different from stack gas homologue patterns. Multivariate statistical analysis showed that PCDD/Fs emission from the MSWI did not directly affect the profiles of PCDD/Fs in air and soils, so that vehicles and unidentified emission sources should be considered. The daily inhalation levels of PCDD/Fs for children(0.0110 to 0.392 pg I-TEQ/(kg·day) and adults(0.00600 to 0.221 pg I-TEQ/(kg·day) near the MSWI were lower than the tolerable daily intake of 1.00 to 4.00 pg WHO-TEQ/(kg·day), but in winter the values were higher than in summer. These results can be used as basic data for assessing the risk of PCDD/Fs exposure in residents living around this MSWI, and more monitoring programs and studies should be carried out around MSWIs. | Zhiguang Zhou Yue Ren Jiazhi Chu Nan Li Sen Zhen Hu Zhao Shuang Fan Hui Zhang Pengjun Xu Li Qi Shuting Liang Bin Zhao | 2016 | Journal of Environmental Sciences2016，28，6： | 1 |
| 19 | Fast parallel molecular algorithms for DNA-based computation: Solving the elliptic curve discrete logarithm problem over GF(2^n) 显示文摘 | Li Kenli Zou Shuting Xu Jin | 2008 | Journal of Biomedicine and Biotechnology2008，，1： | 1 |
| 20 | A Strategy for Searching Antigenic Regions in the SARS-CoV Spike Protein显示文摘In the face of the worldwide threat of severe acute respiratory syndrome (SARS)to human life, some of the most urgent challenges are to develop fast and accurate analytical methods for early diagnosis of this disease as well as to create a safe anti-viral vaccine for prevention. To these ends, we investigated the antigenicity of the spike protein (S protein), a major structural protein in the SARS-coronavirus (SARS-CoV). Based upon the theoretical analysis for hydrophobicity of the S protein, 18 peptides were synthesized. Using Enzyme-Linked Immunosorbent Assay (ELISA), these peptides were screened in the sera from SARS patients. According to these results, two fragments of the S gene were amplified by PCR and cloned into pET-32a. Both S fragments were expressed in the BL-21 strain and further purified with an affinity chromatography. These recombinant S fragments were confirmed to have positive cross-reactions with SARS sera, either by Western blot or by ELISA. Our results demonstrated that the potential epitope regions were located at Codons 469-882 in the S protein, and one epitope site was located at Codons 599-620. Identification of antigenic regions in the SARS-CoV S protein may be important for the functional studies of this virus or the development of clinical diagnosis. | Yan Ren, Zhengfeng Zhou, Jinxiu Liu, Liang Lin, Shuting Li, Hao Wang, Ji Xia, Zhe Zhao, Jie Wen, Cuiqi Zhou, Jingqiang Wang, Jianning Yin, Ningzhi Xu, and Siqi LiuBeijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China & Beijing Proteomics Institute, Beijing 101300, China. | 2003 | Genomics, Proteomics & Bioinformatics2003，1，3： | 1 |
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