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| 1 | Environmental impacts on the distribution of microbial tetraether lipids in Chinese lakes with contrasting pH: Implications for lacustrine paleoenvironmental reconstructions显示文摘Glycerol dialkyl glycerol tetraethers(GDGTs) in lake sediments are useful biomarkers for the continental paleoclimatic reconstruction. However, the environmental controls on the distribution of these compounds, in particular the 6-methyl isomers of bacterial branched GDGTs(bGDGTs), in the lakes with contrasting pH, are still unknown, hindering their application for paleo-reconstructions. Here, we investigated the environmental impacts on the distribution of GDGTs in 17 alkaline lakes and 1 acid lake in China. It was found that the dissolved oxygen content in water column may have an impact on the distribution of archaeal isoprenoid GDGTs(iGDGTs) by causing the change in archaeal communities. The ratio of GDGT-0/crenarchaeol increases with decreasing oxygen content, indicating that the relative abundance of anaerobic methanogenic archaea or Miscellaneous Crenarchaeotic Group(MCG) vs. aerobic Thaumarchaeota is controlled by the oxygen content dissolved in water of these lakes. Thaumarchaeota are likely to contribute only a small proportion of iGDGTs in the relatively oxygen-depleted lakes, and thus TEX_(86) is not suitable for the reconstruction of the surface temperature of these lakes. The abundance ratio of iGDGTs to bGDGTs(R_(i/b)) appears to show no relationship with water pH in all the lakes, but exhibits a significant positive correlation with the water depth of the acid Lake Qinghai in Tengchong. As expected, the methylation degree of bGDGTs(MBT′) was found to correlate with both mean annual air temperature(MAT) and water pH, and the cyclization degree of bGDGTs(CBT) correlates only with water pH in these lakes. However, the MBT′_(5ME), an index to measure the methylation degree of 5-methyl bGDGTs, exhibits no relationship with MAT, whereas MBT′_(6ME), the methylation degree of 6-methyl bGDGTs, was found to correlate significantly with MAT. This is opposite to the situation observed in the global soils, pointing to a different adaptation of b GDGT-producing bacteria to environmental variables or different microbial sources of bGDGTs in these lakes. The relative abundance of 6- vs. 5-methyl bGDGTs is controlled by pH in these lakes, similar to that observed in worldwide soils. Hence, the isomer ratio(IR) of 6-methyl bGDGTs or CBT′ can be used as a proxy for water pH, although they might be influenced by other environmental factors including temperature in the lakes with a narrow range of pH. | DANG XinYue XUE JianTao YANG Huan XIE ShuCheng | 2016 | Science China Earth Sciences2016，59，5： | 9 |
| 2 | A Polysaccharide Isolated from the Medicinal Herb Bletilla striata Induces Endothelial Cells Proliferation and Vascular Endothelial Growth Factor Expression in vitro显示文摘 | Chunming Wang Jiantao Sun Yi Luo Weihua Xue Huajia Diao Lei Dong Jiangning Chen Junfeng Zhang | 2006 | Biotechnology Letters2006，，8： | 1 |
| 3 | Long chain n-alkanes and their carbon isotopes in lichen species from western Hubei Province: implication for geological records显示文摘五件 coticolous 地衣样品从中国的西方的湖北省被收集分析长链 n 链烷和他们的碳同位素作文。在到有在 C ^ 潜水艇 21 ^ 和 C ^ 潜水艇之间的强壮的 odd-over-even 优势的 C ^ 潜水艇 33 ^ 的从 C ^ 潜水艇 17 ^ 的碳数字的 n 链烷范围 33 ^ 。地衣被 n-C ^ 潜水艇 29 ^ 在 Dajiuhu 的样品统治, Shennongjia 山,但是由两个 n-C ^ 代用品, 23 ^ 和 n-C ^ 在 Qizimei 代替 29 ^ 山。这差别可以从不同环境条件导致这二个地点。^ 啜在地衣样品的长链 n 链烷的 13 ^ C 价值显示出 C3 植物的签名。把同位素的价值和以前的结果基于 compoundspecific 碳,我们说那链烷相当或相同的事物 > C ^ 潜水艇 23 ^ 主要从共生真菌发源,当时共生水藻仅仅贡献长链链烷的踪迹数量。伟人有趣是在 Qizimei 样品的长链 3-methylalkanes 的出现。这些 anteiso 混合物从 C ^ 潜水艇 24 ^到 C ^ 潜水艇 32 ^ ,显示明显的 even-over-odd 优势。这研究表明有 n-C ^ 潜水艇 23 ^ 链烷的长链 3-methylalkanes 的协会可能被用作代理重建地球历史上的地衣的 paleoecological 含意[出版摘要] | Xianyu HUANG Jiantao XUE Shouyu GUO | 2012 | Frontiers of Earth Science2012，6，1： | 1 |
| 4 | Synthesis and self-assembly of amphiphilic poly(acrylic acid-b-D,L- lactide) to form micelles for pH-responsive drug delivery显示文摘 | Xue Yanan Huang Zhenzhen Zhang Jiantao | 2009 | Polymer2009，50，15： | 1 |
| 5 | Fabrication of high quality strained SiGe on Si substrate by RPCVD显示文摘In this study,the growth kinetics of SiGe in a reduced pressure chemical vapor deposition system using dichlorosilane(SiH2Cl2) and germane(GeH4) as the Si and Ge precursors were investigated.The SiGe growth rate and Ge content were found to depend on the deposition temperature,GeH4 flow and reactor chamber pressure.The SiGe growth rate escalates with increasing deposition temperature,while the Ge content is reduced.The SiGe growth rate accelerates with increasing GeH4 flow,while the Ge content increases more slowly.According to the experimental data,a new relationship between Ge content(x) and F(GeH4)/F(SiH2Cl2) mass flow ratio is deduced:x2.5/(1x) = nF(GeH4)/F(SiH2Cl2).The SiGe growth rate and Ge content improve with increasing reactor chamber pressure.By selecting proper precursor flows and reactor pressure,SiGe films with the same Ge content can be fabricated at various temperatures.However,the quality of the SiGe crystals is clearly dependent on the deposition temperature.At lower deposition temperature,higher crystalline quality is achieved.Because the growth rate dramatically drops with lower temperatures,the optimum growth temperature must be a compromise between the crystalline quality and the growth rate.X-ray diffraction,Raman scattering spectroscopy and atomic force microscopy results indicate that 650°C is the optimum temperature for fabrication of Si0.75Ge0.25 film. | XUE ZhongYing CHEN Da LIU LinJie JIANG HaiTao BIAN JianTao WEI Xing DI ZengFeng ZHANG Miao WANG Xi | 2012 | Chinese Science Bulletin2012，57，15： | 1 |
| 6 | A Polysaccharide Isolated from the Medicinal Herb Bletilla striata Induces Endothelial Cells Proliferation and Vascular Endothelial Growth Factor Expression in vitro显示文摘 | Chunming Wang Jiantao Sun Yi Luo Weihua Xue Huajia Diao Lei Dong Jiangning Chen Junfeng Zhang | 2006 | Biotechnology Letters2006，，8： | 1 |
| 7 | Management of Lisfranc injury with anterolateral calcaneal compression fracture显示文摘To the Editor:The mechanism of Lisfranc injury is complex and may cause different patterns of fractures or dislocation of the midfoot complex.Abduction force injury is a special injury pattern,which may cause the fracture or(and)dislocation of the lateral column of the Lisfranc joint,especially the cuboid.[1,2,3]However,the anterolateral calcaneus is less commonly involved.Since the fragment of the calcaneocuboid(CC)joint facet is too uncon-spicuous to manifest on a plain X-ray,and surgeons always pay more attention to the Lisfranc joint,anterolateral calcaneal compression fracture and even Chopart joint injuries are often overlooked,which may lead to a delayed surgical management,chronic foot deformities,and functional disability. | Wenqi Gu Rui Zhang Wanjun Liu Zhongmin Shi Guohua Mei Jianfeng Xue Jian Zou Xiaokang Wang Jiantao Jiang | 2022 | Chinese Medical Journal2022，，6： | 1 |
| 8 | STI PCR: An efficient method for amplification and de novo synthesis of long DNA sequences显示文摘Despite continuous improvements,it is difficult to efficiently amplify large sequences from complex templates using current PCR methods.Here,we developed a suppression thermo-interlaced(STI)PCR method for the efficient and specific amplification of long DNA sequences from genomes and synthetic DNA pools.This method uses site-specific primers containing a common 50 tag to generate a stem-loop structure,thereby repressing the amplification of smaller non-specific products through PCR suppression(PS).However,large target products are less affected by PS and show enhanced amplification when the competitive amplification of non-specific products is suppressed.Furthermore,this method uses nested thermointerlaced cycling with varied temperatures to optimize strand extension of long sequences with an uneven GC distribution.The combination of these two factors in STI PCR produces a multiplier effect,markedly increasing specificity and amplification capacity.We also developed a webtool,calGC,for analyzing the GC distribution of target DNA sequences and selecting suitable thermo-cycling programs for STI PCR.Using this method,we stably amplified very long genomic fragments(up to 38 kb)from plants and human and greatly increased the length of de novo DNA synthesis,which has many applications such as cloning,expression,and targeted genomic sequencing.Our method greatly extends PCR capacity and has great potential for use in biological fields. | Zhe Zhao Xianrong Xie Weizhi Liu Jingjing Huang Jiantao Tan Haixin Yu Wubei Zong Jintao Tang Yanchang Zhao Yang Xue Zhizhan Chu Letian Chen Yao-Guang Liu | 2022 | Molecular Plant2022，15，4： | 1 |
| 9 | Occurrence of diploptene in moss species from the Dajiuhu peatland in Southern China显示文摘 | Huang Xianyu Wang Canfa Xue Jiantao | | 0，，03： | 1 |
| 10 | Main environmental drivers of abundance,diversity and community structure of comammox Nitrospira in paddy soils显示文摘The recently discovered complete ammonia oxidizers comammox Nitrospira contain clades A and B that can establish an independent one-step nitrification process;however,little is known about their environmental drivers or habitat distributions in agricultural soils.Previous studies on comammox Nitrospira in paddy soils have mainly focused on small-scale samples,and there is a lack of multisite research on comammox Nitrospira in paddy soils.In this study,we conducted a survey of 36 paddy soils to understand the community structure,abundance,and diversity of comammox Nitrospira and the degree to which they are affected by environmental factors at a large scale.Comammox Nitrospira were found to be widely distributed among the paddy soils.The abundance of comammox Nitrospira clade A was mostly lower than that of clade B,whereas its diversity was mostly higher than that of clade B.Correlation analysis showed that multiple factors affected(P<0.05)the abundance of comammox Nitrospira,including soil pH,organic matter,total carbon,and total nitrogen,latitude,mean annual temperature,and mean annual precipitation.Moreover,there was a clear relationship between the comammox Nitrospira community and habitat,indicating that some amplicon sequence variants(ASVs)had a unique dominant position in specific habitats.Phylogenetic analysis showed that the ASVs of comammox Nitrospira clade A clustered with the known sequences in the paddy soils and were significantly different from the known sequences in other habitats,which may be related to the unique paddy field habitat.In contrast,comammox Nitrospira clade B showed no clear habitat dependence.These results support the wide distribution and high abundance of comammox Nitrospira in paddy soils and provide novel insights into nitrogen cycling and nutrient management in agricultural ecosystems. | Fuyun GAO Yaying LI Haoxin FAN Jiantao XUE Huaiying YAO | 2023 | Pedosphere2023，33，5： | 0 |
| 11 | Seasonal variations of leaf wax n-alkane distributions and δ^(2) H values in peat-forming vascular plants from the Dajiuhu peatland, central China显示文摘Leaf wax n-alkane compositions have been widely applied to reconstruct paleoclimate histories in peat deposits,yet understanding of how the n-alkanes vary during seasonal plant growth remains limited.Here we report variations in the molecular and wax-derived nalkane hydrogen isotope(δ^(2) Halk)in the three dominant vascular plant species(Sanguisorba officinalis,Carex argyi,Euphorbia esula)and surface peat deposits nearby from the Dajiuhu peatland over a growing season.All three species show a relatively high carbon preference index(CPI)in the beginning of the growing season,with the CPI values reaching as high as 50 in two of the three species.Two species(S.officinalis,E.esula)display relatively stable average chain length(ACL)values over the four sampling intervals,with standard derivations of 0.2-0.3.In contrast,C.argyi exhibits a significant fluctuation of ACL values(averaging 28.11.4)over the growing season.Theδ^(2) Halk in all three species decreased during leaf growth.In the final stage of growth,theδ^(2) Halk values of the three species are similar to those in the surface peats collected from the peatland.Combining the results of our measurements of alkane concentration andδ^(2) H values,it is likely that de novo synthesis of leaf wax n-alkanes in the peatforming plant species is mainly at the early stage of leaf development.In the following months,the removal process exceeds renewal,resulting in a general decrease of the concentration of the total n-alkanes and the integratedδ^(2) Halk values.Thus theδ^(2) Halk values probably integrate the environmental variations at the end of the plant growth period rather than the whole period or the early growth period.These results are significant and have the potential to improve the utility ofδ^(2) Halk values in paleoenvironmental reconstructions. | Jiantao XUE Xueying ZHANG Jinzhi LI Zhiqi ZHANG Huaiying YAO | 2022 | Frontiers of Earth Science2022，16，3： | 0 |
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