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| 1 | Development of "Purple Endosperm Rice" by Engineering Anthocyanin Biosynthesis in the Endosperm with a High-Efficiency Transgene Stacking System显示文摘Anthocyanins 在主食庄稼的 anthocyanin 生合成的高抗氧化剂活动,和工程例如米饭(Oryza sativa L.) ,能为改进人的健康提供支持健康的食物。然而,为 biofortification 设计新陈代谢的小径仍然保持困难,并且因为它的 biosynthetic 小径的复杂基因规章的网络,在米饭内乳设计 anthocyanin 生产的以前的尝试失败了。在这研究,我们为叠的 transgene 开发了一个高效率的向量系统并且使用了它在米饭内乳设计 anthocyanin 生合成。我们做了包含内乳特定的倡导者驾驶的八 anthocyanin 相关的基因(从从薄荷科植物的玉米和六结构的基因 ?的二规章的基因) 的构造,加为标记切除的一个可选的标记和基因。有这构造的米饭的转变产生了新奇 biofortified germplasm 紫色内乳瑞斯(用汉语的叫的 Zijingmi ) ,它在内乳有高 anthocyanin 内容和抗氧化剂活动。这 anthocyanin 生产源于 transgenes 和产生激活的表示(或改进) 13 内长的 anthocyanin 生合成基因的表示,那是 silenced 或在野类型的米饭内乳在底层表示。这研究为叠的 transgene 提供一个有效、万用的工具箱并且为一条复杂生物小径的成功的工程表明它的使用,建议为在工厂的农学的特点的合成生物学和改进的这个工具箱的潜在的用途。 | Qinlong Zhu Suize Yu Dongchang Zeng Hongmei Liu Huicong Wang Zhongtang Yang Xianrong Xie Rongxin Shen Jiantao Tan Heying Li Xiucai Zhao Qunyu Zhang Yuanling Chen Jingxing Guo Letian Chen Yao-Guang Liu | 2017 | Molecular Plant2017，10，7： | 47 |
| 2 | A modified high-efficiency thermal asymmetric interlaced PCR method for amplifying long unknown flanking sequences显示文摘Rapid and efficient isolation of unknown flanking DNA sequences adjacent to known regions is important for molecular biology research.For this purpose,several PCR-based methods have been reported,including inverse PCR(Uchiyama and Watanabe,2006),ligation-mediated PCR(Yan et al.,2003;Ballester et al.,2005;Wang et al.,2007;Trinh et al.,2012)and randomly primed PCR(Liu and Whittier,1995;Liu et al.,1995;Antal et al. | Jiantao Tan Qi Gong Suize Yu Yuke Hou Dongchang Zeng Qinlong Zhu Yao-Guang Liu | 2019 | Journal of Genetics and Genomics2019，46，7： | 7 |
| 3 | PhieCBEs:Plant High-Efficiency Cytidine Base Editors with Expanded Target Range显示文摘Clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9(Cas9)-mediated genome editing can efficiently produce gene-knockout mutants.On the other hand,CRISPR/Cas-derived base editors offer the ability to induce precise nucleotide substitutions(Komor et al.,2016).Cytidine base editors(CBEs)consist of a cytidine deaminase fused with a Cas9-nickase variant(Cas9n,with a D10A substitu-tion)and can achieve site-specific C-to-T substitution.Similarly,adenine base editors use an adenine deaminase forA-to-G substi-tution.These systems have been used in various organisms(Mishra et al.,2019).However,the Cas9 complex requires target sites containing NGG protospacer adjacent motifs(PAMs),thus restricting selection of potential targets.A number of CBEs have been developed using Cas9 variants(mostly Cas9n),cytidine deaminases(such as rAPOBEC1 and PmCDA1),and uracil glycosylase inhibitor(UGI)domains.These CBEs of the first generation(BE1,rAPOBEC1-dCas9),second generation(BE2,rAPOBEC1-dCas9-UGI),and third generation(BE3,rAPOBEC1-Cas9n-UGI)have moderate editing efficiencies in mammalians(Komor etal.,2016). | Dongchang Zeng Taoli Liu Jiantao Tan Yaling Zhang Zhiye Zheng Bin Wang Degui Zhou Xianrong Xie Menghui Guo Yao-Guang Liu Qinlong Zhu | 2020 | Molecular Plant2020，13，12： | 5 |
| 4 | Modification of polarization filtering technique in HF ground wave radar显示文摘The polarization filter using three orthogonal linear polarization antennas can suppress more disturbances than the polarization filter using two orthogonal linear polarization antennas in HF ground wave radar. But the algorithm of the threedimension filter is relatively complicated and not suitable for real-time processing. It can’t use linear and nonlinear polarization vector translation technique directly. A modified polarization filter which is simple and has same suppressing ability as the three-dimension polarization filter is given. It only has half parameters of the primary one. Some problems about estimation of polarization parameters and selection of disturbances are discussed. A method of holding the phase of radar backscatter signal constantly is put forward so that unstationary disturbance signal can be processed. | Zhang Guoyi Tan Zhongji Wang Jiantao | 2006 | Journal of Systems Engineering and Electronics2006，17，4： | 3 |
| 5 | TIR/BB-loop mimetic AS-1 protects vascular endothelial cells from injury induced by hypoxia/reoxygenation显示文摘Morphological and functional abnormalities of vascular endothelial cells(VECs) are risk factors of ischemiareperfusion in skin flaps.Signaling pathway mediated by interleukin-1 receptor(IL-1 R) is essential to hypoxia/reoxygenation(H/R) injury of VECs.While the TIR/BB-loop mimetic(AS-1) disrupts the interaction between IL-1 R and myeloid differentiation primary-response protein 88(MyD88),its role in the VECs dysfunction under H/R is unclear.In this study,we first showed that there was an infiltration of inflammatory cells and the apoptosis of VECs by using a skin flap section from patients who received flap transplantation.We then showed that the H/R treatment induced apoptosis and loss of cell migration of endothelial cell line H926 were attenuated by AS-1.Furthermore,our data suggested that AS-1 inhibits the interaction between IL-1 R and MyD88,and subsequent phosphorylation of IκB and p38 pathway,as well as the nuclear localization of NF-κB subunit p65/p50.Thus,this study indicated that the protective role of AS-1 in H/R induced cellular injury may be due to the AS-1 mediated down-regulation of IL-1 R signaling pathway. | Zhijia Zhang Yuxing Hou Jiantao Li Chao Tang Linli Que Qian Tan Yuehua Li | 2020 | The Journal of Biomedical Research2020，34，5： | 2 |
| 6 | Plant Synthetic Metabolic Engineering for Enhancing Crop Nutritional Quality显示文摘Nutrient deficiencies in crops are a serious threat to human health,especially for populations in poor areas.To overcome this problem,the development of crops with nutrient-enhanced traits is imperative.Biofortification of crops to improve nutritional quality helps combat nutrient deficiencies by increasing the levels of specific nutrient components.Compared with agronomic practices and conventional plant breeding,plant metabolic engineering and synthetic biology strategies are more effective and accurate in synthesizing specific micronutrients,phytonutrients,and/or bioactive components in crops.In this review,we discuss recent progress in the field of plant synthetic metabolic engineering,specifically in terms of research strategies of multigene stacking tools and engineering complex metabolic pathways,with a focus on improving traits related to micronutrients,phytonutrients,and bioactive components.Advances and innovations in plant synthetic metabolic engineering would facilitate the development of nutrient-enriched crops to meet the nutritional needs of humans. | Qinlong Zhu Bin Wang Jiantao Tan Taoli Liu Li Li Yao-Guang Liu | 2020 | Plant Communications2020，1，1： | 2 |
| 7 | Hydrogen production by methane cracking over different coal chars显示文摘 | Wei Ling Tan Yisheng Han Yizhuo Zhao Jiantao Wu Jinhu Zhang Dongke | 2011 | Fuel2011，90，11： | 1 |
| 8 | STI PCR: An efficient method for amplification and de novo synthesis of long DNA sequences显示文摘Despite continuous improvements,it is difficult to efficiently amplify large sequences from complex templates using current PCR methods.Here,we developed a suppression thermo-interlaced(STI)PCR method for the efficient and specific amplification of long DNA sequences from genomes and synthetic DNA pools.This method uses site-specific primers containing a common 50 tag to generate a stem-loop structure,thereby repressing the amplification of smaller non-specific products through PCR suppression(PS).However,large target products are less affected by PS and show enhanced amplification when the competitive amplification of non-specific products is suppressed.Furthermore,this method uses nested thermointerlaced cycling with varied temperatures to optimize strand extension of long sequences with an uneven GC distribution.The combination of these two factors in STI PCR produces a multiplier effect,markedly increasing specificity and amplification capacity.We also developed a webtool,calGC,for analyzing the GC distribution of target DNA sequences and selecting suitable thermo-cycling programs for STI PCR.Using this method,we stably amplified very long genomic fragments(up to 38 kb)from plants and human and greatly increased the length of de novo DNA synthesis,which has many applications such as cloning,expression,and targeted genomic sequencing.Our method greatly extends PCR capacity and has great potential for use in biological fields. | Zhe Zhao Xianrong Xie Weizhi Liu Jingjing Huang Jiantao Tan Haixin Yu Wubei Zong Jintao Tang Yanchang Zhao Yang Xue Zhizhan Chu Letian Chen Yao-Guang Liu | 2022 | Molecular Plant2022，15，4： | 1 |
| 9 | Fuzzy Adaptive Control of Muhivariable Nonlinear Systems显示文摘 | Tong Shaocheng Tang Jiantao Wang tan | 2000 | Fuzzy Sets and Systems2000，111，2： | 1 |
| 10 | Overexpression of maize GOLDEN2 in rice and maize calli improves regeneration by activating chloroplast development显示文摘Golden2(G2), a member of the GARP transcription factor superfamily, regulates several biological processes and phytohormone signaling pathways in plants. In this study, we used a rice codon-optimized maize G2 gene(rZmG2) to improve the regeneration efficiency of rice and maize calli for genetic transformation. We isolated a promoter driving strong and callus-specific expression from rice to drive rZmG2 transcription from a transgene after transformation of two indica and two japonica rice cultivars. The resulting rZmG2 transgenic calli turned green in advance at the differentiation stage, thus significantly raising the regeneration rates of the transgenic indica and japonica rice plants relative to control transformations. Similar effect of this gene on improving maize transformation was also observed. Transcriptome sequencing and RT-qPCR analyses showed that many rice genes related to chloroplast development and phytohormones are upregulated in rZmG2-transgenic calli. These results demonstrate that rZmG2 can promote embryogenic callus differentiation and improve regeneration efficiency by activating chloroplast development and phytohormone pathways. We also established a heat-inducible Cre/loxP-based gene-excision system to remove rZmG2 and the antibiotic selectable gene after obtaining the transgenic plants. This study provides a useful tool for functional genomics work and biotechnology in plants. | Wanni Luo Jiantao Tan Tie Li Ziting Feng Zhi Ding Xianrong Xie Yuanling Chen Letian Chen Yao-Guang Liu Qinlong Zhu Jinxing Guo | 2023 | Science China(Life Sciences)2023，66，2： | 0 |
| 11 | Rice OsUBR7 modulates plant height by regulating histone H2B monoubiquitination and cell proliferation显示文摘Plant height is an important agronomic trait for lodging resistance and yield.Here,we report a new plantheight-related gene,OsUBR7 in rice(Oryza sativa L.);knockout of OsUBR7 caused fewer cells in internodes,resulting in a semi-dwarf phenotype.OsUBR7 encodes a putative E3 ligase containing a plant homeodomain finger and a ubiquitin protein ligase E3 component N-recognin 7(UBR7)domain.OsUBR7 interacts with histones and monoubiquitinates H2B(H2Bub1)at lysine148 in coordination with the E2 conjugase OsUBC18.OsUBR7 mediates H2Bub1 at a number of chromatin loci for the normal expression of target genes,including cell-cycle-related and pleiotropic genes,consistent with the observation that cell-cycle progression was suppressed in the osubr7 mutant owing to reductions in H2Bub1 and expression levels at these loci.The genetic divergence of OsUBR7 alleles among japonica and indica cultivars affects their transcriptional activity,and these alleles may have undergone selection during rice domestication.Overall,our results reveal a novel mechanism that mediates H2Bub1 in plants,and UBR7 orthologs could be utilized as an untapped epigenetic resource for crop improvement. | Yangyi Zheng Sensen Zhang Yanqiu Luo Fuquan Li Jiantao Tan Bin Wang Zhe Zhao Huifang Lin Tingting Zhang Jianhong Liu Xupeng Liu Jingxin Guo Xianrong Xie Letian Chen Yao-Guang Liu Zhizhan Chu | 2022 | Plant Communications2022，3，6： | 0 |
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