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| 1 | Optimal illumination scheme for isotropic quantitative differential phase contrast microscopy显示文摘Differential phase contrast microscopy(DPC) provides high-resolution quantitative phase distribution of thin transparent samples under multi-axis asymmetric illuminations. Typically, illumination in DPC microscopic systems is designed with two-axis half-circle amplitude patterns, which, however, result in a non-isotropic phase contrast transfer function(PTF). Efforts have been made to achieve isotropic DPC by replacing the conventional half-circle illumination aperture with radially asymmetric patterns with three-axis illumination or gradient amplitude patterns with two-axis illumination. Nevertheless, the underlying theoretical mechanism of isotropic PTF has not been explored, and thus, the optimal illumination scheme cannot be determined. Furthermore, the frequency responses of the PTFs under these engineered illuminations have not been fully optimized, leading to suboptimal phase contrast and signal-to-noise ratio for phase reconstruction. In this paper, we provide a rigorous theoretical analysis about the necessary and sufficient conditions for DPC to achieve isotropic PTF. In addition,we derive the optimal illumination scheme to maximize the frequency response for both low and high frequencies(from 0 to 2 NAobj) and meanwhile achieve perfectly isotropic PTF with only two-axis intensity measurements.We present the derivation, implementation, simulation, and experimental results demonstrating the superiority of our method over existing illumination schemes in both the phase reconstruction accuracy and noise-robustness. | YAO FAN JIASONG SUN QIAN CHEN XIANGPENG PAN LEI TIAN CHAO ZUO | 2019 | Photonics Research2019，7，8： | 8 |
| 2 | The complete reference genome for grapevine (Vitis vinifera L.) genetics and breeding显示文摘Grapevine is one of the most economically important crops worldwide.However,the previous versions of the grapevine reference genome tipically consist of thousands of fragments with missing centromeres and telomeres,limiting the accessibility of the repetitive sequences,the centromeric and telomeric regions,and the study of inheritance of important agronomic traits in these regions.Here,we assembled a telomere-to-telomere(T2T)gap-free reference genome for the cultivar PN40024 using PacBio HiFi long reads.The T2T reference genome(PN_T2T)is 69 Mb longer with 9018 more genes identified than the 12X.v0 version.We annotated 67%repetitive sequences,19 centromeres and 36 telomeres,and incorporated gene annotations of previous versions into the PN_T2T assembly.We detected a total of 377 gene clusters,which showed associations with complex traits,such as aroma and disease resistance.Even though PN40024 derives from nine generations of selfing,we still found nine genomic hotspots of heterozygous sites associated with biological processes,such as the oxidation–reduction process and protein phosphorylation.The fully annotated complete reference genome therefore constitutes an important resource for grapevine genetic studies and breeding programs. | Xiaoya Shi Shuo Cao Xu Wang Siyang Huang Yue Wang Zhongjie Liu Wenwen Liu Xiangpeng Leng Yanling Peng Nan Wang Yiwen Wang Zhiyao Ma Xiaodong Xu Fan Zhang Hui Xue Haixia Zhong Yi Wang Kekun Zhang Amandine Velt Komlan Avia Daniela Holtgräwe Jérôme Grimplet JoséTomás Matus Doreen Ware Xinyu Wu Haibo Wang Chonghuai Liu Yuling Fang Camille Rustenholz Zongming Cheng Hua Xiao Yongfeng Zhou | 2023 | Horticulture Research2023，10，5： | 3 |
| 3 | Establishment of customized mouse stem cell lines by sequential nuclear transfer显示文摘治疗学的克隆,胚胎的干细胞(转换字符) 由此从原子转移(NT ) 被导出胚胎,可以在再生药的新时代起一个主要作用。Inthis 学习我们建立了四十原子转移 -- 从不同施主房间类型或段落的 NTembryos 被导出的转换字符(NT 转换字符) 线。我们发现 NT 转换字符能够形成胚胎植物或动物身体。另外, NT 转换字符表示了 pluripotency 干细胞标记试管内并且能区分进胚胎的纸巾体内。从施主房间能形成完整的术语的早经过 R1 的 NT 胚胎开发了小狗,而那些为为实时出生是必要的 reprogramming 从迟了的经过 R1 ES 施主房间失去了潜力。我们随后建立了近来从 NT 胚囊被开发的 sequentialNT-R1-ESC 线经过 R1 转换字符施主。然而,当在他们的早段落用作原子转移施主时,这些 NT-R1-ESC 排队,没能导致实时小狗。这显示用顺序的 NT 转换字符的治疗学的克隆过程不能救居住在以前的施主代的发展缺乏。 | Chunli Zhao Ruqiang Yao Jie Hao Chenhui Ding Yong Fan Xiangpeng Dai Wei Li Tang Hai Zichuan Liu Yang Yu Yingying Wang Xiaojun Hou Weizhi Ji Qi Zhou Alice Jouneau Fanyi Zeng Liu Wang | 2007 | Cell Research2007，17，1： | 1 |
| 4 | Optimization study on the reconstruction and expansion of an underground rail transit center in Shanghai soft ground显示文摘 | Shaoming Liao Yaoyao Fan Zhenhao Shi Wei Shao Xiangpeng Kong | 2013 | Tunnelling and Underground Space Technology incorporating Trenchless Technology Research2013，，： | 1 |
| 5 | A pseudovirus-based method to dynamically mimic SARS-CoV-2-associated cell-to-cell fusion and transmission显示文摘Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),responsible for the COVID-19 pandemic,has caused tremendous global loss and continues to evolve to generate variants.Entry of SARS-CoV-2 into target host cells is primarily mediated by spike(S),which binds to the host receptor hACE2 and initiates virus-cell membrane fusion[1].Most COVID-19 patients show pneumocyte syncytia in the lungs[2]. | Xiangpeng Sheng Yi Yang Fang Zhu Fan Yang Honghua Wang Ronggui Hu | 2023 | Acta Biochimica et Biophysica Sinica2023，55，11： | 0 |
| 6 | Lightning and deep convective activities over the Tibetan Plateau显示文摘Convections over the Tibetan Plateau play a crucial role in the energy and material exchange between the troposphere and stratosphere,impacting the rain-bearing synoptic systems over eastern China.Lightning can be used as a key indicator of strong convection and therefore its characteristics and relationship with convection over the plateau are of great interest to researchers.The panorama of lightning activity over the plateau has been revealed by recent studies using the optical observation data from the satellite. | Dong Zheng Yijun Zhang Yang Zhang Wen Yao Fei Wang Weitao Lyu Qing Meng Xiangpeng Fan Ying Ma Jun Yang | 2020 | National Science Review2020，7，3： | 0 |
| 7 | Host cyclophilin A facilitates SARS-CoV-2 infection by binding and stabilizing spike on virions显示文摘Dear Editor,The novel coronavirus SARS-CoV-2 rapidly evolutes to increase its infectivity and transmissibility,facilitates its immune escape,impairs vaccine efficacy,and currently causes repeated infections in human,which is mainly determined by the accumulated mutations in spike(S).1 Transmembrane S is a heavily glycosylated protein on the surface of the virion as homotrimer,responsible for binding to human angiotensin-converting enzyme 2(hACE2)receptor.2 While the architecture of S trimers has been well studied,how the stability of S is regulated by potential host factors remains unknown. | Xiangpeng Sheng Fang Zhu Hong Peng Fan Yang Yi Yang Cong Yang Zhen Wang Wei Chen Deyin Guo Ronggui Hu | 2024 | Signal Transduction and Targeted Therapy2024，9，1： | 0 |
| 8 | Comparative pluripotency analysis of mouse embryonic stem cells derived from wild-type and infertile hermaphrodite somatic cell nuclear transfer blastocysts显示文摘Therapeutic cloning, whereby embryonic stem cells (ESCs) are derived from patient-specific cloned blastocysts via somatic cell nuclear transfer (SCNT), holds great promise for treating many human diseases using regenerative medicine. Teratoma formation and germline transmission have been used to confirm the pluripotency of mouse stem cells, but human embryonic stem cells (hESCs) have not been proven to be fully pluripotent owing to the ethical impossibility of testing for germ line transmission, which would be the strongest evidence for full pluripotency. Therefore, formation of differentiated cells from the three somatic germ layers within a teratoma is taken as the best indicator of pluripotency in hESC lines. The possibility that these lines lack full multi-or pluripotency has not yet been evaluated. In this study, we established 16 mouse ESC lines, including 3 genetically defective nuclear transfer- ESC (ntESC) lines derived from SCNT blastocysts of infertile hermaphrodite F1 mice and 13 ntESC lines derived from SCNT blastocysts of normal F1 mice. We found that the defective ntESCs expressed all in vitro markers of pluripotency and could form teratomas that included derivatives from all three germ layers, but could not be transmitted via the germ line, in contrast with normal ntESCs. Our results indicate that teratoma formation assays with hESCs might be an insufficient standard to assess full pluripotency, although they do define multipotency to some degree. More rigorous standards are required to assess the safety of hESCs for therapeutic cloning. | FAN Yong TONG Man ZHAO ChunLi DING ChenHui HAO Jie LV Zhuo DAI XiangPeng HAI Tang LI XueMei YAO RuQiang YU Yang LI ZanDong WANG Liu ALICE Jouneau ZHOU Qi | 2008 | Chinese Science Bulletin2008，53，23： | 0 |
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