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| 1 | An increase in corn resistant starch decreases protein fermentation and modulates gut microbiota during in vitro cultivation of pig large intestinal inocula显示文摘High-protein diet could cause an increase in protein fermentation in the large intestine, leading to an increased production of potentially detrimental metabolites. We hypothesized that an increase in corn resistant starch content may attenuate the protein fermentation. The aim of this study was to evaluate the effect of resistant starch on protein fermentation by inocula from large intestine of pigs using in vitro cultivation. Fermentation patterns were analyzed during a 24-h incubation of cecal and colonic digesta with varying corn resistant starch contents, using casein protein as sole nitrogen source. The results showed that the concentration of short-chain fatty acids(SCFA) and cumulative gas production were significantly increased(P < 0.05), while ammonia-nitrogen(NH_3-N) and branched-chain fatty acids(BCFA), which indicated protein fermentation, decreased when the corn resistant starch levels increased(P < 0.05). The copies of total bacteria, Bifidobacterium and Lactobacillus were significantly increased with the increased corn resistant starch levels after incubation(P < 0.05). The copies of the Bifidobacterium and Lactobacillus in cecum were significantly higher than those in colon(P < 0.05). We conclude that the addition of corn resistant starch weakens the protein fermentation by influencing microbial population and reducing protein fermentation in the cecum and colon in vitro. | Xiangyu He Weiwei Sun Ting Ge Chunlong Mu Weiyun Zhu | 2017 | Animal Nutrition2017，，3： | 8 |
| 2 | The effect of increased atmospheric temperature and CO_2 concentration during crop growth on the chemical composition and in vitro rumen fermentation characteristics of wheat straw显示文摘This experiment was c onducted to investigate the effects of increased atmospheric temperature and CO_2concentration during crop growth on the chemical composition and in vitro rumen fermentation characteristics of wheat straw.The field experiment was carried out from November 2012 to June 2013 at Changshu(31°32'93 'N,120°41'88' E) agro-ecological experimental station.A total of three treatments were set.The concentration of CO_2was increased to 500 umol/mol in the first treatment(CO_2 group).The temperature was increased by 2 ℃ in the second treatment(TEM group) and the concentration of CO_2 and temperature were both increased in the third treatment(CO_2 + TEM group).The mean temperature and concentration of CO_2 in control group were 10.5 ℃ and413 umol/mol.At harvesting,the wheat straws were collected and analyzed for chemical composition and in vitro digestibility.Results showed that dry matter was significantly increased in all three treatments.Ether extracts and neutral detergent fiber were significantly increased in TEM and CO_2 + TEM groups.Crude protein was significantly decreased in CO_2 + TEM group.In vitro digestibility analysis of wheat straw revealed that gas production was significantly decreased in CO_2 and CO_2 + TEM groups.Methane production was significantly decreased in TEM and CO_2 + TEM groups.Ammonia nitrogen and microbial crude protein were significantly decreased in all three treatments.Total volatile fatty acids were significantly decreased in CO_2 and CO_2 + TEM groups.In conclusion,the chemical composition of the wheat straw was affected by temperature and CO_2 and the in vitro digestibility of wheat straw was reduced,especially in the combined treatment of temperature and CO_2. | Xiangyu He Yanping Wu Min Cai Chunlong Mu Weihong Luo Yanfen Cheng Weiyun Zhu | 2015 | Journal of Animal Science and Biotechnology2015，6，4： | 1 |
| 3 | TMED10-mediated unconventional secretion of IL-33 regulates intestinal epithelium differentiation and homeostasis显示文摘Dear Editor,Unconventional protein secretion(UcPS)refers to the release of non-signal peptide-containing secretory proteins independent of the ER–Golgi trafficking.1 Although two major pathways including vesicle-dependent and-independent UcPS have been revealed,1 the physiological role of each pathway is largely unclear due to the lack of molecular details.Previously,we identified a translocation pathway termed transmembrane p24 trafficking protein 10(TMED10)-channeled UcPS(THU),which regulates vesicle-dependent UcPS pathway.2,3 In THU,TMED10 is a protein translocator key for mediating secretory cargo translocation into the ER–Golgi intermediate compartment to initiate vesicle-dependent UcPS. | Yang Wang Meimei Huang Xiangyue Mu Wanlu Song Qing Guo Min Zhang Yuan Liu Ye-Guang Chen Liang Ge | 2024 | Cell Research2024，34，3： | 0 |
| 4 | Single-cell transcriptome atlas reveals spatiotemporal developmental trajectories in the basal roots of moso bamboo (Phyllostachys edulis)显示文摘Roots are essential for plant growth and development.Bamboo is a large Poaceae perennial with 1642 species worldwide.However,little is known about the transcriptional atlas that underpins root cell-type differentiation.Here,we set up a modified protocol for protoplast preparation and report single-cell transcriptomes of 14279 filtered single cells derived from the basal root tips of moso bamboo.We identified four cell types and defined new cell-type-specific marker genes for the basal root.We reconstructed the developmental trajectories of the root cap,epidermis,and ground tissues and elucidated critical factors regulating cell fate determination.According to in situ hybridization and pseudotime trajectory analysis,the root cap and epidermis originated from a common initial cell lineage,revealing the particularity of bamboo basal root development.We further identified key regulatory factors for the differentiation of these cells and indicated divergent root developmental pathways between moso bamboo and rice.Additionally,PheWOX13a and PheWOX13b ectopically expressed in Arabidopsis inhibited primary root and lateral root growth and regulated the growth and development of the root cap,which was different from WOX13 orthologs in Arabidopsis.Taken together,our results offer an important resource for investigating the mechanism of root cell differentiation and root system architecture in perennial woody species of Bambusoideae. | Zhanchao Cheng Changhong Mu Xiangyu Li Wenlong Cheng Miaomiao Cai Chongyang Wu Jutang Jiang Hui Fang Yucong Bai Huifang Zheng Ruiman Geng Junlei Xu Yali Xie Yuping Dou Juan Li Shaohua Mu Jian Gao | 2023 | Horticulture Research2023，10，8： | 0 |
| 5 | U-Pb-Hf isotopes and petrogenesis of Late Jurassic akakitic quartz monzodiorite in Xingcheng area,western Liaoning Province显示文摘The petrogenesis and geodynamic setting of the Late Jurassic Baita porphyry quartz monzodiorite in Xingcheng-Liaoxi area provide information for understanding the Mesozoic tectonic evolution of the northeastern North China Craton.In this paper,geochronological,whole-rock geochemical,and in-situ zircon Hf isotopic analyses of Baita porphyry quartz monzodiorite were investigated to constrain the crystallization age and petrogenesis.Zircons exhibit typical oscillatory zoning in cathodoluminescence images and show relatively high Th/U ratios(0.78-1.62),and U-Pb analyses indicate that these rocks were crystallized during the Late Jurassic(159±1 Ma).Geochemically,they are characterized by high contents of SiO 2(65.21wt%-65.31wt%),Al 2O 3(16.29wt%-16.31wt%),Sr(521×10^(-6)-539×10^(-6)),and Sr/Y ratio(45.1-47.8)but low Y(10.9×10^(-6)-12.0×10^(-6)),with obvious adakitic geochemical affinities.These above-mentioned findings,combined with their negativeε_(Hf)(t)values(-21.7 to-20.2),corresponding two-stage model age(T_( DM2))of 2579-2484 Ma,as well as low MgO(1.38wt%-1.39wt%),Cr(18.5×10^(-6)-19.5×10^(-6))and Ni(9.45×10^(-6)-9.46×10^(-6))values,indicate that Baita porphyry quartz monzodiorite may be generated by partial melting of the Neoarchean-Paleoproterozoic thickened basaltic lower crust.Based on the results from this study and pre-vious regional studies,it is concluded that Baita porphyry quartz monzodiorite was spatially related to the westward subduction of the Paleo-Pacific plate. | YAN Xiangyu YANG Debin MU Maosong HAO Leran | 2021 | Global Geology2021，24，3： | 0 |
| 6 | Performance Limit Evaluation Strategy for Automated Driving Systems显示文摘Efficient detection of performance limits is critical to autonomous driving.As autonomous driving is difficult to be realized under complicated scenarios,an improved genetic algorithm-based evolution test is proposed to accelerate the evaluation of performance limits.It conducts crossover operation at all positions and mutation several times to make the high-quality chromosome exist in candidate offspring easily.Then the normal offspring is selected statistically based on the scenario com-plexity,which is designed to measure the difficulty of realizing autonomous driving through the Analytic Hierarchy Process.The benefits of modified cross/mutation operators on the improvement of scenario complexity are analyzed theoretically.Finally,the effectiveness of improved genetic algorithm-based evolution test is validated after being applied to evaluate the collision avoidance performance of an automatic parallel parking system. | Feng Gao Jianwei Mu Xiangyu Han Yiheng Yang Junwu Zhou | 2022 | Automotive Innovation2022，5，1： | 0 |
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