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| 1 | Changes in extreme high-temperature tolerance and activities of antioxidant enzymes of sacred lotus seeds显示文摘Sacred lotus (Nelumbo nucifera Gaertn. ‘Tielian’) seed is long-lived and extremely tolerant of high temperature. Water content of lotus and maize seeds was 0.103 and 0.129 g H2O [g DW] ?1, respectively. Water content, germination percentage and fresh weight of seedlings produced by surviving seeds gradually decreased with increasing treatment time at 100℃. Germination percentage of maize (Zea mays L. ‘Huangbaogu’) seeds was zero after they were treated at 100℃ for 15 min and that of lotus seeds was 13.5% following the treatment at 100℃ for 24 h. The time in which 50% of lotus and maize seeds were killed by 100℃ was about 14.5 h and 6 min, respectively. With increasing treatment time at 100℃, relative electrolyte leakage of lotus axes increased significantly, and total chlorophyll content of lotus axes markedly decreased. When treatment time at 100℃ was less than 12 h, subcellular structure of lotus hypocotyls remained fully intact. When treatment time at 100℃ was more than 12 h, plasmoly-sis gradually occurred, endoplasmic reticulum became unclear, nuclei and nucleoli broke down, most of mitochondria swelled, lipid granules accumulated at the cell periphery, and organelles and plas-molemma collapsed. Malondialdehyde (MDA) content of lotus axes and cotyledons decreased during 0-12 h of the treatment at 100℃ and then increased. By contrast, the MDA content of maize embryos and endosperms increased during 5-10 min of the treatment at 100℃ and then decreased slightly. For lotus seeds: (1) activities of superoxide dismutase (SOD) and glutathione reductase (GR) of axes and cotyledons and of catalase (CAT) of axes increased during the early phase of treatment at 100℃ and then decreased; and (2) activities of ascorbate peroxidase (APX) and dehydroascorbate reductase (DHAR) of axes and cotyledons and of CAT of cotyledons gradually decreased with increasing treat-ment time at 100℃. For maize seeds: (1) activities of SOD and DHAR of embryos and endosperms and of GR of embryos increased during the early phase of the treatment at 100℃ and then decreased; and (2) activities of APX and CAT of embryos and endosperms and of GR of endosperms rapidly decreased with increasing treatment time at 100℃. With decrease in seed germination, activities of SOD, APX, CAT, GR and DHAR of axes and cotyledons of lotus seeds decreased slowly, and those of embryos and endosperms of maize seeds decreased rapidly. | DING YanFen CHENG HongYan SONG SongQuan | 2008 | Science China(Life Sciences)2008，51，9： | 4 |
| 2 | A pH-sensitive supramolecular nanosystem with chlorin e6 and triptolide co-delivery for chemo-photodynamic combination therapy显示文摘The combination of Ce6,an acknowledged photosensitizer,and TPL,a natural anticancer agent,has been demonstrated as a useful strategy to reinforce the tumor growth suppression,as well as decrease the systemic side effects compared with their monotherapy.However,in view of the optimal chemo-photodynamic combination efficiency,there is still short of the feasible nanovehicle to steadily co-deliver Ce6 and TPL,and stimuli-responsively burst release drugs in tumor site.Herein,we described the synergistic antitumor performance of a pH-sensitive supramolecular nanosystem,mediated by the host–guest complexing betweenβ-CD and acid pH-responsive amphiphilic co-polymer mPEG-PBAE-mPEG,showing the shell–core structural micelles with the tightβ-CD layer coating.Both Ce6 and TPLwere facilely co-loaded into the spherical supramolecular NPs(TPL+Ce6/NPs)by one-step nanoprecipitation method,with an ideal particle size(156.0 nm),acid pH-responsive drug release profile,and enhanced cellular internalization capacity.In view of the combination benefit of photodynamic therapy and chemotherapy,as well as co-encapsulation in the fabricated pH-sensitive supramolecular NPs,TPL+Ce6/NPs exhibited significant efficacy to suppress cellular proliferation,boost ROS level,lower MMP,and promote cellular apoptosis in vitro.Particularly,fluorescence imaging revealed that TPL+Ce6/NPs preferentially accumulated in the tumor tissue area,with higher intensity than that of free Ce6.As expected,upon 650-nm laser irradiation,TPL+Ce6/NPs exhibited a cascade of amplified synergistic chemo-photodynamic therapeutic benefits to suppress tumor progression in both hepatoma H22 tumor-bearingmice and B16 tumor-bearingmice.More importantly,lower systemic toxicitywas found in the tumor-bearingmice treated with TPL+Ce6/NPs.Overall,the designed supramolecular TPL+Ce6/NPs provided a promising alternative approach for chemo-photodynamic therapy in tumor treatment. | Yihan Wu Jingjing Li Xuemei Zhong Jinfeng Shi Yanfen Cheng Chenglin He Jiaxin Li Liang Zou Chaomei Fu Meiwan Chen Jinming Zhang Huile Gao | 2022 | Asian Journal of Pharmaceutical Sciences2022，17，2： | 4 |
| 3 | Control Efficacy of 45% Propamocarb·Denamidone SC against Potato Late Blight显示文摘The effects of different doses of 45% propamocarb·fenamidone SC on potato late blight were studied in the paper. The results showed that the overall control effect of 45% propamocarb·fenamidone SC against potato late blight on the 14 th day after the third spraying were 67. 64%- 83. 78%. The yield of potato reached 19 972. 5- 23 302. 5 kg / hm2,with higher marketable tuber rate and lower rotten tuber rate. With the increase in fungicide dose,the difference in control effect against late blight was significant,but the yield-increasing effect on potatoes was not significant. The appropriate application dosage of 45% propamocarb·fenamidone SC were 450- 675 g / hm2. | Xiao Chunfang Shen Yanfen Tian Henglin Gao Jianhua Zhang Yuanxue Chen Jiaji Cheng Qun Xu Yi | 2014 | Plant Diseases and Pests2014，5，5： | 3 |
| 4 | The community structure of Methanomassiliicoccales in the rumen of Chinese goats and its response to a highgrain diet显示文摘Background: The newly proposed methanogenic order ‘Methanomassiliicoccales' is the second largest archaeal population in the rumen, second only to the Methanobrevibacter population. However, information is limited regarding the community of this new order in the rumen.Methods: This study used real-time PCR and 454 pyrosequencing to explore the abundance and community composition of Methanomassiliicoccales in the rumen of Chinese goats fed a hay(0% grain, n = 5) or a high grain(65% grain, n = 5) diet.Results: Real-time PCR analysis showed that the relative abundance of Methanomassili coccales(% of total archaea) in the goat rumen was significantly lower in the high-grain-diet group(0.5% ± 0.2%) than that in the hay-diet group(8.2% ± 1.1%, P < 0.05). The pyrosequencing results showed that a total of 208 operational taxonomic units(OTUs) were formed from ten samples at 99% sequence identity. All the sequences were identified as Methanomassiliicoccaceae at the family level, and most of the sequences(96.82% ± 1.64%) were further classified as Group 8, 9, and 10 at the Methanomassiliicoccales genus level in each sample based on the RIM-DB database. No significant differences were observed in the number of OTUs or Chao1's, Shannon's or Pielou's evenness indexes between the hay-and high-grain-diet groups(P ≥ 0.05). PCoA analysis showed that diet altered the community of Methanomassiliicoccales.At the genus level, the relative abundances of Group 10(67.25 ± 12.76 vs. 38.13 ± 15.66, P = 0.012) and Group 4(2.07 ± 1.30 vs. 0.27 ± 0.30, P = 0.035) were significantly higher in the high-grain-diet group, while the relative abundance of Group9 was significantly higher in the hay-diet group(18.82 ± 6.20 vs. 47.14 ± 17.72, P = 0.020). At the species level, the relative abundance of Group 10 sp.(67.25 ± 12.76 vs. 38.13 ± 15.66, P = 0.012) and Group 4 sp. MpT 1(2.07 ± 1.30 vs. 0.27 ± 0.30,P = 0.035) were significantly higher in the high-grain-diet group, while the relative abundance of Group 9 sp. ISO4-G1 was significantly higher in the hay-diet group(12.83 ± 3.87 vs. 42.44 ± 18.47, P = 0.022).Conclusions: Only a few highly abundant phylogenetic groups dominated within the Methanomassiliicoccales community in the rumens of Chinese goats, and these were easily depressed by high-grain-diet feeding. The relatively low abundance suggests a small contribution on the part of Methanomassiliicoccales to the rumen methanogenesis of Chinese goats. | Wei Jin Yanfen Cheng Weiyun Zhu | 2017 | Journal of Animal Science and Biotechnology2017，8，4： | 3 |
| 5 | The effect of increased atmospheric temperature and CO_2 concentration during crop growth on the chemical composition and in vitro rumen fermentation characteristics of wheat straw显示文摘This experiment was c onducted to investigate the effects of increased atmospheric temperature and CO_2concentration during crop growth on the chemical composition and in vitro rumen fermentation characteristics of wheat straw.The field experiment was carried out from November 2012 to June 2013 at Changshu(31°32'93 'N,120°41'88' E) agro-ecological experimental station.A total of three treatments were set.The concentration of CO_2was increased to 500 umol/mol in the first treatment(CO_2 group).The temperature was increased by 2 ℃ in the second treatment(TEM group) and the concentration of CO_2 and temperature were both increased in the third treatment(CO_2 + TEM group).The mean temperature and concentration of CO_2 in control group were 10.5 ℃ and413 umol/mol.At harvesting,the wheat straws were collected and analyzed for chemical composition and in vitro digestibility.Results showed that dry matter was significantly increased in all three treatments.Ether extracts and neutral detergent fiber were significantly increased in TEM and CO_2 + TEM groups.Crude protein was significantly decreased in CO_2 + TEM group.In vitro digestibility analysis of wheat straw revealed that gas production was significantly decreased in CO_2 and CO_2 + TEM groups.Methane production was significantly decreased in TEM and CO_2 + TEM groups.Ammonia nitrogen and microbial crude protein were significantly decreased in all three treatments.Total volatile fatty acids were significantly decreased in CO_2 and CO_2 + TEM groups.In conclusion,the chemical composition of the wheat straw was affected by temperature and CO_2 and the in vitro digestibility of wheat straw was reduced,especially in the combined treatment of temperature and CO_2. | Xiangyu He Yanping Wu Min Cai Chunlong Mu Weihong Luo Yanfen Cheng Weiyun Zhu | 2015 | Journal of Animal Science and Biotechnology2015，6，4： | 1 |
| 6 | Control Effect of UAV Low Volume Spray Technology on Thrips and Tomato Spotted Wilt Virus in Tobacco Field显示文摘[Objectives]The paper was to explore the influence of unmanned aerial vehicle(UAV)low volume spray technology on the control effect of viruliferous thrips and tomato spotted wilt virus(TSWV)under different pesticide concentrations,and to explore the effective control nodes and methods of thrips and TSWV.[Methods]According to the occurrence characteristics of thrips and TSWV,the field control effects of 4 pesticides on thrips and TSWV were tested by UAV with the thrips number and disease index as indicators,following the principle of regional and periodic control trials.[Results]In the groups of UAV and artificial prevention and control,4 agents were used alternatively,including 1%emamectin benzoate ME,1.7%abamectin imidacloprid ME,5%emamectin benzoate WDG,and 30%pyriproxyfen dinotefuran SL.There was no significant difference in the control effect of thrips and TSWV among treatment 1(recommended dosage of UAV agent),treatment 2(reduction of recommended dosage of UAV agent by 25%)and treatment 4(recommended dosage of artificial control agent),and the control effects on thrips were more than 83.16%in the 3 treatments.The disease index of TSWV in the 3 treatments decreased from 8.64±1.37 in the blank control group to less than 3,which reached the prevention and control threshold.But treatment 3(reduction of recommended dosage of UAV agent by 50%)did not reach the prevention and control threshold.The disease index of TSWV in the blank control area increased with the increase of the number of live nymphs of thrips,and there was a significant positive correlation,with good fitness.[Conclusions]UAV has a significant impact on the control effect of thrips and TSWV under different pesticide concentrations.In the actual flight control application,it is recommended that the amount of the pesticides is about 3/4 and not less than 1/2 of that of the artificial application.The control area should be extended to 100 m of the ridge of the tobacco field,and UAV is used periodically to control thrips and TSWV for 5 times from pre-transplanting stage to pre-squaring stage of flue-cured tobacco.The control effect is significantly different from the traditional artificial application. | Zefan LIU Dean XIE Jiabao HAN Yanfen XIE Li MA Siyuan GAO Ruiqi PENG Yuting ZHAO Xiangze FU Wengang CHENG Yonghong FENG Yan ZHANG Huaping ZHANG Ying HUANG Leijie DAI Lanfeng WANG | 2023 | Plant Diseases and Pests2023，14，4： | 0 |
| 7 | The uniqueness and superiority of energy utilization in yaks compared with cattle in the highlands:A review显示文摘Yaks living on the Qinghai-Tibetan Plateau for a long time have evolved a series of mechanisms to adapt to the unique geographical environment and climate characteristics of the plateau.Compared with other ruminants,yaks have higher energy utilization and metabolic efficiency.This paper presents possible mechanisms responsible for the efficient energy utilization,absorption and metabolism resulting from the unique evolutionary process of yaks.It is hoped that the information discussed in this review will give a better insight into the uniqueness and superiority of yaks in regards to energy metabolism and utilization compared with cattle and open new avenues for the targeted regulation of energy utilization pathways of other ruminants. | Xiaojing Liu Jian Gao Suozhu Liu Yanfen Cheng Lizhuang Hao Shujie Liu Weiyun Zhu | 2023 | Animal Nutrition2023，，1： | 0 |
| 8 | Characterization of a novel bifunctional enzyme from buffalo rumen metagenome and its effect on in vitro ruminal fermentation and microbial community composition显示文摘To efficiently use lignocellulosic materials in ruminants,it is crucial to explore effective enzymes,especially bifunctional enzymes.In this study,a novel stable bifunctional cellulase-xylanase protein from buffalo rumen metagenome was expressed and characterized,CelXyn2.The enzyme displayed optimal activity at pH 6.0 and 45℃.The residual endoglucanase and xylanase activities were 90.6%and 86.4%after a 60-min pre-incubation at 55℃.Hydrolysis of rice straw,wheat straw,sheepgrass and sugar beet pulp by CelXyn2 showed its ability to degrade both cellulose and hemicellulose polymers.Treatment with CelXyn2 improved the hydrolysis of agricultural residues with an evident increase in production of total gas,lactate and volatile fatty acids.The results of 16S rRNA and real-time PCR showed that the effect on in vitro ruminal microbial community depended on fermenta-tion substrates.This study demonstrated that CelXyn2 could strengthen lignocellulose hydrolysis and in vitro ruminal fermentation.These characteristics of CelXyn2 distinguish it as a promising candidate for agricultural application. | Zhenxiang Meng Jing Ma Zhanying Sun Chengjian Yang Jing Leng Weiyun Zhu Yanfen Cheng | 2023 | Animal Nutrition2023，，2： | 0 |
| 9 | Production,purific tion,characterization and application of two novel endoglucanases from buffalo rumen metagenome显示文摘Background Lignocellulose biomass is the most abundant and renewable material in nature.The objectives of this study were to characterize two endoglucanases Trep Cel3 and Trep Cel4,and determine the effect of the combination of them(1.2 mg Trep Cel3,0.8 mg Trep Cel4)on in vitro rumen fermentation characteristics.In this study,three nature lignocellulosic substrates(rice straw,RS;wheat straw,WS;leymus chinensis,LC)were evaluated for their in vitro digestibility,gas,NH3-N and volatile fatty acid(VFA)production,and microbial protein(MCP)synthesis by adding enzymatic combination.Methods Two endoglucanases’genes were successfully expressed in Escherichia coli(E.coli)BL21(DE3),and enzymatic characteristics were further characterized.The combination of Trep Cel3 and Trep Cel4 was incubated with lignocellulosic substrates to evaluate its hydrolysis ability.Results The maximum enzymatic activity of Trep Cel3 was determined at p H 5.0 and 40℃,while Trep Cel4 was at p H 6.0 and 50℃.They were stable over the temperature range of 30 to 60℃,and active within the p H range of 4.0 to 9.0.The Trep Cel3 and Trep Cel4 had the highest activity in lichenan 436.9±8.30 and 377.6±6.80 U/mg,respectively.The combination of Trep Cel3 and Trep Cel4 exhibited the highest efficiency at the ratio of 60:40.Compared to maximum hydrolysis of Trep Cel3 or Trep Cel4 separately,this combination was shown to have a superior ability to maximize the saccharification yield from lignocellulosic substrates up to 188.4%for RS,236.7%for wheat straw WS,222.4%for LC and 131.1%for sugar beet pulp(SBP).Supplemental this combination enhanced the dry matter digestion(DMD),gas,NH3-N and VFA production,and MCP synthesis during in vitro rumen fermentation.Conclusions The Trep Cel3 and Trep Cel4 exhibited the synergistic relationship(60:40)and significantly increased the saccharification yield of lignocellulosic substrates.The combination of them stimulated in vitro rumen fermentation of lignocellulosic substrates.This combination has the potential to be a feed additive to improve agricultural residues utilization in ruminants.If possible,in the future,experiments in vivo should be carried out to fully evaluate its effect. | Zhenxiang Meng Chengjian Yang Jing Leng Weiyun Zhu Yanfen Cheng | 2023 | Journal of Animal Science and Biotechnology2023，14，3： | 0 |
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