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| 1 | Reconstruction of chemically burned rat corneal surface by bone marrow-derived human mesenchymal stem cells显示文摘 | Ma Y Xu Y Xiao Z Yang W Zhang C Song E Du Y Li L | 2006 | 中国生物学文摘2006，20，5： | 58 |
| 2 | Multiple genetic alterations and behavior of cellular biology in gastric cancer and other gastric mucosal lesions:H.pylori infection,histological types and staging显示文摘AIM To investigate the expression of multiplegenes and the behavior of cellular biology ingastric cancer(GC)and other gastric mucosallesions and their relations to Helicobacter pylori(H.pylori)infection,tumor staging andhistological subtypes.METHODS Three hundred and twenty-sevenspecimens of gastric mucosa obtained viaendoscopy or surgical resection,and ABCimmunohistochemical staining were used todetect the expression of p53,p16,Bcl-2 andCOX-2 proteins.H.pylori was determined byrapid urea test combined with pathologicalstaining or14C urea breath test.Cellular image analysis was performed in 66 patients withintestinal metaplasia(IM)and/or dysplasia(Dys).In 30 of them,both cancer and theparacancerous tissues were obtained at the timeof surgery.Histological pattern,tumor staging,lymph node metastasis,grading ofdifferentiation and other clinical data werestudied in the medical records.RESULTS p16 expression of IM or Dys wassignificantly lower in positive H.pylori chronicatrophic gastritis(CAG)than those withnegative H.pylori(CAG:54.8% vs 88.0%,IM:34.4% vs 69.6%,Dys:23.8% vs 53.6%,allP<0.05),Bcl-2 or COX-2 expression of IM orDys in positive H.pylori cases was significantlyhigher than that without H.pylori(Bcl-2:68.8%vs23.9%,90.5% vs 60.7%;COX-2:50.0% vs10.8%,61.8% vs 17.8%;all P<0.05).Themean number of most parameters of cellularimage analysis in positive H.pylori group wassignificantly higher than that in negative H.pylori group(Ellipser:53±14,40±12μm,Area1:748±572,302±202 μm2,Area2:3050±1661,1681±1990 μm2,all P<0.05;Ellipseb:79±23,58±15 μm,Ratio1:22%±5%,13%±4%,Ratio2:79%±17%,53%±20%,all P<0.01).There was significant correlation between Bcl-2and histologic pattern of gastric carcinoma,andbetween COX-2 and tumor staging or lymph nodemetastasis(Bcl-2:75.0% vs 16.7%;COX-2:76.0% vs 20.0%,79.2% vs 16.7%;allP<0.05).CONCLUSION p1l6, Bcl-2, and COX-2 but not p53 gene may play a role in the early genesis/ progression of gastric carcinoma and are associated with H. pylori infection. p53 gene is relatively late event in gastric tumorigenesis and mainly relates to its progression. There is more cellular-biological behavior of malignant tumor in gastric mucosal lesions with H. pylori infection. Aberrant Bcl-2 protein expression appears to be preferentially associated with the intestinal type cancer. COX-2 seems to be related to tumor staging and lymph node metastasis. | Heng Jun Gao Lian Zhen Yu Jian Feng Bai Yan Shen Peng Gu Sun Han Lin Zhao Kun Miu Xiu Zhen Lü Xiao Yong Zhang Zhi Quan Zhao | 2000 | World Journal of Gastroenterology2000，6，6： | 52 |
| 3 | Vasculogenic mimicry is a prognostic factor for postoperative survival in patients with glioblastoma显示文摘A previous report has confirmed the existence and clinical significance of vasculogenic mimicry(VM)in glioma.However,its conclusions about the negative clinical significance of VM in glioblastoma are based on a small group of patients and,thus,might be unconvincing.The aim of the present study was to reevaluate the clinical significance of VM in glioblastoma.Patients were classified as VM-positive or | Wang SY Ke YQ Lu GH Song ZH Yu L Xiao S Sun XL Jiang XD Yang ZL Hu CC | 2013 | 中国神经肿瘤杂志2013，11，3： | 22 |
| 4 | Down-regulation of Hsp90 could change cell cycle distribution and increase drug sensitivity of tumor cells显示文摘:AIM To construct Hsp90 antisense RNAeukaryotic expression vector, transfect it intoSGC7901 and SGC7901/VCR of MDR-type humangastric cancer cell lines, HCC7402 of humanhepatic cancer and Eel09 of human esophagealcancer cell lines, and to study the cell cycledistribution of the gene transected cells andtheir response to chemotherapeutic drugs.METHODS A I .03kb cDNA sequence of Hsp90Pwas obtained from the primary plasmid phHsp90by EcoR 1 and BamH I nuclease digestion andwas cloned to the EcoR 1 and BamH 1 site ofthe pcDNA by T4DNA ligase and an antisenseorientation of Hsp900 expression vector wasconstructed. The constructs were transfectedwith lipofectamine and positive clones wereselected with G418. The expression of RNA wasdetermined with dot blotting and RNaseprotection assay, and the expression of Hsp90protein determined with Western blot. Cell cycledistribution of the transfectants was analyzedwith flow cytometry, and the drug sensitivity ofthe transfectants to adriamycin (ADR ),vincrinstine (VCR ), mitomycin (MMC ) andcyclophosphamide (CTX ) with MTT andintracellular drug concentration of thetransfectants was determined with flowcytometry.RESULTS In EcoR 1 and BamH I restrictionanalysis, the size and the direction of the clonedsequence of Hsp900 remained what had beendesigned and the gene constructs were namedpcDNA-Hsp90. AH^SGC7901, AH^SGC7901/ VCR,AH-HCC7402 and AH-Eel09 cell clones allexpressed Hsp90 anti--sense RNA. Theexpression of Hsp90 was down--regulated in AHSGC7901, AH--SGC7901/ VCR, AH-HCC7402 andAH--Eel09 cell clones. Cell cycle distribution waschanged differently. In AH-SGC7901/ VCR andAH-Ec109 cells, G, phase cells were increased; Sphase and G, phase cells were decreased ascompared with their parental cell lines. In AHSGC7901 cell, G, phase cells were decreased, Qphase cells increased and S phase cells were notchanged, and in AH-HCC7402 cells G,, S and qphase cells remained unchanged as comparedwith their parental cell lines. The sensitivity ofAH--SGC7901, AH--SGC7901/ VCR, AH-HCC7402 andAH-Ec109 to chemotherapeutic drugs, thesensitivity ot AH--SGC7901/ VCR to ADR, VCR,MMC and CTX the sensitivity of AH-HCC7402 toADR and VCR, and the sensitivity of Eel09 toADR, VCR and CTX all increased as comparedwith their parental cell lines. The meanfluorescence intensity of ADR in AH--SGC7901,AH-SGC7901/ VCR, AH--HCC7402 and AH-Ec109was also significantly elevated (P< 0. 05).CONCLUSION Down-regulation of HsP90 couldchange cell cycle distribution and increase thedrug sensitivity of tumor cells. | Liu XL Xiao B Yu ZC Guo JC Zhao QC Xu L Shi YQ Fan DM | 1999 | World Journal of Gastroenterology1999，5，3： | 21 |
| 5 | Spatio-temporal variation of vegetation in an arid and vulnerable coal mining region显示文摘Environmental assessment in an arid coal mining area requires an understanding of the influences of coal mining,the arid climate and ecological remediation.To that end,we selected vegetation as the key environmental factor to observe.Remote sensing approaches to monitoring the spatio-temporal variation of vegetation caused by mining activities,the arid climate and ecological remediation in the Shengdong coal mining area are described.Over a large regional scale it was found that the vegetation was improved as a result of ecological remediation activities.At the local scale,however,the vegetation coverage and soil moisture in the mined areas were slightly lower than those in un-mined areas due to mining subsidence.These differences are partly attributed to ground fissures that injure root systems and increase the depletion of soil moisture.It is recommended that fissures be reduced and filled to lessen their adverse effects on the environment. | LEI Shaogang BIAN Zhengfu DANIELS John L HE Xiao | 2010 | Mining Science and Technology2010，20，3： | 20 |
| 6 | 门静脉高压并非肝细胞癌肝切除术的绝对禁忌证显示文摘在肝细胞癌(hepatocellular carcinoma,HCC)的各种临床分期系统中,Bruix J团队领衔的巴塞罗那肝癌临床分期标准(Barcelona Clinic Liver Cancer,BCLC)最受推崇,美国和欧洲肝病协会指南的制定亦基于该分期系统。BCLC认为门静脉高压显著增加HCC肝切除术后患者发生肝功能衰竭的风险, | Zhong JH Li H Xiao N Ye XP Ke Y Wang YY Ma L Chen J You XM Zhang ZY Lu SD Li LQ 钟鉴宏 齐鲁楠 | 2014 | 中国癌症防治杂志2014，6，4： | 18 |
| 7 | Combined acoustic radiation force impulse, aminotransferase to platelet ratio index and Forns index assessment for hepatic fibrosis grading in hepatitis B显示文摘AIM: To investigate the combined diagnostic accuracy of acoustic radiation force impulse(ARFI), aspartate aminotransferase to platelet ratio index(APRI) and Forns index for a non-invasive assessment of liver fibrosis in patients with chronic hepatitis B(CHB). METHODS: In this prospective study, 206 patients had CHB with liver fibrosis stages F0-F4 classified by METAVIR and 40 were healthy volunteers were measured by ARFI, APRI and Forns index separately or combined as indicated. RESULTS: ARFI, APRI or Forns index demonstrated a significant correlation with the histological stage(all P < 0.001). According to the AUROC of ARFI and APRI for evaluating fibrotic stages more than F2, ARFI showed an enhanced diagnostic accuracy than APRI(P < 0.05). The combined measurement of ARFI and APRI exhibited better accuracy than ARFI alone when evaluating ≥ F2 fibrotic stage(Z = 2.77, P = 0.006). Combination of ARFI, APRI and Forns index did not obviously improve the diagnostic accuracy compared to the combination of ARFI and APRI(Z = 0.958, P = 0.338). CONCLUSION: ARFI + APRI showed enhanced diagnostic accuracy than ARFI or APRI alone for significant liver fibrosis and ARFI + APRI + Forns index shows the same effect with ARFI + APRI. | Chang-Feng Dong Jia Xiao Ling-Bo Shan Han-Ying Li Yong-Jia Xiong Gui-Lin Yang Jing Liu Si-Min Yao Sha-Xi Li Xiao-Hua Le Jing Yuan Bo-Ping Zhou George L Tipoe Ying-Xia Liu | 2016 | World Journal of Hepatology2016，8，14： | 18 |
| 8 | Pollen indication to source plants in the eastern desert of China显示文摘In order to investigate the indication of major pollen types to source plants, 39 surface pollen samples are collected from 39 plant communities. The results show that diversity of pollen assemblages presents in different commu-nities. Chenopodiaceae pollen percentages are highest in Kalidium communities and Ceratoides communities (higher than 45%, average is 63.4%), but high Ephedra pollen per-centages (higher than 3.3%, average is 16.5%)in Ceratoides communities make it distinguished from Kalidium communi-ties. Artemisia pollen percentages (more than 50%) are much higher than Chenopodiaceae (less than 35%) in Artemisia Communities. Nitraria pollen percentages (higher than 2%, average is 13.6%) are highest in Nitraria communities com-pared with those in other community patterns. Tamariceae pollen percentages (higher than 2%, average is 8.6%) are highest both in Tetraena and Reaumuria communities com-pared with those in other community patterns. But in Reau-muria communities, Artemisia pollen percentages are higher than Chenopodiaceae with some other pollen types such as Gramineae, Compositae, Leguminosae; on the contrary, in Tetraena communities, Artemisia pollen percentages are less than Chenopodiaceae with few other pollen types. Calli-gonum pollen only appears in Calligonum communities (higher than 2%,average is 5%). The results of cluster analysis (CLA) and detrended correspondence analysis (DCA) show that the pollen indication to source plants can be reflected by association indices (A), over-representation in-dices (O), under-representation indices (U), R-value, the av-erage pollen percentages where parent plants are absent (Xa) and the average pollen percentages where parent plants are present (Xp). Zygophyllum, Calligonum, Tetraena, Tribulus and Tamariceae can indicate source plants very well, owing to high A scores. Artemisia, Chenopodiaceae and Gramineae have high R-values, and Nitraria, Cruciferae and sedum have high O scores, so they are over-represented types, but the higher Xp and the lower Xa values make that these pollen types can also indicate source plants moderately. Allium, Leguminosae, Roseceae, Pedicularis and Compositae have low A scores (less than 0.5), high O and/or U scores, so they cannot indicate source plants clearly. Some other pollen types, such as Pinus, Picea, Betula, Quercus, Ulmus, Ephedra, Elaegnaceae, Ranunculaceae, Caryophyllaceae, Polygona-ceae, Saxifragaceae, only appear in pollen assemblages, so their indication cannot be decided. Just like the pollen per-centage ratios of Artemisia and Chenopodiaceae (A/C), the ratios of Ephedra and Artemisia (E/A), Nitraria and Ephedra (N/E) can also indicate the aridity of environment. In typical desert communities, A/C ratios are less than 0.5, E/A ratios are more than 0.1, and N/E ratios are less than 1. In steppe desert, A/C ratios are 0.5―2, E/A ratios are less than 0.1, and N/E ratios are more than 1. | LI Yuecong XU Qinghai ZHAO Yinclkui YANG Xiaolan XIAO Jule CHEN Hui Lü Xinmiao | 2005 | Chinese Science Bulletin2005，50，15： | 17 |
| 9 | Evolution of vegetation and climate since the last glacial maximum recorded at Dahu peat site, South China显示文摘Sporopollen analysis on a 346 cm peat record at Dahu, Jiangxi, chronologically constrained by 16 AMS 14C datings, provides an opportunity to reconstruct the vegetation evolution stages responding to cli-mate change in South China since the last glacial maximum. The result shows that during 18330-15630 cal a B.P., broad-leaved forest dominated the area, corresponding to mild, cool and fairly humid climate. At the interval of 15630-11600 cal a B.P., several evergreen broad-leaved species appeared within the broad-leaved forest, indicating moderate and humid condition. During early Holocene, broad-leaved evergreen forest community was constructed as Castanopsis/Lithorcarpus principally developed, suggesting a warm and humid scenario until 6000 cal a B. P. Since 6000 cal a B. P., abrupt forest deterioration happened with an contemporary increase of fern and herb communities, repre-senting a turnover to relatively cool and dry condition and as well, possible impact from human activi-ties. Meanwhile, several relatively cool and dry events can be identified in the sporopollen record, they can be correlated to the North Atlantic Heinrich event, YD and Holocene millennial-scale oscillations, implying that the low latitude climate was coupled with high latitude influences. Moreover, the varia-tions of temperature and humidity since LGM at Dahu were much smaller when compared with the re-cords in north monsoonal China. | XIAO JiaYi Lü HaiBo ZHOU WeiJian ZHAO ZhiJun HAO RuiHui | 2007 | Science China Earth Sciences2007，50，8： | 15 |
| 10 | 1990—2017年中国伤害负担:2017年全球疾病负担研究结果(摘译)显示文摘伤害是全球性的公共卫生挑战,涉及各年龄组和不同性别的人群。过去三十年来,中国经济快速发展,人口老龄化趋势加重,社会呈现出城镇化、机动化和老龄化等特征。随着人们生活环境和生活方式的改变,伤害已跃居人群致死原因第五位,仅排在恶性肿瘤、心脑血管疾病、呼吸系统疾病和心肌梗死之后。据估计,中国每年在伤害方面消耗的直接医疗费用约为650亿元人民币. | 林泽婷(译) 吕来文(译) 黄晓晴(译) 李丽萍(审校) Duan Leilei Ye Pengpeng Juanita A Haagsma Jin Ye Wang Yuan Er Yuliang Deng Xiao Gao Xin Ji Cuirong Wang Linhong Marlena S Bannick W Cli Mountjoy-Venning Caitlin N Hawley Zichen Liu Mari Smith Spencer L James Theo Vos Christopher J L Murray | 2020 | 伤害医学(电子版)2020，9，2： | 15 |
| 11 | Therapeutic approaches to non-alcoholic fatty liver disease: past achievements and future challenges显示文摘BACKGROUND: Non-alcoholic fatty liver disease (NAFLD) is a leading cause of chronic liver injury and mortality in Western countries and China. However, as to date, there is no direct and effective therapy for this disease. The aim of this review is to analyze the key progress and challenges of main current therapeutic approaches in NAFLD. DATA SOURCE: We carried out a PubMed search of English-language articles relevant to NAFLD therapy. RESULTS: There are two major therapeutic strategies for NAFLD treatment: (1) lifestyle interventions (including weight reduction, dietary modification and physical exercise) and (2) pharmaceutical therapies. Lifestyle interventions, particularly chronic and moderate intensity exercise, are the most effective and recognized clinical therapies for NAFLD. For pharmaceutical therapies, although their effects and mechanisms have been extensively investigated in laboratory studies, they still need further tests and investigations in clinical human trials. CONCLUSION: Future advancement of NAFLD therapy should focus on the mechanistic studies on cell based and animal models and human clinical trials of exercise, as well as the combination of lifestyle intervention and pharmaceutical therapy specifically targeting main signaling pathways related to lipid metabolism, oxidative stress and inflammation. | Jia Xiao Rui Guo Man Lung Fung Emily C Liong George L Tipoe | 2013 | Hepatobiliary & Pancreatic Diseases International2013，12，2： | 14 |
| 12 | Probabilistic assessment of tunnel convergence considering spatial variability in rock mass properties using interpolated autocorrelation and response surface method显示文摘This study aims at the probabilistic assessment of tunnel convergence considering the spatial variability in rock mass properties. The method of interpolated autocorrelation combined with finite difference analysis is adopted to model the spatial variability of rock mass properties. An iterative procedure using the first-order reliability method(FORM) and response surface method(RSM) is employed to compute the reliability index and its corresponding design point. The results indicate that the spatial variability considerably affects the computed reliability index. The probability of failure could be noticeably overestimated in the case where the spatial variability is neglected. The vertical scale of fluctuation has a much higher effect on the probabilistic result with respect to the tunnel convergence than the horizontal scale of fluctuation. And the influence of different spacing of control points on the computational accuracy is investigated. | Qing Lü Zhipeng Xiao Jun Zheng Yuequan Shang | 2018 | Geoscience Frontiers2018，9，6： | 12 |
| 13 | Effect of high-molecular-weight glutenin subunit deletion on soft wheat quality properties and sugar-snap cookie quality estimated through near-isogenic lines显示文摘High-molecular-weight glutenin subunits(HMW-GSs) play a critical role in determining the viscoelastic properties of wheat dough. The HMW-GSs are encoded by Glu-A1, Glu-B1, and Glu-D1 loci on the long arms of chromosomes 1A, 1B, and 1D, respectively. In the present study, four near-isogenic lines with different HMW-GS deletions and compositions at the Glu-A1 and Glu-D1 loci in Yangmai 18 background were used for quality analysis. Deletion in Glu-D1 showed much weaker gluten quality and dough strength than null Glu-A1 genotype and wild genotype(WT), based on the measurements of sodium dodecyl sulfate(SDS)-sedimentation, lactic acid solvent retention capacity(SRC), gluten index, development time, stability time, and alveograph P and L values. The deletion of Glu-D1 did not significantly affect grain hardness, grain protein content, water SRC, sodium carbonate SRC, and sucrose SRC. Double null genotype in Glu-A1 and Glu-D1 and single null genotype in Glu-D1 showed significantly higher cookie diameter, crispness, and lower cookie height compared with single null genotype in Glu-A1 and WT. These indicate that the null Glu-D1 genotype is useful for improvement of biscuit quality, and use of this germplasm would be a viable strategy to develop new wheat varieties for biscuit processing. | ZHANG Xiao ZHANG Bo-qiao WU Hong-ya LU Cheng-bin Lü Guo-feng LIU Da-tong LI Man JIANG Wei SONG Gui-hua GAO De-rong | 2018 | Journal of Integrative Agriculture2018，17，5： | 11 |
| 14 | Microarray-based analysis of changes in diversity of microbial genes involved in organic carbon decomposition following land use/cover changes显示文摘 | Zhang YG Zhang XQ Liu XD Xiao Y Qu L J Wu LY Zhou JZ | 2007 | 中国生物学文摘2007，21，8： | 10 |
| 15 | Development of genome-wide insertion/deletion markers in rice based on graphic pipeline platform显示文摘DNA markers play important roles in plant breeding and genetics.The Insertion/Deletion(InDel) marker is one kind of co-dominant DNA markers widely used due to its low cost and high precision.However,the canonical way of searching for InDel markers is time-consuming and laborintensive.We developed an end-to-end computational solution(InDel Markers Development Platform,IMDP) to identify genome-wide InDel markers under a graphic pipeline environment.IMDP constitutes assembled genome sequences alignment pipeline(AGA-pipe) and next-generation resequencing data mapping pipeline(NGS-pipe).With AGA-pipe we are able to identify 12,944 markers between the genome of rice cultivars Nipponbare and 93-11.Using NGS-pipe,we reported 34,794 InDels from re-sequencing data of rice cultivars Wu-Yun-Geng7 and Guang-Lu-Ai4.Combining AGApipe and NGS-pipe,we developed 205,659 InDels in eight japonica and nine indica cultivars and 2,681 InDels showed a subgroup-specific pattern.Polymerase chain reaction(PCR)analysis of subgroup-specific markers indicated that the precision reached 90%(86 of 95).Finally,to make them available to the public,we have integrated the InDels/markers information into a website(Rice InDel Marker Database,RIMD,http://gffzz71b3756633b74a6ehb0cvcqpkwk9f6on0.ffgz.tsg.suse.edu.cn/).The application of IMDP in rice will facilitate efficiency for development of genome-wide InDel markers,in addition it can be used in other species with reference genome sequences and NGS data. | Yang L Xiao Cui Rui Li Piaopiao Huang Jie Zong Danqing Yao Gang Li Dabing Zhang Zheng Yuan | 2015 | Journal of Integrative Plant Biology2015，57，11： | 9 |
| 16 | A Rapid Analysis Tool for Aerodynamics/Aerothermodynamics of Hypersonic Vehicles显示文摘A rapid engineering surface panel method to analyze aerodynamics and aerothermodynamics of hypersonic vehicles is developed.To obtain the surface pressure distribution of a hypersonic vehicle,the local surface inclination method is applied to calculate the pressure coefficient for each surface panel element,of which the normal vector is corrected first by using an efficient data structure and Rey-casting algorithm,local Reynolds numbers are calculated according to the geometric streamline method,then the aerodynamic heating flux is computed by both reference enthalpy relations and Reynolds analogy method.Several typical test cases are performed and the results indicate that,the developed tool is effective in predicting the aerodynamics/aerothermodynamics for complex geometry of hypersonic vehicle in a wide range of Mach numbers with a sufficient accuracy. | Li Zhengzhou Xiao Tianhang Lü Fanxi Yu Xiongqing Zhang Zhenming Du Sha | 2017 | Transactions of Nanjing University of Aeronautics and Astronautics2017，34，4： | 7 |
| 17 | Effects of cooling rates on the mechanical properties of a Ti-based bulk metallic glass显示文摘Mechanical properties of the glassy specimens fabricated at different cooling rates with a composition of Ti40Zr25Cu12Ni3Be20 were systematically investigated. It was confirmed that faster cooling rates caused not only a larger amount of frozen-in free volume but also a higher glass transition temperature in the bulk glassy alloy. Increase in the free volume was found to favor plastic deformation and then to give rise to larger compressive plasticity, whilst the rise in the glass transition temperature seemed to be closely related to the higher yield strength. Moreover, the increase of yield strength and plasticity induced by fast cooling rates may also be associated with the residual stress generated during the fabrication process. Our results suggest that the deformation behavior of bulk metallic glasses is sensitive to various factors and influences from the other factors should be excluded as far as cooling-rate effects on bulk metallic glasses are considered. | XIAO YueHua, WU Yuan, LIU ZhiYuan, WU HongHui & Lü ZhaoPing State Key Laboratory for Advanced Metals and Materials, University of Science and Technology Beijing, Beijing 100083, China | 2010 | Science China(Physics,Mechanics & Astronomy)2010，53，3： | 6 |
| 18 | Eukaryotic DNA damage tolerance and translesion synthesis through covalent modifications of PCNA显示文摘除了明确的脱氧核糖核酸修理小径,所有生活有机体发展了机制避免复制叉倒塌在复制由于脱氧核糖核酸的造成分裂的共有原子价修正被堵住的一个地点引起的细胞死亡。术语脱氧核糖核酸损坏忍耐(DDT ) 泛泛地被采用了包括房间由熬过堵住复制的损害与或没有联系 genomic 不稳定性的许多机制。最近的基因分析在优核质显示那 DDT,从酵母到人,与是无差错的和另外一个由二条平行小径组成高度诱变。在开始发育的酵母,有趣地,这二条小径被增殖的房间的顺序的修正调停由二 ubiquitination 建筑群 Rad6-Rad18 和 Mms2-Ubc13-Rad5 的原子抗原(PCNA ) 。由 Rad6-Rad18 的 PCNA 的导致损坏的 monoubiquitination 与增加的傻瓜发育不全支持 translesion 合成(TLS ) ,当在由 Mms2-Ubc13-Rad5 的一样的 K164 残余的 PCNA 的随后的 polyubiquitination 支持无差错的损害时绕过。从最近的研究获得的数据建议上述机制在更高的优核质被保存。特别地,哺乳动物包含多重专业化 TLS 聚合酶。在 TLS 聚合酶之一的缺点被连接了到 genomic 不稳定性和癌症。 | Parker L Andersen Fang Xu Wei Xiao | 2008 | Cell Research2008，18，1： | 6 |
| 19 | Interleukin-1α, 6 regulate the secretion of vascular endothelial growth factor A, C in pancreatic cancer显示文摘Vascular endothelial growth factor (VEGF, namely VEGF-A) is an angiogenic polypeptide and VEGF-C is a lymphangiogenic polypeptide that has been implicated in cancer growth, invasion and metastasis. Several cytokines and growth factors play an important part in cancer progression. These cytokines and growth factors are the principal mediators of cancer cells-stromal cell interaction , which is critical for invasion of cancer cells to the surrounding tissues and metastatic dissemination to distant organs. In this study, we studied VEGF-A, C expression in cultured human pancreatic cancer cell lines and whether the presence of VEGF-A, C in the cell lines is regulated by cytokines interleukin-lct (EL-1α), and interleukin-6 (IL-6). METHODS: We used Northern blot and Western blot methods to analyze expression of the gene and protein of VEGF-A, C in all 6 tested cell lines (ASPC-1, CAPAN-1, MIA-PaCa-2, PANC-1, COLO-357 and T3M4) respectively. To analyze what is the regulator for this VEGF-A, C expression in pancreatic cancer,we used the reverse transcription -polymerase chain reaction (RT-PCR) method to analyze VEGF-A, C expression in cultured human pancreatic cancer cell lines (CAPAN-1 and COLO-357) under the stimulation with IL-1α (10μg/L) or IL-6 (100 μg/L). RESULTS:Northern blot analysis revealed the presence of the 4.1-kb VEGF-A mRNA transcript and 2.4-kb VEGF-C mRNA transcript in all 6 tested cell lines. Immunoblotting with highly specific anti-VEGF-A, anti-VEGF-C antibody revealed the presence of a molecular weight of 43-kDa VEGF-A protein and 55-kDa VEGF-C protein in all the cell lines. RT-PCR analysis revealed the levels of the VEGF-A and VEGF-C gene were 1-2 fold and a 1-fold increase in the COLO-357 cell line by stimulation with IL-la, however, no effect was found in the CAPAN-1 cell line. The levels of the VEGF-A and VEGF-C gene were 2-5 fold and a 1-fold increase in the CAPAN-1 cell line by stimulation with IL-6, but, no effect was found in the COLO-357 cell line. CONCLUSION:These findings suggested that the expression of VEGF-A, C and their regulation by IL-1α, IL-6 in pancreatic cancer contributes to the lymphatic and distant metastasis and the disease progression. | Department of Hepatobiliary Surgery (Tang RF, Zhang FR, Peng L, Wang SX, Xiao Y and Zhang M) and Department of Dermatology (Wang SX), 4th Hospital, Hebei Medical University, Shijiazhuang 050011, China | 2005 | Hepatobiliary & Pancreatic Diseases International2005，4，3： | 6 |
| 20 | Induction of mucosal and systemic immune response by single-dose oral immunization with biodegradable microparticles containing DNA encoding HBsAg显示文摘 | He XW Wang F Jiang L Li J Liu SK Xiao ZY Jin XQ Zhang YN He Y Li K Guo YJ Sun SH | 2005 | 第二军医大学学报2005，26，10： | 5 |
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