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| 1 | Plasma membrane proteome analysis of the early effect of alcohol on liver: implications for alcoholic liver disease显示文摘在人,酒精的在消费上能导致严肃的肝疾病。在肝疾病上检验酒精的早效果,老鼠被给足够的乙醇开发肝肝硬化。在纤维变性的发作前的老鼠在这个工作被学习。肝的血浆膜(下午) 被提取由两次蔗糖密度坡度 centrifugation。从对待乙醇的老鼠和控制的下午的 proteome 侧面为相对、绝对的 quantitation (iTRAQ ) 用二维的胶化电气泳动(2-DE ) 和等压的标签被分析技术。乙醇处理改变了 15 不同的肝蛋白质的数量:他们中的 10 个被 2-DE 检测并且 5 由 iTRAQ。角质素 8 被两个方法检测。这些差别的基因本体论分析检测了显示的蛋白质他们的那个大多数涉及象有约束力的活动那样的重要房间函数(包括离子, DNA, ATP 绑定,等等) ,房间结构,或酶活动。在这些之中, annexin A2,角质素 8,和角质素 18 进一步用西方的污点分析被验证, annexin A2 被 immunohistochemistry 验证。我们的结果建议酒精有潜力由在 PM 改变几相关蛋白质的表示层次影响房间结构,粘附和酶活动。就我们的知识而言,这是第一次在肝下午 proteome 上学习酒精的效果,它可能对理解导致白酒的肝疾病的可能的机制有用。 | Lijun Zhang Xiaofang Jia Yanling Feng Xia Peng Zhiyong Zhang Wenjiang Zhou Zhanqing Zhang Fang Ma Xiaohui Liu Ye Zheng Pengyuan Yang Zhenghong Yuan | 2011 | Acta Biochimica et Biophysica Sinica2011，43，1： | 8 |
| 2 | Generation and selection of immunized Fab phage display library against human B cell lymphoma显示文摘与 B 房间淋巴瘤在病人的管理把单音的同种细胞的抗体用作指向的治疗的赞同为这组病人导致了新治疗选择。由传统的 hybridoma 技术的单音的同种细胞的抗体的生产是昂贵的,并且结果鼠科的抗体经常有触发人的反老鼠抗体(HAMA ) 反应的劣势。因此,噬菌体产生的重组体 Fab 抗体显示技术能是在管理 B 房间淋巴瘤的一种合适的选择。在这研究,我们从与人的 B 淋巴瘤房间使免疫的 BALB/c 老鼠的怒气房间提取了全部的 RNA,并且使用了 RT-PCR 为 kappa 光链和重链的 Fd 碎片放大 cDNAscoding。在适当限制文摘以后,这些 cDNA 碎片连续地被插入到噬菌体构造一个使免疫的 Fab 噬菌体显示图书馆的中间的向量 pComb3H-SS。构造图书馆的差异是近似 1.94x10 (7 ) 。跟随五轮简历 panning,可溶的 Fab 抗体从 ELISA 识别的积极克隆被生产。从八积极克隆, FabC06, FabC21, FabC43 andFabC59 为顺序分析被选择。在氨基酸顺序的水平,可变的重域(V (H)) 和可变的轻域(V (L)) 被发现分享 88-92% 和 89-94% 相同,序列分别地由相应鼠科的细菌行基因编码了。而且,有 B 房间淋巴瘤的膜蛋白质的反应被 immunohistochemistryand 表明西方的弄污。这些使免疫的 Fab 抗体可以为 B 房间淋巴瘤的进一步的学习提供一个珍贵工具并且能也贡献疾病治疗的改进。 | Yongmei Shen Xiaochun Yang Ningzheng Dong Xiaofang Xie Xia Bai Yizhen Shi | 2007 | Cell Research2007，17，7： | 7 |
| 3 | Development and validation of a liquid chromatography-mass spectrometry metabonomic platform in human plasma of liver failure caused by hepatitis B virus显示文摘 | Lijun Zhang Xiaofang Jia Xia Peng Qiang Ou Zhengguo Zhang Chao Qiu Yamin Yao Fang Shen Hua Yang Fang Ma Jiefei Wang Zhenghong Yuan | 2010 | Acta Biochimica et Biophysica Sinica2010，42，10： | 6 |
| 4 | Dataset of the plasma membrane proteome of nasopharyngeal carcinoma cell line HNE1 for uncovering protein function显示文摘鼻咽的癌(NPC ) 是在南部的中国和东南亚洲的一个通常发生的肿瘤。当前的学习集中了于为 NPC 房间线 HNE1 的外部、不可分的蛋白质开发一个广泛的分析方法。外部膜蛋白质被 biotinylated 丰富提取, 0.1 M Na 2 公司 3, 和 H 2 O。不可分或全部的血浆膜部分用 30% Percoll 密度等级 centrifugation 被准备与或没有 0.1 M Na 2 公司 3 处理并且由西方的污点分析评估了。蛋白质受到与双人脚踏车团 spectrometry,与双人脚踏车团 spectrometry 相结合的钠 dodecyl sulfate-polyacrylamide 胶化电气泳动,和猎枪分析相结合的二维的电气泳动。我们分别地从外部、不可分、全部的血浆膜部分识别了 371, 180,和 702 蛋白质。总共, 848 非冗余的蛋白质(534 个组) 被识别。有约束力、催化、结构的分子是主要的班。除了 NPC 房间的已知的房间表面标记,分析在癌症房间是特征的疾病小径揭示了涉及多重发信号房间的小径的 311 蛋白质和 25 蛋白质。由寻找,差别表示了蛋白质数据库(http://gffzze0f623e48f094866hnpuwbcbfx5qk6uxx.ffgz.tsg.suse.edu.cn/depd/index.jsp ) , 199 蛋白质被发现是在以前的癌症 proteome 研究表示的差别。671 蛋白质蛋白质相互作用网络被获得,包括在这个工作的 178 识别蛋白质。五蛋白质的血浆膜本地化被免疫学的技术证实,验证这 proteomic 策略。我们的学习能为理解 NPC 的分子的机制提供一些帮助。 | Lijun Zhang Xiaofang Jia Xiaohui Liu Tingting Sheng Rui Cao Quanyuan He Zhen Liu Xia Peng JixianXiong Pengfei Zhang Ni Shi Songping Liang | 2008 | Acta Biochimica et Biophysica Sinica2008，40，1： | 5 |
| 5 | Clinical manifestations and pathogen characteristics in children admitted for suspected COVID-19显示文摘Coronavirus disease 2019(COVID-19),which is caused by severe acute respiratory syndrome coronavirus-2(SARS-CoV-2),has spread around the world.However,approaches to distinguish COVID-19 from pneumonia caused by other pathogens have not yet been reported.We retrospectively analyzed the clinical data of 97 children with probable COVID-19.A total of 13(13.4%)patients were confirmed positive for SARS-CoV-2 infection by nucleic acid RT-PCR testing,and 41(42.3%)patients were found to be infected with other pathogens.Notably,no pathogen was detected in 43(44.3%)patients.Among all patients,25(25.8%)had familial cluster exposure history,and 52(53.6%)had one or more coexisting conditions.Fifteen(15.5%)patients were admitted or transferred to the PICU.In the 11 confirmed COVID-19 cases,5(45.5%)and 7(63.6%)were positive for IgM and IgG against SARS-CoV-2,respectively.In 22 patients with suspected COVID-19,1(4.5%)was positive for IgG but negative for IgM.The most frequently detected pathogen was Mycoplasma pneumonia(29,29.9%).One patient with confirmed COVID-19 died.Our results strongly indicated that the detection of asymptomatic COVID-19 or coexisting conditions must be strengthened in pediatric patients.These cases may be difficult to diagnose as COVID-19 unless etiologic analysis is conducted.A serologic test can be a useful adjunctive diagnostic tool in cases where SARS-CoV-2 infection is highly suspected but the nucleic acid test is negative. | Xiaofang Cai Hanlan Jiang Simin Zhang Shengying Xia Wenhui Du Yaoling Ma Tao Yu Wenbin Li | 2020 | Frontiers of Medicine2020，14，6： | 2 |
| 6 | Landslide Monito- ring in the Three Gorges Area Using D-InSAR and Corner Reflectors 显示文摘 | Xia Ye Horaeio K Guo Xiaofang | 2004 | Photogrammetric Engineering and Remote Sensing2004，70，10： | 1 |
| 7 | Chlamydia trachomatis infection in the genital tractis associated with inflammation and hypospermia inthe infertile male of China显示文摘Chlamydia trachomatis (CT) infection is the most prevalent sexually transmitted bacterial disease worldwide. However, unlikethat in female infertility, the role of CT infection in male infertility remains controversial. The objective of this retrospective studywas to explore the impacts of CT infection in the genital tract on sperm quality, sperm acrosin activity, antisperm antibody levels,and inflammation in a large cohort of infertile males in China. A total of 7154 semen samples were collected from infertile malesubjects, 416 of whom were CT positive (CT+ group) and 6738 of whom were CT negative (CT− group), in our hospital betweenJanuary 2016 and December 2018. Routine semen parameters (semen volume, pH, sperm concentration, viability, motility,morphology, etc.), granulocyte elastase levels, antisperm antibody levels, and sperm acrosin activity were compared between theCT+ and CT− groups. Our results showed that CT infection was significantly correlated with an abnormally low semen volume, aswell as an increased white blood cell count and granulocyte elastase level (all P < 0.05) in the semen of infertile males;otherroutine semen parameters were not negatively impacted. The antisperm antibody level and sperm acrosin activity were not affectedby CT infection. These findings suggested that CT infection might contribute to inflammation and hypospermia but does not impairsperm viability, motility morphology, and acrosin activity or generate antisperm antibodies in the infertile males of China. | Hua Zhou Shunhong Wu Xiaohua Tang Guanqing Zhou Jingru Yuan Qing Li Yaoyong Chen Xia Xu Xiaofang Sun Detu Zhu Yumei Luo | 2022 | Asian Journal of Andrology2022，24，1： | 1 |
| 8 | Differential SAR Interferometry Using Corner Refletors 显示文摘 | Ye Xia Hermann Kaufmann and Xiaofang Guo | 2002 | IEEE2002，，： | 1 |
| 9 | The role of N--methy--D--aspartate receptor subunit NR2B in the spinal cord in cancer pain显示文摘 | Juan Zhang ZhengLiong Ma JunHua Wang XiaoFang Zhou XiaoPing Gu YanQing Jin XiaoPing Xia Qin Gao FengMei Mei | 2009 | 麻醉与监护论坛2009，16，3： | 1 |
| 10 | Embodied water analysis for Hebei Province, China by input-output modelling显示文摘与加速协调了 Beijing-Tianjin-Hebei 区域的开发,地区性的经济集成作为国家策略被认出。作为在窘境的水缺乏地方 Hebei 省, Hebei 省平衡水资源以及在转变充分利用它的唯一的优点到持续开发具有批评重要性。到我们的知识,相关的表达的水财务分析为北京和天津被进行了,当有 Hebei 的焦点的类似的工作没被发现时。在这份报纸,用为 Hebei 省可得到的最完全、最近的统计,在 Hebei 省的表达的水使用详细被分析。把分析基于输入产量,它为水资源介绍系统财务框架的一个完全的集合。另外,表达的水紧张的一个数据库被建议它对中间的输入和最后的需求适用。结果建议在最后的需求的表达的水的全部的数量是水在城市的家庭消费报道表达了为的 106.2 亿 m 3, 多于半。当一张网表达了水进口商,在 Hebei 省在商品贸易表达的水是 172 亿 m 3 。这个工作的结果暗示为水保存调整工业结构和贸易政策是特别地迫切的,到升级技术并且到改进水利用。作为结果,在 Hebei 省浇缺乏减轻,在省以内调整水使用的平衡具有关键重要性,因此在各种各样的工业部门平衡水分发。 | Siyuan LIU Mengyao HAN Xudong WU Xiaofang WU Zhi LI Xiaohua XIA Xi JI | 2018 | Frontiers of Earth Science2018，12，1： | 1 |
| 11 | Pectin Methylesterases Enhance Root Cell Wall Phosphorus Remobilization in Rice显示文摘Pectin contributes greatly to cell wall phosphorus(P)remobilization.However,it is currently unclear whether the methylesterification degree of the pectin,which is related to the activity of pectin methylesterases(PMEs),is also involved in this process.Here,we demonstrated that elevated PME activity can facilitate the remobilization of P deposited in the cell wall.P-deficient conditions resulted in the reduction of root cell wall P content.This reduction was more pronounced in Nipponbare than in Kasalath,in company with a significant increment of the PME activity,indicating a possible relationship between elevated PME activity and cell wall P remobilization.This hypothesis was supported by in vitro experiments,as pectin with lower methylesterification degree had higher ability to release inorganic P(Pi)from insoluble FePO_(4).Furthermore,among the 35 OsPME members in rice,only the expression of OsPME14 showed a relationship with PME activity.In addition,transgenic rice lines overexpressing OsPME14 had increased PME activity,released more P from the root cell wall,and more resistant to P deficiency.In conclusion,PMEs enhance P remobilization in P-starved rice by increasing PME activity in Nipponbare,which in turn helps to remobilize P from the cell wall,and thus makes more available P. | WU Qi TAO Ye ZHANG Xiaolong DONG Xiaoying XIA Jixing SHEN Renfang ZHU Xiaofang | 2022 | Rice science2022，29，2： | 0 |
| 12 | Impact of physical activity on the prevalence of hypertension among the older adults in Beijing communities显示文摘This study aims to evaluate the impact of physical activity (PA) on the prevalence of hypertension among older adults in Beijing community. As economy growing rapidly in China, the occurrence of hypertension increases among older people in China as well. Epidemiological studies have shown that physical activity may significantly related to lower risk of hypertension. Taking PA maybe an instructive factor to reduce the risk of being hypertensive. We randomly selected Beijing community residents aged 65 and above (n = 400), collected data comprising level of PA (low, moderate, high), blood pressure, and a host of potentially confounding variables indicated by the literature. Five logistic regression models adjusted for different modifiers were used to estimate the association between hypertension and PA. The prevalence of hypertension was 96.88%, 78.57% and 73.66% among subjects with low, moderate and high level of PA respectively. Lower diastolic BP was observed for elder people with higher level of PA (p<0.01). We observed a strong and statistically significant association between moderate (OR=0.09, 95% CI: 0.01 to 0.74) or high (OR=0.08, 95% CI: 0.01 to 0.57) level of PA and lower risk of hypertension (p < 0.05). PA is a protective factor for hypertension among older Beijing people, which suggests elderly people be encouraged to actively engage in PA, if body conditions permit. | Hui Miao Jun Li Xiaofang Weng Xia Wu Aoyi Deng Jingwen Zhao Ting Cai | 2019 | Advances in Health and Behavior2019，2，1： | 0 |
| 13 | A Dynamic Reputation–based Consensus Mechanism for Blockchain显示文摘In recent years,Blockchain is gaining prominence as a hot topic in academic research.However,the consensus mechanism of blockchain has been criticized in terms of energy consumption and performance.Although Proof-of-Authority(PoA)consensus mechanism,as a lightweight consensus mechanism,is more efficient than traditional Proof-of-Work(PoW)and Proof-of-Stake(PoS),it suffers from the problem of centralization.To this end,on account of analyzing the shortcomings of existing consensus mechanisms,this paper proposes a dynamic reputation-based consensus mechanism for blockchain.This scheme allows nodes with reputation value higher than a threshold apply to become a monitoring node,which can monitor the behavior of validators in case that validators with excessive power cause harm to the blockchain network.At the same time,the reputation evaluation algorithm is also introduced to select nodes with high reputation to become validators in the network,thus increasing the cost of malicious behavior.In each consensus cycle,validators and monitoring nodes are dynamically updated according to the reputation value.Through security analysis,it is demonstrated that the scheme can resist the attacks of malicious nodes in the blockchain network.By simulation experiments and analysis of the scheme,the result verifies that the mechanism can effectively improve the fault tolerance of the consensus mechanism,reduce the time of consensus to guarantee the security of the system. | Xiaofang Qiu Zhi Qin Wunan Wan Jinquan Zhang Jinliang Guo Shibin Zhang Jinyue Xia | 2022 | Computers, Materials & Continua2022，，11： | 0 |
| 14 | A Scalable Double-Chain Storage Module for Blockchain显示文摘With the growing maturity of blockchain technology,its peer-topeer model and fully duplicated data storage pattern enable blockchain to act as a distributed ledger in untrustworthy environments.Blockchain storage has also become a research hotspot in industry,finance,and academia due to its security,and its unique data storage management model is gradually becoming a key technology to play its value in various fields’applications.However,with the increasing amount of data written into the blockchain,the blockchain system faces many problems in its actual implementation of the application,such as high storage space occupation,low data flexibility and availability,low retrieval efficiency,poor scalability,etc.To improve the above problems,this paper combines off-chain storage technology and deduplication technology to optimize the blockchain storage model.Firstly,this paper adopts the double-chain model to reduce the data storage of the major chain system,which stores a small amount of primary data and supervises the vice chain through an Application Programming Interface(API).The vice chain stores a large number of copies of data as well as non-transactional data.Our model divides the vice chain storage system into two layers,including a storage layer and a processing layer.In the processing layer,deduplication technology is applied to reduce the redundancy of vice chain data.Our doublechain storage model with high scalability enhances data flexibility,is more suitable as a distributed storage system,and performs well in data retrieval. | Hui Han Wunan Wan Jinquan Zhang Zhi Qin Xiaofang Qiu Shibin Zhang Jinyue Xia | 2022 | Computers, Materials & Continua2022，，11： | 0 |
| 15 | High-precision Calibration of Camera and IMU on Manipulator for Bio-inspired Robotic System显示文摘Inspired by box jellyfish that has distributed and complementary perceptive system,we seek to equip manipulator with a camera and an Inertial Measurement Unit(IMU)to perceive ego motion and surrounding unstructured environment.Before robot perception,a reliable and high-precision calibration between camera,IMU and manipulator is a critical prerequisite.This paper introduces a novel calibration system.First,we seek to correlate the spatial relationship between the sensing units and manipulator in a joint framework.Second,the manipulator moving trajectory is elaborately designed in a spiral pattern that enables full excitations on yaw-pitch-roll rotations and x-y-z translations in a repeatable and consistent manner.The calibration has been evaluated on our collected visual inertial-manipulator dataset.The systematic comparisons and analysis indicate the consistency,precision and effectiveness of our proposed calibration method. | Yinlong Zhang Wei Liang Sichao Zhang Xudong Yuan Xiaofang Xia Jindong Tan Zhibo Pang | 2022 | Journal of Bionic Engineering2022，19，2： | 0 |
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