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| 1 | Treatment and outcomes of 1041 pediatric patients with neuroblastoma who received multidisciplinary care in China显示文摘Importance:Neuroblastoma is the most common extracranial malignant solid tumor in children.Multidisciplinary care is critical to improving the survival of pediatric patients with neuroblastoma.Objective:To systematically summarize the clinical characteristics of children with neuroblastoma and evaluate their prognosis with multidisciplinary care provided in a single center.Methods:This retrospective study analyzed the clinical data of 1041 patients with neuroblastoma who were diagnosed,treated,and followed-up in the Hematology-Oncology Center of Beijing Children’s Hospital from 2007 to 2019.Results:The median age at diagnosis was 34 months;80.8%of the patients were younger than 5 years of age.Notably,243 patients(23.3%)were classified as low-risk,249 patients(23.9%)were classified as intermediate-risk,and 549(52.7%)were classified as high-risk.Furthermore,956 patients underwent surgical resections;986(94.7%)patients received chemotherapy;and 176 patients with high-risk neuroblastoma received hematopoietic stem cell transplantation.The 5-year event-free survival(EFS)rate was 91.3%and 5-year overall survival(OS)rate was 97.5%in low-risk group;in the intermediate-risk group,these rates were 85.1%and 96.7%,respectively,while they were 37.7%and 48.9%in the high-risk group(P<0.001 for both).The 5-year EFS and OS rates were significantly higher in patients diagnosed between 2015 and 2019 than in patients diagnosed between 2007 and 2014(P<0.001).In total,278 patients(26.7%)exhibited tumor relapse or progression;the median interval until relapse or progression was 14 months.Of the 233 patients who died,83%died of relapse or progression of neuroblastoma and 4.3%died of therapy-related complications.Interpretation:The 5-year OS rate was low in high-risk patients,compared with low-and intermediate-risk patients.Multidisciplinary care is critical for improvement of survival in pediatric patients with neuroblastoma.Additional treatment strategies should be sought to improve the prognosis of patients with high-risk neuroblastoma. | Yan Su Hong Qin Chenghao Chen Shengcai Wang Shihan Zhang Dawei Zhang Mei Jin Yun Peng Lejian He Xiaoman Wang Suyun Qian Maoquan Qin Ming Ge Fuquan Zhang Qi Zeng Huanmin Wang Xiaoli Ma Xin Ni | 2020 | Pediatric Investigation2020，4，3： | 16 |
| 2 | General structure design and field experiment of pneumatic rice direct-seeder显示文摘In order to meet the requirement of field direct seeding for super hybrid rice:1-3 seeds per hill,a pneumatic rice direct-seeder with six seeding metering devices was designed.Transmission system of pneumatic rice direct-seeder and seed metering device were analyzed in this study.And the pipeline structure and vortex pump were designed and selected.The pressure distribution of the pipeline was tested.The pressure value of each sub-pipeline under the conditions of different main pipeline pressure value was obtained and the reasons for pressure loss were analyzed.The results showed that the variation coefficient between sub-pipelines was less than 5%.The pipeline pressure could be evenly distributed.Further,the field experiments were carried out on super hybrid rice Peizataifeng using two-factor testing,by which the effects of different negative pressure and different forward speed(namely,rotational speed of suction plate)for seeding precision were studied.It found that:(1)the optimal negative pressure was 2 kPa;(2)under the optimal negative pressure,the probabilities of 1-3 seeds per hill for the direct-seeder were 93.41%,95.47%and 97.50%,respectively,when the forward speed of the direct-seeded was high,medium and low speed;(3)the probability of empty hole was less than 2%,which satisfied the field direct seeding requirements of super hybrid rice.Additionally,hill spacing was measured,and the factors affecting the hill spacing were analyzed.The results showed that the fluctuation of hill spacing in the small range will not affect the seeding effect.In this study,a new type of pneumatic rice direct-seeder was designed.The main working components were tested and analyzed.The best parameters of field work were obtained.It provides the basis for the field application of the pneumatic rice precision seeder. | Xing He Wang Zaiman Luo Xiwen Cao Xiaoman Liu Chunbo Zang Ying | 2017 | International Journal of Agricultural and Biological Engineering2017，10，6： | 10 |
| 3 | Two VOZ transcription factors link an E3 ligase and an NLR immune receptor to modulate immunity in rice显示文摘Nucleotide-binding leucine-rich repeat(NLR)proteins play critical roles in plant immunity.However,how NLRs are regulated and activate defense signaling is not fully understood.The rice(Oryza sativa)NLR receptor Piz-t confers broad-spectrum resistance to the fungal pathogen Magnaporthe oryzae and the RING-type E3 ligase AVRPIZ-T INTERACTING PROTEIN 10(APIP10)negatively regulates Piz-t accumulation.In this study,we found that APIP10 interacts with two rice transcription factors,VASCULAR PLANT ONEZINC FINGER 1(OsVOZ1)and OsVOZ2,and promotes their degradation through the 26S proteasome pathway.OsVOZ1 displays transcriptional repression activity while OsVOZ2 confers transcriptional activation activity in planta.The osvoz1 and osvoz2 single mutants display modest but opposite M.oryzae resistance in the non-Piz-t background.However,the osvoz1 osvoz2 double mutant exhibits strong dwarfism and cell death,and silencing of both genes via RNA interference also leads to dwarfism,mild cell death,and enhanced resistance to M.oryzae in the non-Piz-t background.Both OsVOZ1 and OsVOZ2 interact with Piz-t.Double silencing of OsVOZ1 and OsVOZ2 in the Piz-t background decreases Piz-t protein accumulation and transcription,reactive oxygen species-dependent cell death,and resistance to M.oryzae containing AvrPiz-t.Taken together,these results indicate that OsVOZ1 and OsVOZ2 negatively regulate basal defense but contribute positively to Piz-t-mediated immunity. | Jiyang Wang Ruyi Wang Hong Fang Chongyang Zhang Fan Zhang Zeyun Hao Xiaoman You Xuetao Shi Chan Ho Park Kangyu Hua Feng He Maria Bellizzi Kieu Thi Xuan Vo Jong-Seong Jeon Yuese Ning Guo-Liang Wang | 2021 | Molecular Plant2021，14，2： | 8 |
| 4 | A super pan-genomic landscape of rice显示文摘Pan-genomes from large natural populations can capture genetic diversity and reveal genomic complexity.Using de novo longread assembly,we generated a graph-based super pan-genome of rice consisting of a 251-accession panel comprising both cultivated and wild species of Asian and African rice.Our pan-genome reveals extensive structural variations(SVs)and gene presence/absence variations.Additionally,our pan-genome enables the accurate identification of nucleotide-binding leucine-rich repeat genes and characterization of their inter-and intraspecific diversity.Moreover,we uncovered grain weight-associated SVs which specify traits by affecting the expression of their nearby genes.We characterized genetic variants associated with submergence tolerance,seed shattering and plant architecture and found independent selection for a common set of genes that drove adaptation and domestication in Asian and African rice.This super pan-genome facilitates pinpointing of lineage-specific haplotypes for trait-associated genes and provides insights into the evolutionary events that have shaped the genomic architecture of various rice species. | Lianguang Shang Xiaoxia Li Huiying He Qiaoling Yuan Yanni Song Zhaoran Wei Hai Lin Min Hu Fengli Zhao Chao Zhang Yuhua Li Hongsheng Gao Tianyi Wang Xiangpei Liu Hong Zhang Ya Zhang Shuaimin Cao Xiaoman Yu Bintao Zhang Yong Zhang Yiqing Tan Mao Qin Cheng Ai Yingxue Yang Bin Zhang Zhiqiang Hu Hongru Wang Yang Lv Yuexing Wang Jie Ma Quan Wang Hongwei Lu Zhe Wu Shanlin Liu Zongyi Sun Hongliang Zhang Longbiao Guo Zichao Li Yongfeng Zhou Jiayang Li Zuofeng Zhu Guosheng Xiong Jue Ruan Qian Qian | 2022 | Cell Research2022，32，10： | 3 |
| 5 | Identifying candidate genes and patterns of heat-stress response in rice using a genome-wide association study and transcriptome analyses显示文摘Because high temperatures impair rice production,it is desirable to elucidate the regulatory mechanisms involved in rice response to heat stress.The objectives of this study were to identify candidate genes and characterize their response patterns during rice adaptation to high temperatures at the seedling stage.Ten heat-associated quantitative-trait loci were identified in a genome-wide association study.Comparison of transcript abundances in heat-sensitive and heat-tolerant rice pools under heat stress revealed approximately 400 differentially expressed genes.The expression of genes from heatsensitive accessions changed more than those from heat-tolerant accessions under heat stress.Alternative splicing(AS)events responded to heat stress in rice.The types of AS variants significant different between the heat-sensitive and heat-tolerant accessions.Expression patterns differing between the heat-sensitive and heat-tolerant accessions were identified for genes known to be involved in heat stress.We identified eleven genes associated with rice heat stress response.These genes could be pyramided to breed heat-tolerant rice accessions. | Yingxue Yang Chao Zhang De Zhu Huiying He Zhaoran Wei Qiaoling Yuan Xiaoxia Li Xu Gao Bin Zhang Hongsheng Gao Bo Wang Shuaimin Cao Tianyi Wang Yuhua Li Xiaoman Yu Longbiao Guo Guanjing Hu Qian Qian Lianguang Shang | 2022 | The Crop Journal2022，10，6： | 1 |
| 6 | Pretreatment of cyclohexanone manufacturing wastewater by iron- carbon micro-electrolysis 显示文摘 | Li Dongwei Jiao Binquan He Xiaoman | 2010 | Res J Chem Environ2010，14，： | 1 |
| 7 | A complete assembly of the rice Nipponbare reference genome显示文摘Dear Editor,In 2005,the current commonly used rice reference genome(Oryza sativa ssp.japonica cv.Nipponbare)was initially released by the International Rice Genome Sequencing Project(International Rice Genome Sequencing Project,2005).Thereafter,the reference genome was further updated in 2013 with improved genome assembly(IRGSP-1.0)and gene annotations(MSU7,RAP-DB)(Kawahara et al.,2013;Sakai et al.,2013).In the past 10 years,this reference has been serving as one of the most important genetic resources for subsequent rice functional genomics efforts.As several rice genomes had been assembled into gapless chromosomes with only 2–5 telomeres absent(Li et al.,2021;Song et al.,2021;Zhang et al.,2022),the IRGSP1.0 and its annotations still performed as the most widely used reference.However,limitations of sequencing technology and intricate genomic organization led to an under-representation of complex regions in this reference,leaving a total of 72 major gaps(including 19 telomeres),167 minor gaps,and 779 unknown bases(Kawahara et al.,2013),with an estimated length of3%of the genome unsolved. | Lianguang Shang Wenchuang He Tianyi Wang Yingxue Yang Qiang Xu Xianjia Zhao Longbo Yang Hong Zhang Xiaoxia Li Yang Lv Wu Chen Shuo Cao Xianmeng Wang Bin Zhang Xiangpei Liu Xiaoman Yu Huiying He Hua Wei Yue Leng Chuanlin Shi Mingliang Guo Zhipeng Zhang Bintao Zhang Qiaoling Yuan Hongge Qian Xinglan Cao Yan Cui Qianqian Zhang Xiaofan Dai Congcong Liu Longbiao Guo Yongfeng Zhou Xiaoming Zheng Jue Ruan Zhukuan Cheng Weihua Pan Qian Qian | 2023 | Molecular Plant2023，16，8： | 1 |
| 8 | Construction of algal-bacterial consortia using green microalgae Chlorella vulgaris and As(Ⅲ)-oxidizing bacteria:As tolerance and metabolomic profiling显示文摘Bioremediation became a promising technology to resolve arsenic(As)contamination in aquatic environment.Since monoculture such as microalgae or bacteria was sensitive to environmental disturbance and vulnerable to contamination,green microalgae Chlorella vulgaris and arsenite(As(Ⅲ))-oxidizing bacteria Pseudomonas sp.SMS11 were co-cultured to construct algal-bacterial consortia in the current study.The effects of algae-bacteria(A:B)ratio and exposure As(Ⅲ)concentration on algal growth,As speciation and metabolomic profile were investigated.Algal growth arrested when treated with 100 mg/L As(Ⅲ)without the co-cultured bacteria.By contrast,co-cultured with strain SMS11 significantly enhanced As tolerance in C.vulgaris especially with A:B ratio of 1:10.All the As(Ⅲ)in culture media of the consortia were oxidized into As(Ⅴ)on day 7.Methylation of As was observed on day 14.Over 1% and 0.5% of total As were converted into dimethylarsinic acid(DMA)after 21days cultivation when the initial concentrations of As(Ⅲ)were 1 and 10 mg/L,respectively.Metabolomic analysis was further performed to reveal the response of consortia metabolites to external As(Ⅲ).The enriched metabolomic pathways were associated with carbohydrate,amino acid and energy metabolisms.Tricarboxylic acid cycle and glyoxylate and dicarboxylate metabolism were upregulated under As stress due to their biological functions on alleviating oxidative stress and protecting cells.Both carbohydrate and amino acid metabolisms provided precursors and potential substrates for energy production and cell protection under abiotic stress.Alterations of the pathways relevant to carbohydrate or amino acid metabolism were triggered by energy requirement. | Xiaoman He Guobing Lin Jiayuan Zeng Zhaoguang Yang Lin Wang | 2024 | Journal of Environmental Sciences2024，，5： | 0 |
| 9 | Identification of natural allelic variation in TTL1 controlling thermotolerance and grain size by a rice super pan-genome∞显示文摘Continuously increasing global temperatures present great challenges to food security.Grain size,one of the critical components determining grain yield in rice(Oryza sativa L.),is a prime target for genetic breeding.Thus,there is an immediate need for genetic improvement in rice to maintain grain yield under heat stress.However,quantitative trait loci(QTLs)endowing heat stress tolerance and grain size in rice are extremely rare.Here,we identified a novel negative regulator with pleiotropic effects,Thermo‐Tolerance and grain Length 1(TTL1),from the super pan‐genomic and transcriptomic data.Loss‐of‐function mutations in TTL1 enhanced heat tolerance,and caused an increase in grain size by coordinating cell expansion and proliferation.TTL1 was shown to function as a transcriptional regulator and localized to the nucleus and cell membrane.Furthermore,haplotype analysis showed that hapL and hapS of TTL1 were obviously correlated with variations of thermotolerance and grain size in a core collection of cultivars.Genome evolution analysis of available rice germplasms suggested that TTL1 was selected during domestication of the indica and japonica rice subspecies,but still had much breeding potential for increasing grain length and thermotolerance.These findings provide insights into TTL1 as a novel potential target for the development of high‐yield and thermotolerant rice varieties. | Yarong Lin Yiwang Zhu Yuchao Cui Hongge Qian Qiaoling Yuan Rui Chen Yan Lin Jianmin Chen Xishi Zhou Chuanlin Shi Huiying He Taijiao Hu Chenbo Gu Xiaoman Yu Xiying Zhu Yuexing Wang Qian Qian Cuijun Zhang Feng Wang Lianguang Shang | 2023 | Journal of Integrative Plant Biology2023，65，12： | 0 |
| 10 | Ionome profiling and arsenic speciation provide evidence of arsenite detoxification in rice by phosphate and arsenite-oxidizing bacteria显示文摘Arsenite(As(III))as the most toxic and mobile form is the dominant arsenic(As)species in flooded paddy fields,resulting in higher accumulation of As in paddy rice than other terrestrial crops.Mitigation of As toxicity to rice plant is an important way to safeguard food production and safety.In the current study,As(III)-oxidizing bacteria Pseudomonas sp.strain SMS11 was inoculated with rice plants to accelerate conversion of As(III)into lower toxic arsenate(As(V)).Meanwhile,additional phosphate was supplemented to restrict As(V)uptake by the rice plants.Growth of rice plant was significantly inhibited under As(III)stress.The inhibition was alleviated by the introduction of additional P and SMS11.Arsenic speciation showed that additional P restricted As accumulation in the rice roots via competing common uptake pathways,while inoculation with SMS11 limited As translocation from root to shoot.Ionomic profiling revealed specific characteristics of the rice tissue samples from different treatment groups.Compared to the roots,ionomes of the rice shoots were more sensitive to environmental perturbations.Both extraneous P and As(III)-oxidizing bacteria SMS11 could alleviate As(III)stress to the rice plants through promoting growth and regulating ionome homeostasis. | Guobing Lin Xiaoman He Jiayuan Zeng Zhaoguang Yang Lin Wang | 2023 | Journal of Environmental Sciences2023，，6： | 0 |
| 11 | A centromere map based on super pan-genome highlights the structure and function of rice centromeres显示文摘Rice(Oryza sativa)is a significant crop worldwide with a genome shaped by various evolutionary factors.Rice centromeres are crucial for chromosome segregation,and contain some unreported genes.Due to the diverse and complex centromere region,a comprehensive understanding of rice centromere structure and function at the population level is needed.We constructed a high-quality centromere map based on the rice super pangenome consisting of a 251-accession panel comprising both cultivated and wild species of Asian and African rice.We showed that rice centromeres have diverse satellite repeat CentO,which vary across chromosomes and subpopulations,reflecting their distinct evolutionary patterns.We also revealed that long terminal repeats(LTRs),especially young Gypsy-type LTRs,are abundant in the peripheral CentO-enriched regions and drive rice centromere expansion and evolution.Furthermore,high-quality genome assembly and complete telomere-to-telomere(T2T)reference genome enable us to obtain more centromeric genome information despite mapping and cloning of centromere genes being challenging.We investigated the association between structural variations and gene expression in the rice centromere.A centromere gene,OsMAB,which positively regulates rice tiller number,was further confirmed by expression quantitative trait loci,haplotype analysis and clustered regularly interspaced palindromic repeats(CRISPR)/CRISPR-associated protein9 methods.By revealing the new insights into the evolutionary patterns and biological roles of rice centromeres,our finding will facilitate future research on centromere biology and crop improvement. | Yang Lv Congcong Liu Xiaoxia Li Yueying Wang Huiying He Wenchuang He Wu Chen Longbo Yang Xiaofan Dai Xinglan Cao Xiaoman Yu Jiajia Liu Bin Zhang Hua Wei Hong Zhang Hongge Qian Chuanlin Shi Yue Leng Xiangpei Liu Mingliang Guo Xianmeng Wang Zhipeng Zhang Tianyi Wang Bintao Zhang Qiang Xu Yan Cui Qianqian Zhang Qiaoling Yuan Noushin Jahan Jie Ma Xiaoming Zheng Yongfeng Zhou Qian Qian Longbiao Guo Lianguang Shang | 2024 | Journal of Integrative Plant Biology2024，66，2： | 0 |
| 12 | Robust,self-adhesive,and low-contact impedance polyvinyl alcohol/polyacrylamide dual-network hydrogel semidry electrode for biopotential signal acquisition显示文摘Herein,we fabricated a flexible semidry electrode with excellent mechanical performance,satisfactory self-adhesiveness,and low-contact impedance using physical/chemical crosslinked polyvinyl alcohol/polyacrylamide dual-network hydrogels(PVA/PAM DNHs)as an efficient saline reservoir.The resultant PVA/PAM DNHs showed admirable adhesive and compliance to the hairy scalp,facilitating the establishment of a robust electrode/skin interface for biopotential signal transmission.Moreover,the PVA/PAM DNHs steadily released trace saline onto the scalp to achieve the minimized potential drift(1.47±0.39 mV/min)and low electrode–scalp impedance(18.2±8.9 kΩ@10 Hz).More importantly,the application feasibility of real-world brain−computer interfaces(BCIs)was preliminarily validated by 10 participants using two classic BCI paradigms.The mean temporal cross-correlation coefficients between the semidry and wet electrodes in the eyes open/closed and the N200 speller paradigms are 0.919±0.054 and 0.912±0.050,respectively.Both electrodes demonstrate anticipated neuroelectrophysiological responses with similar patterns.This semidry electrode could also effectively capture robust P-QRS-T peaks during electrocardiogram recording.Considering their outstanding advantages of fast setup,user-friendliness,and robust signals,the proposed PVA/PAM DNH-based electrode is a promising alternative to wet electrodes in biopotential signal acquisition. | Guangli Li Ying Liu Yuwei Chen Yonghui Xia Xiaoman Qi Xuan Wan Yuan Jin Jun Liu Quanguo He Kanghua Li Jianxin Tang | 2024 | SmartMat2024，5，2： | 0 |
| 13 | Effects of Seed Soaking with Paclobutrazol on Early Tillering and Endogenous Hormone Contents of Sugarcane Seedlings显示文摘[Objective] The paper was to explore the effect of seed soaking with paclobutrazol( PP333) on early tillering and endogenous hormone content of sugarcane seedlings.[Method] With the sugarcane variety ROC22 as the experimental material,seeds were soaked in different concentrations of PP333. The number and growth of tiller were investigated,and the contents of endogenous hormones IAA,CTK,ABA and GA3 at two-leaf,four-leaf and six-leaf stages of sugarcane seedlings were measured,to study the effects of seed soaking with PP333 on early tillering and endogenous hormone contents of sugarcane seedlings.[Result] Seed soaking with PP333 could effectively improve and advance tillering occurrence of sugarcane seedlings. The suitable PP333 concentration for sugarcane tillering occurrence,growth and development was 50 mg/L. Seed soaking with PP333 significantly increased the CTK and ABA contents in leaves of sugarcane seedlings,and decreased IAA and GA3 contents. [Conclusion]The paper will provide a theoretical basis for application of PP333 in sugarcane production. | Liu Junxian Li Song Tan Fang Xiong Faqian Wang Xiaoman Guo Wenchan Liu Xin He Yibo Wu Kaichao Liu Limin Liu Hongjian Yu Kunxing Dan Ming Lu Manman | 2017 | Plant Diseases and Pests2017，8，1： | 0 |
| 14 | RRCNN: Request Response-Based Convolutional Neural Network for ICS Network Traffic Anomaly Detection显示文摘Nowadays,industrial control system(ICS)has begun to integrate with the Internet.While the Internet has brought convenience to ICS,it has also brought severe security concerns.Traditional ICS network traffic anomaly detection methods rely on statistical features manually extracted using the experience of network security experts.They are not aimed at the original network data,nor can they capture the potential characteristics of network packets.Therefore,the following improvements were made in this study:(1)A dataset that can be used to evaluate anomaly detection algorithms is produced,which provides raw network data.(2)A request response-based convolutional neural network named RRCNN is proposed,which can be used for anomaly detection of ICS network traffic.Instead of using statistical features manually extracted by security experts,this method uses the byte sequences of the original network packets directly,which can extract potential features of the network packets in greater depth.It regards the request packet and response packet in a session as a Request-Response Pair(RRP).The feature of RRP is extracted using a one-dimensional convolutional neural network,and then the RRP is judged to be normal or abnormal based on the extracted feature.Experimental results demonstrate that this model is better than several other machine learning and neural network models,with F1,accuracy,precision,and recall above 99%. | Yan Du Shibin Zhang Guogen Wan Daohua Zhou Jiazhong Lu Yuanyuan Huang Xiaoman Cheng Yi Zhang Peilin He | 2023 | Computers, Materials & Continua2023，，6： | 0 |
| 15 | Metabolomics analysis of poly(L-lactic acid)nanofibers’performance on PC12 cell differentiation显示文摘The aim of this article is to reveal the influence of aligned/random poly(L-lactic acid)(PLLA)nanofibers on PC12 cell differentiation from the perspective of metabolic level.First,three materials-PLLA aligned nanofibers(PLLA AF),PLLA random nanofibers(PLLA RF)and PLLA films(control)-were prepared by electrospinning and spin coating.Their surface morphologies were characterized.Subsequently,the cell viability,cell morphology and neurite length of PC12 cells on the surface of the three materials were evaluated,indicating more neurites in the PLLA RF groups but the longer average neurite length in the PLLA AF groups.Next,the metabolite profiles of PC12 cells cultured on the surface of the three nanofibers after 12 h,24 h and 36 h showed that,compared with the control,51,48 and 31 types of differential metabolites were detected at the three time points among the AF groups,respectively;and 56,45 and 41 types among the RF groups,respectively.Furthermore,the bioinformatics analysis of differential metabolites identified two pathways and three metabolites critical to PC12 cell differentiation influenced by the nanofibers.In addition,the verification experiment on critical metabolites and metabolic pathways were performed.The integrative analysis combining cytology,metabolomics and bioinformatics approaches revealed that though both PLLA AF and RF were capable of stimulating the synthesis of neurotransmitters,the PLLA AF were more beneficial for PC12 cell differentiation,whereas the PLLA RF were less effective. | Xiaoman Su Yan Huang Rong Chen Yiwen Zhang Meichen He Xiaoying Lu | 2021 | Regenerative Biomaterials2021，8，4： | 0 |
| 16 | Assembly of JAZ–JAZ and JAZ–NINJA complexes in jasmonate signaling显示文摘Jasmonates(JAs)are plant hormones with crucial roles in development and stress resilience.They activate MYC transcription factors by mediating the proteolysis of MYC inhibitors called JAZ proteins.In the absence of JA,JAZ proteins bind and inhibit MYC through the assembly of MYC–JAZ–Novel Interactor of JAZ(NINJA)–TPL repressor complexes.However,JAZ and NINJA are predicted to be largely intrinsically unstructured,which has precluded their experimental structure determination.Through a combination of biochemical,mutational,and biophysical analyses and AlphaFold-derived ColabFold modeling,we characterized JAZ–JAZ and JAZ–NINJA interactions and generated models with detailed,high-confidence domain interfaces.We demonstrate that JAZ,NINJA,and MYC interface domains are dynamic in isolation and become stabilized in a stepwise order upon complex assembly.By contrast,most JAZ and NINJA regions outside of the interfaces remain highly dynamic and cannot be modeled in a single conformation.Our data indicate that the small JAZ Zinc finger expressed in Inflorescence Meristem(ZIM)motif mediates JAZ–JAZ and JAZ–NINJA interactions through separate surfaces,and our data further suggest that NINJA modulates JAZ dimerization.This study advances our understanding of JA signaling by providing insights into the dynamics,interactions,and structure of the JAZ–NINJA core of the JA repressor complex. | X.Edward Zhou Yaguang Zhang Jian Yao Jie Zheng Yuxin Zhou Qing He Javier Moreno Vinh QLam Xiaoman Cao Koichi Sugimoto Leidy Vanegas-Cano Leena Kariapper Kelly Suino-Powell Yuanye Zhu Scott Novick Patrick R.Griffin Feng Zhang Gregg A.Howe Karsten Melcher | 2023 | Plant Communications2023，4，6： | 0 |
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