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| 1 | Latest Progress of the Chinese Meteorological Satellite Program and Core Data Processing Technologies显示文摘In this paper,the latest progress,major achievements and future plans of Chinese meteorological satellites and the core data processing techniques are discussed.First,the latest three FengYun(FY)meteorological satellites(FY-2H,FY-3D,and FY-4A)and their primary objectives are introduced Second,the core image navigation techniques and accuracies of the FY meteorological satellites are elaborated,including the latest geostationary(FY-2/4)and polar-orbit(FY-3)satellites.Third,the radiometric calibration techniques and accuracies of reflective solar bands,thermal infrared bands,and passive microwave bands for FY meteorological satellites are discussed.It also illustrates the latest progress of real-time calibration with the onboard calibration system and validation with different methods,including the vicarious China radiance calibration site calibration,pseudo invariant calibration site calibration,deep convective clouds calibration,and lunar calibration.Fourth,recent progress of meteorological satellite data assimilation applications and quantitative science produce are summarized at length.The main progress is in meteorological satellite data assimilation by using microwave and hyper-spectral infrared sensors in global and regional numerical weather prediction models.Lastly,the latest progress in radiative transfer,absorption and scattering calculations for satellite remote sensing is summarized,and some important research using a new radiative transfer model are illustrated. | Peng ZHANG Qifeng LU Xiuqing HU Songyan GU Lei YANG Min MIN Lin CHEN Na XU Ling Sun Wenguang BAI Gang MA Di XIAN | 2019 | Advances in Atmospheric Sciences2019，36，9： | 26 |
| 2 | Discovery of biclonal origin and a novel oncogene SLC12A5 in colon cancer by single-cell sequencing显示文摘单个房间的定序是为描出的一个强大的工具为个性化的癌症管理的同种细胞的关系和识别关键司机基因。这里,我们执行了结肠癌的一个盒子的单个房间的定序的分析。人口遗传分析在肿瘤房间人口识别了二独立克隆。主要肿瘤克隆作为早 oncogenic 事件怀有 APC 和 TP53 变化,而次要的克隆包含了优势的 CDC27 和 PABPC1 变化。在这二克隆的次要的克隆支持的 APC 和 TP53 变化的缺席从二细胞的起源被导出。体的变化等位基因频率系列的检查另外 21 个整个织物的定序 exome 的盒子在结肠癌揭示了同种细胞的起源的异质。下次,我们识别了在单个房间的水平显示出变化的高频率,但是在人口水平展出了低流行的变异的基因 SLC12A5。变异的 SLC12A5 的功能的描述在结肠癌揭示了它的潜在的 oncogenic 效果。我们的学习在单个房间的水平支持提供第一条 exome 宽的证据那结肠癌能具有 biclonal 起源,并且建议在一个队的低流行的变化可以也在单个水平起重要 protumorigenic 作用。 | Chang Yu Jun Yu Xlaotlan Yao Wllham KK Wu Youyong Lu Senwel Tang Xiangchun Li Li Bao Xiaoxing Li Yong Hou Renhua Wu Min Jian Ruoyan Chen Fan Zhang Lixia Xu Fan Fan Jun He Qiaoyi Liang Hongyi Wang Xueda Hu Minghui He Xiang Zhang Hancheng Zheng Qibin Li Hanjie Wu Yan Chen Xu Yang Shida Zhu Xun Xu Huanming Yang Jian Wang Xiuqing Zhang Joseph JY Sung Yingrui Li Jun Wang | 2014 | Cell Research2014，，6： | 16 |
| 3 | FY-3E:The First Operational Meteorological Satellite Mission in an Early Morning Orbit显示文摘Fengyun-3 E(FY-3E),the world’s first early-morning-orbit meteorological satellite for civil use,was launched successfully at the Jiuquan Satellite Launch Center on 5 July 2021.The FY-3E satellite will fill the vacancy of the global early-morning-orbit satellite observation,working together with the FY-3C and FY-3D satellites to achieve the data coverage of early morning,morning,and afternoon orbits.The combination of these three satellites will provide global data coverage for numerical weather prediction(NWP)at 6-hour intervals,effectively improving the accuracy and time efficiency of global NWP,which is of great significance to perfect the global earth observing system.In this article,the background and meteorological requirements for the early-morning-orbit satellite are reviewed,and the specifications of the FY-3E satellite,as well as the characteristics of the onboard instrumentation for earth observations,are also introduced.In addition,the ground segment and the retrieved geophysical products are also presented.It is believed that the NWP communities will significantly benefit from an optimal temporal distribution of observations provided by the early morning,mid-morning,and afternoon satellite missions.Further benefits are expected in numerous applications such as the monitoring of severe weather/climate events,the development of improved sampling designs of the diurnal cycle for accurate climate data records,more efficient monitoring of air quality by thermal infrared remote sensing,and the quasicontinuous monitoring of the sun for space weather and climate. | Peng ZHANG Xiuqing HU Qifeng LU Aijun ZHU Manyun LIN Ling SUN Lin CHEN Na XU | 2022 | Advances in Atmospheric Sciences2022，39，1： | 13 |
| 4 | Evidence of Oil Sources and Migration in Triassic-Jurassic Reservoirs in the South Tianhuan Depression of the Ordos Basin, China Based on Analysis of Biomarkers and Nitrogen-Bearing Compounds显示文摘The Ordos Basin is an important intracontinental sedimentary basin in western China for its abundant Mesozoic crude oil resources. The southern part of the Tianhuan Depression is located in the southwestern marginal area of this Basin, in which the Jurassic and Triassic Chang-3 are the main oil-bearing strata. Currently, no consensus has been reached regarding oil source and oil migration in the area, and an assessment of oil accumulation patterns is thus challenging. In this paper, the oil source, migration direction, charging site and migration pathways are investigated through analysis of pyrrolic nitrogen compounds and hydrocarbon biomarkers. Oil source correlations show that the oils trapped in the Jurassic and Chang-3 reservoirs were derived from the Triassic Chang-7 source rocks. The Jurassic and Chang-3 crude oils both underwent distinct vertical migration from deep to shallow strata, indicating that the oils generated by Chang-7 source rocks may have migrated upward to the shallower Chang-3 and Jurassic strata under abnormally high pressures, to accumulate along the sand bodies of the ancient rivers and the unconformity surface. The charging direction of the Jurassic and Chang-3 crude oils is primarily derived from Mubo, Chenhao, and Shangliyuan, which are located northeast of the southern Tianhuan Depression, with oils moving toward the west, southwest, and south. The results show that an integration of biomarker and nitrogen-bearing compound analyses can provide useful information about oil source, migration, and accumulation. | ZHAO Yande YAO Jingli SUN Bainian Duan Yi DONG Chong DENG Xiuqing LIU Luofu | 2014 | Acta Geologica Sinica(English Edition)2014，88，6： | 10 |
| 5 | The Existence and Long-Time Behavior of Weak Solution to Bipolar Quantum Drift-Diffusion Model显示文摘The authors study the existence and long-time behavior of weak solutions to the bipolar transient quantum drift-diffusion model,a fourth order parabolic system.Using semi-discretization in time and entropy estimate,the authors get the global existence of nonnegative weak solutions to the one-dimensional model with nonnegative initial and homogenous Neumann(or periodic)boundary conditions.Furthermore,by a logarithmic Sobolev inequality,it is proved that the periodic weak solution exponentially approaches its mean value as time increases to infinity. | Xiuqing CHEN Li CHEN Huaiyu JIAN | 2007 | Chinese Annals of Mathematics,Series B2007，28，6： | 7 |
| 6 | High-throughput experiments facilitate materials innovation:A review显示文摘Since the Material Genome Initiative(MGI) was proposed, high-throughput based technology has been widely employed in various fields of materials science. As a theoretical guide, material informatics has been introduced based on machine learning and data mining and high-throughput computation has been employed for large scale search, narrowing down the scope of the experiment trials. High-throughput materials experiments including synthesis, processing, and characterization technologies have become valuable research tools to pin down the prediction experimentally, enabling the discovery-to-deployment of advances materials more efficiently at a fraction of cost. This review aims to summarize the recent advances of high-throughput materials experiments and introduce briefly the development of materials design based on material genome concept. By selecting representative and classic works in the past years, various high-throughput preparation methods are introduced for different types of material gradient libraries, including metallic, inorganic materials, and polymers. Furthermore, high-throughput characterization approaches are comprehensively discussed, including both their advantages and limitations. Specifically, we focus on high-throughput mass spectrometry to analyze its current status and challenges in the application of catalysts screening. | LIU Yi Hao HU ZiHeng SUO ZhiGuang HU LianZhe FENG LingYan GONG XiuQing LIU Yi ZHANG JinCang | 2019 | Science China(Technological Sciences)2019，62，4： | 7 |
| 7 | A draft sequence of the rice (Oryza sativa ssp. indica) genome显示文摘The sequence of the rice genome holds fundamental information for its biology, including physiology, genetics, development, and evolution, as well as information on many beneficial phenotypes of economic significance. Using a 'whole genome shotgun' approach, we have pro-duced a draft rice genome sequence of Oryza sativa ssp. in-dica, the major crop rice subspecies in China and many other regions of Asia. The draft genome sequence is constructed from over 4.3 million successful sequencing traces with an accumulative total length of 2214.9 Mb. The initial assembly of the non-redundant sequences reached 409.76 Mb in length, based on 3.30 million successful sequencing traces with a total length of 1797.4 Mb from an indica variant cultivar 93-11, giving an estimated coverage of 95.29% of the rice genome with an average base accuracy of higher than 99%. The coverage of the draft sequence, the randomness of the sequence distribution, and the consistency of BIG-ASSEM-BLER, a custom-designed software package | YU Jun, HU Songnian, WANG Jun,LI Songgang WONG Ka-Shu Gane, LIU Bin,DENG Yajun, DAI Li, ZHOU Yan,ZHANG Xiuqing, CAO Mengliang, LIU Jing,SUN Jiandong , TANG Jiabin, CHEN Yanjiong,HUANG Xiaobing, LIN Wei, YE Chen, TONG Wei,CONG Lijuan, GENG Jianing, HAN Yujun, LI Lin,LI Wei, HU Guangqiang, HUANG Xiangang,LI Wenjie, LI Jian, LIU Zhanwei, LI Long,LIU Jianping, Ql Qiuhui, LIU Jinsong, LI Li,WANG Xuegang, LU Hong, WU Tingling,ZHU Miao, Nl Peixiang, HAN Hua, DONG Wei,REN Xiaoyu, FENG Xiaoli, GUI Peng,LI Xianran, WANG Hao, XU Xin, ZHAI Wenxue,XU Zhao, ZHANG Jinsong, HE Sijie,ZHANG Jianguo, XU Jichen, ZHANG Kunlin,ZHENG Xianwu, DONG Jianhai, ZENG Wanyong,TAO Lin, CHEN Xuewei, HE Jun, LIU Daofeng,TIAN Wei, TIAN Chaoguang, XIA Hongai,LI Gang, GAO Hui, LI Ping, CHEN Wei ,WANG Xudong, ZHANG Yong, HU Jianfei,WANG Jing, LIU Song, YANG Jian,ZHANG Guangyu, XIONG Yuqing, LI Zhijie,MAO Long, ZHOU Chengshu, ZHU Zhen,CHEN Runsheng, HAO Bailin,ZHENG Weimou, CHEN Shouyi, QUO Wei,LI Guojie, LIU Siqi, HUANG Guyang,TAO Ming, WANG Jian, ZHU Lihuang,YUAN Longping& YANG HuanmingBeijing Genomics Institute/Center of Genomics & Bioinformatics, Chinese Academy of Sciences, Beijing 101300, China Hangzhou Genomics Institute/Institute of Bioinformatics of Zhejiang University/Key Laboratory of Bioinformatics of Zhejiang Province, Hangzhou 310007, China Institute of Genetics, Chinese Academy of Sciences, Beijing 100101, China National Hybrid Rice R & D Center, Changsha 410125, China Laboratory of Bioinformatics, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China College of Life Sciences, Peking University, Beijing 100871, China Institute of Theoretical Physics, Chinese Academy of Sciences, Beijing 1Q0080, China Digital China Ltd., Beijing 100080, China Institute of Computing Technology, Chinese Academy of Sciences, Beijing 100080, China Medical College, Xi’an Jiaotong University, Xi’an 710061, ChinaThese authors contributed equally to this work.Corresponding author.Corresponden | 2001 | Chinese Science Bulletin2001，46，23： | 6 |
| 8 | Genetic dissection of rice appearance quality and cooked rice elongation by genome-wide association study显示文摘Appearance and cooked rice elongation are key quality traits of rice. Although some QTL for these traits have been identified, understanding of the genetic relationship between them remains limited. In the present study, large phenotypic variation was observed in 760 accessions from the 3 K Rice Genomes Project for both appearance quality and cooked rice elongation. Most component traits of appearance quality and cooked rice elongation showed significant pairwise correlations, but a low correlation was found between appearance quality and cooked rice elongation. A genome-wide association study identified 74 QTL distributed on all 12 chromosomes for grain length, grain width, length to width ratio, degree of endosperm with chalkiness, rice elongation difference, and elongation index. Thirteen regions containing QTL stably expressed in multiple environments and/or exerting pleiotropic effects on multiple traits were detected. By gene-based association analysis and haplotype analysis, 46 candidate genes, including five cloned genes, and 49 favorable alleles were identified for these 13 QTL. The effect of the candidate gene Wx on rice elongation difference was validated by a transgenic strategy. These results shed light on the genetic bases of appearance quality and cooked rice elongation and provide gene resources for improving rice quality by molecular breeding. | Xianjin Qiu Jing Yang Fan Zhang Yanan Niu Xiuqing Zhao Congcong Shen Kai Chen Sheng Teng Jianlong Xu | 2021 | The Crop Journal2021，9，6： | 5 |
| 9 | Anti-biofilm and remineralization effects of chitosan hydrogel containing amelogeninderived peptide on initial caries lesions显示文摘In this study,we have designed a more clinically powerful anti-caries treatment by applying the amelogenin-derived peptide QP5 to the antibacterial carrier material chitosan in a hydrogel(CS-QP5 hydrogel),and characterized its effects on the inhibition of a cariogenic biofilm and the promotion of the remineralization of the initial caries lesions.The results indicated that the CS-QP5 hydrogel sustainably inhibited the growth of the Streptococcus mutans biofilm,lactic acid production and the metabolic activity over a prolonged period of time.Moreover,the CS-QP5 hydrogel promoted the remineralization of early enamel lesions,which were indicated by surface microhardness(,polarized light microscopy and transverse microradiography.In conclusion,the CS-QP5 hydrogel shows good potential for caries control in the clinic because of its antibacterial effects as well as the remineralization of initial enamel carious lesions even in a biofilm model over a prolonged period of time. | Qian Ren Zhongcheng Li Longjiang Ding Xiuqing Wang Yumei Niu Xi Qin Xuedong Zhou Linglin Zhang | 2018 | Regenerative Biomaterials2018，5，2： | 5 |
| 10 | Spatial distribution patterns of Symplocos congeners in a subtropical evergreen broad-leaf forest of southern China显示文摘Studies on spatial distribution of congeneric species can supplement our understanding of species ecological processes. We analyzed population structure, spatial distribution, intra-and interspecific associations among six Symplocos tree species on a large(50 ha) plot in a subtropical, evergreen broad-leaf forest in southern China using spatial point pattern analysis methods. Our results suggested that the six Symplocos tree species were all clustered at small scales. The aggregation intensity of S. wikstroemiifolia with low abundance but relatively numerous large-diameter trees was much higher than that of the other five Symplocos species that occurred at high abundance but included few large-diameter trees. Spatial associations among the six congeners showed that 12 of 30 pairs were associated positively at small scales, and 13 of 30 pairs were unrelated. For species among different size classes, 79 of 120 pairs were not correlated, and 17 of 120 pairs were associated positively. These results showed insufficient evidence for interspecific competition and congeneric Symplocos species commonly coexist within subtropical plant communities. The spatial patterns of Symplocos species and their correlations changed with size(DBH) class and were simultaneously affected by spatial scales; the intensity of their aggregation and association decreased with increasing area of the sample plot. | Xiuqing Yang Haibing Yan Buhang Li Youzhi Han Bo Song | 2018 | Journal of Forestry Research2018，29，3： | 5 |
| 11 | Gene characterization and molecular pathway analysis of reverse thermosensitive genic male sterility in eggplant(Solanum melongena L.)显示文摘The naturally occurring mutant eggplant line 05ms was identified with reverse thermosensitive genic male sterility(rTGMS),but its temperature-responsive fertility mechanisms remain largely unknown.Here,we studied the flower morphology,anther cellular structure,and genome-wide gene expression of this rTGMS line.Candidate genes for thermosensitive male sterility during the microspore development of 05ms and the temperature-insensitive line S63 under low-temperature(LT)and high-temperature(HT)conditions were identified.Under LT,tapetum cells were vacuolated and had delayed disintegration in 05ms.RNA-seq analysis indicated that DEGs were enriched in the KEGG pathways‘plant hormone signal transduction’,‘starch and sucrose metabolism’,and‘phenylpropanoid biosynthesis’.We identified two genes,4CLL1(Sme2.5_00368.1_g00010.1)and CKI1(Sme2.5_10056.1_g00002.1),which could potentially regulate eggplant anther development and may be candidate genes for rTGMS.Finally,we propose a working model of anther abortion for rTGMS in eggplant.CKI1 responds to LT stress and causes expression changes in genes related to anther development,such as 4CLL1,and the cellular structure of the tapetum becomes abnormal,causing male sterility.The findings of this study explain the underlying molecular mechanisms of male sterility in eggplant rTGMS lines. | Bing Li Xueping Chen Yanrong Wu Aixia Gu Jingjing Zhang Shuangxia Luo Xiurui Gao Jianjun Zhao Xiuqing Pan Shuxing Shen | 2019 | Horticulture Research2019，6，1： | 5 |
| 12 | New Compensation Standard for Land Expropriation in China显示文摘This paper explores a new compensation standard for use in agricultural land conversion in China during the land-expropriation process, based on the market value definition. Assuming identical bargaining power between the buyer and the seller, efficient and equitable compensation is obtained through a market transaction and bargaining negotiation mechanism, under the assumpation of a perfect market. From the state-of-the-art viewpoint of land appraising, a practical approximation of the theoretical optimal compensation will provide an effective solution for China, under the current collective-owned land property rights structure. | Xiuqing Zou Arie J. Oskam | 2007 | China & World Economy2007，15，5： | 5 |
| 13 | Effects of serum containing natural cerebrolysin on glucose-regulated protein 78 and CCAAT enhancer-binding protein homologous protein expression in neuronal PC12 cells following tunicamycin-induced endoplasmic reticulum stress显示文摘BACKGROUND:Glucose-regulated protein 78(GRP78),a marker of endoplasmic reticulum stress,can prolong cell survival.Alternatively,CCAAT enhancer-binding protein homologous protein(CHOP),a transcription factor specific for endoplasmic reticulum stress,can cause cell cycle arrest and cell apoptosis. OBJECTIVE:To study the protective effects of serum containing natural cerebrolysin on endoplasmic reticulum stress in tunicamycin-induced neuronal PC12 cells,and analyze the influence on GRP78 and CHOP expressions. DESIGN,TIME AND SETTING:A parallel controlled study was performed at the Institute of Integrated Western and Traditional Chinese Medicine,Shenzhen Hospital,Southern Medical University,between March 2006 and August 2008. MATERIALS:Adult Sprague-Dawley rats were perfused with natural Cerebrolysin aqueous extract(0.185 g/kg/d) to produce serum containing natural Cerebrolysin.Physiological saline was used to produce blank serum.PC12 cell line was provided by Shanghai Institute of Cell Biology, Chinese Academy of Science.Tunicamycin was provided by Sigma(St.Louis,USA),and natural Cerebrolysin,containing ginseng,rhizoma gastrodiae,and gingko leaf(1:2:2),by Shengzhen Institute of Integrated Western and Traditional Chinese Medicine. METHODS:PC12 cells were treated with DMEM culture media containing 10%blank serum (normal control group),tunicamycin(1μg/mL;model group),and 5%,10%,and 15%serum containing natural cerebrolysin and tunicamycin(1μg/mL;low-,moderate-,and high-dose serum containing natural cerebrolysin groups),for 2 hours. MAIN OUTCOME MEASURES:PC12 cells were treated with tunicamycin for 48 hours after which apoptosis was measured using the TUNEL method to calculate apoptotic index.GRP78 expression was detected using immunocytochemistry.After 24 hours of treatment with tunicamycin,GRP78 and CHOP mRNA expressions were measured using RT-PCR. RESULTS:The apoptotic index and CHOP mRNA expression were in the model group and three cerebrolysin groups were significantly increased when compared to the normal control group(P<0.05).In contrast,GRP78 mRNA and protein expressions were significantly decreased(P<0.05). CONCLUSION:Serum containing natural cerebrolysin significantly reduced apoptosis in neuronal PC12 cells following tunicamycin-induced endoplasmic reticulum stress.These results may be related to an up-regulation of GRP78 expression and down-regulation of CHOP expression,both of which displayed dose-dependent effects. | Zhengzhi Wu Ming Li Andrew C.J. Huang O Xiuqing Jia Yinghong Li Manyin Chen | 2009 | Neural Regeneration Research2009，4，2： | 4 |
| 14 | FY-3A/MERSI, ocean color algorithm, products and demonstrative applications显示文摘A medium resolution spectral imager (MERSI) on-board the first spacecraft of the second generation of China's polar-orbit meteorological satellites FY-3A, is a MODIS-like sensor with 20 bands covering visible to thermal infrared spectral region. FY-3A/MERSI is capable of making continuous global observations, and ocean color application is one of its main targets. The objective is to provide information about the ocean color products of FY-3A/MERSI, including sensor calibration, ocean color algorithms, ocean color products validation and applications. Although there is a visible on-board calibration device, it cannot realize the on-board absolute radiometric calibration in the reflective solar bands. A multisite vicarious calibration method is developed, and used for monitoring the in-flight response change and providing post-launch calibration coefficients updating. FY-3A/MERSI ocean color products consist of the water-leaving reflectance retrieved from an atmospheric correction algorithm, a chlorophyll a concentration (CHL1) and a pigment concentration (PIG1) from global empirical models, the chlorophyll a concentration (CHL2), a total suspended mater concentration (TSM) and the absorption coefficient of CDOM and NAP (YS443) from China's regional empirical models. The atmospheric correction algorithm based on lookup tables and ocean color components concentration estimation models are described. By comparison with in situ data, the FY-3A/MERSI ocean color products have been validated and preliminary results are presented. Some successful ocean color applications such as algae bloom monitoring and coastal suspended sediment variation have demonstrated the usefulness of FY-3A/MERSI ocean color products. | SUN Ling GUO Maohua ZHU Jianhua HU Xiuqing SONG Qingjun | 2013 | Acta Oceanologica Sinica2013，32，5： | 4 |
| 15 | An α-expansin, VfEXPA1, is involved in regulation of stomatal movement in Vicia faba L.显示文摘The wall loosening of guard cells differs from other types of plant cells. However, the regulation of wall loosening during stomatal movement is poorly understood. VfEXPA1 is an α-expansin gene cloned from Vicia faba epidermal strips. Expression of VfEXPA1 is regulated by darkness and submergence, and is not affected by light and abscisic acid (ABA). In situ hybridization showed that VfEXPA1 is expressed primarily in the guard cells. Overexpression of VfEXPA1 in transgenic tobacco accelerated light-induced stomatal opening, and increased both transpiration and photosynthetic rates under favorable growth conditions. Our results indicate the guard cell-expressed expansin VfEXPA1 plays an important role in regulation of stomatal opening. | WEI PengCheng CHEN Su ZHANG XiuQing ZHAO Ping XIONG YanMei WANG WenLong CHEN Jia WANG XueChen | 2011 | Chinese Science Bulletin2011，56，33： | 4 |
| 16 | Rapid growth and properties of large-aperture 98%-deuterated DKDP crystals显示文摘In this paper,a highly deuterated potassium dihydrogen phosphate(DKDP)crystal with sizes up to 318 mm×312 mm×265 mm was grown by the rapid-growth method.The synthesis tank device was specially designed to synthesize a higher deuterium concentration and high-purity DKDP solution.The deuterium content of the as-grown crystal,which was 97.9%,was determined by two methods,including infrared(IR)spectroscopy and thermo-gravimetric analysis(TGA)measurements.The performances of the 97.9%DKDP crystal,including transmission,absorption coefficient,and laserinduced damage threshold(LIDT)were measured.The results indicate that,in the near-infrared band,the transmission of the 97.9%DKDP crystal is higher than that of KDP and 70%DKDP crystals,and the absorption coefficient is lower.The LIDT of the crystal reached 23.2 J·cm^-2(R-on-1,1064 nm,3 ns),which meets the engineering requirements for use in optical applications. | Xumin Cai Xiuqing Lin Guohui Li Junye Lu Ziyu Hu Guozong Zheng | 2019 | High Power Laser Science and Engineering2019，7，3： | 4 |
| 17 | Characteristic analysis of ecosystem service value of water system in Taiyuan urban district based on LUCC显示文摘Changes in the land use and ecosystem service value of the 600 m buffer zone of Jinyang Lake and the 1000 m riparian zone of the Fenhe River in the Taiyuan urban district in China,were studied using satellite image data collected in June 2006 and June 2013.According to the ecosystem services assessment system based on expert knowledge,the ecosystem service value(ESV)per unit area was determined for various land-use types and the value of the water system and its buffer regions in the Taiyuan urban district were determined.And then the relationship between LUCC(Land Use/Cover Change)and ESV was discussed.Moreover,the accuracy and validity of the results was analyzed.The results showed that:(1)the areas of green vegetation and water bodies of Jinyang Lake and its buffer region decreased by 24.96%and 3.22%,respectively,between June 2006 and June 2013,whereas the areas of farmland and built-up land increased by 15.81%and 12.37%,respectively.At Jinyang Lake,the degree of dynamic change for green vegetation,water bodies,farmland,built-up land,and unused land was−0.13,−0.02,0.17,0.09,and−0.05,respectively.In the Fenhe River’s buffer region,the areas of green vegetation,farmland and unused land decreased by 1.36%,6.36%and 0.3%,respectively,but those of built-up land and water bodies increased by 1.41%and 6.61%,respectively.Their degree of dynamic change was−0.03,−0.02,−0.04,0.01 and 0.32,respectively;(2)In 2006,the landscape fragmentation indices(LFI)of Jinyang Lake buffer of 0-200 m zone were greater than 2,but those of 200-600 m buffer zone were less than 0.3,the water-body index not included.However,in 2013,the landscape fragmentation indices of green vegetation and water bodies of 0-600 m buffer zone were both high,meaning that the land-use pattern had improved.The fragmentation index of green vegetation of the 0-200 m buffer increased from 0.1(the minimum for the year)to 1.7(the maximum for the year)during the seven years of this study along the Fenhe River.The fragmentation index of built-up land increased from 0.007 to 0.01 in the 200-500 m buffer and from 0.007 to 0.06 in the 500-1000 m buffer.(3)The total ecosystem service value of Jinyang Lake and its buffer region decreased by 22.673%,whereas during the same period,that of the Fenhe River and its buffer region increased by 41.345%.The total ecosystem service value of the water bodies and their buffer regions in the Taiyuan urban area increased by 13.725%overall.(4)The change rate of the ecosystem service value of the 0-600 m buffer region decreased by 1.579%at Jinyang Lake,while that of the 0-1000 m buffer region increased 5.079%at the Fenhe River over the seven years. | Liu Ping Zheng Xiuqing Chen Junfeng Zhang Qing Li Jingling Wu Bo | 2016 | International Journal of Agricultural and Biological Engineering2016，9，1： | 3 |
| 18 | Characterization and validation of somatic mutation spectrum to reveal heterogeneity in gastric cancer by single cell sequencing显示文摘Gastric cancer(GC) is a highly heterogeneous disease with multiple cellular types and poor prognosis.However, the cellular evolution and molecular basis of GC at the individual intra-tumor level has not been well demonstrated. We performed single-cell whole exome sequencing to detect somatic singlenucleotide variants(SNVs) and significantly mutated genes(SMGs) among 34 tumor cells and 9 normal cells from a patient with GC. The Complete Prediction for Protein Conformation(CPPC) approach directly predicting the folding conformation of the protein 3D structure with Protein Folding Shape Code, combined with functional experiments were used to confirm the characterization of mutated SMGs in GC cells. We identified 201 somatic SNVs, including 117 non-synonymous mutations in GC cells. Further analysis identified 24 significant mutated genes(SMGs) in single cells, for which a single amino acid change might affect protein conformation. Among them, two genes(CDC27 and FLG) that were mutated only in single cells but not in the corresponding tumor tissue, were recurrently present in another GC tissue cohort, and may play a potential role to promote carcinogenesis, as confirmed by functional characterization. Our findings showed a mutational landscape of GC at intra-tumor level for the first time and provided opportunities for understanding the heterogeneity and individualized target therapy for this disease. | Lihua Peng Rui Xing Dongbing Liu Li Bao Wenxiang Cheng Hongyi Wang Yuan Yu Xiaofeng Liu Lu Jiang Yan Wu Zhongxue An Qiaoyi Liang Ryong Nam Kim Young Kee Shin Huanming Yang Jian Wang Jun Yu Xiuqing Zhang Xun Xu Jiaan Yang Kui Wu Shida Zhu Youyong Lu | 2019 | Science Bulletin2019，64，4： | 3 |
| 19 | High-performance single-chip exon capture allows accurate whole exome sequencing using the Illumina Genome Analyzer显示文摘Here we present an adaptation of NimbleGen 2.1M-probe array sequence capture for whole exome sequencing using the Illumina Genome Analyzer (GA) platform.The protocol involves two-stage library construction.The specificity of exome enrichment was approximately 80% with 95.6% even coverage of the 34 Mb target region at an average sequencing depth of 33-fold.Comparison of our results with whole genome shot-gun resequencing results showed that the exome SNP calls gave only 0.97% false positive and 6.27% false negative variants.Our protocol is also well suited for use with whole genome amplified DNA.The results presented here indicate that there is a promising future for large-scale population genomics and medical studies using a whole exome sequencing approach. | JIANG Tao YANG Lei JIANG Hui TIAN Geng ZHANG XiuQing | 2011 | Science China(Life Sciences)2011，54，10： | 3 |
| 20 | Profiling of hepatocellular carcinoma neoantigens reveals immune microenvironment and clonal evolution related patterns显示文摘Objective: Neoantigens derived from tumor-specific genomic alterations have demonstrated great potential for immunotherapeutic interventions in cancers. However, the comprehensive profile of hepatocellular carcinoma(HCC) neoantigens and their complex interplay with immune microenvironment and tumor evolution have not been fully addressed.Methods: Here we integrated whole exome sequencing data, transcriptome sequencing data and clinical information of 72 primary HCC patients to characterize the HCC neoantigen profile, and systematically explored its interactions with tumor clonal evolution, driver mutations and immune microenvironments.Results: We observed that higher somatic mutation/neoantigen load was associated with better clinical outcomes and HCC patients could be further divided into two subgroups with distinct prognosis based on their neoantigen expression patterns. HCC subgroup with neoantigen expression probability high(NEP-H) showed more aggressive pathologic features including increased incidence of tumor thrombus(P=0.038), higher recurrence rate(P=0.029),more inclined to lack tumor capsule(P=0.026) and with more microsatellite instability sites(P=0.006). In addition,NEP-H subgroup was also characterized by higher chance to be involved in tumor clonal evolution [odds ratio(OR)=46.7, P<0.001]. Gene set enrichment analysis revealed that upregulation of MYC and its targets could suppress immune responses, leading to elevated neoantigen expression proportion in tumor cells. Furthermore, we discovered an immune escape mechanism that tumors could become more inconspicuous by evolving subclones with less immunogenicity. We observed that smaller clonal mutation clusters with higher immunogenicity in tumor were more likely to involve in clonal evolution. Based on identified neoantigen profiles, we also discovered series of neoantigenic hotspot genes, which could serve as potential actionable targets in future.Conclusions: Our results revealed the landscape of HCC neoantigens and discovered two clinically relevant subgroups with distinct neoantigen expression patterns, suggesting the neoantigen expression should be fully considered in future immunotherapeutic interventions. | Zhenli Li Geng Chen Zhixiong Cai Xiuqing Dong Lei He Liman Qiu Yongyi Zeng Xiaolong Liu Jingfeng Liu | 2021 | Chinese Journal of Cancer Research2021，33，3： | 3 |
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