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| 1 | Down-regulation of Hsp90 could change cell cycle distribution and increase drug sensitivity of tumor cells显示文摘:AIM To construct Hsp90 antisense RNAeukaryotic expression vector, transfect it intoSGC7901 and SGC7901/VCR of MDR-type humangastric cancer cell lines, HCC7402 of humanhepatic cancer and Eel09 of human esophagealcancer cell lines, and to study the cell cycledistribution of the gene transected cells andtheir response to chemotherapeutic drugs.METHODS A I .03kb cDNA sequence of Hsp90Pwas obtained from the primary plasmid phHsp90by EcoR 1 and BamH I nuclease digestion andwas cloned to the EcoR 1 and BamH 1 site ofthe pcDNA by T4DNA ligase and an antisenseorientation of Hsp900 expression vector wasconstructed. The constructs were transfectedwith lipofectamine and positive clones wereselected with G418. The expression of RNA wasdetermined with dot blotting and RNaseprotection assay, and the expression of Hsp90protein determined with Western blot. Cell cycledistribution of the transfectants was analyzedwith flow cytometry, and the drug sensitivity ofthe transfectants to adriamycin (ADR ),vincrinstine (VCR ), mitomycin (MMC ) andcyclophosphamide (CTX ) with MTT andintracellular drug concentration of thetransfectants was determined with flowcytometry.RESULTS In EcoR 1 and BamH I restrictionanalysis, the size and the direction of the clonedsequence of Hsp900 remained what had beendesigned and the gene constructs were namedpcDNA-Hsp90. AH^SGC7901, AH^SGC7901/ VCR,AH-HCC7402 and AH-Eel09 cell clones allexpressed Hsp90 anti--sense RNA. Theexpression of Hsp90 was down--regulated in AHSGC7901, AH--SGC7901/ VCR, AH-HCC7402 andAH--Eel09 cell clones. Cell cycle distribution waschanged differently. In AH-SGC7901/ VCR andAH-Ec109 cells, G, phase cells were increased; Sphase and G, phase cells were decreased ascompared with their parental cell lines. In AHSGC7901 cell, G, phase cells were decreased, Qphase cells increased and S phase cells were notchanged, and in AH-HCC7402 cells G,, S and qphase cells remained unchanged as comparedwith their parental cell lines. The sensitivity ofAH--SGC7901, AH--SGC7901/ VCR, AH-HCC7402 andAH-Ec109 to chemotherapeutic drugs, thesensitivity ot AH--SGC7901/ VCR to ADR, VCR,MMC and CTX the sensitivity of AH-HCC7402 toADR and VCR, and the sensitivity of Eel09 toADR, VCR and CTX all increased as comparedwith their parental cell lines. The meanfluorescence intensity of ADR in AH--SGC7901,AH-SGC7901/ VCR, AH--HCC7402 and AH-Ec109was also significantly elevated (P< 0. 05).CONCLUSION Down-regulation of HsP90 couldchange cell cycle distribution and increase thedrug sensitivity of tumor cells. | Liu XL Xiao B Yu ZC Guo JC Zhao QC Xu L Shi YQ Fan DM | 1999 | World Journal of Gastroenterology1999，5，3： | 21 |
| 2 | Mechanism of frost heave by film water migration under temperature gradient显示文摘IT is well known that there exists unfrozen film water in frozen soils due to the action of sur-face energy among soil particles and frost heave occurs by water migration under the tempera-ture gradient. The problem is how to determine the amount of frost heave quantitatively. The amount of frost heave induced by ice segregation can be calculated by the following e-quations: | Xu, XZ Wang, JC Zhang, LX Deng, YS Chuvilin, EM Yershov, ED Ishizaki, T Fukuda, M | 1997 | Chinese Science Bulletin1997，42，15： | 6 |
| 3 | A CRACK CLOSURE MODEL FOR BRITTLE ROCK SUBJECTED TO COMPRESSIVE-SHEAR LOADING显示文摘1INTRODUCTIONCompressionshearfractureofrockwasinvestigatedbymanyresearchers,Raoetal[1]studiedthisfailuremodeusingfourpointb... | Chen, F Sun, ZQ Xu, JC | 1999 | 中国有色金属学会会刊:英文版1999，9，2： | 4 |
| 4 | Cell death and endoplasmic reticulum stress: disease relevance and therapeutic opportunities显示文摘 | Kim I Xu W Reed JC | 2008 | Nat Rev Drug Discov2008，7，： | 1 |
| 5 | Identification of differentially expressed genes in human prostate cancer using subtraxtion and microarray显示文摘 | Xu JC Stolk JA Zhang XQ | 2000 | Cancer Res2000，60，6： | 1 |
| 6 | Renalase is a novel, soluble monoamine oxidase that regulates cardiac function and blood pressure显示文摘 | Xu JC Li GY Gary VD | 2005 | J Clin Invest2005，115，5： | 1 |
| 7 | Specific Heat and Interface Energy of Nanostructured ZrAlNiCuCo Alloy显示文摘The specific heat of the nanostructured crystals, the glass, the liquid and the crystals for a ZrAINiCuCo alloy has been measured by using the differential scanning calorimeter. The specific heat value and its temperature coefficient of the nanostructured crystals are larger than that of the relaxed glass and of the crystals. Through measuring crystallization enthalpies and specific heat of the alloy in different states, the enthalpy difFerences among the crystals, the liquid and the nanostructured crystals are calculated and the intedece energies of nanostructured crystals are estimated. The stability and the grain size limit of the nanostructured crystals are discussed. | Jiang, Q Sui, ZX Li, JC Xu, XY | 1997 | Journal of Materials Science & Technology1997，13，4： | 1 |
| 8 | Endoplasmic reticulum stress:cell life and death decisions 显示文摘 | Xu C Bailly-Maitre B Reed JC | 2005 | J Clin Invest2005，115，10： | 1 |
| 9 | Identification of differentially expressed genes in human prostate cancer using subtraction and microarray 显示文摘 | Xu JC Stolk JA Zhang XQ | 2000 | Cancer Res2000，60，6： | 1 |
| 10 | Leptin expression by heterotopic ossificationisolated tissue in rats with Achilles' tenotomy 显示文摘 | Xu JC Wu T Wu GH | 2009 | Saudi Med J2009，30，5： | 1 |
| 11 | Endoplasmic reticulum stress:cell life and death decisions显示文摘 | Xu C Bailly-Maitre B Reed JC | 2005 | J Clin Invest2005，115，10： | 1 |
| 12 | Corticosteroid therapy in IgA nephropathy显示文摘 | Lv JC Xu D Perkovic V | 2012 | J Am Soc Nephrol2012，23，6： | 1 |
| 13 | Endoplasmic reticulum stress:cell life and death decisions 显示文摘 | Xu C Bailly- Maitre B Reed JC | 2005 | J Clin Invest2005，115，10： | 1 |
| 14 | Intimate partner violence against Chinese women: the past, present, and future 显示文摘 | Xu X Campbell JC Zhu FC | 2001 | Trauma Violence Abuse2001，2，4： | 1 |
| 15 | Hobt and boat-derived immonium salts: new and highly efficient coupling reagents for peptide synthesis显示文摘 | LI P XU JC | 2000 | Tetra- hedron Lett2000，41，5： | 1 |
| 16 | Endoplasmic reticulum stress: cell life and death decisions 显示文摘 | Xu C Bailly--Maitre B Reed JC | 2005 | J Clin Invest2005，115，10： | 1 |
| 17 | Jolkinolide B induces apoptosis in M CF-7 cells through inhibition of the PI3K/Akt/m TOR signaling pathw ay显示文摘 | Xu HY Chen ZW Hou JC | 2013 | Oncol Rep2013，29，1： | 1 |
| 18 | An ecological study on dynamics of toxic Microcystis blooms in a eutrophic park pond, Wuhan, China | Cheng, K Zhao, YJ Xu, M Yin, J Xiong, Q Zhou, JC Wang, YZ Zhang, W Wang, JW Liu, ZY Wang, FR | 2002 | Progress in Natural Science:Materials International2002，12，1： | 1 |
| 19 | Predictors of survival after single-ventricle palliation: the impact of right ventricular dominance 显示文摘 | d' Udekem Y Xu MY Galati JC | 2012 | J Am Coll Cardiol2012，59，： | 1 |
| 20 | Endoplasmic reticulum stress:cell life and death decisions显示文摘 | Xu C Bailly-Maitre B Reed JC | | 0，，10： | 1 |
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