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| 1 | The concept of effective emissivity of nonisothermal mixed pixel and its test显示文摘Based on two preconditions, the local thermal equilibrium is satisfied and emissivities donot change with temperature, the concept of component effective emissivity of nonisothermal mixed pixel has been put forward and then the radiant directionality model of nonisothermal mixed pixel constructed from it. Our study shows that the component effective emissivity is associated with geometric structure, optical properties of target, and viewing angle, but does not depend on the component temepratures. The component temperatures can only change the ratio of component radiance to the total radiance of the mixed-pixel. The total effective emissivity of this pixel is the complement of its directional-hemisphere reflectance. After the simulation of component effective emissivity of the discrete cones and continuous vegetation canopy (winter wheat) by the Monte Carlo method, our model of radiant directionality of nonisothermal mixed pixel have been proved by lab and field measurements. | CHEN Liangfu ZHUANG Jiali XU Xiru NIU Zheng ZHANG Renhua XIANG Yueqing | 2000 | Chinese Science Bulletin2000，45，9： | 17 |
| 2 | Ubiquitination of Rheb governs growth factor-induced mTORC1 activation显示文摘Mechanistic target of rapamycin mTOR complex 1 (mTORC1)plays a key role in the integration of various environmental signals to regulate cell growth and metabolism,mTORC1 is recruited to the lysosome where it is activated by its interaction with GTP-bound Rheb GTPase.However,the regulatory mechanism of Rheb activity remains largely unknown.Here,we show that ubiquitination governs the nucleotide-bound status of Rheb.Lysosome-anchored E3 ligase RNF152 catalyzes Rheb ubiquitination and promotes its binding to the TSC complex.EGF enhances the deubiquitination of Rheb through AKT-dependent USP4 phosphorylation,leading to the release of Rheb from the TSC complex.Functionally,ubiquitination of Rheb is linked to mTORC1-mediated signaling and consequently regulates tumor growth.Thus,we propose a mechanistic model whereby Rheb-mediated mTORC1 activation is dictated by a dynamic opposing act between Rheb ubiquitination and deubiquitination that are catalyzed by RNF152 and USP4 respectively. | Lu Deng Lei Chen Linlin Zhao Yan Xu Xiaoping Peng Xinbo Wang Lin Ding Jiali Jin Hongqi Teng Yanming Wang Weijuan Pan Fei Yu Lujian Liao Li Li Xin Ge Ping Wang | 2019 | Cell Research2019，29，2： | 16 |
| 3 | Decreased osteogenesis of adult mesenchymal stem cel s by reactive oxygen species under cyclic stretch: a possible mechanism of age related osteoporosis显示文摘Age related defect of the osteogenic differentiation of mesenchymal stem cells(MSCs) plays a key role in osteoporosis. Mechanical loading is one of the most important physical stimuli for osteoblast differentiation.Here, we compared the osteogenic potential of MSCs from young and adult rats under three rounds of 2 h of cyclic stretch of 2.5% elongation at 1 Hz on 3 consecutive days. Cyclic stretch induced a significant osteogenic differentiation of MSCs from young rats, while a compromised osteogenesis in MSCs from the adult rats.Accordingly, there were much more reactive oxygen species(ROS) production in adult MSCs under cyclic stretch compared to young MSCs. Moreover, ROS scavenger N-acetylcysteine rescued the osteogenic differentiation of adult MSCs under cyclic stretch. Gene expression analysis revealed that superoxide dismutase 1(SOD1) was significantly downregulated in those MSCs from adult rats. In summary, our data suggest that reduced SOD1 may result in excessive ROS production in adult MSCs under cyclic stretch, and thus manipulation of the MSCs from the adult donors with antioxidant would improve their osteogenic ability. | Jiali Tan Xin Xu Zhongchun Tong Jiong lin Qiujun Yu Yao Lin Wei Kuang | 2015 | Bone Research2015，3，1： | 15 |
| 4 | Dysregulation of the miR-146a-Smad4 axis impairs osteogenesis of bone mesenchymal stem cells under inflammation显示文摘Osteoporosis is a common disease that affects patient quality of life, especially among the elderly population.Although inflammation contributes significantly to osteoporosis, the underlying mechanism is unclear. In this study, we found that tumor necrosis factor(TNF)-α, an inflammatory environment mimic, inhibits osteogenesis of bone mesenchymal stem cells(BMSCs), induces mi R-146 a and decreases Smad4. Moreover,overexpression of mi R-146 a inhibited the osteogenic ability of BMSCs, whereas blocking mi R-146 a partially rescued the osteogenesis deficiency under TNF-α treatment. Molecularly, mi R-146 a decreased Smad4 expression at the protein level by binding to an element located in the Smad4 30-untranslated region, and restoration of Smad4 reversed the inhibitory effects of mi R-146 a on osteogenesis. Together, our results showed that the inflammatory environment mimic TNF-α inhibits osteogenesis via upregulation of mi R-146 a and subsequent downregulation of Smad4, thus suggesting that therapeutic manipulation of mi R-146 a maybe a potential strategy to improve osteogenesis in the context of osteoporosis. | Wei Kuang Liwei Zheng Xin Xu Yao Lin Jiong Lin Jiahua Wu Jiali Tan | 2017 | Bone Research2017，5，4： | 13 |
| 5 | The effect of Scuteilaria baicalensis stem-leaf flavonoids on spatial learning and memory in chronic cerebral ischemia-induced vascular dementia of rats显示文摘Flavonoids 被显示了改进认知功能并且推迟痴呆前进。然而,内在的机制留下逃犯。在现在的学习,我们检验了从 S 提取的黄芩 baicalensis 茎叶总数 flavonoids (SSTF ) 的效果。在脉管的痴呆(VaD ) 的空间学习和记忆上的 baicalensis Georgi 老鼠模型并且探索了它的分子的机制。VaD 老鼠被普通颈动脉动脉的永久双边的吸藏开发。在恢复以后的七天, VaD 老鼠与 SSTF 的 50 或 100 mg/kg 被对待 60 天。空间学习和记忆在莫利斯舞水迷宫(MWM ) 被评估测试。字形物 hyperphosphorylation 和相关蛋白质 kinases 或磷酸酶的层次被西方的污点分析检验。在 VaD 老鼠,在 100 mg/kg 的 SSTF 处理显著地在在 MWM 测试训练试用减少了逃跑潜伏。在探针试用, SSTF 治疗在目标象限增加了寻找的时间和旅行距离。SSTF 处理在 VaD 老鼠在外皮和马头鱼尾的怪兽禁止了字形物 phosphorylation。同时, SSTF 减少了肝糖 synthase kinase 的活动 3 并且 cyclin 依赖的 kinase 5 在 VaD 老鼠。相反, SSTF 处理在 VaD 老鼠增加了蛋白质磷酸酶 2A 子单元 B 的水平。SSTF 处理显著地在 VaD 老鼠改进了空间认知。我们的结果建议 SSTF 可以通过在击以后协调 kinases 和磷酸酶的活动减轻 tau-hyperphosphorylation-induced neurotoxicity。SSTF 可以为 VaD 被开发进有希望的新奇治疗学。 | Yanjing Cao Lizhen Liang Jian Xu Jiali Wu Yongxing Yan Ping Lin Qiang Chen Fengming Zheng Qin Wang Qian Ren Zengmei Gou Yifeng Du | 2016 | Acta Biochimica et Biophysica Sinica2016，48，5： | 10 |
| 6 | Performance Evaluation of Two-Vector-Based Model Predictive Current Control of PMSM Drives显示文摘Conventional model predictive current control(MPCC)applies only one vector during one control period,which produces large torque and flux ripples and high current harmonics in permanent magnet synchronous motor(PMSM)drives.Recently MPCC with duty cycle control has been proposed to improve the steady state performance by applying one non-zero vector and one null vector during one control period.However,the prior method requires lots of calculations and predictions to find the optimal voltage vectors and calculate their respective duration.Different from prior enumeration-based MPCC,this paper proposes an efficient two-vector MPCC by applying two arbitrary voltage vectors during one control period.The reference voltage vector is firstly calculated based on the principle of deadbeat current control.Two optimal vectors and their duration are then obtained in a very efficient way,which does not require the calculation of current slopes in prior MPCC methods.The proposed method is compared to the state-of-the-art predictive control methods,including conventional MPCC,MPCC with duty cycle control,deadbeat control with space vector modulation(SVM)and modulated model predictive control(M2PC).Both simulation and experimental results prove that the proposed method achieves better steady state performance than conventional MPCC with or without duty cycle and the dynamic response is not degraded.Under the condition of insufficient dc bus voltage,the proposed method outperforms deadbeat control and M2PC by presenting even higher speed range and less torque ripples. | Yongchang Zhang Lanlan Huang Donglin Xu Jiali Liu Jialin Jin | 2018 | Chinese Journal of Electrical Engineering2018，4，2： | 9 |
| 7 | Efficacy and safety of low-dose interleukin-2 in combination with methotrexate in patients with active rheumatoid arthritis:a randomized,double-blind,placebo-controlled phase 2 trial显示文摘Rheumatoid arthritis(RA)is an aggressive autoimmune arthritis,and current therapies remain unsatisfactory due to low remission rate and substantially adverse effects.Low-dose interleukin-2(Ld-IL2)is potentially a therapeutic approach to further improve the disease.This randomized,double-blind,placebo-controlled trial was undertaken to evaluate the efficacy and safety of Ld-IL2 in patients with active RA.Patients were randomly assigned(1:1)to receive Ld-IL2,defined as a dose of 1 million IU,or placebo in a 12-week trial with a 12-week follow-up.Three cycles of Ld-IL2 or placebo were administered subcutaneously every other day for 2 weeks(a total of 7 doses),followed by a 2-week break.All patients received a stable dose of methotrexate(MTX).The primary outcomes were the proportion of patients achieving the ACR20,DAS28-ESR<2.6,and the change from baseline in CDAI or SDAI at week 24.Secondary endpoints included other clinical responses and safety.The primary outcomes were achieved in the perprotocol population.The improvements from baseline in CDAI and SDAI were significantly greater across time points for the LdIL2+MTX group(n=17)than for the placebo+MTX group(n=23)(P=0.018 and P=0.015,respectively).More patients achieved ACR20 response in the Ld-IL2+MTX group than those in the placebo+MTX group at week 12(70.6%vs 43.5%)and at week 24(76.5%vs 56.5%)(P=0.014).In addition,low Treg and high IL-21 were associated with good responses to Ld-IL2.Ld-IL-2 treatment was well-tolerated in this study.These results suggested that Ld-IL2 was effective and safe in RA.ClinicalTrials.gov number:NCT 02467504. | Xiaoying Zhang Miao Miao Ruijun Zhang Xu Liu Xiaozhen Zhao Miao Shao Tian Liu Yuebo Jin Jiali Chen Huixin Liu Xia Zhang Yun Li Yunshan Zhou Yue Yang Ru Li Haihong Yao Yanying Liu Chun Li Yuhui Li Limin Ren Yin Su Xiaolin Sun Jing He Zhanguo Li | 2022 | Signal Transduction and Targeted Therapy2022，7，4： | 7 |
| 8 | Novel synthesis of fly-ash-derived Cu-loaded SAPO-34 catalysts and their use in selective catalytic reduction of NO with NH3显示文摘A combined acid–alkali hydrothermal method was used to prepare fly ash–derived SAPO-34 molecular sieves from a thermal power plant in Inner Mongolia(China).The specific surface area of the prepared fly-ash-derived SAPO-34 molecular sieves was 579 m^2 g^-1,the total pore volume was about 0.27 cm^3 g^-1,and the pore size was 0.56 nm;the molar ratios of Al2O3:P2O5:SiO2 were 1:0.86:0.45.Cu/SAPO-34 catalysts were prepared by impregnation of low-cost fly-ash-derived SAPO-34 molecular sieves as a support and tested in selective catalytic reduction with NH3(NH3-SCR).Powder X-ray diffraction(XRD),N2 adsorption–desorption,X-ray photoelectron spectroscopy(XPS),H2 temperatureprogrammed reduction(H2-TPR),NH3 temperature-programmed desorption(NH3-TPD),electron paramagnetic resonance(EPR),nuclear magnetic resonance(NMR),X-ray fluorescence analysis(XRF)and scanning electron microscopy(SEM)were used for catalyst characterization and investigation of the relationships between the catalyst structure and the catalytic activity.The actual silica:alumina ratio of the molecular sieves did not increase with increasing Cu loading,indicating that increasing the Cu loading does not change the original structure of the SAPO-34 molecular sieves.The XRF and NMR results showed that replacement by Cu results in more Si islands.The molecular sieve acidity decreased because of the increased number of Si islands.The NH3-TPD results showed that for the Cu/SAPO-34 catalysts there was a low correlation between the low-temperature activity and the amount of acidic sites.SCR activity is closely related to the location of Cu.The 4.47 Cu/SAPO-34 catalyst has the highest isolated Cu2+showed the highest NH3-SCR activities(>90%)at 250–350℃.This work opens up new avenues for recycling fly ash formed in coal-fired power plants(reducing environmental pollution)and developing low-cost SCR catalysts for NOx pollution control. | Ge Li Baodong Wang Qi Sun Wayne Qiang Xu Ziran Ma Hongyan Wang Daojun Zhang Jiali Zhou | 2019 | Green Energy & Environment2019，4，4： | 6 |
| 9 | OsMKKK70 regulates grain size and leaf angle in rice through the OsMKK4-OsMAPK6-OsWRKY53 signaling pathway显示文摘Grain size and leaf angle are key agronomic traits that determine final yields in rice.However,the underlying molecular mechanisms are not well understood.Here we demonstrate that the Oryza sativa Mitogen Activated Protein Kinase Kinase Kinase OsMKKK70 regulates grain size and leaf angle in rice.Overexpressing OsMKKK70 caused plants to produce longer seeds.The osmkkk62/70 double mutant and the osmkkk55/62/70 triple mutant displayed significantly smaller seeds and a more erect leaf angle compared to the wild type,indicating that OsMKKK70 functions redundantly with its homologs Os MKKK62 and Os MKKK55.Biochemical analysis demonstrated that OsMKKK70 is an active kinase and that OsMKKK70 interacts with Os MKK4 and promotes Os MAPK6 phosphorylation.In addition,the osmkkk62/70 double mutant showed reduced sensitivity to Brassinosteroids(BRs).Finally,overexpressing constitutively active Os MKK4,Os MAPK6,and Os WRKY53 can partially complement the smaller seed size,erect leaf,and BR hyposensitivity of the osmkkk62/70 double mutant.Taken together,these findings suggest that OsMKKK70 might regulate grain size and leaf angle in rice by activating Os MAPK6 and that OsMKKK70,Os MKK4,Os MAPK6,and Os WRKY53 function in a common signaling pathway that controls grain shape and leaf angle. | Zhiqi Liu Enyang Mei Xiaojie Tian Mingliang He Jiaqi Tang Min Xu Jiali Liu Lu Song Xiufeng Li Zhenyu Wang Qingjie Guan Qijiang Xu Qingyun Bu | 2021 | Journal of Integrative Plant Biology2021，63，12： | 6 |
| 10 | Cooperative and Competitive Multi-Agent Systems:From Optimization to Games显示文摘Multi-agent systems can solve scientific issues related to complex systems that are difficult or impossible for a single agent to solve through mutual collaboration and cooperation optimization.In a multi-agent system,agents with a certain degree of autonomy generate complex interactions due to the correlation and coordination,which is manifested as cooperative/competitive behavior.This survey focuses on multi-agent cooperative optimization and cooperative/non-cooperative games.Starting from cooperative optimization,the studies on distributed optimization and federated optimization are summarized.The survey mainly focuses on distributed online optimization and its application in privacy protection,and overviews federated optimization from the perspective of privacy protection me-chanisms.Then,cooperative games and non-cooperative games are introduced to expand the cooperative optimization problems from two aspects of minimizing global costs and minimizing individual costs,respectively.Multi-agent cooperative and non-cooperative behaviors are modeled by games from both static and dynamic aspects,according to whether each player can make decisions based on the information of other players.Finally,future directions for cooperative optimization,cooperative/non-cooperative games,and their applications are discussed. | Jianrui Wang Yitian Hong Jiali Wang Jiapeng Xu Yang Tang Qing-Long Han Jürgen Kurths | 2022 | IEEE/CAA Journal of Automatica Sinica2022，9，5： | 5 |
| 11 | Self-propagating high-temperature synthesis of ZrO_2 incorporated Gd_2Ti_2O_7 pyrochlore显示文摘In this research, Zr-doped Gd_2Ti_2O_7 pyrochlores, with the composition of Gd_2(Ti_(1-x)Zr_x)_2O_7, were firstly synthesized by self-propagating high-temperature synthesis plus quick pressing(SHS/QP) using CuO as the oxidant and Ti as the reductant. To improve the radiation resistance of titanate–pyrochlore, up to 35 at% Zr was incorporated to substitute the Ti site of Gd_2Ti_2O_7 pyrochlore(Gd_2(Ti_(0.75)Zr_(0.35))_2O_7). XRD and SEM microstructural characterizations showed the formation of a composite ceramic with the major pyrochlore phase and the minor Cu phase. The generated temperature of samples decreased from 1702to 1011 ℃ with increasing Zr content. The effects of sintering temperature and pressure time on phase composition and microstructure were systematically studied. Besides, the influence of thermal transmission on the whole combustion process was also explored. The pyrochlore-based waste form possessed high bulk density of 6.25 g/cm^3 and Vickers hardness of 10.81 GPa. The MCC-1 leaching test showed the normalized elemental leaching rates(42d) of Cu, Gd, and Zr are 1.27×10^(-2), 1.33×10^(-3), and 8.44×10^(-7)g·m^(-2)·d^(-1), respectively. | Le PENG Kuibao ZHANG Zongsheng HE Dan YIN Jiali XUE Chen XU Haibin ZHANG | 2018 | Journal of Advanced Ceramics2018，7，1： | 5 |
| 12 | Comparison of Clinical and Epidemiological Characteristics of Asymptomatic and Symptomatic SARS-CoV-2 Infection in Children显示文摘To understand the epidemiological and clinical features of the symptomatic and asymptomatic pediatric cases of COVID-19,we carried out a prospective study in Shanghai during the period of January 19 to April 30,2020.A total of 49 children(mean age 11.5±5.12 years)confirmed with SARS-CoV-2 infection were enrolled in the study,including 11(22.4%)domestic cases and 38(77.6%)imported cases.Nine(81.8%)local cases and 12(31.6%)imported cases had a definitive epidemiological exposure.Twenty-eight(57.1%)were symptomatic and 21(42.9%)were asymptomatic.Neither asymptomatic nor symptomatic cases progressed to severe diseases.The mean duration of viral shedding for SARS-CoV-2 in upper respiratory tract was 14.1±6.4 days in asymptomatic cases and 14.8±8.4 days in symptomatic cases(P>0.05).Forty-five(91.8%)cases had viral RNA detected in stool.The mean duration of viral shedding in stool was 28.1±13.3 days in asymptomatic cases and 30.8±18.6 days in symptomatic participants(P>0.05).Children<7 years shed viral RNA in stool for a longer duration than school-aged children(P<0.05).Forty-three(87.8%)cases had seropositivity for antibodies against SARS-CoV-2 within 1–3 weeks after confirmation with infection.In conclusion,asymptomatic SARS-CoV-2 infection may be common in children in the community during the COVID-19 pandemic wave.Asymptomatic cases shed viral RNA in a similar pattern as symptomatic cases do.It is of particular concern that asymptomatic individuals are potentially seed transmission of SARS-CoV-2 and pose a challenge to disease control. | Jiehao Cai Xiangshi Wang Jun Zhao Yanling Ge Jin Xu He Tian Hailing Chang Aimei Xia Jiali Wang Jinqiang Zhang Zhongqiu Wei Jingjing Li Chuning Wang Jianshe Wang Qirong Zhu Xiaowen Zhai Mei Zeng | 2020 | Virologica Sinica2020，35，6： | 4 |
| 13 | Spirulina platensis aqueous extracts ameliorate colonic mucosal damage and modulate gut microbiota disorder in mice with ulcerative colitis by inhibiting inflammation and oxidative stress显示文摘Ulcerative colitis(UC)is a chronic and recurrent inflammatory bowel disease(IBD)that has become a major gastroenterologic problem during recent decades.Numerous complicating factors are involved in UC development such as oxidative stress,inflammation,and microbiota disorder.These factors exacerbate damage to the intestinal mucosal barrier.Spirulina platensis is a commercial alga with various biological activity that is widely used as a functional ingredient in food and beverage products.However,there have been few studies on the treatment of UC using S.platensis aqueous extracts(SP),and the underlying mechanism of action of SP against UC has not yet been elucidated.Herein,we aimed to investigate the modulatory effect of SP on microbiota disorders in UC mice and clarify the underlying mechanisms by which SP alleviates damage to the intestinal mucosal barrier.Dextran sulfate sodium(DSS)was used to establish a normal human colonic epithelial cell(NCM460)injury model and UC animal model.The mitochondrial membrane potential assay 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)and staining with Annexin V-fluorescein isothiocyanate(FITC)/propidium iodide(PI)and Hoechst 33258 were carried out to determine the effects of SP on the NCM460 cell injury model.Moreover,hematoxylin and eosin(H&E)staining,transmission electron microscopy(TEM),enzyme-linked immunosorbent assay(ELISA),quantitative real-time polymerase chain reaction(qPCR),western blot,and 16S ribosomal DNA(rDNA)sequencing were used to explore the effects and underlying mechanisms of action of SP on UC in C57BL/6 mice.In vitro studies showed that SP alleviated DSS-induced NCM460 cell injury.SP also significantly reduced the excessive generation of intracellular reactive oxygen species(ROS)and prevented mitochondrial membrane potential reduction after DSS challenge.In vivo studies indicated that SP administration could alleviate the severity of DSS-induced colonic mucosal damage compared with the control group.Inhibition of inflammation and oxidative stress was associated with increases in the activity of antioxidant enzymes and the expression of tight junction proteins(TJs)post-SP treatment.SP improved gut microbiota disorder mainly by increasing antioxidant enzyme activity and the expression of TJs in the colon.Our findings demonstrate that the protective effect of SP against UC is based on its inhibition of pro-inflammatory cytokine overproduction,inhibition of DSS-induced ROS production,and enhanced expression of antioxidant enzymes and TJs in the colonic mucosal barrier. | Jian WANG Liqian SU Lun ZHANG Jiali ZENG Qingru CHEN Rui DENG Ziyan WANG Weidong KUANG Xiaobao JIN Shuiqing GUI Yinghua XU Xuemei LU | 2022 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2022，23，6： | 3 |
| 14 | The role of carotenoid isomerase in maintenance of photosynthetic oxygen evolution in rice plant显示文摘类胡萝卜素 isomerase (CRTISO ) 被建议了保护 photosystem II (PS II ) 免受 photodamage 的伤害,可能通过它的产品黄体素。然而, photoprotection 的机制尚待进一步被阐明。在这个工作,我们克隆变异的基因报导了编码为在由一条基于地图的克隆途径的米饭异种 zel1 的黄体素的累积负责的 CRTISO 的一个点。变异的显型被转变与野类型(WT ) 的相应基因救。光合的氧进化的活动显然在 zel1 被压制。PS II CP47 的核心蛋白质的数量比 WT 的在 LHCII-PS II supercomplexes 和 zel1 的 CP43 免费的 PS II 特别在所有 PS II 建筑群是低得多的。在另一方面, zel1 的 PS II CP43 的另一核心蛋白质的数量在更高的 supercomplexes 被减少,而它在更低的和 PS II 单体被增加。immunodetection 显示了那 CP43, CP47,和演变氧的外来的蛋白质 PsbO 和 PsbP 被减少,但是反应中心蛋白质 D1 的数量没在 zel1 显示出重要变化。北污点分析证明 CP43 的 transcriptional 水平是下面调整的然而并非在 zel1 的 CP47 或 D1。另外, plastoquinone (PQ ) 质量保证处于在 zel1 的一个减少的状态。根据结果,我们建议 CRTISO 可能在由影响 PQ 的氧化还原作用状态在米饭植物稳定氧进化建筑群的外来的蛋白质调整 CP43 的抄写和 CP47 的翻译工作。 | Jiali Wei Min Xu Dabing Zhang Hualing Mi | 2010 | Acta Biochimica et Biophysica Sinica2010，42，7： | 3 |
| 15 | Differences between physiological and pathological convulsive thresholds in patients with epilepsy显示文摘BACKGROUND: Physiological convulsive thresholds degrade when the brain is in some pathologic states; thus, a level of stimulus that cannot provoke a convulsion may evoke a seizure or epileptic seizure. OBJECTIVE: To investigate the changes that occur in the brain when the physiological convulsive threshold becomes pathological, and to determine what differences occur in pathological and physiological convulsive thresholds during the development of epilepsy. DESIGN: A randomized controlled animal experiment. SETTING: Research Institute of Epilepsy of Shanxi Medical University; Department of Neurology, The Third Hospital of Shanxi Medical University; Research Institute of Function of Shanxi Medical University. MATERIALS: Thirty-six female Wistar rats were selected for this study. The rats were obtained from the experimental animal center of Shanxi Medical University. All laboratory procedures complied with animal ethical standards. The animals were randomly divided into three groups: a strong current group, a weak current group and a control group, with 12 rats in each group. An automatic determinator of seizure threshold was made at Shanxi Medical University and Taiyuan University of Technology. Two bipolar stainless steel stimulating electrodes and an electrode connector (diameter 1.2 mm) were made at Taiyuan University of Technology. METHODS: This study was performed in the laboratory of Research Institute of the Epilepsy of Shanxi Medical University between December 2005 and August 2006. The threshold of localized seizures was measured by performing direct cortical stimulation in rats under anesthesia. After 1 week of post-operative recovery, electric stimulation was started with three different kinds of stimulation. Seizure activity was induced by a ramp-shaped single train of biphasic pulses (50 Hz, total pulse duration of 2 ms, increasing from 0 to 2 000 μA in 15 seconds). The threshold of localized seizures (TLS) has been defined as the minimum current intensity necessary to provoke convulsion of the forelimbs and/or facial muscles. Up to the TLS, if stimulation continued, the current intensity necessary to provoke the generalized seizures is called the threshold of generalized seizures (TGS). If stimulation is continued for about 2 seconds when the TGS is reached, rats still showed generalized clonic activity after stimulation ceased. When seizures stopped, a short period of immobility can be observed. The current intensity is called the threshold of prolonged seizures (TPS). The rats in the strong current group were stimulated up to the current level required to reach the TPS. In the course of stimulation, first, the TLS was recorded, then the TGS, and finally the TPS. The stimulation interval in one session was 10 minutes, repeated twice daily. The rats in the weak current group were only stimulated up to the current levels required to reach the TGS; first, the TLS was recorded and then the TGS was measured at the same time as the strong current group. Control animals were also equipped with a full electrode set and placed in the same conditions, but no stimulation took place, only electroencephalogram (EEG) recording at the same times as the experimental groups. MAIN OUTCOME MEASURES: ① Stimulation of the two experimental groups lasted for 11 weeks and then observation of their behavior and electroencephalogram recording continued for 4 weeks. The control group was also observed over a total of 15 weeks. ② Observing neuronal damage/loss in the hippocampus with a light microscope using a 250× visual field. RESULTS: All 36 Wistar rats were included in the final analysis. At the beginning of the experiment, the convulsive thresholds were all above 1 100 μA, although there were significant individual variations among rats of the same group. Those thresholds quickly declined during the initial 4 weeks of repetitive electrical stimulation. The convulsive thresholds approached a constant level in the 10th week after commencement of stimulation. There were no significant changes in thresholds when stimulations lasted longer; the convulsive thresholds and the variations in rats of the same group were significantly lower than at the beginning of the trial (P < 0.01). An interictal discharge was also recorded in the 3rd week in the strong current group, and in the 8th week in the weak current group; these discharges were concomitant with neuronal damage and loss in the hippocampus. There was no abnormality observed in the control group. CONCLUSION: These findings indicated that the convulsion threshold in the brain should be divided intotwo stages: a physiological convulsive threshold and a pathological convulsive threshold (epileptic threshold). The epileptic threshold is created by pathologically acquired factors, which give rise to brain damage. The increase in the intensity of these pathologically acquired factors led to aggravation of damage. | Yuxi Liu Leiyu Geng Jiali Xu Mingzheng Wang Ce Zhang Yu Zhang | 2008 | Neural Regeneration Research2008，3，3： | 3 |
| 16 | A genome-wide association study uncovers a critical role of the RsPAP2 gene in red-skinned Raphanus sativus L.显示文摘Radish(Raphanus sativus L.)taproot contains high concentrations of flavonoids,including anthocyanins(ATCs),in redskinned genotypes.However,little information on the genetic regulation of ATC biosynthesis in radish is available.A genome-wide association study of radish red skin color was conducted using whole-genome sequencing data derived from 179 radish genotypes.The R2R3-MYB transcription factor production of anthocyanin pigment 2(PAP2)gene was found in the region associated with a leading SNP located on chromosome 2.The amino acid sequence encoded by the RsPAP2 gene was different from those of the other published RsMYB genes responsible for the red skin color of radish.The overexpression of the RsPAP2 gene resulted in ATC accumulation in Arabidopsis and radish,which was accompanied by the upregulation of several ATC-related structural genes.RsPAP2 was found to bind the RsUFGT and RsTT8 promoters,as shown by a dual-luciferase reporter system and a yeast one-hybrid assay.The promoter activities of the RsANS,RsCHI,RsPAL,and RsUFGT genes could be strongly activated by coinfiltration with RsPAP2 and RsTT8.These findings showed the effectiveness of GWAS in identifying candidate genes in radish and demonstrated that RsPAP2 could(either directly or together with its cofactor RsTT8)regulate the transcript levels of ATC-related genes to promote ATC biosynthesis,facilitating the genetic enhancement of ATC contents and other related traits in radish. | Lianxue Fan YanWang Liang Xu Mingjia Tang Xiaoli Zhang Jiali Ying Cui Li Junhui Dong Liwang Liu | 2020 | Horticulture Research2020，7，1： | 2 |
| 17 | SAR Imaging Algorithm Filter for Based on Nonstationary FLGPR显示文摘 | FAN Yong ZHOU Zhengou XU Jiali | 2008 | Chinese Journal of Electronics2008，17，1： | 2 |
| 18 | NUMB enhances Notch signaling by repressing ubiquitination of NOTCH1 intracellular domain显示文摘The release and nuclear translocation of the intracellular domain of Notch receptor(NICD)is the prerequisite for Notch signaling-mediated transcriptional activation.NICD is subjected to various posttranslational modifications including ubiquitination.Here,we surprisingly found that NUMB proteins stabilize the intracellular domain of NOTCH1 receptor(N1ICD)by regulating the ubiquitin–proteasome machinery,which is independent of NUMB’s role in modulating endocytosis.BAP1,a deubiquitinating enzyme(DUB),was further identified as a positive N1ICD regulator,and NUMB facilitates the association between N1ICD and BAP1 to stabilize N1ICD.Intriguingly,BAP1 stabilizes N1ICD independent of its DUB activity but relying on the BRCA1-inhibiting function.BAP1 strengthens Notch signaling and maintains stem-like properties of cortical neural progenitor cells.Thus,NUMB enhances Notch signaling by regulating the ubiquitinating activity of the BAP1–BRCA1 complex. | Zhiyuan Luo Lili Mu Yue Zheng Wenchen Shen Jiali Li Lichao Xu Bo Zhong Ying Liu Yan Zhou | 2020 | Journal of Molecular Cell Biology2020，12，5： | 2 |
| 19 | Efficacy and tolerance of tacrolimus and pimecrolimus for atopic dermatitis: a meta-analysis显示文摘Tacrolimus ointment and pimecrolimus cream have proved to be suitable for the treatment of atopic dermatitis. We conducted a meta-analysis of the efficacy, adverse events/withdrawal of tacrolimus versus pimecrolimus in the treatment of atopic dermatitis. According to our meta-analysis, 0.1% tacrolimus was more effective than 1% pimecrolimus in the treatment of adult patients and moderate to very severe pediatric patients, and more 0.1% mild pediatric patients treatal with pimecrolimus withdrew from the trials because of a lack of efficacy or the occurrence of adverse events, compared with mild pediatric patients treated with 0.03% tacrolimus. The combined analyses of tacrolimus with pimecrolimus showed that tacrolimus was more effective than pimecrolimus (week 3: RR=0.67, 95% CI=0.56-0.80; week 6/end of study: RR=0.65, 95% CI=0.57-0.75), and fewer tacrolimus-treated patients withdrew because of a lack of efficacy (RR=0.32, 95CI%=0.19-0.53) or the occurrence of adverse events (RR=0.43, 95% CI=0.24-0.75), compared with pimecrolimus-treated patients. In conclusion, tacrolimus has higher efficacy and better tolerance than pimecrolimus in the treatment of atopic dermatitis. | Zhiqiang Yin Jiali Xu Dan Luo | 2011 | The Journal of Biomedical Research2011，25，6： | 1 |
| 20 | A meta-analysis of the effects of statins on serum C-reactive protein in Chinese population with coronary heart disease or hyperlipidemia显示文摘There is little doubt that statins reduce cardiovascular events more than what the lipid lowering effect can account for. Additional mechanisms have been postulated including the anti-inflammatory effects manifested by reduced C-reactive protein (CRP). It is not known, however, whether statins can decrease CRP in Chinese population. The aim of this study is to investigate the effects of statins on serum CRP in Chinese patients with coronary heart disease (CHD) or hyperlipidemia. Trials were retrieved through Medline (1980 to May, 2009), bibliographies, and the author’s reference files limited to English-language articles. Data were extracted and meta-analysis was performed. Analysis showed statistically significant reduction in CRP after statin treatment (weighted mean difference [WMD] = -0.73, 95% confidence interval [CI] = [-0.80, -0.66], P < 0.00001) and lower CRP after statin treatment than non-statin routine treatment ([WMD] = -0.52, 95% CI [-0.86, -0.18], P = 0.002). In conclusion, statins significantly reduce serum CRP in Chinese population, which may contribute to statin-induced reduction in the cardiovascular risk in addition to the lipid lowering effect. | HAO PanPan CHEN YuGuo WANG XingLi XU Feng WANG JiaLi ZHANG Yun | 2009 | Chinese Science Bulletin2009，54，23： | 1 |
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