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| 1 | Influence of interleukin polymorphisms on development of gastric cancer and peptic ulcer显示文摘Pro-inflammatory cytokines are produced in the gastric mucosa by inflammatory cells activated by chronic Helicobacter pylori (H. pylori) infection. Polymorphisms of these cytokine genes are associated with individual differences in gastric mucosal cytokine mRNA level, which result in differences in gastric mucosal inflammation, acid inhibition and gastroduodenal disease risk in response to H. pylori infection. Although polymorphisms of interleukin (IL)-1B, IL-1RN and TNF-A have been reported to relate well with gastric cancer and peptic ulcer risk, those of IL-2, IL-4, IL-6 and IL-8 genes are unclear. In combined analyses using data from previous studies, we found that the risk of gastric non-cardia cancer development was significantly associated with IL-4-168 C allele (OR: 0.81, 95% CI: 0.69-1.00) and IL-4-590 T allele carrier status (0.61, 0.53-0.73), and IL-6-174 G/G genotype (2Pro-inflammatory cytokines are produced in the gastric mucosa by inflammatory cells activated by chronic Helicobacter pylori (H. pylori) infection. Polymorphisms of these cytokine genes are associated with individual differences in gastric mucosal cytokine mRNA level, which result in differences in gastric mucosal inflammation, acid inhibition and gastroduodenal disease risk in response to H. pylori infection. Although polymorphisms of interleukin (IL)-1B, IL-1RN and TNF-A have been reported to relate well with gastric cancer and peptic ulcer risk, those of IL-2, IL-4, IL-6 and IL-8 genes are unclear. In combined analyses using data from previous stud- ies, we found that the risk of gastric non-cardia cancer development was significantly associated with IL-4-168 C allele (OR: 0.81, 95% CI: 0.69-1.00) and IL-4-590 T allele carrier status (0.61, 0.53-0.73), and IL-6-174 G/G genotype (2.02, 1.31-3.10). In peptic ulcer development, IL-2-330 G and IL-4-590 T allele carriers had a significantly decreased risk (0.37, 0.27-0.50 and 0.58, 0.34-0.99, respectively). Moreover, IL-2, IL-4, IL-6 and IL-8 gene genotypes prevalence differs among popula- tions. The inflammatory cytokine gene polymorphisms (e.g. IL-4 -590 and IL-6 -572 for gastric cancer, and IL-4-590, IL-6-572 and IL-8-251 for peptic ulcer) have a more potent influence on development of gastroduo- denal diseases in Western than East Asian populations. These cytokine gene polymorphisms, as well as those of IL-1B, IL-1RN and TNF-A, may be used to identify groups at higher risk of gastric cancer and peptic ulcer, and those suitable for their prevention by H. pylori eradication therapy in Western populations..02, 1.31-3.10). In peptic ulcer development, IL-2-330 G and IL-4-590 T allele carriers had a significantly decreased risk (0.37, 0.27-0.50 and 0.58, 0.34-0.99, respectively). Moreover, IL-2, IL-4, IL-6 and IL-8 gene genotypes prevalence differs among populations. The inflammatory cytokine gene polymorphisms (e.g. IL-4 -590 and IL-6 -572 for gastric cancer, and IL-4-590, IL-6-572 and IL-8-251 for peptic ulcer) have a more potent influence on development of gastroduo-denal diseases in Western than East Asian populations. These cytokine gene polymorphisms, as well as those of IL-1B, IL-1RN and TNF-A, may be used to identify groups at higher risk of gastric cancer and peptic ulcer, and those suitable for their prevention by H. pylori eradication therapy in Western populations. | Mitsushige Sugimoto Yoshio Yamaoka Takahisa Furuta | 2010 | World Journal of Gastroenterology2010，16，10： | 60 |
| 2 | Plant ABC Transporters Enable Many Unique Aspects of a Terrestrial Plant's Lifestyle显示文摘陆上的植物有更多的 ATP 有约束力的盒子(ABC ) 二~四次 transporter 基因比另外的有机体,包括他们的祖先的 microalgae。最近的研究发现在这些 transporters 怀有变化的植物展出戏剧的显型,许多哪个与发展过程和为干燥土地上的生活必要的功能有关。这些结果建议在进化期间乘并且假定允许植物适应陆上的环境条件的新奇功能的那 ABC transporters。从这个观点在植物 ABC transporters 上检验文学导致了我们建议多样的 ABC transporters 启用了陆上的植物的生活方式的许多唯一、必要的方面,由搬运越过植物的特定的膜的各种各样的混合物。 | Jae-Ung Hwang Won-Yong Song Daewoong Hong Donghwi Ko Yasuyo Yamaoka Sunghoon Jang Sojeong Yim Eunjung Lee Deepa Khare Kyungyoon Kim Michael Palmgren Hwan Su Yoon Enrico Martinoia Youngsook Lee | 2016 | Molecular Plant2016，9，3： | 20 |
| 3 | Transcriptional silencing of Dickkopf gene family by CpG island hypermethylation in human gastrointestinal cancer显示文摘AIM: To clarify alterations of Dickkopfs (Dkks) and Kremen2 (Krm2) in gastrointestinal cancer. METHODS: We investigated the expression profiles and epigenetic alterations of Dkks and Krm2 genes in gastrointestinal cancer using RT-PCR, tissue microarray analysis, and methylation specific PCR (MSP). Cancer cells were treated with the demethylating agent and/or histone deacetylase inhibitor. WST-8 assays and in vitro invasion assays after treatment with specific siRNA for those genes were performed. RESULTS: Dkks and Krm2 expression levels were reduced in a certain subset of the gastrointestinal cancer cell lines and cancer tissues. This was correlated with promoter hypermethylation. There were significant correlations between Dkks over-expression levels and beta-catenin over-expression in colorectal cancer. In colorectal cancers with beta-catenin over-expression, Dkk-1 expression levels were significantly lower in those with lymph node metastases than in those without. Down-regulation of Dkks expression by siRNA resulted in a significant increase in cancer cell growth and invasiveness in vitro.CONCLUSION: Down-regulation of the Dkks associated to promoter hypermethylation appears to be frequently involved in gastrointestinal tumorigenesis. | Tadateru Maehata Hiroaki Taniguchi Hiroyuki Yamamoto Katsuhiko Nosho Yasushi Adachi Nobuki Miyamoto Chie Miyamoto Noriyuki Akutsu Satoshi Yamaoka Fumio Itoh | 2008 | World Journal of Gastroenterology2008，14，17： | 19 |
| 4 | Roles of Helicobacter pylori BabA in gastroduodenal pathogenesis显示文摘Interactions between BabA and Lewis b (Leb) related antigens are the best characterized adhesin-receptor interactions in Helicobacter pylori (H pylori). Several mechanisms for the regulation of BabA expression are predicted, including at both transcriptional and translational levels. The formation of chimeric proteins (babA /B or babB /A chimeras) seems to play an especially important role in translational regulation. Chimeric BabB/A protein had the potential to bind Leb; however, protein production was subject to phase variation through slipped strand mispairing. The babA gene was cloned initially from strain CCUG17875, which contains a silent babA1 gene and an expressed babA2 gene. The sequence of these two genes differs only by the presence of a 10 bp deletion in the signal peptide sequence of babA1 that eliminates its translational initiation codon. However, the babA1 type deletion was found only in strain CCUG17875. A few studies evaluated BabA status by immunoblot and confirmed that BabA-positive status in Western strains was closely associated with severe clinical outcomes. BabA-positive status also was associated with the presence of other virulence factors (e.g. cagA-positive status and vacA s1 genotype). A small class of strains produced low levels of the BabA protein and lacked Leb binding activity. These were more likely to be associated with increased mucosal inflammation and severe clinical outcomes than BabA-positive strains that exhibited Leb binding activity. The underlying mechanism is unclear, and further studies will be necessary to investigate how the complex BabA-receptor network is functionally coordinated during the interaction of H pylori with the gastric mucosa. | Yoshio Yamaoka | 2008 | World Journal of Gastroenterology2008，14，27： | 14 |
| 5 | Chemoprevention of gastric cancer development after Helicobacter pylori eradication therapy in an East Asian population:Meta-analysis显示文摘BACKGROUND Helicobacter pylori(H.pylori)infection is a risk factor for gastric cancer(GC),especially in East Asian populations.Most East Asian populations infected with H.pylori are at higher risk for GC than H.pylori-positive European and United States populations.H.pylori eradication therapy reduces gastric cancer risk in patients after endoscopic and operative resection for GC,as well as in non-GC patients with atrophic gastritis.AIM To clarify the chemopreventive effects of H.pylori eradication therapy in an East Asian population with a high incidence of GC.METHODS PubMed and the Cochrane library were searched for randomized control trials(RCTs)and cohort studies published in English up to March 2019.Subgroup analyses were conducted with regard to study designs(i.e.,RCTs or cohort studies),country where the study was conducted(i.e.,Japan,China,and South Korea),and observation periods(i.e.,≤5 years and>5 years).The heterogeneity and publication bias were also measured.RESULTS For non-GC patients with atrophic gastritis and patients after resection for GC,4 and 4 RCTs and 12 and 18 cohort studies were included,respectively.In RCTs,the median incidence of GC for the untreated control groups and the treatment groups was 272.7(180.4–322.4)and 162.3(72.5–588.2)per 100000 person-years in non-GC cases with atrophic gastritis and 1790.7(406.5–2941.2)and 1126.2(678.7–1223.1)per 100000 person-years in cases of after resection for GC.Compared with non-treated H.pylori-positive controls,the eradication groups had a significantly reduced risk of GC,with a relative risk of 0.67[95%confidence interval(CI):0.47–0.96]for non-GC patients with atrophic gastritis and 0.51(0.36–0.73)for patients after resection for GC in the RCTs,and 0.39(0.30–0.51)for patients with gastritis and 0.54(0.44–0.67)for patients after resection in cohort studies.CONCLUSION In the East Asian population with a high risk of GC,H.pylori eradication effectively reduced the risk of GC,irrespective of past history of previous cancer. | Mitsushige Sugimoto Masaki Murata Yoshio Yamaoka | 2020 | World Journal of Gastroenterology2020，26，15： | 11 |
| 6 | Prevalence of Helicobacter pylori infection and atrophic gastritis in patients with dyspeptic symptoms in Myanmar显示文摘AIM: To survey the detailed analyses for Helicobacter pylori(H. pylori) infection and gastric mucosal status in Myanmar.METHODS: A total of 252 volunteers with dyspeptic symptoms(155 female and 97 male; mean age of 43.6 ± 14.2 years) was participated in Yangon and Mandalay. The status of H. pylori infection was determined based on 5 different tests including rapid urease test, culture, histology, immunohistochemistry and serology. Histological scores were evaluated according to the update Sydney system and the Operative Link for Gastritis Assessment system. Pepsinogen(PG)Ⅰand PG Ⅱ were measured using enzyme-linked immunosorbent assays.RESULTS: The overall prevalence of H. pylori infectionwas 48.0%. There was no relationship between age and infection rate. Even in young group(less than 29 years old), the H. pylori infection rate was relatively high(41.9%). The prevalence of H. pylori infection was significantly higher in Yangon than that of Mandalay. H. pylori infection was significantly associated with the presence of gastric mucosal atrophy. All 7 subjects with peptic ulcer were infected with H. pylori. Although H. pylori-positive subjects showed stronger gastritis than H. pylori-negative subjects, most cases had mild gastritis.CONCLUSION: We revealed the prevalence of H. pylori infection in patients with dyspeptic symptoms in Myanmar. The H. pylori infection was a risk factor for peptic ulcer and stronger gastritis. | Thein Myint Seiji Shiota Ratha-korn Vilaichone New Ni Than Than Aye Miyuki Matsuda Trang Thi Huyen Tran Tomohisa Uchida Varocha Mahachai Yoshio Yamaoka | 2015 | World Journal of Gastroenterology2015，21，2： | 10 |
| 7 | Helicobacter pylori Bab A in adaptation for gastric colonization显示文摘Helicobacter pylori(H. pylori) as a causative agent of gastric complications, is well adapted for the colonization of gastric mucosa. Although the infectious process depends on several factors, the adhesion to the gastric mucosa is the first and important step. Among several outer membrane proteins, Bab A is one of the significant protein involving in many inflammatory processes in addition to its role in the attachment for the persistent colonization. We performed a Pub Med search using the key words: 'bab A ', 'pylori', 'gastric complications', 'homologous recombination', 'slipped strand mispairing'; a total of 249 articles were displayed. Of these we mainly focused on articles with the full text in English and published between 2005 and 2016. H. pylori Bab A is involved in binding with receptors; however, its synthesis is regulated by phase variation. In this review we confirm that H. pylori bab A can be modulated at the molecular and functional levels to adapt to the stress within the gastro-intestinal tract. | Shamshul Ansari Yoshio Yamaoka | 2017 | World Journal of Gastroenterology2017，23，23： | 8 |
| 8 | Extremely high prevalence of Helicobacter pylori infection in Bhutan显示文摘AIM: To revealed the prevalence of Helicobacter pylori (H. pylori ) infection in the Bhutanese population. METHODS: We recruited a total of 372 volunteers (214 females and 158 males; mean age of 39.6 ± 14.9 years) from three Bhutanese cities (Thimphu, Punaka, and Wangdue). The status of H. pylori infection was determined based on five different tests: the rapid urease test (CLO test), culture, histology, immunohistochemistry (IHC), and serum anti H. pylori -antibody. RESULTS: The serological test showed a significantly higher positive rate compared with the CLO test, culture, histology and IHC (P < 0.001, P < 0.001, P=0.01, and P=0.01, respectively). When the subjects were considered to be H. pylori positive in the case of at least one test showing a positive result, the overall prevalence of H. pylori infection in Bhutan was 73.4%. The prevalence of H. pylori infection significantly decreased with age (P < 0.01). The prevalence of H. pylori infection was lower in Thimphu than in Punakha and Wangdue (P=0.001 and 0.06, respectively). The prevalence of H. pylori infection was significantly higher in patients with peptic ulcers than in those with gastritis (91.4% vs 71.3%, P=0.003). CONCLUSION: The high incidence of gastric cancer in Bhutan may be attributed to the high prevalence of H. pylori infection. | Ratha-korn Vilaichone Varocha Mahachai Seiji Shiota Tomohisa Uchida Thawee Ratanachu-ek Lotay Tshering Nguyen Lam Tung Toshio Fujioka Masatsugu Moriyama Yoshio Yamaoka | 2013 | World Journal of Gastroenterology2013，19，18： | 8 |
| 9 | Antibiotics resistance rate of Helicobacter pylori in Bhutan显示文摘AIM:To survey the antibiotic resistance pattern of Helicobacter pylori(H.pylori)strains isolated from Bhutanese population.METHODS:We isolated 111 H.pylori strains from the gastric mucosa of H.pylori-infected patients in Bhutan in 2010.The Epsilometer test was used to determine the minimum inhibitory concentrations(MICs)of amoxicillin(AMX),clarithromycin(CLR),metronidazole(MNZ),levofloxacin(LVX),ciprofloxacin(CIP),and tetracycline(TET).RESULTS:Nineteen of the isolated H.pylori strains were susceptible to all antibiotics tested.The isolated strains showed the highest rate of antibiotic resistance to MNZ(92/111,82.9%).Among the 92 MNZresistant strains,74 strains(80.4%)showed high-level resistance(MIC≥256 g/mL).Three strains were resistance to LVX(2.7%).These strains were also resistance to CIP.None of the strains showed resistance to CLR,AMX and TET.CONCLUSION:CLR-based triple therapy is a more effective treatment approach over MNZ-based triple therapy for H.pylori infection in Bhutan. | Ratha-korn Vilaichone Yoshio Yamaoka Seiji Shiota Thawee Ratanachu-ek Lotay Tshering Tomohisa Uchida Toshio Fujioka Varocha Mahachai | 2013 | World Journal of Gastroenterology2013，19，33： | 6 |
| 10 | Heat shock protein 72 normothermic ischemia,and the impact of congested portal blood reperfusion on rat liver显示文摘INTRODUCTIONFrom the technical aspect of liver surgery ,control of bleeding during hepatic parenchymal resection is one of the most important procedures in hepatectomy .Pringle,s maneuver ,a temporary cross-clamping of the hepatoduodnal ligament ,has often been used for this purpose[1],This is the simplest and userul technique to reduce intraoperative blood loss . | Chao Liu Dai~1 Zhen Long Xia~1 Makoto Kume~2 Yuzo Yamamoto~2 Kazuhiko Yamagami~2 Nobuhiro Ozaki~2 Yoshio Yamaoka~2 ~1Department of Surgery,The Second Clinical College of China Medical University,Shenyang 110003,Liaoning Province,China ~2Department of Gastroenterological Surgery,Kyoto University Graduate School of Medicine,Kyoto,Japan | 2001 | World Journal of Gastroenterology2001，7，3： | 6 |
| 11 | Prospective Cohort Study of Transarterial Chemoembolization for Unresectable Hepatocellular Carcinoma in 8510 Patients显示文摘 | Kenichi Takayasu Shigeki Arii Iwao Ikai Masao Omata Kiwamu Okita Takafumi Ichida Yutaka Matsuyama Yasuni Nakanuma Masamichi Kojiro Masatoshi Makuuchi Yoshio Yamaoka | 2006 | Gastroenterology2006，，2： | 5 |
| 12 | Long-term survival of a case with multiple liver metastases from duodenal gastrointestinal stromal tumor drastically reduced by the treatment with imatinib and hepatectomy显示文摘工具包受体酷氨酸激酶的组成的激活是在胃肠的基质肿瘤(大意) 的致病的一个关键因素。但是几乎没有 imatinib mesylate (IM ) 和外科疗法的联合是否能与 unresectable 在情况中延长幸存的很少信息多重肝转移。我们报导酷氨酸激酶禁止者 IM 和外科疗法对待的大意的手术后的复发的一个案例。对处理的起始的完全的反应(CR ) 为 18 瞬间继续,但是单个肝转移在 IM 处理期间在左肝的脑叶显示出生长。在再发性瘤的部分切除术以后,手术后的路线是平静的,没有复发,病人为 24 瞬间幸存。当前, imatinib 是为 non-resectable 大意的首要的治疗,但是单个代理人治疗经常导致肿瘤抵抗。就算到 imatinib 的忍耐发生, imatinib 和外科疗法的联合能在这里报导了的一些情况中延长幸存。然而,周期性的大意的很多案例上的进一步的研究是必要的评估治疗与外科相结合的 IM 的有效性。 | Chouhei Sakakura Akeo Hagiwara Koji Soga Koji Miyagawa Susumu Nakashima Tetsuji Yoshikawa Shuichi Kin Yuenn Nakase Nobuki Yamaoka Yoshihiko Sagara Hisakazu Yamagishi | 2006 | World Journal of Gastroenterology2006，12，17： | 5 |
| 13 | Diversity of Helicobacter pylori genotypes in Iranian patients with different gastroduodenal disorders显示文摘AIM:To investigate the diversity of Helicobacter pylori(H.pylori)genotypes and correlations with disease outcomes in an Iranian population with different gastroduodenal disorders.METHODS:Isolates of H.pylori from patients with different gastroduodenal disorders were analyzed after culture and identification by phenotypic and genotypic methods.Genomic DNA was extracted with the QIAamp DNA mini kit(Qiagen,Germany).After DNA extraction,genotyping was done for cagA,vacA(s and m regions),iceA(iceA1,iceA2)and babA with specific primers for each allele using polymerase chain reaction(PCR).All patients’pathologic and clinical data and their relation with known genotypes were analyzed by using SPSS version 19.0 software.2test and Fisher’s exact test were used to assess relationships between categorical variables.The level of statistical significance was set at P<0.05.RESULTS:A total of 71 isolates from 177 patients with different gastroduodenal disorders were obtained.Based on analysis of the cagA gene(positive or negative),vacA s-region(s1or s2),vacA m-region(m1or m2),iceA allelic type(iceA1and iceA2)and babA gene(positive or negative),twenty different genotypic combinations were recognized.The prevalence of cagA,vacA s1,vacA s2,vacA m1,vacA m2,iceA1,iceA2,iceA1+iceA2and babA were 62%,78.9%,19.7%,21.1%,78.9%,15.5%,22.5%,40.8%and 95.8%,respectively.Interestingly,evaluation of PCR results for cagA in 6 patients showed simultaneous existence of cagA variants according to their size diversities that proposed mixed infection in these patients.The most prevalent genotype in cagA-positive isolates was cagA+/vacAs1m2/iceA1+A2/babA+and in cagA-negative isolates was cagA-/vacAs1m2/iceA-/babA+.There were no relationships between the studied genes and histo-pathological findings(H.pylori density,neutrophil activity,lymphoid aggregation in lamina propria and glandular atrophy).The strains which carry cagA,vacAs1/m1,iceA2and babA genes showed significant associations with severe active chronic gastritis(P=0.011,0.025,0.020 and 0.031,respectively).The vacAs1genotype had significant correlation with the presence of the cagA gene(P=0.013).Also,babA genotype showed associations with cagA(P=0.024).In the combined genotypes,only cagA+/vacAs1m1/iceA2/babA+genotype showed correlation with severe active chronic gastritis(P=0.025).CONCLUSION:This genotyping panel can be a useful tool for detection of virulent H.pylori isolates and can provide valuable guidance for prediction of the clinical outcomes. | Farzam Vaziri Shahin Najar Peerayeh Masoud Alebouyeh Tabassom Mirzaei Yoshio Yamaoka Mahsa Molaei Nader Maghsoudi Mohammad Reza Zali | 2013 | World Journal of Gastroenterology2013，19，34： | 5 |
| 14 | 临床活体肝部分移植的适应证及手术时机——附1O例报告显示文摘本文报告10例临床活体肝部分移植术,9例为先天性胆道闭锁症,1例为暴发性肝炎。全组病例均为儿童,年龄:3个月~12岁,其中男3例,女7例。作者指出,现阶段临床活体肝部分移植的适应证多为先天性胆道闭锁症,移植对象多为儿童,手术时机应遵循不同适应证各自的发展规律,采取个体化的原则。但一旦确定了肝移植的方针后,则应尽早手术。 | 管文贤 李开宗 窦科峰 Tanaka Koichi Yamaoka Yoshio | 1997 | 中国微创外科杂志1997，，2： | 4 |
| 15 | Geographical distribution of the incidence of gastric cancer in Bhutan显示文摘AIM: To estimate the prevalence of gastric cancer(GC) in a cohort of patients diagnosed with GC and to compare it with patients diagnosed with all other types of gastro-intestinal(GI) cancer during the same period.METHODS: Between 2008 and 2013,five-year period,the medical records of all GI cancer patients who underwent medical care and confirm diagnosis of cancer were reviewed at the National Referral Hospital,Thimphu which is the only hospital in the country where surgical and cancer diagnosis can be made. Demographic information,type of cancer,and the year of diagnosis were collected.RESULTS: There were a total of 767 GI related cancer records reviewed during the study period of which 354(46%) patients were diagnosed with GC. There were 413 patients with other GI cancer including; esophagus,colon,liver,rectum,pancreas,gall bladder,cholangiocarcinoma and other GI tract cancers. The GC incidence rate is approximately 0.9/10000 per year(367 cases/5years per 800000 people). The geographic distribution of GC was the lowest in the south region of Bhutan 0.3/10000 per year compared to the central region 1.4/10000 per year,Eastern region 1.2/10000 per year,and the Western region 1.1/10000 per year. Moreover,GC in the South part was significantly lower than the other GI cancer in the same region(8% vs 15%; OR = 1.8,95%CI: 1.3-3.1,P = 0.05). Among GC patients,38% were under the age of 60 years,mean age at diagnosis was 62.3(± 12.1) years with male-to-female ratio 1:0.5. The mean age among patients with all other type GI cancer was 60 years(± 13.2) and male-tofemale ratio of 1:0.7. At time of diagnosis of GC,342(93%) were at stage 3 and 4 of and by the year 2013; 80(23%) GC patients died compared to 31% death among patients with the all other GI cancer(P = 0.08).CONCLUSION: The incidence rate of GC in Bhutan is twice as high in the United States but is likely an underestimate rate because of unreported and undiagnosed cases in the villages. The high incidence of GC in Bhutan could be attributed to the high prevalence of Helicobacter pylori infection that we previously reported. The lowest incidence of GC in Southern part of the country could be due to the difference in the ethnicity as most of its population is of Indian and Nepal origin. Our current study emphasizes on the importance for developing surveillance and prevention strategies for GC in Bhutan. | Tashi Dendup James M Richter Yoshio Yamaoka Kinley Wangchuk Hoda M Malaty | 2015 | World Journal of Gastroenterology2015，21，38： | 4 |
| 16 | Genetic and epigenetic characteristics of gastric cancers with JC virus T-antigen显示文摘AIM:To clarify the significance of JC virus(JCV)T-antigen (T-Ag)expression in human gastric cancer. METHODS:We investigated the relationship between T-Ag detected by immunohistochemistry and Epstein- Barr virus(EBV)infection,microsatellite instability (MSI),and genetic and epigenetic alterations in gastric cancers.Mutations in the p53,β-catenin,KRAS,BRAF, PIK3CA genes were analyzed by polymerase chain reaction(PCR)-single strand conformation polymorphism and DNA sequencing.Allelic losses were determined by PCR at 7 microsatellite loci.Aberrant DNA methylation was analyzed by MethyLight assay. RESULTS:JCV T-Ag protein expression was found in 49%of 90 gastric cancer tissues.T-Ag positivity was not correlated with clinicopathological characteristics. T-Ag expression was detected in a similar percentage of EBV positive cancers(4 of 9,44%)and EBV negativecancers(35 of 73,48%).T-Ag expression was detected in a significantly lower percentage of MSI-H cancers (14%)than in non MSI-H cancers(55%,P=0.005). T-Ag expression was detected in a significantly higher percentage of cancers with nuclear/cytoplasmic localization of catenin(15 of 21,71%)than in cancers without(42%,P=0.018).p53 mutations were detected in a significantly lower percentage of T-Ag positive cancers(32%)than in T-Ag negative cancers(57%,P= 0.018).T-Ag positive gastric cancers showed a significant increase in the allelic losses and aberrant methylation compared with T-Ag negative gastric cancers(P=0.008 and P=0.003). CONCLUSION:The results suggest that JCV T-Ag is involved in gastric carcinogenesis through multiple mechanisms of genetic and epigenetic alterations. | Satoshi Yamaoka Hiroyuki Yamamoto Katsuhiko Nosho Hiroaki Taniguchi Yasushi Adachi Shigeru Sasaki Yoshiaki Arimura Kohzoh Imai Yasuhisa Shinomura | 2009 | World Journal of Gastroenterology2009，15，44： | 3 |
| 17 | Epstein–Barr virus-associated hemophagocytic syndrome in a patient with ulcerative colitis during treatment with azathioprine: A case report and review of literature显示文摘A 19-year-old female was diagnosed with ulcerative colitis when she presented with persistent melena, and has been treated with 5-aminosalicylic acid for 4 years,with additional azathioprine for 2 years at our hospital.The patient experienced high-grade fevers, chills, and cough five d prior to presenting to the outpatient unit.At first, the patient was suspected to have developed neutropenic fever; however, she was diagnosed with Epstein-Barr virus-associated hemophagocytic syndrome(EB-VAHS) upon fulfilling the diagnostic criteria after bone marrow aspiration. When patients withinflammatory bowel disease treated with immunomodulators, such as thiopurine preparations, develop fever, EB-VAHS should be considered in the differential diagnosis. | Kazuya Miyaguchi Minoru Yamaoka Yoshikazu Tsuzuki Keigo Ashitani Hideki Ohgo Yoshitaka Miyagawa Keisuke Ishizawa Hidekazu Kayano Hidetomo Nakamoto Hiroyuki Imaeda | 2018 | World Journal of Clinical Cases2018，6，14： | 3 |
| 18 | The significance of virulence factors in H elicobacter pylori显示文摘 | Seiji Shiota Rumiko Suzuki Yoshio Yamaoka | 2013 | Journal of Digestive Diseases2013，，7： | 3 |
| 19 | Pathogenesis of Helicobacter pylori -Related Gastroduodenal Diseases from Molecular Epidemiological Studies显示文摘 | Yoshio Yamaoka Ping-I Hsu | 2012 | Gastroenterology Research and Practice2012，，： | 3 |
| 20 | Synthesis of diamond with the highest nitrogen concentration显示文摘 | Hisao Kanda Minoru Akaishi Shinobu Yamaoka | 1999 | Diamond & Related Materials1999，，： | 2 |
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