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| 1 | The Performance of Whole Genome Amplification Methods and Next-Generation Sequencing for Pre-Implantation Genetic Diagnosis of Chromosomal Abnormalities显示文摘Reliable and accurate pre-implantation genetic diagnosis(PGD) of patient's embryos by next-generation sequencing(NGS) is dependent on efficient whole genome amplification(WGA) of a representative biopsy sample. However, the performance of the current state of the art WGA methods has not been evaluated for sequencing. Using low template DNA(15 pg) and single cells, we showed that the two PCR-based WGA systems Sure Plex and MALBAC are superior to the REPLI-g WGA multiple displacement amplification(MDA) system in terms of consistent and reproducible genome coverage and sequence bias across the 24 chromosomes, allowing better normalization of test to reference sequencing data. When copy number variation sequencing(CNV-Seq) was applied to single cell WGA products derived by either Sure Plex or MALBAC amplification, we showed that known disease CNVs in the range of 3e15 Mb could be reliably and accurately detected at the correct genomic positions. These findings indicate that our CNV-Seq pipeline incorporating either Sure Plex or MALBAC as the key initial WGA step is a powerful methodology for clinical PGD to identify euploid embryos in a patient's cohort for uterine transplantation. | Na Li Li Wang Hui Wang Minyue Ma Xiaohong Wang Yi Li Wenke Zhang Jianguang Zhang David S.Cram Yuanqing Yao | 2015 | Journal of Genetics and Genomics2015，42，4： | 13 |
| 2 | A PGD Pregnancy Achieved by Embryo Copy Number Variation Sequencing with Confirmation by Non-Invasive Prenatal Diagnosis显示文摘Aneuploidy rates in embryos produced by assisted reproductive technologies commonly exceed 50%,particularly in couples where the woman is of advanced maternal age or has experienced repeated implantation failure(Harper et al.,2012).Pre-implantation genetic diagnosis(PGD)conducted at many fertility clinics worldwide is now | Hui Wang Li Wang Minyue Ma Zhuo Song Jianguang Zhang Genming Xu Junmei Fan Na Li David S.Cram Yuanqing Yao | 2014 | Journal of Genetics and Genomics2014，41，8： | 3 |
| 3 | All-optical clock division with mode-locked figure-eight laser based on the slow carrier recovery rate in semiconductor optical amplifier显示文摘 | Lei Xu Minyu Yao Bing C Wang | 2002 | IEEE Photonics Technology Letters2002，14，3： | 1 |
| 4 | Theoretical analyses on short-term stability of semiconductor fiber ring lasers显示文摘 | Qianfang Xu Minyu Yao | 2003 | IEEE J Quantum Electron2003，39，10： | 1 |
| 5 | Alloptical analog-to-digital conversion using inherent multiwavelength phase shift in LiNbO3 phase modulator 显示文摘 | WU Qingwei YAO Minyu ZHANG Hongming | 2008 | IEEE Photonics Technology Letters2008，20，12： | 1 |
| 6 | All-optical clock division with mode-locked figure-eight laser based on the slow carrier recovery rate in semiconductor optical amplifier 显示文摘 | Lei X u Minyu Yao | 2002 | IEEE photonics Techonol Lett2002，14，： | 1 |
| 7 | All-optical queue buffer using optical threshold functions and wavelength converters 显示文摘 | Yuancheng Zhang Hongming Zhang Minyu Yao | 2012 | Chin Opt Lett2012，10，03： | 1 |
| 8 | Single-cell Sequencing Reveals Clearance of Blastula Chromosomal Mosaicism in In Vitro Fertilization Babies显示文摘Although chromosomal mosaic embryos detected by trophectoderm(TE)biopsy offer healthy embryos available for transfer,high-resolution postnatal karyotyping and chromosome testing of the transferred embryos are insufficient.Here,we applied single-cell multi-omics sequencing for seven infants with blastula chromosomal mosaicism detected by TE biopsy.The chromosome ploidy was examined by single-cell genome analysis,with the cellular identity being identified by single-cell transcriptome analysis.A total of 1616 peripheral leukocytes from seven infants with embryonic chromosomal mosaicism and three control ones with euploid TE biopsy were analyzed.A small number of blood cells showed copy number alterations(CNAs)on seemingly random locations at a frequency of 0%-2.5%per infant.However,none of the cells showed CNAs that were the same as those of the corresponding TE biopsies.The blastula chromosomal mosaicism may be fully self-corrected,probably through the selective loss of the aneuploid cells during development,and the transferred embryos can be born as euploid infants without mosaic CNAs corresponding to the TE biopsies.The results provide a new reference for the evaluations of transferring chromosomal mosaic embryos in certain situations. | Yuan Gao Jinning Zhang Zhenyu Liu Shuyue Qi Xinmeng Guo Hui Wang Yanfei Cheng Shuang Tian Minyue Ma Hongmei Peng Lu Wen Fuchou Tang Yuanqing Yao | 2022 | Genomics, Proteomics & Bioinformatics2022，20，6： | 0 |
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