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| 1 | Near-infrared photoimmunotherapy of pancreatic cancer using an indocyanine green-labeled anti-tissue factor antibody显示文摘AIM To investigate near-infrared photoimmunotherapeutic effect mediated by an anti-tissue factor(TF) antibody conjugated to indocyanine green(ICG) in a pancreatic cancer model.METHODS Near-infrared photoimmunotherapy(NIR-PIT) is a highly selective tumor treatment that utilizes an antibody-photosensitizer conjugate administration, followed by NIR light exposure. Anti-TF antibody 1849-ICG conjugate was synthesized by labeling of rat IgG2 b anti-TF monoclonal antibody 1849(anti-TF 1849) to a NIR photosensitizer,ICG. The expression levels of TF in two human pancreatic cancer cell lines were examined by western blotting. Specific binding of the 1849-ICG to TF-expressing BxPC-3 cells was examined by fluorescence microscopy. NIR-PITinduced cell death was determined by cell viability imaging assay. In vivo longitudinal fluorescence imaging was used to explore the accumulation of 1849-ICG conjugate in xenograft tumors. To examine the effect of NIRPIT, tumor-bearing mice were separated into 5 groups:(1) 100 μg of 1849-ICG i.v. administration followed by NIR light exposure(50 J/cm2) on two consecutive days(Days 1 and 2);(2) NIR light exposure(50 J/cm2) only on two consecutive days(Days 1 and 2);(3) 100 μg of 1849-ICG i.v. administration;(4) 100 μg of unlabeled antiTF 1849 i.v. administration; and(5) the untreated control. Semiweekly tumor volume measurements, accompanied with histological and immunohistochemical(IHC) analyses of tumors, were performed 3 d after the 2nd irradiation with NIR light to monitor the effect of treatments. RESULTS High TF expression in BxPC-3 cells was observed via western blot analysis, concordant with the observed preferential binding with intracellular localization of 1849-ICG via fluorescence microscopy. NIR-PIT-induced cell death was observed by performing cell viability imaging assay. In contrast to the other test groups, tumor growth was significantly inhibited by NIR-PIT with a statistically significant difference in relative tumor volumes for 27 d after the treatment start date [2.83 ± 0.38(NIR-PIT) vs 5.42 ± 1.61(Untreated), vs 4.90 ± 0.87(NIR), vs 4.28 ±1.87(1849-ICG), vs 4.35 ± 1.42(anti-TF 1849), at Day 27, P < 0.05]. Tumors that received NIR-PIT showed evidence of necrotic cell death-associated features upon hematoxylin-eosin staining accompanied by a decrease in Ki-67-positive cells(a cell proliferation marker) by IHC examination.CONCLUSION The TF-targeted NIR-PIT with the 1849-ICG conjugate can potentially open a new platform for treatment of TF-expressing pancreatic cancer. | Winn Aung Atsushi B Tsuji Aya Sugyo Hiroki Takashima Masahiro Yasunaga Yasuhiro Matsumura Tatsuya Higashi | 2018 | World Journal of Gastroenterology2018，24，48： | 7 |
| 2 | Combined Administration of L-Cystine and L-Theanine Enhances Immol/Lune Functions and Protects against Influenza Virus Infection in Aged Mice显示文摘 | YASUHIRO TAKAGI SHIGEKAZU KURIHARA NATSUMI HIGASHI | 2010 | Journal of Veterinary Medical Science2010，72，2： | 1 |
| 3 | Efp as a primary estrogen-responsive gene in human breast cancer显示文摘 | Kazuhiro Ikeda Akira Orimo Yasuhiro Higashi Masami Muramatsu Satoshi Inoue | 2000 | FEBS Letters2000，，： | 1 |
| 4 | Reduced expression of insulin-like growth factor binding protein-3 and its promoter hypermethylation in human hepatocellular carcinoma显示文摘 | Tadashi Hanafusa Yasuhiro Yumoto Kazuhiro Nouso Harushige Nakatsukasa Toru Onishi Tatsuya Fujikawa Mayumi Taniyama Shinichiro Nakamura Masayuki Uemura Yoshitaka Takuma Eiichiro Yumoto Toshihiro Higashi Takao Tsuji | 2002 | Cancer Letters2002，，： | 1 |
| 5 | Differences between Corrosion Products Formed on Copper Exposed in Tokyo in Summer and Winter显示文摘 | MASAMITSU Watanabe YASUHIRO Higashi TOHRU Tanaka | 2003 | Corrosion Science2003，45，： | 1 |
| 6 | Reduced expression of insulin-like growth factor binding protein-3 and its promoter hypermethylation in human hepatocellular carcinoma显示文摘 | Tadashi Hanafusa Yasuhiro Yumoto Kazuhiro Nouso Harushige Nakatsukasa Toru Onishi Tatsuya Fujikawa Mayumi Taniyama Shinichiro Nakamura Masayuki Uemura Yoshitaka Takuma Eiichiro Yumoto Toshihiro Higashi Takao Tsuji | 2002 | Cancer Letters2002，，2： | 1 |
| 7 | Surface Observation and Depth Profiling Analysis Studies of Corrosion Products on Copper Exposed Outdoors显示文摘 | MASAMITSU Watanabe YASUHIRO Higashi TOSHIHIRO Ichino | 2003 | Journal of the Electrochemical Society2003，150，2： | 1 |
| 8 | Value of concomitant endoscopic balloon dilation for intestinal stricture during long‐term infliximab therapy in patients with crohn’s disease显示文摘 | Yoichiro Ono Fumihito Hirai Toshiyuki Matsui Takahiro Beppu Yutaka Yano Noritaka Takatsu Yasuhiro Takaki Takashi Nagahama Takashi Hisabe Kenshi Yao Daijiro Higashi Kitaro Futami | 2012 | Digestive Endoscopy2012，，6： | 1 |
| 9 | Differences between corrosion products formed on copper exposed in Tokyo in summer and winter显示文摘 | WATANABE Masamitsu HIGASHI Yasuhiro Tohru Tanaka | 2003 | Corrosion Science2003，45，： | 1 |
| 10 | Surface observation and depth profiling analysis studies of corrosion products on copper exposed outdoors显示文摘 | WATANABE Masamitsu HIGASHI Yasuhiro ICHINO Toshihiro | 2003 | Journal of the Electrochemical Society2003，150，2： | 1 |
| 11 | Potassium transporter KUP9 participates in K+distribution in roots and leaves under low K+stress显示文摘Potassium(K)is a major essential element in plant cells,and KUP/HAK/KT-type K+transporters participate in the absorption of K+into roots and in the long-distance transport to above-ground parts.In Arabidopsis thaliana,KUP9 is involved in the transport of K+and Cs+in roots.In this study,we investigated KUP9 function in relation to the K+status of the plant.The expression of KUP9 was upregulated in older leaves on K+-depleted medium,compared to the expression of the other 12 KUP genes in the KUP/HAK/KT family in Arabidopsis.When grown on low K+medium,the kup9 mutant had reduced chlorophyll content in seedlings and chlorosis in older rosette leaves.Tissue-specific expression of KUP9 determined by KUP9 promoter:GUS assay depended on the K+status of the plants:In K+sufficient medium,KUP9 was expressed in the leaf blade towards the leaf tip,whereas in K+depleted medium expression was mainly found in the petioles.In accordance with this,K+accumulated in the roots of kup9 plants.The short-term 43K+tracer measurement showed that 43K was transferred at a lower rate in roots and shoots of kup9,compared to the wild type.These data show that KUP9 participates in the distribution of K+in leaves and K+absorption in roots under low K+conditions. | Taro Yamanashi Takeshi Uchiyama Shunya Saito Taiki Higashi Hayato Ikeda Hidetoshi Kikunaga Mutsumi Yamagami Yasuhiro Ishimaru Nobuyuki Uozumi | 2022 | Stress Biology2022，2，1： | 0 |
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