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| 1 | Heterosis and combining ability evaluation for growth traits of blunt snout bream(Megalobrama amblycephala)when crossbreeding three strains显示文摘The blunt snout bream(Megalobrama amblycephala)is a major aquaculture species in the Chinese freshwater polyculture system.In this study,complete diallel crossing of three strains(i.e.,Liangzi[LZ],Poyang[PY]and Yuni[YN])was used to evaluate the combining ability and heterosis effect of intraspecific crossbreeding on the growth performance(i.e.,body weight,total length,body length,body height,and Fulton’s coefficient of condition).The offspring produced from the three strains were reared in a communal pond for 20 months,and nine microsatellites were used to assess their pedigree.Of the749 offspring,708(94.53%)could be assigned directly to a single parental pair.Significant differences were observed in body weight(BW)among the different combinations,indicating that the combining ability of BW from each strain was significantly different.The general combining ability(GCA)of BW from the sire was much higher than that from the dam.The GCA for the dam and sire of the LZ and YN were the largest and the smallest,respectively.The special combining ability of YN$9 PY#was the largest,and YN$9 PY#showed significantly positive heterosis effects on all F1growth traits,except for Fulton’s condition coefficient(K)(P\0.05).In addition,significant positive linear correlations were found between the mean BW of corresponding progeny and the H E of the parental generation(y=600.7x-29.472,r=0.8651,P=0.0026),H O(y=1206.9x-518.14,r=0.7436,P=0.0216),and the polymorphism information content(y=1021.3x-331.31,r=0.8245,P=0.0063)as determined by a correlative test.The findings obtained from the study will be important for future considerations of M.amblycephala stock improvement programs. | Wei Luo Cong Zeng Shaokui Yi Nicholas Robinson Weimin Wang Zexia Gao | 2014 | Chinese Science Bulletin2014，59，9： | 7 |
| 2 | Effects of Silver Nanoparticles on Denitrification and Associated N_(2)O Release in Estuarine and Marine Sediments显示文摘Silver nanoparticles(AgNPs)have been widely used in medicine and consumer products.And it enters the river in dif-ferent ways,then finally converges to the ocean through the estuary.AgNPs polution can affect NO_(2)−and N_(2)O production by denitri-fiers in aquatic system.The effects of AgNPs on denitrification activity,nitrogen transformation and nitrous oxide(N_(2)O)emission were investigated in Dagu River Estuary(DRE)and Jiaozhou Bay(JZB).The results showed that the potential denitrification activity(PDA),NO_(3)−and NO_(2)−reduction rates decreased with an increase of AgNPs concentration in DRE and JZB.However,the N_(2)O ac-cumulation was significantly increased at AgNPs concentrations above 5 mg kg^(−1)in both areas,and the accumulation rate was greater in estuary than in bay(P<0.05).Moreover,the total bacterial count showed a slightly increasing trend with an increase of AgNPs concentration(P>0.05)in DRE and JZB.Importantly,the relative abundance of narG,nirS and nosZ gene in two areas decreased with the increase of AgNPs concentration,and the negative effect of AgNPs varied in order:nosZ>nirS>narG,inferring that the expression of denitrifying related genes could be significantly and differently inhibited by AgNPs addition.Thus,this study demon-strated that the inhibitory effect of AgNPs on different denitrification process,which may lead to the increase of inorganic nitrogen accumulation and N_(2)O realease.This study provides a scientific basis for the further studies of AgNPs on the ecological impact mechanism and environmental effects of offshore sedimentary environment. | SUN Pengfei LI Kuiran YI Shaokui LI Hui CHEN Xi | 2022 | Journal of Ocean University of China2022，21，1： | 0 |
| 3 | Research on Multi-View Image Reconstruction Technology Based on Auto-Encoding Learning显示文摘Traditional three-dimensional(3D)image reconstruction method,which highly dependent on the environment and has poor reconstruction effect,is easy to lead to mismatch and poor real-time performance.The accuracy of feature extraction from multiple images affects the reliability and real-time performance of 3D reconstruction technology.To solve the problem,a multi-view image 3D reconstruction algorithm based on self-encoding convolutional neural network is proposed in this paper.The algorithm first extracts the feature information of multiple two-dimensional(2D)images based on scale and rotation invariance parameters of Scale-invariant feature transform(SIFT)operator.Secondly,self-encoding learning neural network is introduced into the feature refinement process to take full advantage of its feature extraction ability.Then,Fish-Net is used to replace the U-Net structure inside the self-encoding network to improve gradient propagation between U-Net structures,and Generative Adversarial Networks(GAN)loss function is used to replace mean square error(MSE)to better express image features,discarding useless features to obtain effective image features.Finally,an incremental structure from motion(SFM)algorithm is performed to calculate rotation matrix and translation vector of the camera,and the feature points are triangulated to obtain a sparse spatial point cloud,and meshlab software is used to display the results.Simulation experiments show that compared with the traditional method,the image feature extraction method proposed in this paper can significantly improve the rendering effect of 3D point cloud,with an accuracy rate of 92.5%and a reconstruction complete rate of 83.6%. | Tao Zhang Shaokui Gu Jinxing Niu Yi Cao | 2022 | Computers, Materials & Continua2022，，9： | 0 |
| 4 | An Efficient Trap Passivator for Perovskite Solar Cells:Poly(propylene glycol)bis(2‑aminopropyl ether)显示文摘Perovskite solar cells(PSCs)are regarded as promising candidates for future renewable energy production.High-density defects in the perovskite films,however,lead to unsatisfactory device performances.Here,poly(propylene glycol)bis(2-aminopropyl ether)(PEA)additive is utilized to passivate the trap states in perovskite.The PEA molecules chemically interact with lead ions in perovskite,considerably passivate surface and bulk defects,which is in favor of charge transfer and extraction.Furthermore,the PEA additive can efficiently block moisture and oxygen to prolong the device lifetime.As a result,PEA-treated MAPbI3(MA:CH3NH3)solar cells show increased power conversion efficiency(PCE)(from 17.18 to 18.87%)and good longterm stability.When PEA is introduced to(FAPbI3)1-x(MAPbBr3)x(FA:HC(NH2)2)solar cells,the PCE is enhanced from 19.66 to 21.60%.For both perovskites,their severe device hysteresis is efficiently relieved by PEA. | Ningli Chen Xiaohui Yi Jing Zhuang Yuanzhi Wei Yanyan Zhang Fuyi Wang Shaokui Cao Cheng Li Jizheng Wang | 2020 | Nano-Micro Letters2020，12，12： | 0 |
| 5 | Genetic parameter evaluation for growth and cold resistance traits of the giant freshwater prawn Macrobrachium rosenbergii显示文摘The giant freshwater prawn Macrobrachium rosenbergii distributed from tropical to subtropical regions,is a warm-water species,and its survival temperature is 14-35°C,which greatly limits its culture cycle and culture area in China.Therefore,it is urgent to cultivate a new high quality,high yield variety with improved cold-resistance,but the genetic parameters for cold-resistance traits are unknown in M.rosenbergii.In this study,the cold-resistance of adult M.rosenbergii populations was tested using the indoor artificial cooling method.Individuals were selected from 139 families of Shufeng G3 generation and cultured for 200 days.A linear mixed model was constructed by ASReml-R to evaluate the genetic parameters of the cold-resistance trait(cooling degree hours,CDH)and growth traits(body weight,BW,and body length,BL)based on the restricted maximum likelihood(REML)method.The results show that the heritability of CDH was low(0.12±0.05),while the growth traits(BW and BL)had low to moderate heritability,with 0.20±0.06 for BW and 0.06±0.04 for BL.The phenotypic and genetic correlation between BW and BL was significantly positive,but significantly negative phenotypic and genetic correlations were detected between CDH and BW and between CDH and BL.Furthermore,the analysis of the differences between cold-resistance and phenotypic traits showed that the female reproductive status,exoskeleton hardness and claw number of adult prawns had a great influence on the cold-resistance of M.rosenbergii(P<0.05),indicating that adults with claws and hard exoskeletons are preferred as parents in subsequent breeding selection.The present results can be attributed to the selection and breeding of a new cold-resistant variety of M.rosenbergii. | Haihui TU Qianqian XING Zhenxiao ZHONG Qiongying TANG Shaokui YI Zhenglong XIA Miaoying CAI Jingfen LI Quanxin GAO Guoliang YANG | 2024 | Journal of Oceanology and Limnology2024，42，2： | 0 |
| 6 | Isolation,identification,and virulence gene analysis of pathogenic Aeromonas dhakensis in Macrobrachium rosenbergii and histopathological observation显示文摘To identify the cause of mass mortality of adult Macrobrachium rosenbergii in a farm in Gaoyou City,Jiangsu Province,China,a dominant strain named DKQ-1 was isolated from the hepatopancreas of dying M.rosenbergii and identified as Aeromonas dhakensis by purification culture,biochemical characterization,and 16S rRNA and gyrB gene sequence analysis.The results of the challenge test revealed that the strain was highly pathogenic and the 50%lethal dose(LD_(50))in 72 h to M.rosenbergii was 1.54×10^(5)CFU/mL.The amplification results of virulence genes show that strain DKQ-1 carried 9 virulence genes,including ascV,aexT,aer,act,lip,ompAI,gcaT,acg,and exu,supporting the strong virulence of strain DKQ-1 to M.rosenbergii.Histopathological observation of the hepatopancreas,gills,and intestines indicated that DKQ-1 injection into M.rosenbergii could cause serious tissue damage,which further supported the strong virulence of this strain.In addition,a drug susceptibility test revealed that strain DKQ-1 was sensitive to 16 kinds of antibiotics,resistant to 9 kinds of antibiotics,and had intermediate resistance to spectinomycin and kanamycin.This study is the first report of A.dhakensis isolated from M.rosenbergii and provided a reference for the pathogen identification of bacterial diseases in M.rosenbergii,and for the prevention and treatment caused by A.dhakensis. | Xin PENG Haihui TU Xinyi YAO Xuan LAN Zhenxiao ZHONG Jinping LUO Qiongying TANG Shaokui YI Zhenglong XIA Guoliang YANG | 2024 | Journal of Oceanology and Limnology2024，42，2： | 0 |
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