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| 1 | Onco-miR-24 regulates cell growth and apoptosis by targeting BCL2L11 in gastric cancer显示文摘胃的癌症是世界范围的最普通的恶意之一;然而,在 tumorigenesis 的分子的机制仍然需要探索。BCL2L11 属于 BCL-2 家庭,并且充当内在的 apoptotic 串联的一个中央管理者并且调停房间 apoptosis。尽管 miRNAs 被报导了涉及癌症开发的每个阶段,在 GC 的 miR-24 的角色还没被报导。在现在的学习,当 BCL2L11 的表示在 GC 的肿瘤纸巾被禁止时, miR-24 被发现起来调整。研究从在 vitro 并且在显示出的 vivo,那 miR-24 调整 BCL2L11 表示由与 mRNA 的 3UTR 直接有约束力,因此支持房间生长,移植当禁止房间 apoptosis 时。因此, miR-24 是能是为未来的潜在的药目标的新奇 onco-miRNA 临床的使用。 | Haiyang Zhang Jingjing Duan Yanjun Qu Ting Deng Rui Liu Le Zhang Ming Bai Jialu Li Tao Ning Shaohua Ge Xia Wang ZhenzhenWang~ Qian Fan Hongli Li Guoguang Ying Dingzhi Huang Yi Ba | 2016 | Protein & Cell2016，7，2： | 11 |
| 2 | PKM2 promotes reductive glutamine metabolism显示文摘Objective:Pyruvate kinases M(PKM),including the PKM1 and PKM2 isoforms,are critical factors in glucose metabolism.PKM2promotes aerobic glycolysis,a phenomenon known as'the Warburg effect'.The purpose of this study was to identify the roles of PKM2 in regulating cellular metabolism.Methods:The CRISPR/Cas9 system was used to generate the PKM-knockout cell model to evaluate the role of PKM in cellular metabolism.Lactate levels were measured by the Vitros LAC slide method on an autoanalyzer and glucose levels were measured by the autoanalyzer AU5800.The metabolism of ^(13)C_6-glucose or ^(13)C_5-glutamine was evaluated by liquid chromatography/mass spectrometry analyses.The effects of PKM on tumor growth were detected in vivo in a tumor-bearing mouse model.Results:We found that both PKM1 and PKM2 enabled aerobic glycolysis,but PKM2 converted glucose to lactate much more efficiently than PKM1.As a result,PKM2 reduced glucose levels reserved for intracellular utilization,particularly for the production of citrate,and thus increased theα-ketoglutarate/citrate ratio to promote the generation of glutamine-derived acetylcoenzyme A through the reductive pathway.Furthermore,reductive glutamine metabolism facilitated cell proliferation under hypoxia conditions,which supports in vivo tumor growth.In addition,PKM-deletion induced a reverse Warburg effect in tumorassociated stromal cells.Conclusions:PKM2 plays a critical role in promoting reductive glutamine metabolism and maintaining proton homeostasis.This study is helpful to increase the understanding of the physiological role of PKM2 in cancer cells. | Miao Liu Yuanyuan Wang Yuxia Ruan Changsen Bai Li Qiu Yanfen Cui Guoguang Ying Binghui Li | 2018 | Cancer Biology & Medicine2018，15，4： | 6 |
| 3 | Determination of glucose deficiency-induced cell death by mitochondrial ATP generation-driven proton homeostasis显示文摘 | Yanfen Cui Yuanyuan Wang Miao Liu Li Qiu Pan Xing Xin Wang Guoguang Ying Binghui Li | 2017 | Journal of Molecular Cell Biology2017，9，5： | 3 |
| 4 | Exosomal miR-155 from gastric cancer induces cancerassociated cachexia by suppressing adipogenesis and promoting brown adipose differentiation via C/EPBβ显示文摘Objective:The aim of this research was to identify whether exosomes were involved in impairing adipogenesis in cancer-associated cachexia(CAC)by detecting the adipodifferentiation capacity and the expressions of adipogenic proteins in gastric cancer(GC)-associated adipocytes.Methods:Western blotting and RT-PCR were used to investigate the expressions of C/EPBβ,C/EPBα,PPARγ,and UCP1 in adipose mesenchymal stem cells(A-MSCs)to evaluate the function of exosomal miR-155.BALB/c nude mice were intravenously injected in vivo with GC exosomes with different levels of miR-155 to determine changes in adipodifferentiation of A-MSCs.Results:Exosomes derived from GC cells suppressed adipogenesis in A-MSCs as characterized by decreased lipid droplets.Similarly,A-MSCs co-cultured with GC exosomes exhibited increased ATP production through brown adipose differentiation characterized by highly dense mitochondria and enhanced UCP1 expression(P<0.05).Mechanistically,exosomal miR-155 secreted from GC cells suppressed adipogenesis and promoted brown adipose differentiation by targeting C/EPBβ,accompanied by downregulated C/EPBαand PPARγand upregulated UCP1(P<0.05).Moreover,overexpression of miR-155 in GC exosomes improved CAC in vivo,which was characterized by fat loss,suppressed expressions of C/EPBβ,C/EPBα,and PPARγin A-MSCs,and high expression of UCP1(P<0.05).Decreasing the level of miR-155 in injected GC exosomes abrogated the improved CAC effects.Conclusions:GC exosomal miR-155 suppressed adipogenesis and enhanced brown adipose differentiation in A-MSCs by targeting C/EPBβof A-MSCs,which played a crucial role in CAC. | Ying Liu Meng Wang Ting Deng Rui Liu Tao Ning Ming Bai Guoguang Ying Haiyang Zhang Yi Ba | 2022 | Cancer Biology & Medicine2022，19，9： | 3 |
| 5 | Role of aquaporin-4 in the regulation of migration and invasion of humanglioma cells显示文摘 | Ting Ding Yongjie Ma Wenliang Li Xiaoli Liu Guoguang Ying Li Fu Feng Gu | 2011 | International Journal of Oncology2011，，6： | 1 |
| 6 | Sorption and de- sorption behaviors of diuron in soils amended with charcoal 显示文摘 | YU Xiangyang YING Guoguang KOOKANA R S | 2006 | Journal of Agricultural and Food Chemistry2006，，54： | 1 |
| 7 | Potential of electron transfer and its application in dictating routes of biochemical processes associated with metabolic reprogramming显示文摘Metabolic reprogramming,such as abnormal utilization of glucose,addiction to glutamine,and increased de-novo lipid synthesis,extensively occurs in proliferating cancer cells,but the underneath rationale has remained to be elucidated.Based on the concept of the degree of reduction of a compound,we have recently proposed a calculation termed as potential of electron transfer(PET),which is used to characterize the degree of electron redistribution coupled with metabolic transformations.When this calculation is combined with the assumed model of electron balance in a cellular context,the enforced selective reprogramming could be predicted by examining the net changes of the PET values associated with the biochemical pathways in anaerobic metabolism.Some interesting properties of PET in cancer cells were also discussed,and the model was extended to uncover the chemical nature underlying aerobic glycolysis that essentially results from energy requirement and electron balance.Enabling electron transfer could drive metabolic reprogramming in cancer metabolism.Therefore,the concept and model established on electron transfer could guide the treatment strategies of tumors and future studies on cellular metabolism. | Ronghui Yang Guoguang Ying Binghui Li | 2021 | Frontiers of Medicine2021，15，5： | 0 |
| 8 | Cerebrospinal fluid diagnostic markers for two-dimensional electrophoresis-mass spectrometry in Parkinson’s disease patients显示文摘BACKGROUND: Previous studies have confirmed the existence of specific proteins in body fluid of Parkinson’s disease (PD) patients. However, the existing research has contained several interference factors with poor reproducibility and has not focused on patients grouped according to disease duration. OBJECTIVE: To verify differential expression of proteins in cerebrospinal fluid of PD patients grouped in order of disease severity through the use of two-dimensional electrophoresis-mass spectrometry methods. DESIGN, TIME AND SETTING: The proteomic-based, case-control study was performed between September 2008 and June 2009 at the Key Laboratory of Neurology in the First Affiliated Hospital of Chongqing Medical University. PARTICIPANTS: A total of 52 outpatients and/or inpatients, who were admitted to the Department of Neurology in the First Affiliated Hospital of Chongqing Medical University between 2008 and 2009, were randomized into the present study. Among them, 27 PD patients served as the PD group and were assigned to three subgroups according to modified Webster, Hoehn, and Yahr rating scales: 14 = mild, 8 = moderate, and 5 = severe; non-PD group of 16 patients included 5 cases of viral meningitis, 3 cases of acute myelitis, 1 case of Guillain-Barre syndrome, 2 cases of tuberculous meningitis, 2 cases of restless legs syndrome, and 3 cases of essential tremor; control group (n = 9) consisted of muscular tension headache in 6 cases, as well as syncope, trigeminal neuralgia, idiopathic orthostatic hypotension in 1 case. METHODS: Cerebrospinal fluid was collected from the involved patients using the lumbar puncture method. Proteins in the cerebrospinal fluid were separated by two-dimensional electrophoresis. MAIN OUTCOME MEASURES: Characteristics of protein electrophoresis patterns were analyzed, differentially expressed proteins were detected using matrix-assisted laser desorption ionization time of flight mass spectrometry, and protein data were analyzed in the Mascot database. RESULTS: Five protein electropherograms were analyzed by PDQuest 8.0, and (789 ± 32) protein spots were observed. There were significant differences in four protein spots in each of the PD sub-groups compared with the non-disease and control groups. Expression was down-regulated in three protein spots and up-regulated in one protein spot; 100% repetition rate was observed in four protein spots. According to the Mascot database, protein spots with down-regulated expression were as follows: DNA-guided RNA polymerase III subunit RPC5 (score: 50 points); double serine, threonine, and tyrosine protein kinase (score: 64 points, P < 0.05); activity-regulated cytoskeleton-associated protein (score: 58 points, P < 0.05). However, G2 mitotic-specific cyclin was up-regulated (score: 84 points, P < 0.05). CONCLUSION: Differential protein expression in the cerebrospinal fluid of PD patients was detected by two-dimensional electrophoresis-mass spectrometry, revealing changes in DNA-guided RNA polymerase III subunit RPC5, double serine, threonine, and tyrosine protein kinase, activity-regulated cytoskeleton-associated protein, and G2 mitotic cell cyclin, with good reproducibility. | Ying Chen Gang Yu Wenbin Tu Hanchun Long Side Jiang Jincheng Wan Guoguang Peng | 2010 | Neural Regeneration Research2010，5，12： | 0 |
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