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| 1 | AURKB and MAPK involvement in the regulation of the early stages of mouse zygote development显示文摘Aurora kinases have become a hot topic for research as they have been found to play an important role in various stages of mitotic cell division and to participate in malignant conversions of tumors. The participation of Aurora kinases in the regulation of oocyte meiosis has been recently reported, but their participation in mammalian early embryonic development remained unclear. The object of our study was to establish the spatio-temporal expression pattern of Aurora kinase B (AURKB) in mouse zygotes during the first cleavage, to reveal its functions in the early development of mouse zygotes, and to define the involvement of AURKB in mitogen-activated protein kinase (MAPK) signaling. Our results showed that in mouse zygotes AURKB expression increased in G1 phase and peaked in M phase. AURKB protein distribution was found to be in association with nuclei and distributed throughout the cytoplasm in a cell cycle-dependent manner. Functional disruption of AURKB resulted in abnormal division phenotypes or mitotic impairments. U0126, a specific mitogen-activated protein kinase kinase (MEK) inhibitor, caused significantly altered morphologies of early embryos together with a decrease in protein expression and kinase activity of AURKB. Our results indicated that the activity of AURKB was required for regulating multiple stages of mitotic progression in the early development of mouse zygotes and was correlated with the activation of the MAPK pathway. | XU Lin LIU Tong HAN Feng ZONG ZhiHong WANG GuoLi YU BingZhi ZHANG Jie | 2012 | Science China(Life Sciences)2012，55，1： | 2 |
| 2 | Novel protein kinase C inhibitor in bovine spermatozoa and its effects on the development of fertilized ova显示文摘 | Bingzhi Yu Haihui Xue | 1995 | Biomedical Research1995，16，3： | 1 |
| 3 | The inhibitory effect of BSP-A1/-A2 on protein kinase C and tyrosine protein kinase 显示文摘 | Bingzhi Yu Yongmei Zhao | 2003 | Cell Biochem Funct2003，21，2： | 1 |
| 4 | Distinct signaling events downstream of mTOR cooperate to mediate the effects on oral squamous cell carcinoma and Hela cells显示文摘 | Liu Yi Yu Bingzhi | 2006 | Cell Biochemistry and function2006，24，： | 1 |
| 5 | High expression of ncRAN, a novel non-coding RNA mapped to chromosome17q25.1, is associated with poor prognosis in neuroblastoma显示文摘 | Meng Yu Miki Ohira Yuanyuan Li Hidetaka Niizuma Myat Oo Yuyan Zhu Toshinori Ozaki Eriko Isogai Yohko Nakamura Tadayuki Koda Shigeyuki Oba Bingzhi Yu Akira Nakagawara | 2009 | International Journal of Oncology2009，，4： | 1 |
| 6 | Adsorption of bisphenol A by polysulphone membrane显示文摘 | Wu Suhua Dong Bingzhi Huang Yu | | 0，，： | 1 |
| 7 | Involvement of protein kinase B/AKT in early development of mouse fertilized eggs显示文摘 | Chen Feng Aiming Yu Bingzhi Yu | 2007 | Biolreprod2007，77，3： | 1 |
| 8 | Corneal thickness and elevation measurements using swept‐source optical coherence tomography and slit scanning topography in normal and keratoconic eyes显示文摘 | Vishal Jhanji Bingzhi Yang Marco Yu Cong Ye Christopher KS Leung | 2013 | Clin Experiment Ophthalmol2013，，8： | 1 |
| 9 | Corneal thickness and elevation measurements using swept‐source optical coherence tomography and slit scanning topography in normal and keratoconic eyes显示文摘 | Vishal Jhanji Bingzhi Yang Marco Yu Cong Ye Christopher KS Leung | 2013 | Clin Experiment Ophthalmol2013，，8： | 1 |
| 10 | Involvement of insulin in early development of mouse one-cell stage embryos显示文摘Recent studies have suggested that growth factors and hormones play important roles in cell prolif-eration and differentiation during early embryonic development. In the present study, we examined the expression and localization of insulin in the mouse oocytes and one-cell stage embryos by quantitative ELISA, RT-PCR, Western blot and immunofluorescence. In the mouse oocytes and one-cell stage em-bryos, expression of insulin was uniformly distributed in the cytoplasm. We also examined the expres-sion, activity and localization of mTOR (mammalian target of rapamycin) and p70S6K. The expression of mTOR and p70S6K was not significantly different at the cell cycle of mouse one-cell stage embryos. mTOR and S6K were distributed evenly in the cytoplasm at G1, G2 and M phase phase, but at S phase, the distribution of mTOR and S6K was around the pronucleus. At different phases, the activity of mTOR fluctuated. We also used the PI3K specific inhibitor-Wortmannin to investigate the cleavage rate of eggs. The result showed that the rate obviously decreased. When the mTOR specific inhibitor Rapa-mycin was used, the first mitotic division of the mouse one-cell stage embryo was delayed. These re-sults suggested that insulin was expressed both in mouse oocytes and one-cell stage embryos, and may play functional roles in regulation of mouse early embryogenesis by activating the signal pathway of PI3K/PKB/mTOR/S6K. | YU BingZhi, ZHANG Zhe, DENG Xin, XU XiaoYan, FENG Chen, LI YanXiao, CUI Cheng, SU WenHui, ZHAO HongMei & YU DaHai Department of Biochemical and Molecular Biology, China Medical University, Shenyang 110001, China | 2008 | Science China(Life Sciences)2008，51，9： | 1 |
| 11 | Optic Disc Imaging with Spectral-Domain Optical Coherence Tomography显示文摘 | Bingzhi Yang Cong Ye Marco Yu Shu Liu Dennis Shun Chiu Lam Christopher Kai-shun Leung | 2012 | Ophthalmology2012，，9： | 1 |
| 12 | Binary trinuclear metal-oxo sub-nanomaterials for photocatalytic hydrogen and chlorine production from seawater显示文摘Owing to the need for regenerant and self-reduction problem,the hydrogen performance of sub-nano-sized trinuclear iron-oxo complexes is still far from satisfied with affordability and practicality.Herein,two binary photocatalytic systems based on trinuclear metal-oxo complexes have been first constructed and experimentally confirmed to be competent for seawater hydrogen evolution(715.4and271.9μmol of hydrogen can be found,respectively,after 48h).Notably,chloride ions act as the hole catcher and move into the gas phase in the stable form of chlorine.Similar to heterogeneous structures,homogeneous systemsnot only enhance the hydrogen performance while ensuring the stability of metal-oxo complexes,but also shorten the consumption of photogenerated carriers by dissolved impurities in the seawater.This new attempt of building pluralistic sub-nanometric systems may offer novel design strategies with noble-metal-free catalysts and low-cost candidates for traditional semiconductor materials in enhancing photocatalytic efficiency and performing chlorine evolution from seawater splitting. | Yang Wang Litong Shi Haijun Hu Bingzhi Qian Wei Hou Hui Li Xue Liu Daliang Liu Shuyao Wu Hongwei Huang Xi-Ming Song Yu Zhang Tianyi Ma | 2022 | SusMat2022，2，6： | 0 |
| 13 | Antifungal effects of BiOBr nanosheets carrying surfactant cetyltrimethylammonium bromide显示文摘BiOBr nanosheets are important photocatalytic nanomaterials. However, their biological effects remain to be explored. In this study, we investigated the antifungal effect of BiOBr nanosheets on Candida albicans. Strikingly,the nanosheets strongly inhibited the growth of C. albicans [IC_(50)=(96±4.7) mg/L],hyphal development and biofilm formation. Compareed to the antifungal effect of the cationic surfactant cetyltrimethylammonium bromide, the inhibitory effect of the nanosheets on fungal pathogen was attributed to cetyltrimethylammonium bromide adsorbed by the nanosheets. Thermal gravity analysis and cetyltrimethylammonium bromide release experiment indicated that only 0.42% cetyltrimethylammonium bromide on BiOBr nanosheets was released. Taken together, this study uncovers the contribution of surfactant released from the nanosheets to their antifungal activity. | Meiqing Sun Zhanlin Ding Hong Wang Guangping Yu Bingzhi Li Mingchun Li Mengmeng Zhen | 2018 | The Journal of Biomedical Research2018，32，5： | 0 |
| 14 | Gelatin filter capture-based high-throughput sequencing analysis of microbial diversity in haze particulate matter显示文摘Airborne particulate matter(PM),especially PM2.5,can be easily adsorbed by human respiratory system.Their roles in carrying pathogens for spreading epidemic diseases has attracted great concern.Herein,we developed a novel gelatin filter-based and culture-independent method for investigation of the microbial diversity in PM samples during a haze episode in Tianjin,China.This method involves particle capture by gelatin filters,filter dissolution for DNA extraction,and high-throughput sequencing for analysis of the microbial diversity.A total of 584 operational taxonomic units(OTUs)of bacteria and 370 OTUs of fungi at the genus level were identified during hazy days.The results showed that both bacterial and fungal diversities could be evaluated by this method.This study provides a convenient strategy for investigation of microbial biodiversity in haze,facilitating accurate evaluation of airborne epidemic diseases. | Meiqing Sun Zhanlin Ding Hong Wang Guangping Yu Zhe Feng Bingzhi Li Penghui Li | 2019 | The Journal of Biomedical Research2019，33，6： | 0 |
| 15 | Batch synthesis of transfer-free graphene with wafer-scale uniformity显示文摘Scalable synthesis of transfer-free graphene over insulators offers exciting opportunity for next-generation electronics and optoelectronics.However,rational design of synthetic protocols to harvest wafer-scale production of directly grown graphene still remains a daunting challenge.Herein we explore a batch synthesis of large-area graphene with wafer-scale uniformity by virtue of direct chemical vapor deposition(CVD)on quartz.Such a controllable CVD approach allows to synthesize 30 pieces of 4-inch graphene wafers in one batch,affording a low fluctuation of optical and electrical properties.Computational fluid dynamics simulations reveal the mechanism of uniform growth,indicating thermal field and confined flow field play leading roles in attaining the batch uniformity.The resulting wafer-scale graphene enables the direct utilization as key components in optical elements.Our method is applicable to other types of insulating substrates(e.g.,sapphire,SiO2/Si,Si3N4),which may open a new avenue for direct manufacture of graphene wafers in an economic fashion. | Bei Jiang Qiyue Zhao Zhepeng Zhang Bingzhi Liu Jingyuan Shan Liang Zhao Mark H.Rümmeli Xuan Gao Yanfeng Zhang Tongjun Yu Jingyu Sun Zhongfan Liu | 2020 | Nano Research2020，13，6： | 0 |
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