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| 1 | The cloning and preliminarily functional analysis of the human neurotrimin gene显示文摘Proteins of the immunoglobulin superfamily (IgSF) are involved in a variety of spe- cific cell-cell interactions in the developing nervous system. To identify and characterize new members of this protein family in human nervous system, we screen the human fetal brain cDNA library and isolate a full-length cDNA clone which contains a 1032 bp open reading frame en- coding a protein of 344 amino acids. Sequence analysis reveals that it is a glycoprotein com- prised of three C2-1ike immunoglobulin domains and is anchored to the plasma membrane via a post-translationally attached glycosyl-phosphatidylinositol (GPI) moiety. The protein shows high sequence similarity to the rat Ntm (97%), so we term it human neurotrimin (NTM). Northern blot analysis reveals that (HUMAN)NTM has three different transcripts with the length of 3.2 kb, 4.0 kb and 9.0 kb respectively. It has a wider expression pattern than that of (RAT) Ntm. Notably, the expression of NTM in fetal brain is higher than that in mature brain and is stronger in nervous tumors than that in normal brain tissues. We insert an HA epitope tag between the third Ig-like domain of NTM and the site of GPI attachment, then construct it into the eukaryotic expression vector pcDNA3.1+/Zeocin. The pcDNA3.1-HA-NTM is transfected into the Chinese Hamster Ovary (CHO) cells. The results demonstrate that HA-NTM is expressed on the surface of CHO cells and could strengthen the aggregation of CHO-NTM cells. | LIU Jin, LI Guangtao, PENG Xiaozhong, LIU Bingyan, YIN Bin, TAN Xinyu, FAN Ming, FAN Wenhong, QIANG Boqin & YUAN Jiangang National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine, Peking Union Medical College, National Human Genome North Center, Beijing 100005, China Department of Neuro- biology, Institute of Basic Medical Science, 27 Taiping Road, Beijing 100850, China | 2004 | Science China(Life Sciences)2004，47，2： | 1 |
| 2 | Application of an atmospheric pressure plasma jet in a rat model of ischaemic stroke:Design,optimisation,and characteristics显示文摘Recent studies have shown the in vitro neuro-protective functions of atmospheric pressure plasma(APP)against multiple pathological injuries during ischaemic stroke(IS).However,whether APP treatment exerts a therapeutic effect on a rat IS model remains unclear.Here,on the basis of needle-to-ring dielectric barrier discharge,an atmospheric pressure plasma jet(APPJ)was designed,with the Helium as the working gas which was driven by a sinusoidal voltage.Then,the treatment conditions were optimised for IS rat model treatment and the characteristics of this APPJ were further diagnosed.Subsequently,the rat IS model was established through 90 min middle cerebral artery occlusion(MCAO),and plasma was intermittently inhaled by rats via the nasal cavity for a 2 min period at 60 min of MCAO process.The therapeutic effects of this plasma jet device were then evaluated using biomedical analyses.According to our results,intermittent APP inhalation in the MCAO rats increased the serum NO content,improved the neurological function,enhanced regional cerebral blood flow,lowered brain infarction,and reduced the cell apoptosis in brain tissues of MCAO rats.Collectively,our data provides a novel potential strategy for IS treatment by using atmospheric-pressure plasma inhalation. | Ye Chen Bingyan Yang Yuqing Liu Lixin Xu Zhongfang Shi Yixiao Liu Ruoyu Han Fang Yuan Jiting Ouyang Xu Yan Kostya(Ken)Ostrikov | 2023 | High Voltage2023，8，2： | 0 |
| 3 | Improvement of interleaving Aramid pulp microfibers on compressive strengths of carbon fiber reinforced polymers with and without impact显示文摘Compressive strengths and elastic moduli of Carbon Fiber Reinforced Polymer(CFRP)composites can be noticeably improved by multiple ultra-thin interlays with non-woven Aramid Pulp(AP)micro/nano-fibers.10-ply CFRP specimens with 0,2,4,6,8 g/m^(2)AP were tested under uniaxial compression.Those flexible AP fibers,filling the resin-rich regions and further constructing the fiber bridging at the ply interfaces,can effectively suppress delamination growth and lead to very good improvements both in the compressive strength and the elastic modulus.The CFRP specimen with an optimum interlay thickness has a distinct shear failure mode instead of the typical delamination cracking along the direction of continuous carbon fibers.Compressive Strengths After Impacts(CAI)of 12.35 J were also measured,up to 90%improvement in CAI has been observed.It is concluded those ultra-thin interlays of non-woven AP micro/nano-fibers are beneficial to design and manufacture“high strength”CFRP composites. | Fei CHENG Guangming YANG Yunsen HU Bingyan YUAN Xiaozhi HU | 2023 | Chinese Journal of Aeronautics2023，36，10： | 0 |
| 4 | The multi-scale flow behaviors of sisal fiber reinforced composites during resin transfer molding process显示文摘The flow behaviors of the resin during the resin transfer molding(RTM) process of sisal fiber reinforced composites was studied at different scales with the consideration of the unique hierarchical and lumen structures of sisal fibers compared to those of manmade fibers. The work mainly focused on the development of the multi-scale flow models which include the resin flow inside lumens, intra-bundles and inter-bundles. The models not only quantified the lumen flow based on the Hagen-Poiseuille equation,but also ensured the continuity of the velocity and stress on the boundaries between intra-bundle and inter-bundle regions by applying Brinkman equation. Three dedicated experiments were designed and implemented to validate the effectiveness of the proposed models. The absorbed resin mass over the infiltration time obtained from the single sisal fiber and sisal fiber bundle infiltration experiments showed good agreement with the calculated curves. In terms of the RTM process, the dynamic flow front of the resin was perfectly predicted by the proposed model at macro-scale. | YIN Tao LI Yan YUAN BingYan | 2018 | Science China(Technological Sciences)2018，61，12： | 0 |
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