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| 1 | Highly efficient differentiation of human ES cells and iPS cells into mature pancreatic insulin-producing cells显示文摘人的 pluripotent 干细胞代表功能的胰腺的内分泌的系房间的潜在地无限的来源。这里,我们报导一条高度有效的途径导致人的胚胎的茎(ES ) 细胞和导致的 pluripotent 茎(iPS ) 在一个定义化学药品的文化系统区分进成熟生产胰岛素的细胞的细胞。这条途径获得的区分的人的 ES 房间由流动 cytometry 分析作为 assayed 包括了将近 25% 胰岛素积极的房间,它以比得上成年人的小岛的一种方式响应葡萄糖刺激释放了 insulin/C-peptide。大多数这些生产胰岛素的房间共同表示成熟尾房间特定的标记象 NKX6-1 和 PDX1 那样,在 vivo 显示一个类似的基因表示模式到成年小岛尾房间。在这研究,我们也证明 EGF 便于 PDX1 积极的胰腺的祖先的扩大。而且,我们的协议也成功了高效地导致人的 iPS 房间区分进生产胰岛素的房间。因此,这个工作不仅提供一个新模型在 vitro 学习人的胰腺的专门化和成熟的机制,而且提高为糖尿病的处理利用病人特定的 iPS 房间的可能性。 | Donghui Zhang Wei Jiang Meng Liu Xin Sui Xiaolei Yin Song Chen Yan Shi Hongkui Deng | 2009 | Cell Research2009，19，4： | 90 |
| 2 | Deformation analysis of the flexspline of harmonic drive gears considering the driving speed effect using laser sensors显示文摘Accurate description of the elastic deformation of the flexsphne is the foundation for optimization design of the structure and conjugate profiles of the harmonic drive gear.This paper proposed an experimental method to investigate the effect of the driving speed on the deformation characteristics of the flexspline.First,an experimental apparatus that integrates a special-fabricated micro-displacement platform and a pair of laser displacement sensors is developed,and the radial displacement of the flexsphne is measured in vertical and horizontal directions.Next,the deformation analyses of the flexsphne at different driving speeds are performed with our method and the conventional method,and the comparison results reveal that the radial displacement of the flexsphne is actually composed of both harmonic and random components,and the amplitude decreases and tends to zero with the increase of the driving speed,especially near the closed end of the flexsphne.Last,the mechanisms of the inherent multi-frequency and amplitude attenuation characteristics of the radial displacement of the flexsphne are discussed.It is indicated that the impact and friction existing in the flexible bearing of the wave generator is likely responsible for the existence of the random component,and the assumption of linear distribution of the flexsphne deformation along the rotating axis is invalid under high speed condition.Our research promotes the further study on the contact-impact problem of the flexible bearing of the wave generator and the transfer characteristic of the elastic deformation of the flexsphne. | MA DongHui WU JiaNing LIU Tao YAN ShaoZe | 2017 | Science China(Technological Sciences)2017，60，8： | 8 |
| 3 | A method for detection and quantification of meshing characteristics of harmonic drive gears using computer vision显示文摘A lack of accurate description of the meshing characteristics and the corresponding frictional mechanism of the harmonic drive gear has limited progress toward modeling the hysteresis stiffness. This paper presents a method for detection and quantification of the meshing characteristics of the harmonic drive gear based on computer vision. First, an experimental set-up that integrates a high speed camera system with a lighting system is developed, and the image processing is adopted to extract and polish the tooth profiles of the meshed teeth pairs in each acquired video sequence. Next, a physical-mathematical model is established to determine the relative positions of the selected tooth pair in the process of the gear engagement, and the combined standard uncertainty is utilized to evaluate the accuracy of the calculated kinematics parameters. Last, the kinematics analysis of the gear engagement under the ultra-low speed condition is performed with our method and previous method, and the influence of the input rotational speed on the results is examined. The results validate the effectiveness of our method, and indicate that the conventional method is not available in the future friction analysis. It is also shown that the engaging-in phase is approximately a uniform motion process, the engaging-out phase is a variable motion process, and these characteristics remain unchanged with the variation of the input rotational speed. Our method affords the ability to understand the frictional mechanism on the meshed contact surfaces of the harmonic drive gear, and also allows for the dynamic monitoring of the meshing properties. | MA DongHui WU JiaNing YAN ShaoZe | 2016 | Science China(Technological Sciences)2016，59，9： | 7 |
| 4 | Management Strategies and Research Orientations of Forest Diseases and Pests in China显示文摘Forest diseases and pests are perceived as a growing hazard to China economy. It is a common conclusion that the actualities of forest pests in china are no effective measures to the old important pests, some secondary pests are ascending to chief pests, increasing devastation from exotic pests, frequent ecological pest eruption induced by environmental detriment and host-leading diseases to threaten the 'Western Development Project 'in China, which is the most important economical strategy to China; th... | ZHANG Xingyao LIANG Jun LU Quan YAN Donghui LUO Youqing XU Zhichun WEN Junbao1. Research Institute of Forest Ecology. Environment and Protection. Chinese Academy of Forestry. Beijing, 100091. P R China:2. College of Biological Sciences and Technology. Beiiine Forestry University. Beiiine. 100083. P R China | 2002 | Chinese Forestry Science and Technology2002，1，2： | 4 |
| 5 | Parametrization of woods-saxon potential for heavy-ion systems显示文摘Several elastic scattering angular distributions of ^(12)C from target nuclei of A ≥39 are analyzed to extract the Woods-Saxon potential parameters with the fixed imaginary potential and Coulomb radius parameters.Using the best fitted diffuseness parameters,the correlations of the real part parameters with A_1^(1/3)+ A_2^(1/3) and incident energy are revealed, and the systematic Woods-Saxon potential parameters are presented for nucleus-nucleus interaction.The proposed potential parameters can reproduce not only the elastic scattering angular distributions induced by ^(12)C,but also many elastic scattering angular distributions induced by the projectiles other than ^(12)C,thus providing important inputs for the study of nuclear reactions of heavy-ion systems. | Lin Gan ZhiHong Li HuiBin Sun DanYang Pang Bing Guo YunJu Li Jun Su ShengQuan Yan ErTao Li YouBao Wang Gang Lian ZhiYu Han XinYue Li DongHui Li TianLi Ma ChangJin Pei YangPing Shen Yi Su Sheng Zeng Yong Zhou WeiPing Liu | 2017 | Science China(Physics,Mechanics & Astronomy)2017，60，8： | 2 |
| 6 | Temporal and spatial changes of microbial community in an industrial effluent receiving area in Hangzhou Bay显示文摘Anthropogenic activities usually contaminate water environments, and have led to the eutrophication of many estuaries and shifts in microbial communities. In this study, the temporal and spatial changes of the microbial community in an industrial effluent receiving area in Hangzhou Bay were investigated by 454 pyrosequencing. The bacterial community showed higher richness and biodiversity than the archaeal community in all sediments. Proteobacteria dominated in the bacterial communities of all the samples; Marine_Group_Ⅰ and Methanomicrobia were the two dominant archaeal classes in the effluent receiving area. PCoA and AMOVA revealed strong seasonal but minor spatial changes in both bacterial and archaeal communities in the sediments. The seasonal changes of the bacterial community were less significant than those of the archaeal community, which mainly consisted of fluctuations in abundance of a large proportion of longstanding species rather than the appearance and disappearance of major archaeal species. Temperature was found to positively correlate with the dominant bacteria, Betaproteobacteria, and negatively correlate with the dominant archaea,Marine_Group_Ⅰ; and might be the primary driving force for the seasonal variation of the microbial community. | Yan Zhang Lujun Chen Renhua Sun Tianjiao Dai Jinping Tian Wei Zheng Donghui Wen | 2016 | Journal of Environmental Sciences2016，28，6： | 2 |
| 7 | Advances in Researches on Chemical Composition and Functions of Hemerocallis Plants显示文摘The genus Hemerocallis is a kind of perennial herbaceous plants of Liliaceae and consists of about 15 species in the world,11 of which can be found in China. Some species of Hemerocallis are widely cultivated for medicinal,edible and ornamental application. Considerable progress had been made in researches on chemical composition and functions of Hemerocallis plants. Previous phytochemical studies on Hemerocallis plants have demonstrated the presence of flavonoids,anthraquinones,alkaloids,terpenoids,triterpenes and triterpenoid saponins,caffeoylquinic acid derivatives,naphthalene glycosides,steroid and steroidal saponins,phenylethanoid glycosides,and lignans. Functional activities of Hemerocallis plants are mainly manifested in sedative hypnotic,antidepressant,antioxidant,anti-tumor,hepatoprotective,antibacterial and insecticidal activities. Chemical composition of Hemerocallis plants is various,and structural type is rich,and has a variety of significant functional activities,so it is worth further study. | Yan WANG Teng XU Bei FAN Lijing ZHANG Cong LU Donghui WANG Xinmin LIU Fengzhong WANG | 2018 | Medicinal Plant2018，9，2： | 2 |
| 8 | Surface alloyed Ti–Zr layer constructed on titanium by Zr ion implantation for improving physicochemical and osteogenic properties显示文摘Titanium has been widely used as dental and orthopedic implant, while its corrosion resistance and osteogenic properties need to be further improved. Ti–Zr alloy is considered as one of promising titanium alloy with good corrosion resistance and biocompatibility. In this work, zirconium ions are implanted into the titanium surface by plasma immersion ion implantation(PIII) technology to construct Ti–Zr alloy layer on titanium surface with different proportions of Ti/Zr. The mechanical property, corrosion resistance and cytocompatibility of modified titanium were studied. Results indicated that Ti–Zr alloy layer on titanium exhibited good mechanical property and corrosion resistance. It could promote the cell spreading, proliferation, extracellular matrix(ECM) mineralization, and collagen secretion of r BMSCs. Besides, the zirconium ions released from different Ti–Zr alloy layers could promote the expressions of osteogenesis related genes, including alkaline phosphatase(ALP), type I collagen(Col-I), osteocalcin(OCN), osteopontin(OPN), and runt-related transcription factor 2(Runx2). The study provides reference for the design of Ti–Zr alloys and the surface modification of titanium. | Bangcheng Yan Ji Tan Donghui Wang Jiajun Qiu Xuanyong Liu | 2020 | Progress in Natural Science:Materials International2020，30，5： | 2 |
| 9 | Effects of glycerol on apoptotic signaling pathways during boar spermatozoa cryopreservation显示文摘 | Changjun Zeng Keyi Tang Lian He Wenpei Peng Li Ding Donghui Fang Yan Zhang | 2014 | Cryobiology2014，，： | 2 |
| 10 | A Globally Convergent Derivative-Free Method for Solving Large-Scale Nonlinear Monotone Equations显示文摘 | YAN Qinrong PENG Xiaozhen LI Donghui | 2010 | Journal of Computational and Applied Mathematics2010，234，3： | 1 |
| 11 | Hyperspectral image unmixing algorithm based on endmember-constrained nonnegative matrix factorization显示文摘 | Yan ZHAO Zhen ZHOU Donghui WANG Yicheng HUANG Minghua YU | 2016 | Frontiers of Optoelectronics2016，9，4： | 1 |
| 12 | A Case Study of Aerosol Characteristics During a Haze Episode Over Beijing显示文摘 | Wanzhi Xu He Chen Donghui Li Fengsheng Zhao Yan Yang | 2013 | Procedia Environmental Sciences2013，，： | 1 |
| 13 | Cloning and sequence analysis of β-actin gene from Aedes albopictus (Diptera: Culicidae)显示文摘Objective: To obtain the complete β-actin gene from Aedes albopictus. Methods: Total RNA was extracted from C6/36 cells. Degenerate primers were designed based on the β-actin sequences of An. gambiae, Ae. aegypti, Cx. pipiens pallens and D. melanogaster. By RT-PCR, the product was amplified, purified, cloned into the pGT vector and sequenced. The β-actin sequence was aligned and phylogenetically analyzed by the BLAST program and the CLUSTAL W program. Results: A sequence of 1132 bp including an open reading frame of 1131 bp was obtained(GenBank DQ657949). The deduced protein had 376 amino acids. Aligned to SWISS-PROT, it exhibited a high level of identity with β-actins from Anopheles, Drosophila and Culex at the amino acid sequence level. Phylogenetic analysis indicated that Ae. albopictus β-actin was much more homologous with invertebrate β-actin than with vertebrate β-actin. Conclusion: The gene may be used as the internal control in the experiments of Ae. albopictus. | Weijie Wang Xiaobang Hu Donghui Zhang Jianhua Jiao Yan Sun Lei M' Changliang Zhu | 2007 | Journal of Nanjing Medical University2007，21，2： | 1 |
| 14 | Investigation of the Potential and Mechanism of Clove for Mitigating Airborne Particulate Matter Emission from Stationary Sources显示文摘植物的障碍(VB ) 有潜力减轻从区域来源射出的空气污染物质,包括喂操作(AFO ) 的集中的动物。然而,机制充分没被调查,从而在实践限制植被系统的应用程序。有可重复、可控制的条件的一个试验性的方法被开发测量微粒事(下午)集中的变化在在风中的 VB 迎风、顺风与扫描电子显微镜( SEM )在叶子掘并且观察积累的下午,因此在减轻从 AFO 射出的下午评估 VB 的能力。分支规模植被,劈开(syzygium aromaticum ) 因为它的叶子是专业之一,被选择因素影响下午分散。结果证明分支规模障碍多孔的媒介有能力防碍气流和还原剂下午,它能被风速度,粒子尺寸部分和表面区域密度影响劈开。而且,劈开元素能适应风和微结构劈开(例如叶子的层次结构) 在 PM 免职上影响了。这些结果显示在这研究开发的方法可以被用来在从静止来源减轻下午评估植被的潜力,并且植被的一些特征能是为探索减少微粒的工程申请的进一步学习的同样仿生的原型。 | Jin Tong Xin Liu Ronaldo Maghirang Kaiqi Wei Linna Liu Chun Wang Yunhai Ma Donghui Chen Hongjia Yan Li Guo | 2017 | Journal of Bionic Engineering2017，14，2： | 1 |
| 15 | Development of a novel chemokine signaling-based multigene signature to predict prognosis and therapeutic response in colorectal cancer显示文摘Colorectal cancer(CRC)is the most lethal gastrointestinal cancer in both males and females worldwide(Sung et al.,2021).Because of the high heterogeneity of tumors,robust prognostic biomarkers are urgently needed in CRC management(Koncina et al.,2020).Chemokine signaling is a well-known pivotal player in immunity,inflammation,and cancer metastasis(Lacalle et al.,2017;Poeta et al.,2019;Do et al.,2020),and multiple genes involved in chemokine signaling have been demonstrated as potential prognostic biomarkers for CRC(Cabrero-De Las Heras and Martinez-Balibrea,2018;Ottaiano et al.,2020;Yu et al.,2020).Therefore,the aim of our study was to develop a chemokine signaling-based multigene signature(CSbMgSig)that could effectively predict overall survival(OS)and therapeutic response for patients with CRC. | Xin QI Donghui YAN Jiachen ZUO Rui WANG Jiajia CHEN | 2021 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2021，22，12： | 1 |
| 16 | Advances in Researches about Immunoregulatory Functions of Dendrobium Sw.显示文摘Based on the researches on immunoregulatory functions of Dendrobium. at home and abroad in recent years,from immune organs,immune cells,and immune molecules,this paper summarized immunoregulatory functions of polysaccharides,glycosides,and alkaloids extracted from Dendrobium. on the body,including reducing the inflammation,inhibiting tumor,and slowing down aging,to provide theoretical references for in-depth researches and development of modern biotechnology for Dendrobium. | Donghui WANG Bei FAN Fang FANG Yan WANG Cencan XING Lijing ZHANG Fengzhong WANG | 2018 | Medicinal Plant2018，9，2： | 0 |
| 17 | Flotation and adsorption of novel Gemini decyl-bishydroxamic acid on bastnaesite:Experiments and density functional theory calculations显示文摘Rare earth element is an important strategic metal,but the supply of high purity rare earth ores is growing slowly,which is in sharp contradiction with the rapidly growing demand.Froth flotation has been confirmed to be an effective method to separate bastnaesite from its gangue minerls.However,the traditional collectors are facing serious problems in flotation separation of minerals,requiring the addition of excess depressant and regulator in the flotation process.Herein,we proposed and synthesized novel Gemini hydroxamic acids Octyl-bishydroxamic acid(OTBHA),Decyl-bishydroxamic acid(DCBHA)and Dodecyl-bishydroxamic acid(DDBHA)as the collectors in bastnaesite-barite flotation system.The effect of different carbon chain lengths on the molecular properties were explored by density functional theory(DFT)calculations.DCBHA possessed a stronger reactivity compared with OCBHA and DDBHA.The flotation results verified the consistency of the computational calculation about the performance prediction of Gemini hydroxamic acids.Compared with OCBHA and DDBHA,DCBHA displayed superior collecting affinity toward bastnaesite,and did not float barite.Zeta potential results showed that the presence of DCBHA increased the potential of bastnaesite,while it had almost no effect on barite,indicating DCBHA had a stronger affinity for bastnaesite.Then,Fourier transform infrared(FTIR)and X-ray photoelectron spectroscopy(XPS)analyses indicated that the adsorption mechanism was due to two hydroxamate groups of DCBHA co-anchored on bastnaesite surface by forming five-membered hydroxamic―(O―O)―Ce complexes.In addition,atomic force microscopy(AFM)clearly observed that DCBHA uniformly aggregated on bastnaesite surface,which increased surface contact angle and improved the hydrophobicity of bastnaesite. | Longhua Xu Chang Liu Jiushuai Deng Donghui Wang Kai Xue Yan Wang Jinping Meng Jiongtian Liu | 2023 | International Journal of Mining Science and Technology2023，33，10： | 0 |
| 18 | Quantitative Evaluation of Leaf Inclination Angle Distribution on Leaf Area Index Retrieval of Coniferous Canopies显示文摘Both leaf inclination angle distribution(LAD)and leaf area index(LAI)dominate optical remote sensing signals.The G-function,which is a function of LAD and remote sensing geometry,is often set to 0.5 in the LAI retrieval of coniferous canopies even though this assumption is only valid for spherical LAD.Large uncertainties are thus introduced.However,because numerous tiny leaves grow on conifers,it is nearly impossible to quantitatively evaluate such uncertainties in LAI retrieval.In this study,we proposed a method to characterize the possible change of G-function of coniferous canopies as well as its effect on LAI retrieval.Specifically,a Multi-Directional Imager(MDI)was developed to capture stereo images of the branches,and the needles were reconstructed.The accuracy of the inclination angles calculated from the reconstructed needles was high.Moreover,we analyzed whether a spherical distribution is a valid assumption for coniferous canopies by calculating the possible range of the G-function from the measured LADs of branches of Larch and Spruce and the true G-functions of other species from some existing inventory data and threedimensional(3D)tree models.Results show that the constant G assumption introduces large errors in LAI retrieval,which could be as large as 53%in the zenithal viewing direction used by spaceborne LiDAR.As a result,accurate LAD estimation is recommended.In the absence of such data,our results show that a viewing zenith angle between 45 and 65 degrees is a good choice,at which the errors of LAI retrieval caused by the spherical assumption will be less than 10%for coniferous canopies. | Guangjian Yan Hailan Jiang Jinghui Luo Xihan Mu Fan Li Jianbo Qi Ronghai Hu Donghui Xie Guoqing Zhou | 2021 | Journal of Remote Sensing2021，，1： | 0 |
| 19 | Detection to Pathogens of Poplar Cankers by Multiplex PCR Technique显示文摘Fungal pathogens causing poplar canker diseases are cosmopolitan in species,and have a wide range of hosts.These pathogens have diverse anamorphs and their morphology overlaps with each other;Their teleomorphs are hardly discovered in nature.Furthermore,the identification of these pathogens is usually limited by the geography,host and taxonomic knowledge.Therefore,a culture-independent method used to identify determine pathogens is expected to be developed for field diagnostics.Through amplifying,sequencing and analyzing multiplex nucleic acid templates and genetic marked target sequences,a multiplex PCR technique has been established and used to directly detect various environmental samples.In this study, four pathogen strains and environmental samples were amplified using fungal universal primers ITS1/ ITS4 and EF1-728F/EF1-986R by general PCR and multiplex PCR.The amplicons were sequenced,and then aligned in GenBank.The result showed that the multiplex PCR was able to successfully amplify the target gene and identify the pathogens from environmental samples in the condition of an annealing temperature of 55.6℃and primers final concentration of 0.2μmol·L-1.The multiplex PCR could amplify the target at the concentration level of approximately lng of genomic DNA.This method would provide a useful tool for the identification of canker pathogens by the multiplex PCR and the high-throughput DNA microarray detection of environmental samples. | Feng Xiaohui Zhang Xingyao Yan Donghui | 2012 | Chinese Forestry Science and Technology2012，11，3： | 0 |
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