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| 1 | Oncofetal antigen glypican-3 as a promising early diagnostic marker for hepatocellular carcinoma显示文摘BACKGROUND:Hepatocellular carcinoma (HCC) is characterized by a multi-cause,multi-stage and multi-focus process of tumor progression.Its prognosis is poor and early diagnosis is of utmost importance.This study was undertaken to investigate the dynamic expression of oncofetal antigen glypican-3 (GPC-3) and GPC-3 mRNA in hepatocarcinogenesis and to explore their early diagnostic value for HCC.METHODS:A hepatoma model was induced in male Sprague-Dawley rats with 0.05% 2-fluorenylacetamide and confirmed by hematoxylin and eosin staining and gamma-glutamyltransferase (GGT) expression.Total RNA was purified and transcribed into cDNA by reverse transcription.Fragments of the GPC-3 gene were amplified by nested RT-PCR,and confirmed by sequencing.GPC-3 was analyzed by immunohistochemistry,Western blotting or ELISA.RESULTS:Positive GPC-3 expression showed as brown granule-like staining localized in the cytoplasm.Histological examination of hepatocytes revealed three morphological stages of granule-like degeneration,atypical hyperplasia (precancerous),and cancer formation,with a progressive increase of liver total RNA and GGT expression.The incidence of liver GPC-3 mRNA and GPC-3,and serum GPC-3 was 100%,100% and 77.8% in the HCC group,100%,100%,and 66.7% in the precancerous group,83.3%,83.3%,and 38.9% in the degeneration group,and no expression in the liver or blood of the control group,respectively.There was a positive correlation between liver GPC-3 mRNA and total RNA level (r=0.475,P<0.05) or liver GPC-3 (r=1.0,P<0.001) or serum GPC-3 (r= 0.994,P<0.001).CONCLUSION:Abnormal oncofetal antigen GPC-3 and GPC-3 mRNA expression in hepatocarcinogenesis may be promising molecular markers for early diagnosis of HCC. | Research Center of Clinical Medicine,Affiliated Hospital of Nantong University,Nantong 226001,China (Yao M,Yao DF,Qiu LW,Wu W,Sai WL,Yang JL and Zhang HJ) Department of Oncology,Yancheng First People’s Hospital,Yancheng 224001,China (Bian YZ) Department of Oncology,Second Affiliated Hospital,Nanjing Medical University,Nanjing 210011,China (Zhang CG) | 2011 | Hepatobiliary & Pancreatic Diseases International2011，10，3： | 51 |
| 2 | Restoration Prospects for Heitutan Degraded Grassland in the Sanjiangyuan显示文摘In many ecosystems ungulates have coexisted with grasslands over long periods of time. However, high densities of grazing animals may change the floristic and structural characteristics of vegetation, reduce biodiversity, and increase soil erosion, potentially triggering abrupt and rapid changes in ecosystem condition. Alternate stable state theory provides a framework for understanding this type of dynamic. In the Sanjiangyuan atop the Qinghai-Tibetan plateau (QTP), grassland degradation has been accompanied by irruptions of native burrowing animals, which has accentuated the loss of ground cover. Severely degraded areas of alpine meadows are referred to as 'Heitutan'. Here, using the framework of alternate stable state theory, we describe the proximate and ultimate drivers of the formation of Heitutan on the QTP, and we assess prospects for recovery, in relation to the degree of biophysical alteration, of these alpine meadows. Effective rehabilitation measures must address the underlying causes of degradation rather than their symptoms. Heitutan degradation is not uni-causal. Rather it reflects different mechanisms operating at different spatio-temporal scales across this vast region. Underlying causes include overly aggressive exploitation of the grasslands (e.g. overgrazing), amplification of grazing and erosion damage by small mammals when outbreaks occur, and/or climate change. Given marked variability in environmental conditions and stressors, restorative efforts must vary across the region. Restoration efforts are likely toyield greatest success if moderately and severely degraded areas are targeted as the first priority in management programmes, before these areas are transformed into extreme Heitutan. | LI Xi-lai PERRY LW George BRIERLEY Gary GAO Jay ZHANG Jing YANG Yuan-wu | 2013 | Journal of Mountain Science2013，10，4： | 23 |
| 3 | Effect of PA-MSHA vaccine on plasma phospholipids metabolic profiling and the ratio of Th2/Thl cells within immune organ of mouse IgA nephropathy显示文摘 | Jia, LW Wang, C Kong, HW Yang, J Li, FL Lv, S Xu, GW | 2007 | 中国生物学文摘2007，21，11： | 19 |
| 4 | Mammalian de- fensins in immunity:more than just microbicidal 显示文摘 | Yang D Biragyn A Kwak LW | 2002 | Trends Immunol2002，23，6： | 1 |
| 5 | Decreased expression of C-erbB-2and CXCR4in breast cancer after primary chemotherapy显示文摘 | Yang SX Loo WT Chow LW | 2012 | J Transl Med2012，，1： | 1 |
| 6 | Open-branched stent graft placement makes total arch replacement easier for acute type a aortic dissection显示文摘 | Chen LW Dai XF Yang GF | 2010 | Ann Thorac Surg2010，89，5： | 1 |
| 7 | Highly efficient KF/Al_2O_3-catalyzed versatile hetero-Michael addition of nitrogen,oxygen,and sulfur nucleophiles to α,β-ethylenic compounds显示文摘 | Yang L Xu LW Xia CG | 2005 | Tetrahedron Lett2005，46，19： | 1 |
| 8 | New Constituents from Hypericum Japonicum显示文摘NewConstituentsfromHypericumJaponicumJaponicumQingLiWU;ShengPingWANG;YongHongLIAO;LiWeiWANG;YuXiuFENG;JunShanYANG;PeiGenXIAO(... | Wu, QL Wang, SP Liao, YH Wang, LW Feng, YX Yang, JS Xiao, PG Hong, SL | 1996 | Chinese Chemical Letters1996，7，11： | 1 |
| 9 | A clinical database of breast cancer patients reveals distinctive clinico- pathological characteristics: a study from central China 显示文摘 | Wang LW Yang GF Chen JM | 2014 | Asian Pac J Cancer Prev2014，15，4： | 1 |
| 10 | Expression of esophageal cancer related gene 4 (ECRG4), a novel tumor suppressor gene,in esophageal canc- er and its inhibitory effect on the tumor growth in vitro and in vivo 显示文摘 | Li LW Yu XY Yang Y | 2009 | Int J Cancer2009，125，7： | 1 |
| 11 | Clinical efficacy of cyclosporin a neoral in the treatment of paediatric lupus nephritis with heavy proteinuria 显示文摘 | Fu LW Yang LY Chen WP | 1998 | Br J Rheumatol1998，37，2： | 1 |
| 12 | Mammalian defensins in immunity:more than just microbicidal显示文摘 | Yang D Biragyn A Kwak LW | | 0，，06： | 1 |
| 13 | Early Postnatal Parathion Exposure in Rats Causes Sex-Selective Cognitive Impairment and Neuro- transmitter Defects Which Emerge in Aging显示文摘 | Levin ED Timofeeva OA Yang LW | 2010 | Behav Brain Res2010，208，： | 1 |
| 14 | Curcumin induces apoptosis in human non-small cell lung cancer NCI-H460 cells through ER stress and caspase cascade-and mito-chondria-dependent pathways显示文摘 | Wu SH Hang LW Yang JS | 2010 | Anticancer Res2010，30，6： | 1 |
| 15 | Mammalian detensins in immunity: more than just microbieidal显示文摘 | Yang D Biragyn A Kwak LW | 2002 | Trends in Immunology2002，23，6： | 1 |
| 16 | Inotodiol inhabits proliferation and induces apoptosis through modulating expression of cyclinE,p27,bcl-2,and bax in human cervical cancer HeLa cells显示文摘 | Zhao LW Zhong XH Yang SY | 2014 | Asian Pac J Cancer Prey2014，15，7： | 1 |
| 17 | Role of endothenous carbon monoxide in hypertension pathagenesis of rats显示文摘 | Ou HS Yang J Dong LW | 1998 | Sheng li xue bao(S0371--0874)1998，50，6： | 1 |
| 18 | Comparative and demographic analysis of orangutan genomes显示文摘 | Locke DP Hillier LW Warren WC Worley KC Nazareth LV Muzny DM Yang SP Wang Z Chinwalla AT Minx P Mitreva M Cook L Delehaunty KD Fronick C Schmidt H Fulton LA Fulton RS Nelson JO Magrini V Pohl C Graves TA Markovic C Cree A Dinh HH Hume J Kovar CL Fowler GR | | 0，，： | 1 |
| 19 | Glaucocalyxin A induces apoptosis in human leukemia HL-60 cells through mitochondria mediated death pathway 显示文摘 | Gao LW Zhang J Yang WH | 2011 | Toxicol In Vitro2011，25，1： | 1 |
| 20 | Correlation of viral factors with cervical cancer in Taiwan 显示文摘 | Yang YY Koh LW Tsai JH | 2004 | J Microbiol Immunol Infect2004，37，5： | 1 |
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