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| 1 | First identification of kdr allele F1534S in VGSC gene and its association with resistance to pyrethroid insecticides in Aedes albopictus populations from Haikou City,Hainan Island,China显示文摘Background:Aedes albopictus is distributed widely in China,as a primary vector of Dengue fever and Chikungunya fever in south of China.Chemical insecticide control is one of the integrated programmes to prevent mosquito-borne diseases.Long-term applications of pyrethroids have resulted in the development of resistance in Ae.albopictus populations in China.However,the susceptibility of Ae.albopictus to pyrethroids in Hainan Island was unclear.Knockdown resistance(kdr),caused by point mutations in the VGSC gene,is one of the mechanisms that confer resistance to DDT and pyrethroids.This study was to investigate the resistance level of Ae.albopictus populations in Haikou City to three pyrethroid insecticides,and elucidate the relationship between the resistant phenotype and kdr mutations.Methods:The Aedes albopictus samples were collected in Xinbu Island(XI),Longtang Town(LT),Shishan Town(ST),Baishamen Park(BP),and Flower Market(FM)from Haikou City,Hainan Island,China.The larval susceptibility to deltamethrin,permethrin and beta-cypermethrin was tested by larval bioassays,and adult susceptibility to deltamethrin and DDT was determined by adult bioassays.The degree of resistance was determined by resistance ratio value(RR50>3)for larvae and by mortality for adult.The kdr alleles at codon 1534 of the VGSC gene were genotyped.The relationship between kdr genotypes and resistant phenotypes was analyzed by Chi-square test.Results:Out of five populations,assessed by larval bioassays,XI was susceptible to deltamethrin and permethrin;LT was susceptible to permethrin and beta-cypermethrin;and ST was susceptible to permithrin.FM and BP both were resistant to all of the three pyrethroids,and FM showed the highest degree of resistance,with RR50 values from 65.17 to 436.36.A total of 493 individuals from the larval bioassays were genotyped for kdr alleles.Five alleles were detected,including two wildtype alleles,TTC(F)(67.04%)and TTT(F)(0.41%),and three mutant alleles,TGC(C)(0.30%),TCC(S)(31.54%)and TTG(L)(0.71%).There was a clear correlation between mutant alleles(or F1534S)and resistant phenotypes(P<0.01).Conclusion:Two novel kdr mutant alleles F1534S and F1534L were detected in the pyrethroid resistant populations of Ae.albopictus in Haikou Hainan,China.For the first time,the mutant F1534S was associated with pyrethroid resistance in Ae.albopictus. | Huiying Chen Kaili Li Xiaohua Wang Xinyan Yang Yi Lin Fang Cai Wenbin Zhong Chunyan Lin Zhongling Lin Yajun Ma | 2016 | Infectious Diseases of Poverty2016，5，1： | 19 |
| 2 | Characterization of an Organ Specific and Pathogen Responsive CC-NBS-LRR Gene from Cotton(Gossypium hirsutum L.)显示文摘Cotton diseases represent a major challenge to cotton growth.Cloning of a cotton pathogen response gene and promoter is of great importance to improve disease resistance.In this study,a | ZHANG Bao-long,NI Wan-chao,YANG Yu-wen,SHEN Xin-lian(Institute of Agro-biotechnology,Jiangsu Academy of Agricultural Sciences,Zhongling Street 50,Nanjing 210014,China) | 2008 | 棉花学报2008，20，S1： | 3 |
| 3 | Event-related potentials during imitated natural reading 显示文摘 | Xie Ying Yang Zhongle | 2002 | International Journal of Psychophysiology2002，45，12： | 1 |
| 4 | Event-related Potentials during Imitated Natural Reading 显示文摘 | Xie Ying Yang Zhongle | 2002 | International Journal of Psychophysiology2002，45，1： | 1 |
| 5 | Environmen- tal friendly anodizing of AZ91D magnesium alloy in alka- line borate-benzoate electrolyte 显示文摘 | Yan Liu Zhongling Wei Fuwei Yang | 2011 | Journal of Alloys and Compounds2011，，509： | 1 |
| 6 | Ultrasound-assisted ionic liquid/ionic liquid-dispersive liquid–liquid microextraction for the determination of sulfonamides in infant formula milk powder using high-performance liquid chromatography显示文摘 | Shiqian Gao Xiao Yang Wei Yu Zhongling Liu Hanqi Zhang | 2012 | Talanta2012，，： | 1 |
| 7 | Event-related potentials during imitated natural reading 显示文摘 | Xie Ying Yang Zhongle | 2002 | International Journal of Psychophysiology2002，45，12： | 1 |
| 8 | Event-related Potentials during Imitated Natural Reading显示文摘 | Ying Xie Zhongle Yang | 2002 | International Journal of Psyehophysiology2002，，45： | 1 |
| 9 | Design, synthesis, and antiviral properties of 2-aryl-1H- benzimidazole-4-carboxamide derivatives显示文摘A series of new benzimidazole derivatives were designed and synthesized.Their chemical structures were testified by 1 H NMR,infrared spectroscopy(IR),mass spectrography(MS),and elemental analysis.Their potent antiviral properties indicated the prospect of new drugs.Compound 13,16,18,19,21,22,and 23 were identified as novel antivirus with much better selective activity and inhibitory activity than the comparable ribavirin against Coxsackie virus B_(3) in VERO cells. | Xianjin LUO ZhonglüZHANG Yutian YANG Fei XUE Naiyun XIU Yuanbin SHE | 2009 | Frontiers of Chemical Science and Engineering2009，3，3： | 0 |
| 10 | Effects of In-video Questions and Feedback on Learning Performance显示文摘The eye-tracking technology was used in this study to investigate the effects of embedded questions and feedback in instructional videos on learning performance and attention allocation and whether an expertise reversal effect existed.The experiment involved 49 learners with high-level prior knowledge and 45 ones with low-level prior knowledge from a university.Meanwhile,they learned instructional videos with no embedded feedback,embedded questions without feedback and embedded questions with feedback.Findings from the experiment showed that the instructional videos with embedded questions but without feedback not only improved the participants’attention but also enhanced their learning performance.Furthermore,there was an expertise reversal effect on the learning performance whereby instructional videos with embedded questions but without feedback improved the learning performance of learners with low-level prior knowledge,but not those with high-level prior knowledge. | XIE Yaohui YANG Jiumin PI Zhongling DAI Chenyan LIU Caixia | 2022 | Frontiers of Education in China2022，17，1： | 0 |
| 11 | Dependence of Long Range Correlation on Width of Rapidity Gap in High Energy Hadron-Hadron Collisions显示文摘The dependence of long range correlation in high energy hadron-hadron collisions on the width of rapidity gap is studied,It is shown that the result from a model with only one particle source does not fit the experimental data.In order to get the experimental dependence of long range correlation on the width of rapidity gap at least three particle sources are needed. | YANG Jie LIU Lianshou YANG Zhongle | 1994 | Chinese Physics Letters1994，11，10： | 0 |
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