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| 1 | Two-substrate vertical deposition for stable colloidal crystal chips显示文摘By combining vertical deposition with micro- molding in capillaries method, we have demonstrated the two-substrate vertical deposition, an alternative and versatile procedure for fabricating high-quality stable colloidal crystal chips. Apparent bright colors, special UV-vis spectra, scan- ning electron microscopy (SEM) and atomic force micros- copy (AFM) images all prove that high-quality colloidal crystal structures are formed in between the two substrates. During the two-substrate vertical deposition for colloidal crystal chips, capillary force and evaporation of the medium are critical to the formation of the colloidal crystals; while the confinement in between two close substrates makes the resulting colloidal crystal chips more stable. Due to the ex- cellent stability, these colloidal crystal chips can be used to construct some composite optical devices via a simpler and more flexible process. Meanwhile, they can also be further used as the templates for ordered multiporous materials. | CHENXin SUNZhiqiang CHENZhimin ZHANGKai YANGBai | 2005 | Chinese Science Bulletin2005，50，8： | 13 |
| 2 | A new three-layer-funnel-shaped esophagogastric anastomosis for surgical treatment of esophageal carcinoma显示文摘AIM: To reduce the incidence of postoperative anastomoticleak, stenosis, gastroesophageal reflux (GER) for patientswith esophageal carcinoma, and to evaluate the conventionalmethod of esophagectomy and esophagogastroplastymodified by a new three-layer-funnel-shaped (TLF)esophagogastric anastomotic suturing technique.METHODS: From January 1997 to October 1999, patientswith clinical stage Ⅰ and Ⅱ (Ⅱa and Ⅱb) esophagealcarcinoma, which met the enrollment criteria, were surgicallytreated by the new method (Group A) and by conventionaloperation (Group B). All the patients were followed at leastfor 6 months. Postoperative outcomes and complicationswere recorded and compared with the conventional methodin the same hospitals and with that reported previously byMcLarty etalin 1997 (Group C).RESULTS: 58 cases with stage Ⅰ and Ⅱ (Ⅱa and Ⅱb)esophageal carcinoma, including 38 males and 20 femalesaged from 34 to 78 (mean age: 57), were surgically treatedby the TLF anastomosis and 64 by conventional method inour hospitals from January 1997 to October 1999. The qualityof swallowing was improved significantly (Wilcoxon W=2 142,P=0.0 001) 2 to 3 months after the new operation in GroupA. Only one patient had a blind anastomatic fistula diagnosedby barium swallow test 2 months but healed up 3 weekslater. Postoperative complications occurred in 25 (43 %)patients, anastomotic stenosis in 8 (14 %), and GER in 13(22 %). The incidences of postoperative anastomotic leak,stenosis and GER were significantly decreased by the TLFanastomosis method compared with that of conventionalmethods (x2=6.566, P =0.038; x2=10.214, P= 0.006;x2=21.265, P=0.000).CONCLUSION: The new three-layer-funnel-shapedesophagogastric anastomosis (TLFEGA) hasmore advantagesto reduce postoperative complications of anastomotic leak,stricture and GER. | Han-LeiDan YangBai HuiMeng JieZhang YongZhang Ya-LiZhang Zhen-ShuZhang Dian-YuanZhou | 2003 | World Journal of Gastroenterology2003，9，1： | 10 |
| 3 | Pathogenicty and immune prophylaxis of cag pathogenicity island gene knockout homogenic mutants显示文摘AIM: To clarify the role of cag pathogenicity island (cagPAI) of Helicobacter pylori (H pylori) in the pathogenicity and immune prophylaxis of H pylori infection. METHODS: Three pairs of H pylori including 3 strains of cagPAI positive wildtype bacteria and their cagPAI knockout homogenic mutants were utilized. H pylori binding to the gastric epithelial cells was analyzed by flow cytometry assays. Apoptosis of gastric epithelial cells induced by H pylori was determined by ELISA assay. Prophylaxis effect of the wildtype and mutant strains was compared by immunization with the sonicate of the bacteria into mice model. RESULTS: No difference was found in the apoptasis between cagPAI positive and knockout H pylori strains in respective of the ability in the binding to gastric epithelial cells as well as the induction of apoptosis. Both types of the bacteria were able to protect the mice from the infection of H pylori after immunization, with no difference between them regarding to the protection rate as well as the stimulation of the proliferation of splenocytes of the mice. CONCLUSION: The role of cagPAI in the pathogenicity and prophylaxis of Hpyloriinfection remains to be cleared. | Huan-JianLin JingXue YangBai Ji-DeWang Ya-LiZhang Dian-YuanZhou | 2004 | World Journal of Gastroenterology2004，10，22： | 7 |
| 4 | Construction of attenuated Salmonella typhimurium Strain expressing Helicobacter pyloriconservative region of adhesin antigen and its immunogenicity显示文摘AIM: To construct a non-resistant and attenuated Salmonella typhimurium ( S. typhimurium) strain which expresses conservative region of adhesion AB of Helicobacter pylori(Hpylon) and evaluate its immunogenicity.METHODS: The AB gene amplified by PCR was inserted into the expression vector pYA248 containing asd gene and through two transformations introduced into the delta Cya1 delta Crp1 delta Asd attenuated Salmonella typhimurium strain, constructing balanced lethal attenuated Salmonella typhimurium strains X4072 (pYA248-AB). Bridged ELISA method was used to measure the expression of AB antigen in sonicate and culture supematant. According to the method described by Meacock, stability of the recombinant was evaluated. Semi-lethal capacity test was used to evaluate the safety of recombinant. The immunogenicity of recombinant was evaluated with animal experiments.RESULTS: The attenuated S. typhimurium X4072 (pYA248-AB) which expresses AB was successfully constructed.Furthermore, bridged ELISA assay showed that the content of AB in recombinant X4072 (pYA248- AB) culture supematant was higher than that was in thallus lytic liquor. And after recombinant X4072 (pYA248- AB) was cultured for 100 generations without selection pressure, bhe entire recombinant bacteria selected randomly could grow, and the AB antigen was defected positive by ELISA. The growth curve of the recombinant bacteria showed that the growth states of X4072 (pYA248) and X4072 (pYA248-AB) were basically consistent. The survival rate of C57BL/6 was still 100%, at 30 d after mice taking X4072 (pYA248-AB) 1.0×l0^10 cfu orally. Oral immunization of mice with X4072 (pYA248-AB) induced a specific immune response.CONCLUSION: In vitro recombinant plasmid appears to be stable and experiments on animals showed that the recombinant strains were safe and immunogenic in vitro,which providing a new live oral vaccine candidate for protection and care of H pylori infection. | YangBai Ya-LiZhang Ji-DeWang Zhao-ShanZhang Dian-YuanZhou | 2004 | World Journal of Gastroenterology2004，10，17： | 6 |
| 5 | Preparation of CdTe nanocrystal-polymer composite microspheres in aqueous solution by dispersing method显示文摘Highly fluorescent CdTe nanocrystals were synthesized in aqueous solution, and then processible CdTe nanocrystal-polymer composites were fabricated by coating the aqueous nanocrystals with copolymers of styrene and octadecyl-p-vinyl-benzyldimethylammonium chloride (SOV- DAC) directly. A dichloromethane solution of CdTe nano- crystal-polymer composites was dispersed in the aqueous solution of poly (vinyl alcohol) (PVA) generating highly fluo- rescent microspheres. Experimental parameters such as the concentration of nanocrystal-polymer composites, the con- centration of PVA, and stirring speed which had important effect on the preparation of the microspheres were investi- gated in detail with fluorescent microscope characterization. | LIMinjie WANGChunlei HANKun YANGBai | 2005 | Chinese Science Bulletin2005，50，7： | 5 |
| 6 | Expression of Helicobacter pylori Hsp60 protein and its immunogenicity显示文摘AIM: To express Hsp60 protein of H pylori by a constructed vector and to evaluate its immunogenicity.METHODS: Hsp60 DNA was amplified by PCR and inserted into the prokaryotie expression vector pET-22b (+), which was transformed into BL21 (DE3) E. coli strain to express recombinant protein. Immunogenicity of expressed Hsp60 protein was evaluated with animal experiments.RESULTS: DNA sequence analysis showed Hsp60 DNA was the same as GenBank's research. Hsp60 recombinant protein accounted for 27.2 % of the total bacterial protein,and could be recognized by the serum from H pylori infected patients and Balb/c mice immunized with Hsp60 itself.CONCLUSION: Hsp60 recombinant protein might become a potential vaccine for controlling and treating H pylori infection. | YangBai Liang-RenLi Ji-DeWang YeChen Jian-FengJin Zhao-ShanZhang, Dian-YuanZhou Ya-LiZhang | 2003 | World Journal of Gastroenterology2003，9，12： | 5 |
| 7 | Cloning and expression and immunogenicity of Helicobacter pylori BabA_2 gene显示文摘AIM: To construct a recombinant strain which expresses BabA of Helicobacter pylori(H pylori) and to study the immunogenicity of BabA.METHODS: BabA2 DNA was amplified by PCR and inserted into the prokaryotie expression vector pET-22b (+) and expressed in the BL21 (DE3) E.coli strain. Furthermore,BabA immunogenicity was studied by animal test.RESULTS: DNA sequence analysis showed the sequence of BabA2 DNA was the same as the one published by GenBank.The BabA recombinant protein accounted for 34.8% of the total bacterial protein. The serum from H pylori infected patients and Balb/c miced immunized with BabA itself could recognize rBabA.CONCLUSION: BabA recombinant protein may be an potential vaccine for control and treatment of Hpyloriinfection. | YangBai Ya-LiZhang YeChen Jian-FengJin Zhao-ShanZhang Dian-YuanZhou | 2004 | World Journal of Gastroenterology2004，10，17： | 4 |
| 8 | Study on the Relationship Between Helicobacter pylori in the Dental Plaque and the Occurrence of Dental Caries or Oral Hygiene Index显示文摘 | YingLiu HuanjianLin YangBai XiaoshuQin XinZheng YongSun YaliZhang | 2008 | Helicobacter2008，，4： | 2 |
| 9 | Recombinant Helicobacter pylori catalase显示文摘AIM: To construct a recombinant strain which highly expresses catalase of Helicobacter pylori(H.pylori) and assay the activity of H. pylori catalase.METHODS: The catalase DNA was amplified from H. pylori chromosomal DNA with PCR techniques and inserted into the prokaryotie expression vector pET-22b (+), and then was transformed into the BL21 (DE3) E. coli strain which expressed catalase recombinant protein. The activity of H.pylori catalase was assayed by the Beers & Sizers.RESULTS: DNA sequence analysis showed that the sequence of catalase DNA was the same as GenBank's research. The catalase recombinant protein amounted to 24.4 % of the total bacterial protein after induced with IPTG for 3 hours at 37 ℃ and the activity of H. pylori catalase was high in the BL21 (DE3) E. coli strain.CONCLUSION: A clone expressing high activity H. pylori catalase is obtained, laying a good foundation for further studies. | YangBai Ya-LiZhang Jian-FengJin Ji-DeWang Zhao-ShanZhang Dian-YuanZhou | 2003 | World Journal of Gastroenterology2003，9，5： | 2 |
| 10 | Endoscopic papillary balloon dilatation versus endoscopic sphincterotomy in the treatment for choledocholithiasis: A meta‐analysis显示文摘 | YangyangLiu PeizhuSu SihengLin KunXiao PingyanChen ShengliAn FachaoZhi YangBai | 2012 | Journal of Gastroenterology and Hepatology2012，，3： | 1 |
| 11 | Benzophenoxazinium-based red-Emitting chemosensor for zinc ions in biological media 显示文摘 | Yang X B YangBai X Ge J F | 2011 | Organic Letters2011，13，10： | 1 |
| 12 | CADM1/TSLC1 inactivation by promoter hypermethylation is a frequent event in colorectal carcinogenesis and correlates with late stages of the disease显示文摘 | KequanChen GuanghaiWang LiangPeng SideLiu XiangshengFu YuZhou HaishengYu AimingLi JingLi ShaohengZhang YangBai YaliZhang | 2010 | Int. J. Cancer2010，，： | 1 |
| 13 | Synthesis and properties of water-soluble oligo-(p-phenylkene vinylene)显示文摘A water-soluble oligo(p-phenylkene vinylene) [4,4′-(1,4-phenylene dithenylene)-bis-(N-methyl pyridinium iodide)] was synthesized by Witting reaction. The oligomer was characterized by NMR, elemental analysis, UV-visible spectrum, surface photovoltage spectrum and fluorescence spectrum. | TANGJun YUXiaoqiang LINWeiran SUNJingzhi YANGBai | 2003 | Chinese Science Bulletin2003，48，20： | 1 |
| 14 | COVID-19-associated gastrointestinal and liver injury:clinical features and potential mechanisms显示文摘Coronavirus disease-2019(COVID-19)is caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2).The infection is spreading globally and poses a huge threat to human health.Besides common respiratory symptoms,some patients with COVID-19 experience gastrointestinal symptoms,such as diarrhea,nausea,vomiting,and loss of appetite.SARS-CoV-2 might infect the gastrointestinal tract through its viral receptor angiotensin-converting enzyme 2(ACE2)and there is increasing evidence of a possible fecal–oral transmission route.In addition,there exist multiple abnormalities in liver enzymes.COVID-19-related liver injury may be due to drug-induced liver injury,systemic inflammatory reaction,and hypoxia–ischemia reperfusion injury.The direct toxic attack of SARS-CoV-2 on the liver is still questionable.This review highlights the manifestations and potential mechanisms of gastrointestinal and hepatic injuries in COVID-19 to raise awareness of digestive system injury in COVID-19. | Peijie Zhong Jing Xu Dong Yang Yue Shen Lu Wang Yun Feng Chunling Du Yuanlin Song Chaomin Wu Xianglin Hu Yangbai Sun | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 1 |
| 15 | TNF-α inhibits fibrosis and migration of fibroblasts in hypertrophic scar by miR-141-3p显示文摘Hypertrophic scar is a dermal fibroproliferative disease characterized by increased collagen bundles[1].A variety of factors participate in its formation,including inflammation,genetics,growth factors,and cytokines[2-5]. | Renpeng Zhou Chuandong Wang Dongze Lv Yangbai Sun Yimin Liang | 2021 | Acta Biochimica et Biophysica Sinica2021，53，8： | 1 |
| 16 | Partial Linear Models for Longitudinal Data Based on Quadratic Inference Functions显示文摘 | YANGBAI ZHONGYIZHU WING K.FUNG | 2008 | Scandinavian Journal of Statistics2008，，1： | 1 |
| 17 | New Synthesis Approach and Magnetic Properties of Hot Pressed/Hot Deformed Nanocomposite Magnets显示文摘The new process of preparation of hotpressed/hot deformed nanocomposite magnet was studied. The possibility of making high anisotropic bulk magnets with high magnetic performances was investi-gated. The relation between the density, magnetic properties and the parameter of process was studied. The material used is magnetic powder composed of two phases, | YangBai SunGuangfei ChenJufang QiaoYi WangHaojie HuGuohui | 2005 | Journal of Rare Earths2005，23，2： | 0 |
| 18 | Expression of Helicobacter pyloriAlpA protein and its immunogenicity显示文摘AIM: To construct a recombinant strain which expresses adhesin AlpA of Helicobacter pylori (H pylori ) and to study the immunogenicity of adhesin AlpA.METHODS: Gene Ab, which was amplified from Hpylori chromosomal DNA by PCR technique, was sequenced and the biological information was analyzed, and inserted into the NcoⅠ and NotⅠ restriction fragments of the expression vector pET-22b(+) using T4 DNA ligase. The resulting plasmid pET-AlpA was transformed into competent E.coli BL21(DE3) cells using ampicillin resistance for selection. Recombinant strains were incubated in 5mL LB with 100μg/mL ampicillin overnight at 37℃. Sonication of BL21(DE3)pET-22b(+)/AlpA was analyzed by Western blot to detect AlpA immunogenicity.RESULTS: The gene encoding AlpA protein was amplified by PCR with chromosomal DNA of H pylori Sydney strain (SS1) as templates. It revealed that AlpA DNA fragmentamplified by PCR had approximately 1 500 nucleotides,compatible with the previous reports. The recombinantplasmid pET-22b(+)/AB was successfully constructed. DNAsequencing showed one open reading frame with thelength of 588 bp. It encoded seven conservative regionsthat showed good antigenicity and hydrophobicity by Parkerand Welling method. Furthermore, INTERNET EXPASY,NNPREDICT and ISREC predicted that it was a porin-likestructure consisting of [3-pleated sheets that were embeddedin the outer membrane. BLAST analyzed 836 767 proteinsequences and found that the similar sequences were allbelonging to HpyloriOMP sequences. SDS-PAGE and scananalysis showed that the molecular weight of AB was22.5 ku and recombinant protein amounted to 29% of thetotal bacterial protein, among which dissolved expressionamounted to 21.9% of sonicated supernatant. The rABpurity amounted to 96% through affinity chromatography.Western blot analysis of rAB confirmed that it could bespecially recognized by serum form rabbit immunized withAlpA and Hpylori infected.CONCLUSION: Adhesin AlpA recombinant protein may be a potential vaccine for control and treatment of H pylori infection. | JingXue YangBai YeChen Ji-DeWang Zhao-ShanZhang Ya-LiZhang Dian-YuanZhou | 2005 | World Journal of Gastroenterology2005，11，15： | 0 |
| 19 | Acute diarrhea during army field exercise in southern China显示文摘AIM: During emergency period, infectious diseases can be a major threat to military forces. During field training in southern China, diarrhea is the main cause of nonbattle injury. To evaluate the causes of and risk factors for diarrhea in emergency period, we collected clinical and epidemiological data from the People's Liberation Army (PLA) during fieldtraining in southern China. METHODS: From September 25 to October 2 1997, 2636 military personnel were investigated. Fecal sample cultures for lapactic pathogens were obtained from 103 military personnel with diarrhea. In addition, a questionnaire was administered to 103 cases and 206 controls to evaluate the association between illness and potential risk factors. At the same time,another questionnaire of 1:4 case-case control was administered to 22 severe cases (each severe case paired 4 mild cases). RESULTS: The training troop's diarrhea incidence rate was significantly higher than that of garrison. The diarrhea incidence rate of officers was significantly lower than that of soldiers. A lapactic pathogen was identified in 63.1% (65/103) of the troops with diarrhea. Enterotoxigenic Escherichia coli (35.0%) and plesiomona shigelloides (16.5%) were the most common bacterial pathogens. All bacterial isolates were sensitive to norfloxacin and ceftazidine. However, almost all of them were resistant to sulfamethoxazole, trimethoprim-sulfamethoxazole,oxytetracycline, doxycycline, furazolidone, ampicillin and cloromycetin to a different degree. Risk factors associated with diarrhea includediidrinking raw water, eating outside,contacting diarrhea patients, lacking sanitation, depression,lacking sleep, which were established by multiple-factor logistic regression analysis. In addition, the unit incidence rate was associated with the density of flies and the average daily boiled water available by regression and discriminate analysis. CONCLUSION: A series of risk factors are associated with the incidence rate of diarrhea. Our results may provide a useful basis for prevention and cure of diarrhea in emergency period of PLA. | YangBai Ying-ChunDai Jian-DongLi JunNie QingChen HongWang Yong-YuRui Ya-LiZhang Shou-YiYu | 2004 | World Journal of Gastroenterology2004，10，1： | 0 |
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