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| 1 | Japanese Encephalitis Virus NS1' Protein Antagonizes Interferon Beta Production显示文摘Japanese encephalitis virus(JEV) is a mosquito-borne virus and the major cause of viral encephalitis in Asia. NS1', a52-amino acid C-terminal extension of NS1, is generated with a-1 programmed ribosomal frameshift and is only present in members of the Japanese encephalitis serogroup of flaviviruses. Previous studies demonstrated that NS1' plays a vital role in virulence, but the mechanism is unclear. In this study, an NS1' defected(rG66A) virus was generated. We found that rG66A virus was less virulent than its parent virus(pSA14) in wild-type mice. However, similar mortality caused by the two viruses was observed in an IFNAR knockout mouse model. Moreover, we found that rG66A virus induced a greater type Ⅰ interferon(IFN) response than that by pSA14, and JEV NS1' significantly inhibited the production of IFN-b and IFN-stimulated genes. Taken together, our results reveal that NS1' plays a vital role in blocking type I IFN production to help JEV evade antiviral immunity and benefit viral replication. | Dengyuan Zhou Fan Jia Qiuyan Li Luping Zhang Zheng Chen Zikai Zhao Min Cui Yunfeng Song Huanchun Chen Shengbo Cao Jing Ye | 2018 | Virologica Sinica2018，33，6： | 3 |
| 2 | PD1^+CCR2^+CD8^+T Cells Infiltrate the Central Nervous System during Acute Japanese Encephalitis Virus Infection显示文摘Japanese encephalitis(JE)is a viral encephalitis disease caused by Japanese encephalitis virus(JEV)infection.Uncontrolled inflammatory responses in the central nervous system(CNS)are a hallmark of severe JE.Although the CCR2-CCL2 axis is important for monocytes trafficking during JEV infection,little is known about its role in CNS trafficking of CD8^+T cells.Here,we characterized a mouse model of JEV infection,induced via intravenous injection(i.v.)and delineated the chemokines and infiltrating peripheral immune cells in the brains of infected mice.The CNS expression of chemokines,Ccl2,Ccl3,and Ccl5,and their receptors,Ccr2 or Ccr5.was significantly up-regulated after JEV infection and was associated with the degree of JE pathogenesis.Moreover,JEV infection resulted in the migration of a large number of CD8^+T cells into the CNS.In the brains of JEV-infected mice,infiltrating CD8^+T cells expressed CCR2 and CCR5 and were found to comprise mainly effector T cells(CD44^+CD62L_).JEV infection dramatically enhanced the expression of programmed death 1(PD-1)on infiltrating CD8^+T cells in the brain,as compared to that on peripheral CD8^+T cells in the spleen.This effect was more pronounced on infiltrating CCR2^+CD8^+T cells than on CCR2-CD8^+T cells.In conclusion,we identified a new subset of CD8^+T cells(PD1^+CCR2^+CD8^+T cells)present in the CNS of mice during acute JEV infection.These CD8^+T cells might play a role in JE pathogenesis. | Fang Zhang Linlin Qi Tong Li Xiaojing Li Dan Yang Shengbo Cao Jing Ye Bin Wei | 2019 | Virologica Sinica2019，34，5： | 2 |
| 3 | Inhibition of porcine circovirus type 1 and type 2 production in PK-15 cells by small interfering RNAs targeting the Rep gene显示文摘 | Minxuan Sun Xueqin Liu Shengbo Cao Qigai He Rui Zhou Jing Ye Yaoming Li Huanchun Chen | 2007 | Veterinary Microbiology2007，，1： | 1 |
| 4 | Effects of incident energy and angle on carbon cluster ions implantation on silicon substrate:a molecular dynamics study显示文摘Carbon cluster ion implantation is an important technique in fabricating functional devices at micro/nanoscale. In this work, a numerical model is constructed for implantation and implemented with a cuttingedge molecular dynamics method. A series of simulations with varying incident energies and incident angles is performed for incidence on silicon substrate and correlated effects are compared in detail. Meanwhile, the behavior of the cluster during implantation is also examined under elevated temperatures. By mapping the nanoscopic morphology with variable parameters, numerical formalism is proposed to explain the different impacts on phrase transition and surface pattern formation. Particularly, implantation efficiency(IE) is computed and further used to evaluate the performance of the overall process. The calculated results could be properly adopted as the theoretical basis for designing nano-structures and adjusting devices' properties. | Ye Wei Shengbo Sang Bing Zhou Xiao Deng Jing Chai Jianlong Ji Yang Ge Yuanliang Huo Wendong Zhang | 2017 | Journal of Semiconductors2017，38，9： | 0 |
| 5 | A COMPACT AND HANDHELD SURFACE PENETRATING RADAR FOR THE DETECTION OF REINFORCED CONCRETE STRUCTURES显示文摘Surface Penetrating Radar (SPR) is a recently developed technology for non-destructive testing. It can be used to image and interpret the inner structure of the reinforced concrete. This paper gives the details about a compact and handheld SPR developed recently for reinforced concrete structure detection. The center operation frequency of the radar is 1.6 GHz. Not only it has fast acquisition ability, but also it can display the testing result on the LCD screen in real-time. The testing results show that the radar has a penetrating range of more than 30 cm, and a lateral resolution better than 5 cm. The performance validates that the radar can meet the application requirements for reinforced concrete structure detection. | Zhou Bin Ye Shengbo Xia Xinfan Shao Jinjin Liu Lihua Fang Guangyou | 2013 | Journal of Electronics(China)2013，30，4： | 0 |
| 6 | Epidemiological trends of mosquito-borne viral diseases in Pakistan显示文摘Globally,arboviruses are public health problems.Pakistan has seen a fast-paced increase in mosquito-borne Flavivirus diseases such as dengue because of deforestation,climate change,urbanization,poor sanitation and natural disasters.The magnitude and distribution of these diseases are poorly understood due to the lack of a competitive nationwide surveillance system.In dengue-endemic countries,the recent epidemics of chikungunya(CHIKV)and human West Nile virus(WNV)have created panic among the public and are thought to provoke an outbreak of Zika virus(ZIKV)in Pakistan.Recently,hospital-based surveillance has indicated the presence of Japanese encephalitis virus(JEV),which is deeply concerned by developing countries such as Pakistan.The situation could become more devastating because of poorly developed diagnostic infrastructure.To date,no licensed vaccine has been used in Pakistan,and preventive measures are mainly based on vector control.This review provides comprehensive information concerning the association of risk factors with disease occurrence,epidemiological trends,and prediction of the spread of mosquito-borne diseases,attention to new threats of ZIKV,and future perspectives by benchmarking global health policies. | Muhammad Imran Jing Ye Muhammad K.Saleemi Iqra Shaheen Ali Zohaib Zheng Chen Shengbo Cao | 2022 | Animal Diseases2022，2，2： | 0 |
| 7 | Isolation and phylogenetic analysis of feline calicivirus strains from various region of China显示文摘Feline calicivirus(FCV)is an important feline pathogen mainly causing upper respiratory tract disease,conjunctivitis,and stomatitis,and it is classifed into genotype I and genotype II.To investigate the prevalence and molecular characteristics of FCV,this study collected 337 cat swab samples from animal hospitals in diferent regions of China from 2019 to 2021.The positive detection rate of FCV was 29.9%(101/337)by RT-PCR.Statistical analysis showed that FCV prevalence was signifcantly associated with living environment(p=0.0004),age(p=0.031)and clinical symptoms(p=0.00),but not with sex(p=0.092)and breed(p=0.171).The 26 strains of FCV were isolated using F81 cells.Phylogenetic analysis showed that 10 isolates belonged to genotype I,and 16 isolates belonged to genotype II.These 26 isolates were highly genetically diverse,of which HB7 isolate had three same virulence-related amino acid loci with VSD strains.Potential loci distinguishing diferent genotypes were identifed from 26 isolates,suggesting the genetic relationship between diferent genotypes.In addition,selection pressure analysis based on capsid protein of 26 isolates revealed that the protein is under diversifying selection.This study reveals the genetic diversity of FCV and provides a reference for the screening of vaccine candidate strains and the development of vaccines with better cross-protection efects. | Longlong Cao Qiuyan Li Kaituo Shi Liting Wei Hehao Ouyang Zijun Ye Wenguang Du Jiawen Ye Xiaochen Hui Jiakang Li Shengbo Cao Dengyuan Zhou | 2022 | Animal Diseases2022，2，3： | 0 |
| 8 | Oxygen‑Coordinated Single Mn Sites for Efficient Electrocatalytic Nitrate Reduction to Ammonia显示文摘Electrocatalytic nitrate reduction reaction has attracted increasing attention due to its goal of low carbon emission and environmental protection.Here,we report an efficient NitRR catalyst composed of single Mn sites with atomically dispersed oxygen(O)coordination on bacterial cellulose-converted graphitic carbon(Mn-O-C).Evidence of the atomically dispersed Mn-(O-C_(2))_(4)moieties embedding in the exposed basal plane of carbon surface is confirmed by X-ray absorption spectroscopy.As a result,the as-synthesized Mn-O-C catalyst exhibits superior NitRR activity with an NH_(3)yield rate(RNH_(3))of 1476.9±62.6μg h^(−1)cm^(−2)at−0.7 V(vs.reversible hydrogen electrode,RHE)and a faradaic efficiency(FE)of 89.0±3.8%at−0.5 V(vs.RHE)under ambient conditions.Further,when evaluated with a practical flow cell,Mn-O-C shows a high RNH_(3)of 3706.7±552.0μg h^(−1)cm^(−2)at a current density of 100 mA cm−2,2.5 times of that in the H cell.The in situ FT-IR and Raman spectroscopic studies combined with theoretical calculations indicate that the Mn-(O-C_(2))_(4)sites not only effectively inhibit the competitive hydrogen evolution reaction,but also greatly promote the adsorption and activation of nitrate(NO_(3)^(−)),thus boosting both the FE and selectivity of NH_(3)over Mn-(O-C_(2))_(4)sites. | Shengbo Zhang Yuankang Zha Yixing Ye Ke Li Yue Lin Lirong Zheng Guozhong Wang Yunxia Zhang Huajie Yin Tongfei Shi Haimin Zhang | 2024 | Nano-Micro Letters2024，16，1： | 0 |
| 9 | Ferroptosis contributes to JEV-induced neuronal damage and neuroinflammation显示文摘Ferroptosis is a newly discovered prototype of programmed cell death (PCD) driven by iron-dependent phospholipid peroxidation accumulation, and it has been linked to numerous organ injuries and degenerative pathologies. Although studies have shown that a variety of cell death processes contribute to JEV-induced neuroinflammation and neuronal injury, there is currently limited research on the specific involvement of ferroptosis. In this study, we explored the neuronal ferroptosis induced by JEV infection in vitro and in vivo. Our results indicated that JEV infection induces neuronal ferroptosis through inhibiting the function of the antioxidant system mediated by glutathione (GSH)/glutathione peroxidase 4 (GPX4), as well as by promoting lipid peroxidation mediated by yes-associated protein 1 (YAP1)/long-chain acyl-CoA synthetase 4 (ACSL4). Further analyses revealed that JEV E and prM proteins function as agonists, inducing ferroptosis. Moreover, we found that treatment with a ferroptosis inhibitor in JEV-infected mice reduces the viral titers and inflammation in the mouse brains, ultimately improving the survival rate of infected mice. In conclusion, our study unveils a critical role of ferroptosis in the pathogenesis of JEV, providing new ideas for the prevention and treatment of viral encephalitis. | Wenjing Zhu Qi Li Yong Yin Huanchun Chen Youhui Si Bibo Zhu Shengbo Cao Zikai Zhao Jing Ye | 2024 | Virologica Sinica2024，39，1： | 0 |
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