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| 1 | Propofol differentially induces unconsciousness and respiratory depression through distinct interactions between GABAa receptor and GABAergic neuron in corresponding nuclei显示文摘Propofol is the most commonly used intravenous anesthetic worldwide.It can induce loss of consciousness prior to the occurrence of severe respiratory suppression,which is also a pharmacodynamic feature of all general anesthetics.However,the neural mechanisms underlying this natural phenomenon are controversial and highly related to patient safety.In the present study,we demonstrated that the pharmacodynamic effects of propofol(50 and 100 μM)on suppression of consciousness-related excitatory postsynaptic currents in the medial prefrontal cortex(mPFC)and centromedian nucleus of the thalamus(CMT)were lower than those in the kernel respiratory rhythmogenesis nucleus pre-Botzinger complex(PrBo).Furthermore,we unexpectedly found that the GABAa receptor β3 subunit is the key target for propofol's action and that it is mutually and exclusively expressed in GABAergic neurons.It is also more abundant in the mPFC and CMT,but mainly co-localized with GABAergic neurons in the PrBo.As a result,the differentiated expression pattern should mediate more neuron suppression through the activation of GABAergic neurons in the mPFC and CMT at low doses of propofol(50 μM).However,PrBo GABAergic neurons were only activated by propofol at a high dose(100 μM).These results highlight the detailed pharmacodynamic effects of propofol on consciousness-related and respiration-related nuclei and provide the distinct interaction mechanism between the β3 subunit and GABAergic neurons in mediating the suppression of consciousness compared to the inhibition of respiration. | Junli Jiang Yingfu Jiao Po Gao Wen Yin Wei Zhou Yunchun Zhang Yanjun Liu Daxiang Wen Yuan Wang Liang Zhou Tian Yu Weifeng Yu | 2021 | Acta Biochimica et Biophysica Sinica2021，53，8： | 11 |
| 2 | Activation of anterior thalamic reticular nucleus GABAergic neurons promotes arousal from propofol anesthesia in mice显示文摘Propofol is widely used for the induction and maintenance of anesthesia,which causes a rapid loss of consciousness.However,the mechanisms underlying the hypnosis effect of propofol are still not fully understood.The thalamic reticular nucleus(TRN)is crucial for regulating wakefulness,sleep rhythm generation,and sleep stability,while the role of TRN in the process of propofol-induced anesthesia is still unknown.Here,we investigated the function of the anterior TRN in propofol general anesthesia.Our results demonstrated that the neural activity of anterior TRN is suppressed during propofol anesthesia,whereas it is robustly activated from anesthesia by recording the calcium signals using fiber photometry technology.The results showed that the activation of anterior TRN neurons by chemogenetic and optogenetic methods shortens the emergency time without changing the induction time.Conversely,chemogenetic or optogenetic inhibition of the TRN neurons leads to a delay in the recovery time.Our study showed that anterior TRN is crucial for behavioral arousal without affecting the induction time of propofol anesthesia. | Yanjun Liu Bing Chen Yirong Cai Yuan Han Ying Xia Nanqi Li Bingqian Fan Tianjie Yuan Junli Jiang Po Gao Weifeng Yu Yingfu Jiao Wenxian Li | 2021 | Acta Biochimica et Biophysica Sinica2021，53，7： | 3 |
| 3 | THEORY AND APPLICATION OF πBRIDGE HYDRAULIC RESISTANCE NETWORKS显示文摘1INTRODUCTIONTherearetwokindsofhydraulicnetworks,i.e.4armsbridgehydraulicresistancenetworkandhalfbridgehydraulicresistancene... | Yanping, Hu Jianhua, Wei Yingfu, Guo Genmao, Wu Deshun, Liu | 1999 | 中国有色金属学会会刊:英文版1999，9，1： | 3 |
| 4 | Hippocampus is more vulnerable to neural damages induced by repeated sevoflurane exposure in the second trimester than other brain areas显示文摘During the rapidly developing and sensitive period of the central nervous system (CNS), a harmful stimulus may have serious consequences. The effect of anesthetic exposure on the development of the offspring’s CNS during pregnancy is still unclear and has been widely concerned. In the present study, we compared the susceptibility of the hippocampus with those of other brain regions in offsprings when the mother mice were exposed to repeated sevoflurane. We found that other than affecting motor sensation, emotion, or social behavior of offspring mice, repeated sevoflurane exposure induced significant memory deficiency. Compared with other brain regions, the hippocampus, which is the key component of the brain serving for learning and memory, was more vulnerable to repeated sevoflurane exposure. We also found that repeated sevoflurane exposure to mother mice could inhibit the axon development of hippocampal neurons. We also predicted that N6-methyladenosine modification of mRNA might play an essential role in the vulnerability of the hippocampus to sevoflurane, while the underlying cellular mechanism needs to be explored in the future. Our study may provide a new perspective for studying the mechanism of hippocampus-specific injury induced by sevoflurane exposure. | Bing Chen Yanjun Liu Yirong Cai Dan Tang Saihong Xu Po Gao Weifeng Yu Yingfu Jiao Wenxian Li | 2020 | Acta Biochimica et Biophysica Sinica2020，52，8： | 3 |
| 5 | Dexmedetomidine maintains blood-brain barrier integrity by inhibiting Drp1-related endothelial mitochondrial dysfunction in ischemic stroke显示文摘Stroke is the second leading cause of death and long-term disability worldwide,which lacks effective treatment.Perioperative stroke is associated with much higher rates of mortality and disability.The neuroprotective role of dexmedetomidine(Dex),a highly selective agonist of alpha2-adrenergic receptor,has been reported in a stroke rat model,and it was found that pretreatment of Dex before stroke could alleviate blood–brain barrier(BBB)breakdown.However,the underlying mechanisms are still unknown.As the brain endothelial cells are the main constituents of BBB and in high demand of energy,mitochondrial function of endothelial cells plays an important role in the maintenance of BBB.Given that dynamin-related protein 1(Drp1)is a protein mediating mitochondrial fission,with mitochondrial fusion that balances mitochondrial morphology and ensures mitochondria function,the present study was designed to investigate the possible role of Drp1 in endothelial cells involved in the neuroprotective effects of Dex in ischemic stroke.Our results showed that preconditioning with Dex reduced infarction volume,alleviated brain water content and BBB damage,and improved neurological scores in middle cerebral artery occlusion rats.Meanwhile,Dex enhanced cell activity and decreased cell apoptosis in oxygen-glucose deprivation human brain microvascular endothelial cells in vitro.These protective effects of Dex were correlated with the mitochondrial morphology integrality of endothelial cells,mediated by increased phosphorylation of serine 637 in Drp1,and could be reversed byα2-adrenergic receptor antagonist Yohimbine and AMP-activated protein kinase inhibitor Compound C.These findings suggest new molecular pathways involved in the neuroprotective effects of Dex in ischemic stroke.As Dex is routinely used as a sedative drug clinically,our findings provide molecular evidence that it has perioperative neuroprotection from ischemic stroke. | Wei Zhou Yunchun Zhang Yingfu Jiao Wen Yin Haiping Dong Saihong Xu Dan Tang Junli Jiang Jianlin Shao Zhenhong Wang Weifeng Yu | 2021 | Acta Biochimica et Biophysica Sinica2021，53，9： | 2 |
| 6 | CC chemokine ligand 2 (CCL2)enhances TTX-sensitive sodium channel activity of primary afferent neurons in the complete Freud adjuvant-induced inflammatory pain model显示文摘CC chemokine ligand 2 (CCL2)has been implicated in pathological pain,but the mechanism underlying the pronociceptive effect of CCL2 is not fully understood.Voltage-gated sodium (Nav)channels are important determinants of the excitability of sensory neurons.Hence we tested the hypothesis that CCL2 contributes to inflammatory pain via modulating Nay channel activity of primary afferent neurons. Chronic inflammatory pain was induced in rats by intraplantar injection of the complete Freud adjuvant (CFA)to one of the hind paws.Control rats received intraplantar injection of equal volume of saline.A significant increase of CCL2 mRNA and CCL2 receptor (CCR2)protein expression was detected in the ipsilateral dorsal root ganglion (DRG)in CFA-treated rats.lntrapiantar injection of CCL2 protein in the control rats had minimal effect on the paw withdrawal threshold (PWT)in response to mechanical stimulation.However,in CFA-treated rats,intraplantar CCL2 led to an increase in pain responses.Patchclamp recording of acutely dissociated DRG neurons revealed that CCL2 had minimum effect on the excitability of sensory neurons from control rats.However,CCL2 directly depolarized a large proportion of small to medium-sized sensory neurons from CFA-treated rats.In addition,CCL2 was found to enhance whole-cell TTX-sensitive sodium currents without significantly affecting the TTX-resistant sodium currents and the potassium currents.These results are in agreement with previous reports concerning the involvement of CCL2-CCR2 signaling in inflammatory hyperalgesia and further indicate that enhanced TTX-sensitive channel activity may partly underlie the pronociceptive effects of CCL2. | Ping Luo Jiayun Shao Yingfu Jiao Weifeng Yu Weifang Rong | 2018 | Acta Biochimica et Biophysica Sinica2018，50，12： | 2 |
| 7 | The Age of the Original Silurian Badangshan Formation and its Ductile Deformation in the Northern Margin of North China Craton: New Evidence from Zircon SHRIMP U-Pb Ages显示文摘Objective Many previous researches have documented the tectonic evolution of the northern margin of North China Craton.However,whether the age of original Silurian Badangshan Formation in the study area belongs to the Archean,Silurian or Devonian remains controversial,and the ductile deformation time of this formation also lack | CUI Yuliang Qu Hongjie CHEN Yingfu WANG Sen MENG Xianfeng SONG Dianlan ZHANG Wenyong WANG Huan | 2017 | Acta Geologica Sinica(English Edition)2017，91，6： | 2 |
| 8 | Rapid detection of orf virus by loop-mediated isothermal amplification based on the DNA polymerase gene显示文摘 | Jida Li Deguang Song Wenqi He Yingfu Bao Rongguang Lu Gaoli Su Gaili Wang Huijun Lu Kui Zhao Feng Gao | 2013 | Archives of Virology2013，，4： | 2 |
| 9 | Two-stage photo-biological production of hydrogen by marine green alga Platyrnonas subcordi formis 显示文摘 | GUAN Yingfu DENG Maieun YU Xingju | 2004 | Biochem Eng J2004，19，1： | 1 |
| 10 | Significant enhancement of photobiological H2 evolution by carbonylcyanide m-chlorophenylhydrazone in the marine green alga Platymonas subcordiformis显示文摘 | GUAN Yingfu ZHANG Wei DENG Maicun | 2004 | Biotechnol Lett2004，26，13： | 1 |
| 11 | A fuzzy CBR technique for generating product ideas显示文摘 | Wu Muhcherng Lo Yingfu Hsu Shanghwa | 2008 | Expert System with Applications2008，34，1： | 1 |
| 12 | Hypothalamic paraventricular nucleus neurons activated by estrogen GPER1 receptors promote anti-inflammation effects in the early stage of colitis显示文摘The hypothalamus-pituitary-adrenal(HPA)axis is known to mediate gut-brain interaction,and the pathological inflammatory process in the intestine can induce HPA axis involved'fight or flight response to suppress or facilitate intestinal inflammation.Hypothalamic paraventricular nucleus(PVN)neurons are responsible for controlling the HPA axis activity,but their exact role in modulating intestinal inflammation remains unclear.In this study,we used the dextran sulfate sodium(DSS)-induced mice colitis model,gene editing,and RNA interference to determine the effects of PVN neurons on intestinal inflammation.We found that at the early stage(third day)after DSS treatment,there was a mild inflammation in the colorectal area and an increased neuron activatio n in the PVN but not in the adjace nt area.At the same time,~80% of activated PVN n eurons also expressed novel estrogen GPER1 receptor.The colitis noticeably worsened in GPER1-knockout mice and local PVN GPER1-knockdown mice.These results indicated that PVN GPER1 positive neurons potentially have a protective function during the early stages of DSS-induced colitis,and this may be a mechanism by which the central nervous system attempts to suppress intestinal inflammation to achieve self-protection. | Tao Jiang Ruoxi Wang Wen Yin Yuxi Zhou Dexu Kong Saihong Xu Po Gao Weifeng Yu Yingfu Jiao Daxiang Wen | 2019 | Acta Biochimica et Biophysica Sinica2019，51，12： | 1 |
| 13 | DNA-enhanced peroxidase activity of a DNA aptamer-hemin complex显示文摘 | Paola Travascio Yingfu Li Dipankar Sen | 1998 | Chemistry & Biology1998，，9： | 1 |
| 14 | The dynamic running law study on driving system of hydraulic winder 显示文摘 | Peng Youduo Liu Deshu Guo Yingfu | 2002 | Journal of Coal science &Engineering2002，8，1： | 1 |
| 15 | Free fatty acid receptor 2 promotes cardiomyocyte hypertrophy by activating STAT3 and GATA4显示文摘Free fatty acids(FFAs)play important roles in cardiovascular disease.Studies have shown that it is an important way for FAs to exert biological effects through their own receptors besides directly participating biochemical reaction in body.Free fatty acid receptor 2(FFA2)can be activated by short-chain FAs and is involved in inflammatory reactions and lipid accumulation.Since the known pathological changes caused by FFA2 are also implicated in cardiac hypertrophy,we hypothesized that FFA2 might be pathogenic in cardiac hypertrophy.This paper showed that FFA2 expression significantly increased in cardiac hypertrophy in vivo and in vitro.FFA2 agonist 4-CMTB or TUG-1375 promoted the expression of the hypertrophy markers ANF and BNP and increased cell surface area in vitro,which was further strengthened by FFA2 overexpression,suggesting that FFA2 might contribute to cardiomyocyte hypertrophy.Furthermore,4-CMTB treatment or FFA2 overexpression combined with 4-CMTB treatment elevated the phosphorylation and transcriptional activity of GATA4 and STAT3,which were inhibited by an ERK1/2 inhibitor,and GATA4 and STAT3 knockdown inhibited the elevation of hypertrophy biomarkers in cardiomyocytes treated with 4-CMTB.Taken together,these data indicate that FFA2 can enhance cardiomyocyte hypertrophy by activating STAT3 and GATA4 via ERK1/2,providing a potential new target for therapy. | Hui Gao Kunming Tian Xiaojun Feng Mengqing Yan Chen Gao Yisheng Jiang Chenhao Zhu Huzhe Zhu Xueping Liu Yingfu Peng | 2022 | Food Science and Human Wellness2022，11，2： | 1 |
| 16 | A fuzzy CBR technique for generating product ideas 显示文摘 | Wu Muheherng Lo Yingfu Hsu Shanghwa | 2008 | Expert Systems with Applications2008，34，1： | 1 |
| 17 | Nucleic acid aptamers and enzymes as sensors显示文摘 | Navani Naveen K Li Yingfu | 2006 | Current Opinion in Chemical Biology2006，10，3： | 1 |
| 18 | Identification of metastasis associated proteins in human lung squamous carcinoma using two-dimensional difference gel electrophoresis and laser capture microdissection显示文摘 | Huixin Yao Zhiqiang Zhang Zhiqiang Xiao Yongheng Chen Cui li Pengfei Zhang Meixiang Li Yingfu Liu Yongjun Guan Yanhui Yu Zhuchu Chen | 2008 | Lung Cancer2008，，1： | 1 |
| 19 | In vitro selection of structure-switching signaling aptamers显示文摘 | Nutiu R Li Yingfu | 2005 | Angew Chem2005，117，： | 1 |
| 20 | New cathodes in aluminum reduction cells显示文摘 | Naixiang-Feng Yingfu Tian Jianping Peng | 2010 | Light Metals2010，，： | 1 |
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