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| 1 | Assessing the numbers of SNPs needed to establish molecular IDs and characterize the genetic diversityof soybean cultivars derived from Tokachi nagaha显示文摘The development of a core set of SNP molecular markers that could be widely used in soybean genetic research would greatly facilitate research into the genetic diversity of soybean.We conducted an analysis of Tokachi nagaha and 137 of its descendant soybean cultivars using 4044 SNP markers with the goal of determining the appropriate number of single-nucleotide polymorphisms(SNPs)needed to construct unambiguous molecular IDs and characterize genetic diversity based on a genetic distance matrix correlation method.When the number of SNPs was held constant,the number of accession pairs that could be distinguished increased as the polymorphism informative content(PIC)value of the SNPs increased.A core panel of 20 selected SNPs from 11 linkage groups with a mean PIC value of 0.3703 and a range of 0.3640–0.3749 was able to identify almost all of the accession pairs in our study[9445 pairs(99.92%)].The eight accession pairs that could not be identified with this core SNP set all originated from the same province and some of them had the same parental cultivars.The molecular IDs of the 138 accessions were constructed using the core 20 SNPs.It is known that both the number of SNPs and PIC values should be considered when SNPs are selected for use in the analysis of genetic diversity.In this study,when the PIC value was 0.3460,the correlation coefficient between the genetic distance matrices associated with a panel of 200 SNPs and the total population was>0.800,indicating satisfactory correlation.Our high-accuracy,high-resolution core SNP panel for germplasm fingerprinting and our findings about assessing genetic diversity will likely markedly improve the management and utilization efficiency of soybean germplasm resources. | Zhangxiong Liu Jun Li Xuhong Fan Nang Myint Phyu Sin Htwe Shuming Wang Wen Huang Jiyu Yang Lili Xing Lijun Chen Yinghui Li Rongxia Guan Ruzhen Chang Dechun Wang Lijuan Qiu | 2017 | The Crop Journal2017，5，4： | 10 |
| 2 | Development and utilization of a new chemically-induced soybean library with a high mutation density显示文摘Mutagenized populations have provided important materials for introducing variation and identifying gene function in plants. In this study, an ethyl methanesulfonate(EMS)-induced soybean(Glycine max) population,consisting of 21,600 independent M_2 lines, was developed.Over 1,000 M_(4(5))families, with diverse abnormal phenotypes for seed composition, seed shape, plant morphology and maturity that are stably expressed across different environments and generations were identified. Phenotypic analysis of the population led to the identification of a yellow pigmentation mutant, gyl, that displayed significantly decreased chlorophyll(Chl) content and abnormal chloroplast development. Sequence analysis showed that gyl is allelic to Minn Gold, where a different single nucleotide polymorphism variation in the Mg-chelatase subunit gene(ChlI1a) results in golden yellow leaves. A cleaved amplified polymorphic sequence marker was developed and may be applied to marker-assisted selection for the golden yellow phenotype in soybean breeding. We show that the newly developed soybean EMS mutant population has potential for functional genomics research and genetic improvement in soybean. | Zhongfeng Li Lingxue Jiang Yansong Ma Zhongyan Wei Huilong Hong Zhangxiong Liu Jinhui Lei Ying Liu Rongxia Guan Yong Guo Longguo Jin Lijuan Zhang Yinghui Li Yulong Ren Wei He Ming Liu Nang Myint Phyu Sin Htwe Lin Liu Bingfu Guo Jian Song Bing Tan Guifeng Liu Maiquan Li Xianli Zhang Bo Liu Xuehui Shi Sining Han Sunan Hua Fulai Zhou Lili Yu Yanfei Li Shuang Wang Jun Wang Ruzhen Chang Lijuan Qiu | 2017 | Journal of Integrative Plant Biology2017，59，1： | 6 |
| 3 | Molecular characterization of GmNHX2,a Na^+/H^+ antiporter gene homolog from soybean,and its heterologous expression to improve salt tolerance in Arabidopsis显示文摘Na+/H+ antiporters have been well documented to enhance plant salt tolerance by regulating cellular ion homeostasis. Here, a putative Na+/H+ antiporter gene homolog GmNHX2 from soybean was cloned and predicted to encode a protein of 534 amino acids with 10 putative transmembrane domains. GmNHX2 was expressed in all soybean plant tissues but enriched in roots and its expression was induced by NaCl and polyethylene glycol (PEG) treatments. GmNHX2 exhibits greater sequence similarity with LeNHX2 and AtNHX6 than that of AtNHX1 and AtSOS1. Although phylogenetic analysis clustered GmNHX2 with organellar (tonoplast and vesicles) antiporters, the GmNHX2-EGFP (enhanced green fluorescent protein) fusion protein was possibly localized in the plasma membrane or organelle membrane of transgenic plant cells. Furthermore, transgenic Arabidopsis plants expressing GmNHX2 were more tolerant to high NaCl concentrations during germination and seedling stages when compared with wild-type plants. These results suggest that GmNHX2 is a membrane Na+/H+ antiporter and may function to regulate ion homeostasis under salt stress. | ZHOU GuoAn GUAN RongXia LI YingHui CHANG RuZhen QIU LiJuan | 2009 | Chinese Science Bulletin2009，54，19： | 4 |
| 4 | Genetic contribution of foreign germplasm to elite Chinese soybean (Glycine max) cultivars revealed by SSR markers显示文摘Simple sequence repeats (SSR) marker analysis, combined with pedigree analysis, was car- ried out to trace the genetic constitutes of Suinong 14 and Hefeng 25 in assessing the contribution of for- eign germplasm to those elite cultivars. The overall goal is to accumulate information for further effective utilization of foreign germplasm in Chinese soybean breeding programs. SSR clustering results indicated that the genetic base of elite Chinese soybean culti- vars, including Suinong 14 and Hefeng 25, were broadened and enhanced via introduction of Amsoy from the U.S. and Shishengchangye from Japan. The pedigree analysis showed a very high coefficient of parentage between Shishengchangye and Hefeng 25, between Shishengchangye and Suinong 14, and between Amsoy and Suinong 14. The genetic simi- larity between Suinong 14 and Hefeng 25 was 60.58%. Among 20 linkage groups, more genomic regions were transferred from Hefeng 25 to Suinong 14 in linkage group (LG) I, L and C2 than other LGs. Among the unique alleles of foreign parents, five specific loci in Amsoy were transferred to Suinong 14, and three loci from Shishengchangye to Suinong 14. Some SSR loci were proved to be correlated with phenotypes: two loci for seed size introgressed from Shishengchangye to Suinong 14, and one locus forprotein content from Amsoy to Suinong 14. These results indicate that the two foreign parents might have important contributions in the development of Suinong 14 and Hefeng 25. | QIN Jun CHEN Weiyuan GUAN Rongxia JIANG Chengxi LI Yinghui FU Yashu LIU Zhangxiong ZHANG Mengchen CHANG Ruzhen QIU Lijuan | 2006 | Chinese Science Bulletin2006，51，9： | 4 |
| 5 | Identification of a novel seed size associated locus SW9-1 in soybean显示文摘Seed size is one of the vital traits determining seed appearance, quality, and yield. Untangling the genetic mechanisms regulating soybean 100-seed weight (100-SW), seed length and seed width across environments may provide a theoretical basis for improving seed yield. However, there are few reports related to QTL mapping of 100-SW across multiple ecological regions. In this study, 21 loci associated with seed size traits were identified using a genome-wide association of 5361 single nucleotide polymorphisms (SNPs) across three ecoregions in China, which could explain 8.12%–14.25% of the phenotypic variance respectively. A new locus, named as SW9-1 on chromosome 9 that explained 10.05%–10.93% of the seed weight variance was found significantly related to seed size traits, and was not previously reported. The selection effect analysis showed that SW9-1 locus has a relatively high phenotypic effect (13.67) on 100-SW, with a greater contribution by the accessions with bigger seeds (3.69) than the accessions with small seeds (1.66). Increases in seed weight were accompanied by increases in the frequency of SW9-1T allele, with >90% of the bred varieties with a 100-SW >30 g carrying SW9-1T. Analysis of SW9-1 allelic variation in additional soybean accessions showed that SW9-1T allele accounting for 13.83% of the wild accessions, while in 46.55% and 51.57% of the landraces and bred accessions, respectively, this results indicating that the SW9-1 locus has been subjected to artificial selection during the early stages of soybean breeding, especially the utilization of SW9-1T in edamame for big seed. These results suggest that SW9-1 is a novel and reliable locus associated with seed size traits, and might have an important implication for increasing soybean seed weight in molecular design breeding. Cloning this locus in future may provide new insights into the genetic mechanisms underlying soybean seed size traits. | Jiajia Li Jinghui Zhao Yinghui Li Yali Gao Sunan Hua Muhammad Nadeem Genlou Sun Wenming Zhang Jinfeng Hou Xiaobo Wang Lijuan Qiu | 2019 | The Crop Journal2019，7，4： | 4 |
| 6 | Establishment of the integrated applied core collection and its comparison with mini core collection in soybean(Glycine max)显示文摘The concept of core collection(CC) provides a new way of management and utilization of plant germplasm resources. In this study, an integrated applied core collection(IACC) of soybean was developed based on evaluation data for desirable agronomic and nutritional traits of available soybean germplasm resources including accessions with cold tolerance, drought tolerance, salt tolerance, soybean cyst nematode resistance, soybean mosaic virus resistance, high protein content, and high fat content. The newly formed collection encompasses accessions with high genetic diversity and desirable agronomic traits. The genetic diversity of the newly formed IACC was compared with that of the established mini core collection(MCC) of soybean with the aid of simple sequence repeat(SSR) markers and phenotypic traits. The results showed that at the molecular level, soybean IACC harbored a similar level of genetic diversity as the established MCC, and that at the phenotypic level the IACC encompasses more accessions with desirable traits than does the established MCC. The development of soybean IACC lays a foundation for breeding projects to meet different objectives in different eco-regions. | Yong Guo Yinghui Li Huilong Hong Li-Juan Qiu | 2014 | The Crop Journal2014，2，1： | 3 |
| 7 | Increased prediction value of biomarker combinations for the conversion of mild cognitive impairment to Alzheimer's dementia显示文摘Background:Progression of mild cognitive impairment(MCI)to Alzheimer's disease(AD)dementia can be predicted by clinical features and a combination of biomarkers may increase the predictive power.In the present study,we investigated whether the combination of olfactory function and plasma neuronal-derived exosome(NDE)Aβ1-42 can best predict progression to AD dementia.Methods:Eighty-seven MCI patients were enrolled and received cognitive assessment at 2-year and 3-year follow-up to reevaluate cognition.In the meanwhile,80 healthy controls and 88 AD dementia patients were enrolled at baseline as well to evaluate the diagnose value in cross-section.Olfactory function was evaluated with the sniffin sticks(SS-16)and Aβ142 levels in NDES were determined by ELISA.Logistic regression was performed to evaluate the risk factors for cognitive decline in MCI at 2-year and 3-year revisits.Results:In the cross cohort,lower SS-16 scores and higher AB142 levels in NDES were found in MCI and AD dementia compared to healthy controls.For the longitudinal set,8 MCI individuals developed AD dementia within 2 years,and 16 MCI individuals developed AD dementia within 3 years.The two-parameter combination of SS-16 scores and Aβ1-42 level in NDEs showed better prediction in the conversion of MCI to AD dementia at 2-year and 3-year revisits.Moreover,after a 3-year follow-up,SS-16 scores also significantly predicted the conversion to AD dementia,where lower scores were associated with a 10-fold increased risk of developing AD dementia(p=0.006).Similarly,higher AB1-42 levels in NDES in patients with MCI increased the risk of developing AD dementia by 8.5-fold(p=0.002).Conclusion:A combination of two biomarkers NDE AB1-42 and SS-16 predicted the conversion of MCI to AD dementia more accurately.These findings have critical implications for understanding the pathophysiology of AD dementia and for developing preventative treatments for cognitive decline. | Aonan Zhao Yuanyuan Li Yi Yan Yinghui Qiu Binyin Li Wei Xu Ying Wang Jun Liu Yulei Deng | 2020 | Translational Neurodegeneration2020，9，3： | 2 |
| 8 | Cloning and sequence diversity analysis of GmHs1 pro-1 in Chinese domesticated and wild soybeans显示文摘 | Cuiping Yuan Guoan Zhou Yinghui Li Kejing Wang Zhi Wang Xianghua Li Ruzhen Chang Lijuan Qiu | 2008 | Molecular Breeding2008，，4： | 1 |
| 9 | The Main Diseases and Control Measures of Panax notoginseng(Burk) F. H. Chen in Wuzhou City显示文摘The main symptoms of root rot,anthracnose,blight and damping-off of Panax notoginseng( Burk) F. H. Chen are introduced in the paper,and the corresponding control measures are elaborated from the aspects of agricultural management measures,seed disinfection,seedbed treatment and chemical control. | Chen Yinghui Liu Zhuowu Qiu Weihua Tan Xinxing Li Jinfeng | 2014 | Plant Diseases and Pests2014，5，2： | 1 |
| 10 | QTL mapping of qSCN3-1 for resistance to soybean cyst nematode in soybean line Zhongpin 03-5373显示文摘Soybean cyst nematode(SCN,Heterodera glycines Ichinohe)is one of the most economically destructive pathogens.The soybean line Zhongpin03-5373(ZP),which combines resistance genes from several donors,is highly resistant to SCN race 3(SCN3).In our previous study,two QTL(rhg1 and GmSNAP11)were identified in a population of recombinant inbred lines derived from a cross between ZP and the susceptible parent Zhonghuang 13.The two QTL explained around one-third of the resistance,suggesting the presence of further QTL contributing to SCN resistance.In the present study,we used an improved version of the geneticmap comprising the previously applied 1062 molecular markers and 47 newly developed InDel(insertion-deletion)markers.The improved map revealed a novel locus contributing to SCN3 resistance:qSCN3-1,flanked by InDelmarker InDel1-7 and SNPmarker Map-0047,explained 4.55%of the phenotypic variance for resistance to SCN3 and was not involved in digenic epistatic interaction with rhg1 and GmSNAP11.Haplotypes of Map-0047_CAPS(a CAPS marker developed for Map-0047)and InDel1-7 were significantly associated with SCN3 resistance in a panel of 209 resistant and susceptible accessions.Using further allele-combination analysis for three functional markers representing three cloned resistance genes(rhg1,Rhg4,andGmSNAP11)and twomarkers flanking qSCN3-1,we found that adding the resistance allele of qSCN3-1 greatly increased soybean resistance to SCN,even in diverse genetic backgrounds.The qSCN3-1 locus will be useful for marker-assisted polygene pyramid breeding and should be targeted for the future identification of candidate genes. | Lei Yang Yu Tian Yulin Liu Jochen C.Reif Yinghui Li Lijuan Qiu | 2021 | The Crop Journal2021，9，2： | 0 |
| 11 | Identification of cancer stem cells: from leukemia to solid cancers显示文摘Cancer stem cells(CSCs)are widely consid-ered to be a small cell population in leukemia and many solid cancers with the properties including self-renewal and differentiation to non-tumorigenic cancer cells.Identification and isolation of CSCs significantly depend on the special surface markers of CSCs.Aberrant gene expression and signal transduction contribute to malig-nancies of CSCs,which result in cancer initiation,progression and recurrence.The inefficient therapy of cancers is mainly attributed to the failure of elimination of the malignant CSCs.However,CSCs have not been detected in all cancers and hierarchical organization of tumors might challenge cancer stem cell models.Addi-tionally,opinions about the validity of the CSC hypothesis,the biological properties of CSCs,and the relevance of CSCs to cancer therapy differ widely.In this review,we discuss the debate of cancer stem cell model,the parameters by which CSCs can or cannot be defined,and the advances in the therapy of CSCs. | Yinghui HUANG Xiaoxue QIU Ji-Long CHEN | 2010 | Frontiers in Biology2010，5，5： | 0 |
| 12 | The prevalence of deleterious mutations during the domestication and improvement of soybean显示文摘Soybean(Glycine max L.)is a protein and oil crop grown worldwide.Its fitness may be reduced by deleterious mutations,whose identification and purging is desirable for crop breeding.In the published whole-genome re-sequenced data of 2214 soybean accessions,including 221 wild soybean,1132 landrace cultivars and 861 improved soybean lines,we identified 115,275 deleterious single-nucleotide polymorphisms(SNPs).Numbers of deleterious alleles increased from wild soybeans to landraces and decreased from landraces to modern improved lines.Genes in selective-sweep regions showed fewer deleterious mutations than the remaining genes.Deleterious mutations explained 4.3%-48%more phenotypic variation than randomly selected SNPs for resistance to soybean cyst nematode race 2(SCN2),soybean cyst nematode race 3(SCN3)and soybean mosaic virus race 3(SMV3).These findings illustrate how mutation load has shifted during soybean domestication,expansion and improvement and provide candidate sites for breeding out deleterious mutations in soybean by genome editing and/or conventional breeding focused on the selection of progeny with fewer deleterious alleles. | Shichao Sun Yumin Wang He Wei David E.Hufnagel Ya Wang Shiyu Guo Yinghui Li Li Wang Li-juan Qiu | 2023 | The Crop Journal2023，11，2： | 0 |
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