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| 1 | Isolation and phylogenetic assignation of actinomycetes in the marine sediments from the Arctic Ocean显示文摘Actinomycetes in five marine sediments collected from the Arctic Ocean at depths of 43 to 3 050 m were cultivated using a variety of media. A total of 61 actinomycete colonies with substrate mycelia only were observed, and no colonies with aerial mycelia were observed under aerobic conditions at 15 ℃. From these colonies, 28 were selected to represent different morphological types.Denaturing gradient gel electrophoresis(DGGE) was used to check the purity of isolates and select representatives for subsequent sequencing. Phylogentic analyses based on nearly full-length 16S ribosomal RNA gene (rDNA) sequences indicated that the actinomycetes isolated were accommodated within genus Rhodococcus of family Nocardiaceae, genus Dietzia of family Dietziaceae, genera Janibacter and Terrabacter of family Instrasporangiaceae and genera Kocuria and Arthrobacter of family Micrococcaceae. One of the strains(P27-24) from the deep-sea sediment at depth of 3 050 m was found to be identical in 16S rDNA sequence(1474/1474)with the radiation-resistant Kocuria rosea ATCC 187T isolated from air. More than half of the isolates showed the similarities ranging from 99.5% to 99.9% in 16S rDNA sequence to dibenzofran-degrading, butyl 2-ethylhexanoate-hydrolysising and nitrile-metabolizing actinomycetes. All the strains isolated were psychrotolerant bacteria and grew better on the media prepared with natural seawater than on the media prepared with deionized water. Three of them (Dietzia sp. P27-10, Rhodococcus sp. S11-3 and Rhodococcus sp.P11-5) had an obligate growth requirement for salt, confirming that these strains are indigenous marine actinomycetes. | YU Yong LI Huirong ZENG Yinxin CHEN Bo | 2005 | Acta Oceanologica Sinica2005，24，6： | 8 |
| 2 | Inhibitory effect of Salicornia europaea on the marine alga Skeletonema costatum显示文摘Exploiting the negative biochemical interference between plants and algal species has been suggested as a method to control harmful algal blooms.In this work,we investigated the inhibitory effect of the salt marsh halophyte Salicornia europaea against the marine alga Skeletonema costatum.S.europaea suppressed the growth of S.costatum in a nutrient-sufficient co-culture system,indicating that the inhibition of algal growth was because of the phytotoxic effect of S.europaea,rather than nutrient competition.We tested aqueous and organic extracts from S.europaea roots against S.costatum.The organic extracts inhibited growth and affected the cell size and chlorophyll a content of S.costatum in a dose-dependent manner.Among the three tested organic extracts,the methanol extract had the greatest effects on S.costatum,followed by butanol extract,and then the chloroform extract.Two flavonoids,rutin and quercetin-3-D-glucoside,were identified in the methanol extract by high performance liquid chromatography.The concentration of rutin was much higher than that of quercetin-3-D-glucoside.In an algal bioassay,rutin inhibited the growth of S.costatum and the inhibitory effect increased with increasing rutin concentration and with decreasing initial algal density.Therefore,we concluded that S.europaea negatively affects the growth of S.costatum,and that rutin,a metabolite of S.europaea,may play a role in this inhibitory effect. | JIANG Dan HUANG LingFeng LIN YongQing NIE LingLing LV SuLian KUANG TingYun LI YinXin | 2012 | Science China(Life Sciences)2012，55，6： | 8 |
| 3 | Molecular genetic diversity of bacteria in the bottom section of arctic sea ice from the Canada Basin显示文摘PCR-DGGE approach was used to analyze bacterial diversity in the bottom section of seven arctic sea ice samples colleted from the Canada Basin. Thirty-two 16S rDNA sequences were obtained from prominent DGGE bands. The closest relatives of these sequences are found to be those of cultivated or uncultured bacteria from antarctic or arctic sea ice. Phylogenetic analysis clustered these sequences or phylotypes within α- proteobacteria, γ-proteobacteria and CFB (cytophaga-flexibacter-bacteroides) group. Sequences belonging to γ-proteobacteria were dominant and members of the CFB group were highly abundant. It was suggested that the CFB group was the representative of the bottom section of sea ice samples. | LI Huirong YU Yong CHEN Bo ZENG Yinxin REN Daming | 2005 | Acta Oceanologica Sinica2005，24，6： | 6 |
| 4 | Interaction of Restin with transcription factors显示文摘Restin, a member of melanoma-associated antigen superfamily gene, was first cloned from differentiated leukemia cell induced by all trans-retinoic acid, and was able to inhibit cell proliferation, but the molecular mechanism was not clear. Since Restin was localized in cell nucleus, and its homolog member, Necdin (neuronal growth suppressor factor), could interact with transcription factors p53 and E2F1, we proposed that Restin might also function as Necdin through interacting with some transcription factors. In this study, transcription factors p53, AP1, ATFs and E2Fs were cloned and used in the mammalian two-hybrid system to identify their in- teraction with Restin. The results showed that only ATF3 had a strong interaction with Restin. It is interesting to know that ATF3 was an important transcription factor for G1 cell cycle initiation in physiological stress response. It was possible that the inhibition of cell proliferation by Restin might be related with the inhibition of ATF3 activity. | WU Yousheng LU Fan QI Yinxin WANG Ruihua ZHANG Jian LU Zifan ZHAO Zhongliang | 2005 | Science China(Life Sciences)2005，48，3： | 5 |
| 5 | Isolation and characteristics of one marine psychrotrophic cellulase-generating bacterium Ar/w/b/75°/10/5 from Chuckchi Sea,Arctic显示文摘Microorganisms living in polar zones play an important part as the potential source of organic activity materials with low temperature characteristics in the biotechnological applications. A psychrotrophic bacterium (strain Ar/w/b/75°/10/5), producing cellulase at low temperatures during late-exponential and early-stationary phases of cell growth, was isolated from sea ice-covered surface water in Chuckchi Sea, Arctic. This bacterium, with rod cells, was Gram-negative, slightly halophilic. Colony growing on agar plate was in black. Optimum growth temperature was 15℃. No cell growth was observed at 35℃ or above. Optimum salt concentration for cell growth was between 2 and 3 % of sodium chloride in media. Maximal cellulase activity was detected at a temperature of 35℃ and pH8. Cellulase was irreversibly inactivated when incubated at 55℃ within 30 min. Enzyme can be kept stable at the temperature no higher than 25℃. Of special interest was that this bacterium produced various extracellular enzymes including cellulase, amylase, agar hydrolase and protease, at low or moderate temperature conditions, which is certainly of it potential value for applications. | Zeng Yinxin(曾胤新) Chen Bo(陈波) | 2002 | Chinese Journal of Polar Science2002，13，2： | 4 |
| 6 | Phylogenetic diversity of dimethylsulfoniopropionate-dependent demethylase gene dmdA in distantly related bacteria isolated from Arctic and Antarctic marine environments显示文摘Dimethylsulfoniopropionate(DMSP) is mainly produced by marine phytoplankton as an osmolyte, antioxidant,predator deterrent, or cryoprotectant. DMSP is also an important carbon and sulfur source for marine bacteria.Bacteria may metabolize DMSP via the demethylation pathway involving the DMSP demethylase gene(dmdA) or the cleavage pathway involving several different DMSP lyase genes. Most DMSP released into seawater is degraded by bacteria via demethylation. To test a hypothesis that the high gene frequency of dmdA among major marine taxa results in part from horizontal gene transfer(HGT) events, a total of thirty-one bacterial strains were isolated from Arctic Kongsfjorden seawater in this study. Analysis of 16S rRNA gene sequences showed that,except for strains BSw22118, BSw22131 and BSw22132 belonging to the genera Colwellia, Pseudomonas and Glaciecola, respectively, all bacteria fell into the genus Pseudoalteromonas. DmdA genes were detected in five distantly related bacterial strains, including four Arctic strains(Pseudoalteromonas sp. BSw22112, Colwellia sp.BSw22118, Pseudomonas sp. BSw22131 and Glaciecola sp. BSw22132) and one Antarctic strain(Roseicitreum antarcticum ZS2–28). Their dmdA genes showed significant similarities(97.7%–98.3%) to that of Ruegeria pomeroyi DSS–3, which was originally isolated from temperate coastal seawater. In addition, the sequence of the gene transfer agent(GTA) capsid protein gene(g5) detected in Antarctic strain ZS2–28 exhibited a genetically closely related to that of Ruegeria pomeroyi DSS–3. Among the five tested strains, only Pseudomonas sp. BSw22131 could grow using DMSP as the sole carbon source. The results of this study support the hypothesis of HGT for dmdA among taxonomically heterogeneous bacterioplankton, and suggest a wide distribution of functional gene(i.e., dmdA) in global marine environments. | Yinxin Zeng | 2019 | Acta Oceanologica Sinica2019，38，8： | 3 |
| 7 | Diversity of the aerobic anoxygenic phototrophy gene pufM in Arctic and Antarctic coastal seawaters显示文摘Aerobic anoxygenic phototrophic(AAP) bacteria serve important functions in marine carbon and energy cycling because of their capability to utilize dissolved organic substrates and harvest light energy.AAP bacteria are widely distributed in marine environments,and their diversity has been examined in marine habitats.However,information about AAP bacteria at high latitudes remains insufficient to date.Therefore,this study determined the summer AAP bacterial diversity in Arctic Kongsfjorden and in the Antarctic coastal seawater of King George Island on the basis of puf M,a gene that encodes a pigment-binding protein subunit of the reaction center complex.Four puf M clone libraries were constructed,and 674 positive clones were obtained from four investigated stations(two in Kongsfjorden and two in the Antarctic Maxwell Bay).Arctic clones were clustered within the Alphaproteobacteria,whereas Antarctic clones were classified into the Alphaproteobacteria and Betaproteobacteria classes.Rhodobacteraceae-like puf M genes dominated in all samples.In addition,sequences closely related to puf M encoded on a plasmid in Sulfitobacter guttiformis were predominant in both Arctic and Antarctic samples.This result indicates the transpolar or even global distribution of puf M genes in marine environments.Meanwhile,differences between the Arctic and Antarctic sequences may prove polar endemism.These results indicate the important role of Rhodobacteraceae as AAP bacteria in bipolar coastal waters. | ZENG Yinxin DONG Peiyan QIAO Zongyun ZHENG Tianling | 2016 | Acta Oceanologica Sinica2016，35，6： | 3 |
| 8 | CAPPh A Cytoskeleton-Based Localization Assay Reports Protein-Protein Interaction in Living Cells by Fluorescence Microscopy显示文摘 | Sulian Lv Huiying Miao Ming Luo Yinxin Li Qiaomei Wang Yuh-Ru Julie Lee Bo Liu | 2017 | Molecular Plant2017，10，11： | 2 |
| 9 | Genome shuffling leads to rapid phenotypic improvement in bacteria显示文摘 | ZHANG Yinxin PERRY K VINCI V A | 2002 | Nature2002，415，： | 1 |
| 10 | 显示文摘 | YINXin(殷馨) DAIYuan-jing(戴媛静) | | 化学推进剂与高分子材料0，，： | 1 |
| 11 | Changes in DNA base sequences in the mutant of Arabidopsis thaliana induced by low-energy N+ implantation显示文摘 | Fengqi Chang Xuanming Liu Yinxin Li Gengxiang Jia Jingjing Ma Gongshe Liu Zhiqing Zhu | 2003 | Science in China Series C: Life Sciences2003，，5： | 1 |
| 12 | Human activity during the late Pleistocene in the Lop Nur region, northwest China: Evidence from a buried stone artifact显示文摘The Lop Nur region, in the east part of Tarim Basin, was an important transportation junction between west and east,north and south Eurasia. However, previous studies on prehistoric human activity have concentrated mostly on the Bronze Age,whereas that during the Stone Age remains largely unresearched. Here, we present a new direct evidence of human activity in the late Pleistocene, recorded on a grinding stone buried in a lacustrine sediment section of the Lop Nur region. The grain size distribution of the sediment section indicates that the site was probably in the center of a lake with weak hydrodynamic environment. Therefore, the stone artifact can only be carried to here by people instead of river and it was never move as soon as left here. Results of radiocarbon dating, the evident stratigraphic relations between the stone artifact and the sedimentary formation, indicate that the human activity could extend to approximately 13 ka BP. Furthermore, the results of starch-grain and use-wear analyses suggest that ancient humans gathered seeds of Triticeae, roots, and tubers and used the grinding stone to simply process selected plant as plant foodstuffs during this period. It implies that the environmental conditions in the river delta of the Lop Nur were inhabitable during the late Pleistocene. | Kangkang LI Xiaoguang QIN Xiaoyan YANG Bing XU Lei ZHANG Guijin MU Dong WEI Chunxue WANG Yong WU Xiaohong TIAN Yongchong LIN Wen LI Jiaqi LIU Yinxin JIAO | 2018 | Science China Earth Sciences2018，61，11： | 1 |
| 13 | Performance and stability of yield in response to plant density,year and location in maize hybrids of Northwest China显示文摘Identifying desirable genotypes with the best performance in diverse environments is a perpetual aim of plant breeding,and the interaction between genotype and environment(G×E)always plays a key role.This study was conducted to elucidate the genetic behaviour of different hybrid combinations at various densities in diverse environments.According to a line×tester design,32 hybrid combinations were obtained from 16 inbred individuals crossed with two testers and planted at three locations at three density levels(45,000,67,500 and 90,000 plants ha-1)during 2014-2017.Genotype(G),environment(E)and the interaction of genotype and environment(G×E)significantly affected grain yield at different densities.Increasing planting density enhanced grain yield and improved the efficiency of germplasm screening,where the effect of location on grain yield at different densities was larger than that of year and the GCA was larger than the SCA.Finally,four inbred lines(KB102,KB081,KA105,and KB106)with a high GCA,environmental adaptability,and several combinations using them as parents have been approved in ShannXi Province and National of China.In conclusion,the evaluation of combining ability at multiple densities and locations can effectively screen inbred lines and improve breeding efficiency. | Yuan Dong Zhiqian Feng Ting Li Ali Farhan Xiaokang Tian Yonghui Lao Yinxin Duan Leiyong Si Xinghua Zhang Jiquan Xue Shutu Xu | 2022 | Technology in Agronomy2022，2，1： | 1 |
| 14 | Molecular cloning and characterization of a Na^+/H^+ antiporter gene in halophyte Tetragonia tetragonioides显示文摘 | Huiying L Yinxin L I Hua C | 2004 | Chin High Technol Lett2004，14，11： | 1 |
| 15 | Microstructure and dielectric properties of Ba x Sr 1? x TiO 3 ceramics显示文摘 | Chunlin Fu Chuanren Yang Hongwei Chen Yinxin Wang Liye Hu | 2005 | Materials Science & Engineering B2005，，2： | 1 |
| 16 | Development of an efficient protocol of RNA isolation from recalcitrant tree tissues显示文摘 | Xuchu Wang Weimin Tian Yinxin Li | 2008 | Molecular biotechnology2008，38，1： | 1 |
| 17 | Optimal planning of distribution substation locations and sizes-model and algorithm显示文摘 | Yu Yinxin Huang Chunhua | 1996 | International Journal of Electrical Power and Energy System1996，18，6： | 1 |
| 18 | Overexpression ofphytoene synthase gene from Salicorniaeuropaea alters response to reactive oxygen species under salt stress in transgenic Arabidopsis显示文摘 | Han Heping Li Yinxin Zhou Shufeng | 2008 | Biotechnol Lett2008，30，8： | 1 |
| 19 | Phylogenetic diversity and phenotypic characterization of cultivable bacterioplankton isolated from polar oceans显示文摘A set of 27 marine planktonic bacteria isolated from the polar regions was characterized by 16S rDNA sequencing and physiological and biochemical testing. More than half of these bacteria were positive for caseinase, gelatinase and β-glucosidase, and could utilize glucose, maltose or malic acid as carbon source for cell growth. Twelve isolates expressed nitrate reduction activities. Except for one antarctic isolate BSw10175 belonging to Actinobacteria phylum, these isolates were classified as γ-Proteobacteria, suggesting that γ-Proteobacteria dominated in cultivable marine bacterioplankton at both poles. Genus Pseudoalteromonas was the predominant group in the Chukchi Sea and the Bering Sea, and genus Shewanella dominated in cultivable bacterioplankton in the Prydz Bay. With sequence similarities above 97%, genus Psychrobacter was found at both poles. These 27 isolates were psychrotolerant, and significant 16S rDNA sequence similarities were found not only between arctic and antarctic marine bacteria (>99%), but also between polar marine bacteria and bacteria from other aquatic environments (≥98.8%), including temperate ocean, deep sea, pond and lake, suggesting that in the polar oceans less temperature-sensitive bacteria may be cosmopolitan and have a bipolar, even global, distribution at the species level. | ZENG Yinxin LI Huirong YU Yong CHEN Bo ZHENG Tianling | 2007 | Acta Oceanologica Sinica2007，26，4： | 1 |
| 20 | miR-92a is a critical regulator of the apoptosis pathway in glioblastomawith inverse expression of BCL2L11显示文摘 | Huanjiang Niu Kun Wang Anling Zhang Shuxu Yang Zhengfei Song Wei Wang Cong Qian Xinwei Li Yinxin Zhu Yirong Wang | 2012 | Oncology Reports2012，，5： | 1 |
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