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| 1 | Genome-wide analysis of microRNAs induced and regulated by colchicine in mulberry显示文摘A variety of plants were colchicine treated to double their chromosome number.Chromosomes are genetic carriers that determine the individual traits of organisms.The doubling of chromosomes will lead to modifications in plant morphology,physiology and genetics.To determine the response of mulberry trees induced by colchicine,using mulberry variety Yu-711 leaves as research materials,two small RNA libraries(control and experimental groups)were constructed.It was found that 45 known miRNA genes and 78 predicted novel miRNA genes in the sequence results.A comparison of data between the control group and the experimental group revealed 37 differentially expressed miRNA genes,19 genes of which were up-regulated and 18 genes of which were down-regulated.Eight miRNAs were selected from 37 differentially expressed miRNA genes.These miRNAs were verified by quantitative real-time PCR,and the expression levels of these miRNAs were found to be consistent with those obtained by sequencing,which proved the accuracy of sequencing results.The PsRNATarget software was used to predict the target genes of 8 miRNAs.The Gene Ontology and Kyoto Encycopedia of Genes and Genomes analysis were used to collect the functions of target genes and confirm that target genes are mainly involved in biological processes,cell components and molecular functions.novel-77 miRNA was selected from 37 differentially expressed miRNAs.What will serve as a fundamental data in understanding mulberry miRNAs function in molecular biology research and further provides the molecular mechanism of mulberry gene regulation as induced by colchicine is that the prediction of novel_77 precursor sequence and the target gene(XM_010104258.2),as well as the secondary structure analysis. | MENGMENG WU PENG GUO YISU SHI DANYAN ZHENG QIAONAN ZHANG XIN JIN YUHUA WANG QIANG LIN RONGJUN FANG WEIGUO ZHAO | 2022 | BIOCELL2022，46，8： | 0 |
| 2 | T cell cross-reactivity in autoimmune-like hepatitis triggered by COVID-19显示文摘Over 1000 cases of pediatric hepatitis of unknown etiology have been reported worldwide since the first case was reported in the UK.To date,the etiology of pediatric hepatitis remains unknown and controversial.Adenovirus was first suspected to be the cause as it was present in the blood samples of the majority of cases.Partial cases have also been tested positive for severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)[1].However,it is still unclear how these viruses contribute to pediatric hepatitis.In the case of a pediatric patient with SARS-CoV-2 infection,the liver biopsy showed acute submassive hepatocyte necrosis,accompanied by a significant increase in T cell infiltration[2].Furthermore,CD8+T cell dominant hepatitis induced by coronavirus disease 2019(COVID-19)vaccination has also been recently reported[3].Although it is known that T cell receptors(TCRs)can discriminate between self-and non-self-antigens,it is now well-accepted that TCRs exhibit cross-reactivity toward similar and even distinct antigen peptides[4].Thus,we hypothesized that following SARS-CoV-2 infection or vaccination,T cells carrying TCRs that recognize self-antigens undergo clonal expansion,which could eventually result in the onset of autoimmune-like hepatitis(Figure 1A). | Yisu Liu Yuqian Wang Zhiqiang Peng Guideng Li Jianwei Wang | 2023 | hLife2023，1，1： | 0 |
| 3 | Response of MaHMA2 gene expression and stress tolerance to zinc stress in mulberry(Morus alba L.)显示文摘HMA2(heavy metal ATPase 2)plays a crucial role in extracellular and intracellular Zn^(2+)transport across biomembranes,maintaining ion homeostasis,and playing an important role in the normal physiological metabolism,growth,and development of plants.In our study,a novel HMA2 gene,named MaHMA2,was isolated and cloned from white mulberry(Morus alba L.).The gene sequence obtained was 1,342 bp long,with an open reading frame of 1,194 bp,encoding a protein of 397 amino acids,with a predicted molecular mass of 42.852 kD and an isoelectric point of 7.53.This protein belonged to the PIB-type ATPase transport protein family.We analyzed the expression of the MaHMA2 gene by quantitative real-time PCR.The results showed that the level of MaHMA2 gene expression decreased to a Zn concentration of 800 mg/kg.Malondialdehyde and proline levels increased and responded to increasing Zn when the MaHMA2 gene was silenced,whereas the activities of peroxidase and superoxide dismutase tended to increase in response to increasing Zn^(2+)ion stress concentrations but were lower in the gene-silenced plants.These findings suggested that the MaHMA2 gene played an active role in the tolerance response of mulberry to Zn stress. | LEI WANG QIUXIA DU YISU SHI MICHAEL ACKAH PENG GUO DANYAN ZHENG MENGMENG WU XIN JIN PEILAN LI QIAONAN ZHANG RUIXUE LI ZHI YIN MENGDI ZHAO WEIGUO ZHAO | 2022 | BIOCELL2022，46，10： | 0 |
| 4 | All-perfluoropolymer,nonlinear stability-assisted monolithic surface combines topology-specific superwettability with ultradurability显示文摘Developing versatile and robust surfaces that mimic the skins of living beings to regulate air/liquid/solid matter is critical for many bioinspired applications.Despite notable achievements,such as in the case of developing robust superhydrophobic surfaces,it remains elusive to realize simultaneously topology-specific superwettability and multipronged durability owing to their inherent tradeoff and the lack of a scalable fabrication method.Here,we present a largely unexplored strategy of preparing an all-perfluoropolymer(Teflon),nonlinear stability-assisted monolithic surface for efficient regulating matters. | Wanbo Li Chiu-wing Chan Zeyu Li Sin-Yung Siu Siyu Chen Han Sun Zeyu Liu Yisu Wang Chong Hu Nicola Maria Pugno Richard N.Zare Hongkai Wu Kangning Ren | 2023 | The Innovation2023，4，2： | 0 |
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