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| 1 | Nitrative and oxidative DNA damage in intrahepatic cholangiocarcinoma patients in relation to tumor invasion显示文摘AIM: Nitrative and oxidative DNA damage such as 8-nitroguanine and 8-oxo-7,8-dihydro-2'-deoxyguanosine(8-oxodG) formation has been implicated in initiation and/or promotion of inflammation-mediated carcinogenesis.The aim of this study is to clarify whether these DNA lesions participate in the progression of intrahepatic cholangiocarcinoma.METHODS: We investigated the relation of the formation of 8-nitroguanine and 8-oxodG and the expression of hypoxia-inducible factor-1α (HIF-1α) with tumor invasion in 37 patients with intra-hepatic cholangiocarcinoma.RESULTS: Immunohistochemical analyses revealed that 8-nitroguanine and 8-oxodG formation occurred to a much greater extent in cancerous tissues than in non-cancerous tissues. HIF-1α could be detected in cancerous tissues in all patients, suggesting low oxygen tension in the tumors.HIF-1α expression was correlated with inducible nitric oxide synthase (iNOS) expression (r= 0.369 and P = 0.025)and 8-oxodG formation (r = 0.398 and P = 0.015).Double immunofluorescence study revealed that iNOS and HIF-1α co-localized in cancerous tissues. Notably, the formation of 8-oxodG was correlated significantly with lymphatic invasion (r = 0.386 and P= 0.018). Moreover, 8-nitroguanine and 8-oxodG in non-cancerous tissues were associated significantly with neural invasion (P = 0.042and P = 0.026, respectively). These results suggest that reciprocal activation between HIF-1α and iNOS mediates persistent DNA damage, which induces tumor invasiveness via mutations, resulting in poor prognosis.CONCLUSION: The formation of 8-nitroguanine and 8-oxodG plays an important role in multiple steps of genetic changes leading to tumor progression, including invasiveness. | Somchai Pinlaor Banchob Sripa Ning Ma Yusuke Hiraku Puangrat Yongvanit Sopit Wongkham Chawalit Pairojkul Vajarabhongsa Bhudhisawasdi Shinji Oikawa Mariko Murata Reiji Semba Shosuke Kawanishi | 2005 | World Journal of Gastroenterology2005，11，30： | 9 |
| 2 | Annexin A1: A new immunohistological marker of cholangiocarcinoma显示文摘AIM: To evaluate a new immunohistological marker, annexin A1 (ANXA1), in cholangiocarcinoma (CCA) and hepatocellular carcinoma (HCC). METHODS: Expression of ANXA1 protein was investigated in liver tissues from patients with CCA and HCC by immunohistochemistry. Its expression on differences stages of tumor development was investigated in hamster CCA tissues induced by Opisthorchis viverrini and N -nitrosodimethylamine. Moreover, mRNA expression of ANXA1 was assessed in CCA cell lines by quantitative real-time polymerase chain reaction and silencing of ANXA1 gene expression using small interfering RNA. RESULTS: In human CCA tissue arrays, immunohistochemical analysis revealed that the positive expression of ANXA1 was 94.1% (64/68 cases) consisting of a high expression (66.2%, 45/68 cases) and a low expression (33.8%, 23/68 cases). However, expression of ANXA1 protein was negative in all histologic patterns for HCC (46/46 cases) and healthy individuals (6/6 cases). In hamster with opisthorchiasis-associated CCA, the expression of ANXA1 was observed in the cytoplasm of inflammatory cells, bile duct epithelia and tumor cells. Grading scores of ANXA1 expression were significantly increased with tumor progression. In addition, mRNA expression of ANXA1 significantly increased in all of the various CCA cell lines tested compared to an immortalized human cholangiocyte cell line (MMNK1). Suppressing the ANXA1 gene significantly reduced the matrix metalloproteinase (MMP) 2 and MMP9, and transforming growth factor-β genes, but increased nuclear factor-kB gene expression. CONCLUSION: ANXA1 is highly expressed in CCA, but low in HCC, suggesting it may serve as a new immunohistochemical marker of CCA. ANXA1 may play a role in opisthorchiasis-associated cholangiocarcinogenesis. | Nuttanan Hongsrichan Rucksak Rucksaken Yaovalux Chamgramol Porntip Pinlaor Anchalee Techasen Puangrat Yongvanit Narong Khuntikeo Chawalit Pairojkul Somchai Pinlaorr | 2013 | World Journal of Gastroenterology2013，19，16： | 4 |
| 3 | 8-nitroguanine formation in the liver of hamsters infected with Opisthorchis viverrini显示文摘 | Pinlaor S Yongvanit P Hiraku Y | 2003 | Biochemical and Biophysical Research Communications2003，309，3： | 1 |
| 4 | Induction of cytochrome P450 2A6 expression in humans by the carcinogenic parasite infection,opisthorchiasis viverrini显示文摘 | Lang M A Yongvanit P | 1996 | Cancer Epidemiol Biomarkers Prev1996，5，10： | 1 |
| 5 | iNOS-dependent DNA damage via NF-kappa B expression in hamsters infected with opisthorchis viverrini and its suppression by the antihel minthic drug praziquantel显示文摘 | Pinlaor S Hiraku Y Yongvanit P | 2006 | Int J Cancer2006，119，5： | 1 |
| 6 | Urinary microRNA-192and microRNA-21as potential indicators for liver flukeassociated cholangiocarcinoma risk group显示文摘 | Silakit R Loilome W Yongvanit P | 2015 | Parasitol Int2015，，15： | 1 |
| 7 | High expres- sion of HIF- 1 ct, BNIP3 and PI3KC3: hypoxia-induced auto- phagy predicts cholangiocarcinoma survival and metastasis 显示文摘 | Thongchot S Yongvanit P Loilome W | 2014 | Asian Pac J Cancer Prev2014，15，14： | 1 |
| 8 | Roles of liver fluke infection as risk factor for cholangiocarcinoma显示文摘 | Sithithaworn P Yongvanit P Duenngai K | 2014 | J Hepatobiliary Pancreat Sci2014，21，5： | 1 |
| 9 | 8-nitroguanine formation in the liver of hamsters infected with Opisthorchis viverrini 显示文摘 | Pinlaor S Yongvanit P Hiraku Y | 2003 | Biochem Biophys Res Commun2003，309，3： | 1 |
| 10 | Genetic and environmental determinants of risk for cholangiocarcinoma in Thailand显示文摘Cholangiocarcinoma(CCA) is a difficult cancer to diagnose in the early stage and to treat by curative resec-tion. The incidence of CCA in the northeast of Thailand is the highest in the world. To make progress in detecting a high risk group and in the prevention and detection of CCA, we have been analyzing the risk factors for CCA. Although liver fluke infection is known to be a risk factor, there are patients who are not infected with the liver fluke and not all people infected with the liver fluke will suffer from the disease. Therefore, it is of the utmost importance to analyze the risk factors and the mechanism to prevent the disease and also to detect the disease in its early stage to save patients' lives. Through collaboration among Thai and Japanese researchers, we analyzed the genetic and environmental determinants of risks for CCA. Also, we have been trying to develop methods to detect the disease in a non-invasive way. Without repeating findings reported in various reviews on CCA, we will first discuss the environmental and genetic determinants of the risks for CCA. Second, we will discuss the properties of CCA, including the etiological agents and the mechanism of cholangiocarcinogenesis, and finally, we will discuss future approaches to prevent and cure CCA from the standpoint of evidence-based medicine. We will discuss these points by including the data from our laboratories. We would like to emphasize the importance of the genetic data, especially whole genome approaches, to understand the properties of CCA, to find a high risk population for CCA and to develop effective preventative methods to stop the carcinogenic steps toward CCA in the near future. In addition, it is of the upmost importance to develop a non-invasive, specific and sensitive method to detect CCA in its early stage for the application of modern medical approaches to help patients with CCA. | Masanao Miwa Satoshi Honjo Gyokukou You Masakazu Tanaka Kazuhiko Uchida Petcharin Srivatanakul Thiravud Khuhaprema Watcharin Loilome Anchalee Techasen Chaisiri Wongkham Temduang Limpaiboon Puangrat Yongvanit Sopit Wongkham | 2014 | World Journal of Gastrointestinal Pathophysiology2014，5，4： | 1 |
| 11 | Curcumin decreases cholangiocarcinogenesis in hamsters by suppressing inflammation-mediated molecular events related to multistep cardnogenesis 显示文摘 | Prakobwong S Khoontawad J Yongvanit P | 2011 | Int J Cancer2011，129，1： | 1 |
| 12 | Mechanisms of ox ysterol- induced carcino- genesis显示文摘 | Jusakul A Yongvanit P Loilome W | 2011 | Lipids Health Dis2011，10，： | 1 |
| 13 | 8-nitroguanine formation in the liver of hamsters infected with opisthorchis viverrin显示文摘 | Somchai pinlaor Puangrat Yongvanit Yusuke Hiraku | 2003 | J Biophys Res Commun2003，309，3： | 1 |
| 14 | High expression of HIF-I c, BNIP3 and PI3KC3 : hypoxia-induced autophagy predicts cholangiocarcinoma survival and metastasis显示文摘 | Thongehot S Yongvanit P Loilome W | 2014 | Asian Pac J Canc- er Prey2014，15，14： | 1 |
| 15 | BMP-7 blocks the effects of TGF-β-induced EMT in cholangiocarcinoma显示文摘 | Kassaporn Duangkumpha Anchalee Techasen Watcharin Loilome Nisana Namwat Raynoo Thanan Narong Khuntikeo Puangrat Yongvanit | 2014 | Tumor Biology2014，，10： | 1 |
| 16 | Curcumin induces a nuclear factor-erythroid 2-related factor 2-driven response against oxidative and nitrative stress after praziquantel treatment in liver fluke-infected hamsters显示文摘 | Lakhanawan Charoensuk Porntip Pinlaor Suksanti Prakobwong Yusuke Hiraku Umawadee Laothong Wipaporn Ruangjirachuporn Puangrat Yongvanit Somchai Pinlaor | 2011 | International Journal for Parasitology2011，，6： | 1 |
| 17 | Roles of liver fluke infection as risk factor for cholangiocarcinoma 显示文摘 | Sithithaworn P Yongvanit P Duenngai K | 2014 | J Hepatobiliary Pancreat Sci2014，21，5： | 1 |
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